The Experts below are selected from a list of 1752 Experts worldwide ranked by ideXlab platform
Seishi Kato - One of the best experts on this subject based on the ideXlab platform.
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Full-Length Transcriptome Analysis of Human Retina-Derived Cell Lines ARPE-19 and Y79 Using the Vector-Capping Method
2020Co-Authors: Mio Oshikawa, Chihiro Tsutsui, Tomoko Ikegami, Yuki Fuchida, Maki Matsubara, Shigeru Toyama, Ron Usami, Kuniyo Ohtoko, Seishi KatoAbstract:PURPOSE. To collect an entire set of full-length cDNA clones derived from human retina-derived Cell Lines and to identify full-length transcripts for retinal preferentially expressed genes. METHODS. The full-length cDNA libraries were constructed from a Retinoblastoma Cell Line, Y79, and a retinal pigment epithelium Cell Line, ARPE-19, using the vector-capping method, which generates a genuine full-length cDNA. By single-pass sequencing of the 5Ј-end of cDNA clones and subsequent mapping to the human genome, the authors determined their transcriptional start sites and annotated the cDNA clones. RESULTS. Of the 23,616 clones isolated from Y79-derived cDNA libraries, 19,229 full-length cDNA clones were identified and classified into 4808 genes, including genes of Ͼ10 kbp. Of the 7067 genes obtained from the Y79 and ARPE-19 libraries, the authors selected 72 genes that were preferentially expressed in the eye, of which 131 clones corresponding to 57 genes were fully sequenced. As a result, we discovered many variants that were produced by different transcriptional start sites, alternative splicing, and alternative polyadenylation. CONCLUSIONS. The bias-free, full-length cDNA libraries constructed using the vector-capping method were shown to be useful for collecting an entire set of full-length cDNA clones for these retinal Cell Lines. Full-length transcriptome analysis of these cDNA libraries revealed that there were, unexpectedly, many transcript variants for each gene, indicating that obtaining the full-length cDNA for each variant is indispensable for analyzing its function. The full-length cDNA clones (approximately 80,000 clones each for ARPE-19 and Y79) will be useful as a resource for investigating the human retina. (Invest Ophthalmol Vis Sci. 2011;52:6662-6670
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full length transcriptome analysis of human retina derived Cell Lines arpe 19 and y79 using the vector capping method
Investigative Ophthalmology & Visual Science, 2011Co-Authors: Mio Oshikawa, Chihiro Tsutsui, Tomoko Ikegami, Yuki Fuchida, Maki Matsubara, Shigeru Toyama, Ron Usami, Kuniyo Ohtoko, Seishi KatoAbstract:PURPOSE. To collect an entire set of full-length cDNA clones derived from human retina-derived Cell Lines and to identify full-length transcripts for retinal preferentially expressed genes. METHODS. The full-length cDNA libraries were constructed from a Retinoblastoma Cell Line, Y79, and a retinal pigment epithelium Cell Line, ARPE-19, using the vector-capping method, which generates a genuine full-length cDNA. By single-pass sequencing of the 5'-end of cDNA clones and subsequent mapping to the human genome, the authors determined their transcriptional start sites and annotated the cDNA clones. RESULTS. Of the 23,616 clones isolated from Y79-derived cDNA libraries, 19,229 full-length cDNA clones were identified and classified into 4808 genes, including genes of >10 kbp. Of the 7067 genes obtained from the Y79 and ARPE-19 libraries, the authors selected 72 genes that were preferentially expressed in the eye, of which 131 clones corresponding to 57 genes were fully sequenced. As a result, we discovered many variants that were produced by different transcriptional start sites, alternative splicing, and alternative polyadenylation. CONCLUSIONS. The bias-free, full-length cDNA libraries constructed using the vector-capping method were shown to be useful for collecting an entire set of full-length cDNA clones for these retinal Cell Lines. Full-length transcriptome analysis of these cDNA libraries revealed that there were, unexpectedly, many transcript variants for each gene, indicating that obtaining the full-length cDNA for each variant is indispensable for analyzing its function. The full-length cDNA clones (approximately 80,000 clones each for ARPE-19 and Y79) will be useful as a resource for investigating the human retina.
Roseline Godbout - One of the best experts on this subject based on the ideXlab platform.
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mitochondrial atp synthase alpha subunit gene amplified in a Retinoblastoma Cell Line maps to chromosome 18
Genes Chromosomes and Cancer, 1995Co-Authors: Wenjun Bie, Jeremy A Squire, Murray Fraser, Malcolm C Paterson, Roseline GodboutAbstract:The human Retinoblastoma Cell Line Y79 has multiple copies of the MYCN gene and the DEAD box gene DDXI. Both genes have been mapped to chromosome band 2p24. A third gene, encoding the alpha-subunit of mitochondrial ATP synthase (ATPSA), is also amplified in Y79. Here we report that there are at least four human mitochondrial ATPSA-related genes located on four different chromosomes. The ATPSA gene that is amplified in Y79 originates from chromosome 18. In Y79, the amplified copies of both the ATPSA and the MYCN genes are located on a homogeneously staining region (HSR) at chromosome band Ip34.
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amplification of the gene encoding the α subunit of the mitochondrial atp synthase complex in a human Retinoblastoma Cell Line
Gene, 1993Co-Authors: Roseline Godbout, Dwayne A Bisgrove, Louis H Honore, Rufus S DayAbstract:Abstract A cDNA clone encoding the precursor of the α-subunit of the human mitochondrial ATP synthase (F1-ATPS) complex was isolated from a library prepared from the poly(A)+RNA present in a Retinoblastoma (RB) Cell Line. Northern blot analysis of RNAs derived from a variety of transformed Cell Lines as well as from normal human fetal tissues indicated that RNA expression was significantly higher in two of the four RB Cell Lines analysed, Y79 (10- to 30-fold) and RB522A (3- to 8-fold), than in other Cell Lines or tissues. The increased mRNA level was apparently the result of gene amplification in Y79, but not in RB522A.
Mio Oshikawa - One of the best experts on this subject based on the ideXlab platform.
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Full-Length Transcriptome Analysis of Human Retina-Derived Cell Lines ARPE-19 and Y79 Using the Vector-Capping Method
2020Co-Authors: Mio Oshikawa, Chihiro Tsutsui, Tomoko Ikegami, Yuki Fuchida, Maki Matsubara, Shigeru Toyama, Ron Usami, Kuniyo Ohtoko, Seishi KatoAbstract:PURPOSE. To collect an entire set of full-length cDNA clones derived from human retina-derived Cell Lines and to identify full-length transcripts for retinal preferentially expressed genes. METHODS. The full-length cDNA libraries were constructed from a Retinoblastoma Cell Line, Y79, and a retinal pigment epithelium Cell Line, ARPE-19, using the vector-capping method, which generates a genuine full-length cDNA. By single-pass sequencing of the 5Ј-end of cDNA clones and subsequent mapping to the human genome, the authors determined their transcriptional start sites and annotated the cDNA clones. RESULTS. Of the 23,616 clones isolated from Y79-derived cDNA libraries, 19,229 full-length cDNA clones were identified and classified into 4808 genes, including genes of Ͼ10 kbp. Of the 7067 genes obtained from the Y79 and ARPE-19 libraries, the authors selected 72 genes that were preferentially expressed in the eye, of which 131 clones corresponding to 57 genes were fully sequenced. As a result, we discovered many variants that were produced by different transcriptional start sites, alternative splicing, and alternative polyadenylation. CONCLUSIONS. The bias-free, full-length cDNA libraries constructed using the vector-capping method were shown to be useful for collecting an entire set of full-length cDNA clones for these retinal Cell Lines. Full-length transcriptome analysis of these cDNA libraries revealed that there were, unexpectedly, many transcript variants for each gene, indicating that obtaining the full-length cDNA for each variant is indispensable for analyzing its function. The full-length cDNA clones (approximately 80,000 clones each for ARPE-19 and Y79) will be useful as a resource for investigating the human retina. (Invest Ophthalmol Vis Sci. 2011;52:6662-6670
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full length transcriptome analysis of human retina derived Cell Lines arpe 19 and y79 using the vector capping method
Investigative Ophthalmology & Visual Science, 2011Co-Authors: Mio Oshikawa, Chihiro Tsutsui, Tomoko Ikegami, Yuki Fuchida, Maki Matsubara, Shigeru Toyama, Ron Usami, Kuniyo Ohtoko, Seishi KatoAbstract:PURPOSE. To collect an entire set of full-length cDNA clones derived from human retina-derived Cell Lines and to identify full-length transcripts for retinal preferentially expressed genes. METHODS. The full-length cDNA libraries were constructed from a Retinoblastoma Cell Line, Y79, and a retinal pigment epithelium Cell Line, ARPE-19, using the vector-capping method, which generates a genuine full-length cDNA. By single-pass sequencing of the 5'-end of cDNA clones and subsequent mapping to the human genome, the authors determined their transcriptional start sites and annotated the cDNA clones. RESULTS. Of the 23,616 clones isolated from Y79-derived cDNA libraries, 19,229 full-length cDNA clones were identified and classified into 4808 genes, including genes of >10 kbp. Of the 7067 genes obtained from the Y79 and ARPE-19 libraries, the authors selected 72 genes that were preferentially expressed in the eye, of which 131 clones corresponding to 57 genes were fully sequenced. As a result, we discovered many variants that were produced by different transcriptional start sites, alternative splicing, and alternative polyadenylation. CONCLUSIONS. The bias-free, full-length cDNA libraries constructed using the vector-capping method were shown to be useful for collecting an entire set of full-length cDNA clones for these retinal Cell Lines. Full-length transcriptome analysis of these cDNA libraries revealed that there were, unexpectedly, many transcript variants for each gene, indicating that obtaining the full-length cDNA for each variant is indispensable for analyzing its function. The full-length cDNA clones (approximately 80,000 clones each for ARPE-19 and Y79) will be useful as a resource for investigating the human retina.
Tomoko Ikegami - One of the best experts on this subject based on the ideXlab platform.
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Full-Length Transcriptome Analysis of Human Retina-Derived Cell Lines ARPE-19 and Y79 Using the Vector-Capping Method
2020Co-Authors: Mio Oshikawa, Chihiro Tsutsui, Tomoko Ikegami, Yuki Fuchida, Maki Matsubara, Shigeru Toyama, Ron Usami, Kuniyo Ohtoko, Seishi KatoAbstract:PURPOSE. To collect an entire set of full-length cDNA clones derived from human retina-derived Cell Lines and to identify full-length transcripts for retinal preferentially expressed genes. METHODS. The full-length cDNA libraries were constructed from a Retinoblastoma Cell Line, Y79, and a retinal pigment epithelium Cell Line, ARPE-19, using the vector-capping method, which generates a genuine full-length cDNA. By single-pass sequencing of the 5Ј-end of cDNA clones and subsequent mapping to the human genome, the authors determined their transcriptional start sites and annotated the cDNA clones. RESULTS. Of the 23,616 clones isolated from Y79-derived cDNA libraries, 19,229 full-length cDNA clones were identified and classified into 4808 genes, including genes of Ͼ10 kbp. Of the 7067 genes obtained from the Y79 and ARPE-19 libraries, the authors selected 72 genes that were preferentially expressed in the eye, of which 131 clones corresponding to 57 genes were fully sequenced. As a result, we discovered many variants that were produced by different transcriptional start sites, alternative splicing, and alternative polyadenylation. CONCLUSIONS. The bias-free, full-length cDNA libraries constructed using the vector-capping method were shown to be useful for collecting an entire set of full-length cDNA clones for these retinal Cell Lines. Full-length transcriptome analysis of these cDNA libraries revealed that there were, unexpectedly, many transcript variants for each gene, indicating that obtaining the full-length cDNA for each variant is indispensable for analyzing its function. The full-length cDNA clones (approximately 80,000 clones each for ARPE-19 and Y79) will be useful as a resource for investigating the human retina. (Invest Ophthalmol Vis Sci. 2011;52:6662-6670
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full length transcriptome analysis of human retina derived Cell Lines arpe 19 and y79 using the vector capping method
Investigative Ophthalmology & Visual Science, 2011Co-Authors: Mio Oshikawa, Chihiro Tsutsui, Tomoko Ikegami, Yuki Fuchida, Maki Matsubara, Shigeru Toyama, Ron Usami, Kuniyo Ohtoko, Seishi KatoAbstract:PURPOSE. To collect an entire set of full-length cDNA clones derived from human retina-derived Cell Lines and to identify full-length transcripts for retinal preferentially expressed genes. METHODS. The full-length cDNA libraries were constructed from a Retinoblastoma Cell Line, Y79, and a retinal pigment epithelium Cell Line, ARPE-19, using the vector-capping method, which generates a genuine full-length cDNA. By single-pass sequencing of the 5'-end of cDNA clones and subsequent mapping to the human genome, the authors determined their transcriptional start sites and annotated the cDNA clones. RESULTS. Of the 23,616 clones isolated from Y79-derived cDNA libraries, 19,229 full-length cDNA clones were identified and classified into 4808 genes, including genes of >10 kbp. Of the 7067 genes obtained from the Y79 and ARPE-19 libraries, the authors selected 72 genes that were preferentially expressed in the eye, of which 131 clones corresponding to 57 genes were fully sequenced. As a result, we discovered many variants that were produced by different transcriptional start sites, alternative splicing, and alternative polyadenylation. CONCLUSIONS. The bias-free, full-length cDNA libraries constructed using the vector-capping method were shown to be useful for collecting an entire set of full-length cDNA clones for these retinal Cell Lines. Full-length transcriptome analysis of these cDNA libraries revealed that there were, unexpectedly, many transcript variants for each gene, indicating that obtaining the full-length cDNA for each variant is indispensable for analyzing its function. The full-length cDNA clones (approximately 80,000 clones each for ARPE-19 and Y79) will be useful as a resource for investigating the human retina.
Yuki Fuchida - One of the best experts on this subject based on the ideXlab platform.
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Full-Length Transcriptome Analysis of Human Retina-Derived Cell Lines ARPE-19 and Y79 Using the Vector-Capping Method
2020Co-Authors: Mio Oshikawa, Chihiro Tsutsui, Tomoko Ikegami, Yuki Fuchida, Maki Matsubara, Shigeru Toyama, Ron Usami, Kuniyo Ohtoko, Seishi KatoAbstract:PURPOSE. To collect an entire set of full-length cDNA clones derived from human retina-derived Cell Lines and to identify full-length transcripts for retinal preferentially expressed genes. METHODS. The full-length cDNA libraries were constructed from a Retinoblastoma Cell Line, Y79, and a retinal pigment epithelium Cell Line, ARPE-19, using the vector-capping method, which generates a genuine full-length cDNA. By single-pass sequencing of the 5Ј-end of cDNA clones and subsequent mapping to the human genome, the authors determined their transcriptional start sites and annotated the cDNA clones. RESULTS. Of the 23,616 clones isolated from Y79-derived cDNA libraries, 19,229 full-length cDNA clones were identified and classified into 4808 genes, including genes of Ͼ10 kbp. Of the 7067 genes obtained from the Y79 and ARPE-19 libraries, the authors selected 72 genes that were preferentially expressed in the eye, of which 131 clones corresponding to 57 genes were fully sequenced. As a result, we discovered many variants that were produced by different transcriptional start sites, alternative splicing, and alternative polyadenylation. CONCLUSIONS. The bias-free, full-length cDNA libraries constructed using the vector-capping method were shown to be useful for collecting an entire set of full-length cDNA clones for these retinal Cell Lines. Full-length transcriptome analysis of these cDNA libraries revealed that there were, unexpectedly, many transcript variants for each gene, indicating that obtaining the full-length cDNA for each variant is indispensable for analyzing its function. The full-length cDNA clones (approximately 80,000 clones each for ARPE-19 and Y79) will be useful as a resource for investigating the human retina. (Invest Ophthalmol Vis Sci. 2011;52:6662-6670
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full length transcriptome analysis of human retina derived Cell Lines arpe 19 and y79 using the vector capping method
Investigative Ophthalmology & Visual Science, 2011Co-Authors: Mio Oshikawa, Chihiro Tsutsui, Tomoko Ikegami, Yuki Fuchida, Maki Matsubara, Shigeru Toyama, Ron Usami, Kuniyo Ohtoko, Seishi KatoAbstract:PURPOSE. To collect an entire set of full-length cDNA clones derived from human retina-derived Cell Lines and to identify full-length transcripts for retinal preferentially expressed genes. METHODS. The full-length cDNA libraries were constructed from a Retinoblastoma Cell Line, Y79, and a retinal pigment epithelium Cell Line, ARPE-19, using the vector-capping method, which generates a genuine full-length cDNA. By single-pass sequencing of the 5'-end of cDNA clones and subsequent mapping to the human genome, the authors determined their transcriptional start sites and annotated the cDNA clones. RESULTS. Of the 23,616 clones isolated from Y79-derived cDNA libraries, 19,229 full-length cDNA clones were identified and classified into 4808 genes, including genes of >10 kbp. Of the 7067 genes obtained from the Y79 and ARPE-19 libraries, the authors selected 72 genes that were preferentially expressed in the eye, of which 131 clones corresponding to 57 genes were fully sequenced. As a result, we discovered many variants that were produced by different transcriptional start sites, alternative splicing, and alternative polyadenylation. CONCLUSIONS. The bias-free, full-length cDNA libraries constructed using the vector-capping method were shown to be useful for collecting an entire set of full-length cDNA clones for these retinal Cell Lines. Full-length transcriptome analysis of these cDNA libraries revealed that there were, unexpectedly, many transcript variants for each gene, indicating that obtaining the full-length cDNA for each variant is indispensable for analyzing its function. The full-length cDNA clones (approximately 80,000 clones each for ARPE-19 and Y79) will be useful as a resource for investigating the human retina.