The Experts below are selected from a list of 186 Experts worldwide ranked by ideXlab platform

Ying Peng - One of the best experts on this subject based on the ideXlab platform.

  • Ornithine-δ-Aminotransferase Inhibits Neurogenesis During Xenopus Embryonic Development.
    Investigative Opthalmology & Visual Science, 2015
    Co-Authors: Ying Peng, Sandra K. Cooper, Jay M. Mei, Shuwei Qiu, Gregory L. Borchert, Steven P. Donald, Hsiang-fu Kung, James M. Phang
    Abstract:

    PURPOSE In humans, deficiency of ornithine-δ-aminotransferase (OAT) results in progressive degeneration of the neural Retina (Gyrate Atrophy) with blindness in the fourth decade. In this study, we used the Xenopus embryonic developmental model to study functions of the OAT gene on embryonic development. METHODS We cloned and sequenced full-length OAT cDNA from Xenopus oocytes (X-OAT) and determined X-OAT expression in various developmental stages of Xenopus embryos and in a variety of adult tissues. The phenotype, gene expression of neural developmental markers, and enzymatic activity were detected by gain-of-function and loss-of-function manipulations. RESULTS We showed that X-OAT is essential for Xenopus embryonic development, and overexpression of X-OAT produces a ventralized phenotype characterized by a small head, lack of axial structure, and defective expression of neural developmental markers. Using X-OAT mutants based on mutations identified in humans, we found that substitution of both Arg 180 and Leu 402 abrogated both X-OAT enzymatic activity and ability to modulate the developmental phenotype. Neurogenesis is inhibited by X-OAT during Xenopus embryonic development. CONCLUSIONS Neurogenesis is inhibited by X-OAT during Xenopus embryonic development, but it is essential for Xenopus embryonic development. The Arg 180 and Leu 402 are crucial for these effects of the OAT molecule in development.

Sandra K. Cooper - One of the best experts on this subject based on the ideXlab platform.

  • Ornithine-δ-Aminotransferase Inhibits Neurogenesis During Xenopus Embryonic Development.
    Investigative Opthalmology & Visual Science, 2015
    Co-Authors: Ying Peng, Sandra K. Cooper, Jay M. Mei, Shuwei Qiu, Gregory L. Borchert, Steven P. Donald, Hsiang-fu Kung, James M. Phang
    Abstract:

    PURPOSE In humans, deficiency of ornithine-δ-aminotransferase (OAT) results in progressive degeneration of the neural Retina (Gyrate Atrophy) with blindness in the fourth decade. In this study, we used the Xenopus embryonic developmental model to study functions of the OAT gene on embryonic development. METHODS We cloned and sequenced full-length OAT cDNA from Xenopus oocytes (X-OAT) and determined X-OAT expression in various developmental stages of Xenopus embryos and in a variety of adult tissues. The phenotype, gene expression of neural developmental markers, and enzymatic activity were detected by gain-of-function and loss-of-function manipulations. RESULTS We showed that X-OAT is essential for Xenopus embryonic development, and overexpression of X-OAT produces a ventralized phenotype characterized by a small head, lack of axial structure, and defective expression of neural developmental markers. Using X-OAT mutants based on mutations identified in humans, we found that substitution of both Arg 180 and Leu 402 abrogated both X-OAT enzymatic activity and ability to modulate the developmental phenotype. Neurogenesis is inhibited by X-OAT during Xenopus embryonic development. CONCLUSIONS Neurogenesis is inhibited by X-OAT during Xenopus embryonic development, but it is essential for Xenopus embryonic development. The Arg 180 and Leu 402 are crucial for these effects of the OAT molecule in development.

Jay M. Mei - One of the best experts on this subject based on the ideXlab platform.

  • Ornithine-δ-Aminotransferase Inhibits Neurogenesis During Xenopus Embryonic Development.
    Investigative Opthalmology & Visual Science, 2015
    Co-Authors: Ying Peng, Sandra K. Cooper, Jay M. Mei, Shuwei Qiu, Gregory L. Borchert, Steven P. Donald, Hsiang-fu Kung, James M. Phang
    Abstract:

    PURPOSE In humans, deficiency of ornithine-δ-aminotransferase (OAT) results in progressive degeneration of the neural Retina (Gyrate Atrophy) with blindness in the fourth decade. In this study, we used the Xenopus embryonic developmental model to study functions of the OAT gene on embryonic development. METHODS We cloned and sequenced full-length OAT cDNA from Xenopus oocytes (X-OAT) and determined X-OAT expression in various developmental stages of Xenopus embryos and in a variety of adult tissues. The phenotype, gene expression of neural developmental markers, and enzymatic activity were detected by gain-of-function and loss-of-function manipulations. RESULTS We showed that X-OAT is essential for Xenopus embryonic development, and overexpression of X-OAT produces a ventralized phenotype characterized by a small head, lack of axial structure, and defective expression of neural developmental markers. Using X-OAT mutants based on mutations identified in humans, we found that substitution of both Arg 180 and Leu 402 abrogated both X-OAT enzymatic activity and ability to modulate the developmental phenotype. Neurogenesis is inhibited by X-OAT during Xenopus embryonic development. CONCLUSIONS Neurogenesis is inhibited by X-OAT during Xenopus embryonic development, but it is essential for Xenopus embryonic development. The Arg 180 and Leu 402 are crucial for these effects of the OAT molecule in development.

Shuwei Qiu - One of the best experts on this subject based on the ideXlab platform.

  • Ornithine-δ-Aminotransferase Inhibits Neurogenesis During Xenopus Embryonic Development.
    Investigative Opthalmology & Visual Science, 2015
    Co-Authors: Ying Peng, Sandra K. Cooper, Jay M. Mei, Shuwei Qiu, Gregory L. Borchert, Steven P. Donald, Hsiang-fu Kung, James M. Phang
    Abstract:

    PURPOSE In humans, deficiency of ornithine-δ-aminotransferase (OAT) results in progressive degeneration of the neural Retina (Gyrate Atrophy) with blindness in the fourth decade. In this study, we used the Xenopus embryonic developmental model to study functions of the OAT gene on embryonic development. METHODS We cloned and sequenced full-length OAT cDNA from Xenopus oocytes (X-OAT) and determined X-OAT expression in various developmental stages of Xenopus embryos and in a variety of adult tissues. The phenotype, gene expression of neural developmental markers, and enzymatic activity were detected by gain-of-function and loss-of-function manipulations. RESULTS We showed that X-OAT is essential for Xenopus embryonic development, and overexpression of X-OAT produces a ventralized phenotype characterized by a small head, lack of axial structure, and defective expression of neural developmental markers. Using X-OAT mutants based on mutations identified in humans, we found that substitution of both Arg 180 and Leu 402 abrogated both X-OAT enzymatic activity and ability to modulate the developmental phenotype. Neurogenesis is inhibited by X-OAT during Xenopus embryonic development. CONCLUSIONS Neurogenesis is inhibited by X-OAT during Xenopus embryonic development, but it is essential for Xenopus embryonic development. The Arg 180 and Leu 402 are crucial for these effects of the OAT molecule in development.

Gregory L. Borchert - One of the best experts on this subject based on the ideXlab platform.

  • Ornithine-δ-Aminotransferase Inhibits Neurogenesis During Xenopus Embryonic Development.
    Investigative Opthalmology & Visual Science, 2015
    Co-Authors: Ying Peng, Sandra K. Cooper, Jay M. Mei, Shuwei Qiu, Gregory L. Borchert, Steven P. Donald, Hsiang-fu Kung, James M. Phang
    Abstract:

    PURPOSE In humans, deficiency of ornithine-δ-aminotransferase (OAT) results in progressive degeneration of the neural Retina (Gyrate Atrophy) with blindness in the fourth decade. In this study, we used the Xenopus embryonic developmental model to study functions of the OAT gene on embryonic development. METHODS We cloned and sequenced full-length OAT cDNA from Xenopus oocytes (X-OAT) and determined X-OAT expression in various developmental stages of Xenopus embryos and in a variety of adult tissues. The phenotype, gene expression of neural developmental markers, and enzymatic activity were detected by gain-of-function and loss-of-function manipulations. RESULTS We showed that X-OAT is essential for Xenopus embryonic development, and overexpression of X-OAT produces a ventralized phenotype characterized by a small head, lack of axial structure, and defective expression of neural developmental markers. Using X-OAT mutants based on mutations identified in humans, we found that substitution of both Arg 180 and Leu 402 abrogated both X-OAT enzymatic activity and ability to modulate the developmental phenotype. Neurogenesis is inhibited by X-OAT during Xenopus embryonic development. CONCLUSIONS Neurogenesis is inhibited by X-OAT during Xenopus embryonic development, but it is essential for Xenopus embryonic development. The Arg 180 and Leu 402 are crucial for these effects of the OAT molecule in development.