The Experts below are selected from a list of 9 Experts worldwide ranked by ideXlab platform
Ubaldo Armato - One of the best experts on this subject based on the ideXlab platform.
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de novo engineering of Reticular Connective Tissue in vivo by silk fibroin nonwoven materials
Biomaterials, 2005Co-Authors: Ilaria Pierpaola Dal Pra, Giuliano Freddi, Jasminka Minic, Anna Maria Chiarini, Ubaldo ArmatoAbstract:Biologically tolerated biomaterials are the focus of intense research. In this work, we examined the biocompatibility of three-dimensional (3D) nonwovens of sericin-deprived, Bombyx mori silk fibroin (SF) in beta-sheet form implanted into the subcutaneous Tissue of C57BL6 mice, using sham-operated mice as controls. Both groups of mice similarly healed with no residual problem. Macroarray analysis showed that an early (day 3) transient expression of macrophage migration inhibitory factor (MIF) mRNA, but not of the mRNAs encoding for 22 additional proinflammatory cytokines, occurred solely at SF-grafted places, where no remarkable infiltration of macrophages or lymphocytes subsequently happened. Even an enduring moderate increase in total cytokeratins without epidermal hyperkeratosis and a transient (days 10-15) upsurge of vimentin occurred exclusively at SF-grafted sites, whose content of collagen type-I, after a delayed (day 15) rise, ultimately fell considerably under that proper of sham-operated places. By day 180, the interstices amid and surfaces of the SF chords, which had not been appreciably biodegraded, were crammed with a newly produced Tissue histologically akin to a vascularized Reticular Connective Tissue, while some macrophages but no lymphocytic infiltrates or fibrous capsules occurred in the adjoining Tissues. Therefore, SF nonwovens may be excellent candidates for clinical applications since they both enjoy a long-lasting biocompatibility, inducing a quite mild foreign body response, but no fibrosis, and efficiently guide Reticular Connective Tissue engineering.
Th Krieg - One of the best experts on this subject based on the ideXlab platform.
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ultrastructural binding sites of endomysium antibodies from sera of patients with dermatitis herpetiformis and coeliac disease
Gut, 1992Co-Authors: Sarolta Kárpáti, Marita Meurer, Wilhelm Stolz, A Burginwolff, Otto Braunfalco, Th KriegAbstract:The ultrastructural binding sites of endomysium antibodies, specific serological markers of gluten sensitive enteropathy, were investigated in the rabbit oesophagus using the immunogold technique. Endomysium antibodies from sera of patients with dermatitis herpetiformis and with coeliac disease bound in an identical manner in a non-fibrillar material closely associated with fine collagenous-reticulin fibrils and also with similar fibrils connecting smooth muscle cells and elastic Tissue in the endomysial Connective Tissue. These observations suggest that IgA antibodies in sera from patients with dermatitis herpetiformis and coeliac disease recognise a common antigen in an amorphous component associated with the Reticular Connective Tissue of oesophageal lamina muscularis mucosae and thus confirm the probable identity of IgA class endomysium and jejunal antibodies.
Ilaria Pierpaola Dal Pra - One of the best experts on this subject based on the ideXlab platform.
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de novo engineering of Reticular Connective Tissue in vivo by silk fibroin nonwoven materials
Biomaterials, 2005Co-Authors: Ilaria Pierpaola Dal Pra, Giuliano Freddi, Jasminka Minic, Anna Maria Chiarini, Ubaldo ArmatoAbstract:Biologically tolerated biomaterials are the focus of intense research. In this work, we examined the biocompatibility of three-dimensional (3D) nonwovens of sericin-deprived, Bombyx mori silk fibroin (SF) in beta-sheet form implanted into the subcutaneous Tissue of C57BL6 mice, using sham-operated mice as controls. Both groups of mice similarly healed with no residual problem. Macroarray analysis showed that an early (day 3) transient expression of macrophage migration inhibitory factor (MIF) mRNA, but not of the mRNAs encoding for 22 additional proinflammatory cytokines, occurred solely at SF-grafted places, where no remarkable infiltration of macrophages or lymphocytes subsequently happened. Even an enduring moderate increase in total cytokeratins without epidermal hyperkeratosis and a transient (days 10-15) upsurge of vimentin occurred exclusively at SF-grafted sites, whose content of collagen type-I, after a delayed (day 15) rise, ultimately fell considerably under that proper of sham-operated places. By day 180, the interstices amid and surfaces of the SF chords, which had not been appreciably biodegraded, were crammed with a newly produced Tissue histologically akin to a vascularized Reticular Connective Tissue, while some macrophages but no lymphocytic infiltrates or fibrous capsules occurred in the adjoining Tissues. Therefore, SF nonwovens may be excellent candidates for clinical applications since they both enjoy a long-lasting biocompatibility, inducing a quite mild foreign body response, but no fibrosis, and efficiently guide Reticular Connective Tissue engineering.
Sarolta Kárpáti - One of the best experts on this subject based on the ideXlab platform.
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ultrastructural binding sites of endomysium antibodies from sera of patients with dermatitis herpetiformis and coeliac disease
Gut, 1992Co-Authors: Sarolta Kárpáti, Marita Meurer, Wilhelm Stolz, A Burginwolff, Otto Braunfalco, Th KriegAbstract:The ultrastructural binding sites of endomysium antibodies, specific serological markers of gluten sensitive enteropathy, were investigated in the rabbit oesophagus using the immunogold technique. Endomysium antibodies from sera of patients with dermatitis herpetiformis and with coeliac disease bound in an identical manner in a non-fibrillar material closely associated with fine collagenous-reticulin fibrils and also with similar fibrils connecting smooth muscle cells and elastic Tissue in the endomysial Connective Tissue. These observations suggest that IgA antibodies in sera from patients with dermatitis herpetiformis and coeliac disease recognise a common antigen in an amorphous component associated with the Reticular Connective Tissue of oesophageal lamina muscularis mucosae and thus confirm the probable identity of IgA class endomysium and jejunal antibodies.
Giuliano Freddi - One of the best experts on this subject based on the ideXlab platform.
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de novo engineering of Reticular Connective Tissue in vivo by silk fibroin nonwoven materials
Biomaterials, 2005Co-Authors: Ilaria Pierpaola Dal Pra, Giuliano Freddi, Jasminka Minic, Anna Maria Chiarini, Ubaldo ArmatoAbstract:Biologically tolerated biomaterials are the focus of intense research. In this work, we examined the biocompatibility of three-dimensional (3D) nonwovens of sericin-deprived, Bombyx mori silk fibroin (SF) in beta-sheet form implanted into the subcutaneous Tissue of C57BL6 mice, using sham-operated mice as controls. Both groups of mice similarly healed with no residual problem. Macroarray analysis showed that an early (day 3) transient expression of macrophage migration inhibitory factor (MIF) mRNA, but not of the mRNAs encoding for 22 additional proinflammatory cytokines, occurred solely at SF-grafted places, where no remarkable infiltration of macrophages or lymphocytes subsequently happened. Even an enduring moderate increase in total cytokeratins without epidermal hyperkeratosis and a transient (days 10-15) upsurge of vimentin occurred exclusively at SF-grafted sites, whose content of collagen type-I, after a delayed (day 15) rise, ultimately fell considerably under that proper of sham-operated places. By day 180, the interstices amid and surfaces of the SF chords, which had not been appreciably biodegraded, were crammed with a newly produced Tissue histologically akin to a vascularized Reticular Connective Tissue, while some macrophages but no lymphocytic infiltrates or fibrous capsules occurred in the adjoining Tissues. Therefore, SF nonwovens may be excellent candidates for clinical applications since they both enjoy a long-lasting biocompatibility, inducing a quite mild foreign body response, but no fibrosis, and efficiently guide Reticular Connective Tissue engineering.