The Experts below are selected from a list of 20229 Experts worldwide ranked by ideXlab platform
Cassandra L. Smith - One of the best experts on this subject based on the ideXlab platform.
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a contiguous not i Restriction Map of band q22 3 of human chromosome 21
Proceedings of the National Academy of Sciences of the United States of America, 1992Co-Authors: Denan Wang, Hong Fang, Charles R Cantor, Cassandra L. SmithAbstract:Abstract A contiguous high-resolution NotI Restriction Map of the distal region of the long arm of human chromosome 21 was constructed by three strategies: linking clones to identify adjacent pieces of DNA, partial digestion to identify neighboring fragments, and cell line polymorphisms to prove identity or adjacency of DNA fragments. Twenty-nine single-copy DNA probes and five linking clone probes were used to determine the order of 30 Not I fragments, covering 10 megabases of DNA in band q22.3. Smaller Not I fragments occur preferentially in this region, suggesting that band q22.3 is unusually rich in genes, since Not I sites occur almost exclusively in CpG islands. Comparison of the physical Map and genetic Maps in this region reveals a 10-fold higher than average recombination frequency.
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Alignment of Sfi I sites with the Not I Restriction Map of Schizosaccharomyces pombe genome
Nucleic acids research, 1991Co-Authors: Jian-bing Fan, Dietmar Grothues, Cassandra L. SmithAbstract:Abstract A Sfi I Restriction Map of the fission yeast Schizosaccharomyces pombe genome was aligned with the Not I Restriction Map. There are 16 Sfi I sites in the S. pombe genome. Three Sfi I sites are on chromosome III which is devoid of Not I sites. The sizes of the entire genome and individual chromosomes, calculated from the Sfi I fragment sizes, are consistent with that calculated from the Not I fragment sizes. The Sfi I Map provides greater physical characterization of the S. pombe genome and further validates the use of S. pombe chromosomal DNA as size standard. These Maps have allowed detection of polymorphism on all three chromosomes.
Jian-bing Fan - One of the best experts on this subject based on the ideXlab platform.
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Alignment of Sfi I sites with the Not I Restriction Map of Schizosaccharomyces pombe genome
Nucleic acids research, 1991Co-Authors: Jian-bing Fan, Dietmar Grothues, Cassandra L. SmithAbstract:Abstract A Sfi I Restriction Map of the fission yeast Schizosaccharomyces pombe genome was aligned with the Not I Restriction Map. There are 16 Sfi I sites in the S. pombe genome. Three Sfi I sites are on chromosome III which is devoid of Not I sites. The sizes of the entire genome and individual chromosomes, calculated from the Sfi I fragment sizes, are consistent with that calculated from the Not I fragment sizes. The Sfi I Map provides greater physical characterization of the S. pombe genome and further validates the use of S. pombe chromosomal DNA as size standard. These Maps have allowed detection of polymorphism on all three chromosomes.
John Valdes - One of the best experts on this subject based on the ideXlab platform.
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a cosmid contig and high resolution Restriction Map of the 2 megabase region containing the huntington s disease gene
Nature Genetics, 1993Co-Authors: Sarah Baxendale, Marcy E Macdonald, Richard Mott, Fiona Francis, Susan Kirby, Marianne James, Gunther Zehetner, Holger Hummerich, John Valdes, Francis S CollinsAbstract:A cosmid contig and high resolution Restriction Map of the 2 megabase region containing the Huntington's disease gene
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A cosmid contig and high resolution Restriction Map of the 2 megabase region containing the Huntington's disease gene.
Nature genetics, 1993Co-Authors: Sarah Baxendale, Marcy E Macdonald, Richard Mott, Fiona Francis, Marianne James, Gunther Zehetner, Holger Hummerich, Carol Lin, Susan F. Kirby, John ValdesAbstract:The quest for the mutation responsible for Huntington's disease (HD) has required an exceptionally detailed analysis of a large part of 4p16.3 by molecular genetic techniques, making this stretch of 2.2 megabases one of the best characterized regions of the human genome. Here we describe the construction of a cosmid and P1 clone contig spanning the region containing the HD gene, and the establishment of a detailed, high resolution Restriction Map. This ordered clone library has allowed the identification of several genes from the region, and has played a vital role in the recent identification of the Huntington's disease gene. The Restriction Map provides the framework for the detailed analysis of a region extremely rich in coding sequences. This study also exemplifies many of the strategies to be used in the analysis of larger regions of the human genome.
Sarah Baxendale - One of the best experts on this subject based on the ideXlab platform.
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a cosmid contig and high resolution Restriction Map of the 2 megabase region containing the huntington s disease gene
Nature Genetics, 1993Co-Authors: Sarah Baxendale, Marcy E Macdonald, Richard Mott, Fiona Francis, Susan Kirby, Marianne James, Gunther Zehetner, Holger Hummerich, John Valdes, Francis S CollinsAbstract:A cosmid contig and high resolution Restriction Map of the 2 megabase region containing the Huntington's disease gene
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A cosmid contig and high resolution Restriction Map of the 2 megabase region containing the Huntington's disease gene.
Nature genetics, 1993Co-Authors: Sarah Baxendale, Marcy E Macdonald, Richard Mott, Fiona Francis, Marianne James, Gunther Zehetner, Holger Hummerich, Carol Lin, Susan F. Kirby, John ValdesAbstract:The quest for the mutation responsible for Huntington's disease (HD) has required an exceptionally detailed analysis of a large part of 4p16.3 by molecular genetic techniques, making this stretch of 2.2 megabases one of the best characterized regions of the human genome. Here we describe the construction of a cosmid and P1 clone contig spanning the region containing the HD gene, and the establishment of a detailed, high resolution Restriction Map. This ordered clone library has allowed the identification of several genes from the region, and has played a vital role in the recent identification of the Huntington's disease gene. The Restriction Map provides the framework for the detailed analysis of a region extremely rich in coding sequences. This study also exemplifies many of the strategies to be used in the analysis of larger regions of the human genome.
Dietmar Grothues - One of the best experts on this subject based on the ideXlab platform.
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Alignment of Sfi I sites with the Not I Restriction Map of Schizosaccharomyces pombe genome
Nucleic acids research, 1991Co-Authors: Jian-bing Fan, Dietmar Grothues, Cassandra L. SmithAbstract:Abstract A Sfi I Restriction Map of the fission yeast Schizosaccharomyces pombe genome was aligned with the Not I Restriction Map. There are 16 Sfi I sites in the S. pombe genome. Three Sfi I sites are on chromosome III which is devoid of Not I sites. The sizes of the entire genome and individual chromosomes, calculated from the Sfi I fragment sizes, are consistent with that calculated from the Not I fragment sizes. The Sfi I Map provides greater physical characterization of the S. pombe genome and further validates the use of S. pombe chromosomal DNA as size standard. These Maps have allowed detection of polymorphism on all three chromosomes.
