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Fausto Foresti - One of the best experts on this subject based on the ideXlab platform.
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Illegal trade of the guitarfish Rhinobatos horkelii on the coasts of central and southern Brazil: genetic identification to aid conservation
Aquatic Conservation: Marine and Freshwater Ecosystems, 2012Co-Authors: Bruno Alexandre De‐franco, Fernando Fernandes Mendonça, Claudio Oliveira, Fausto ForestiAbstract:Among the diverse species of guitarfish, Rhinobatos horkelii is endemic to the south-west Atlantic and is primarily found off the Brazilian coast. The IUCN has classified this species as being in critical danger of extinction owing to widespread exploitation. Currently, this species is protected under Brazilian conservation laws. However, the morphological similarity of R. horkelii to other species precludes effective protection from fishing. Guitarfish samples were obtained from fishermen in different regions along the Brazilian coast and were identified using a genetic forensic method (multiplex-PCR). The analysis showed that 56% of the samples analysed were from R. horkelii, 25% from Rhinobatos percellens and 19% from Zapteryx brevirostris confirming that R. horkelii continues to be caught, despite conservation legislation. These results stress the need for effective conservation measures and may help to alert others to the occurrence of R. horkelii poaching. In addition, this work aims to establish an effective method of species identification to help prevent poaching of protected species such as R. horkelii. Copyright © 2012 John Wiley & Sons, Ltd.
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Forensic identification of the guitarfish species Rhinobatos horkelli, R. percellens and Zapteryx brevirostris using multiplex-PCR.
Molecular ecology resources, 2009Co-Authors: B. De-franco, Fernando Fernandes Mendonça, Diogo Teruo Hashimoto, Fábio Porto-foresti, Claudio Oliveira, Fausto ForestiAbstract:Rhinobatos percellens, Rhinobatos horkelli and Zapteryx brevirostris, known as guitarfish, are commonly found inshore and caught by trawl nets. Recently, these species have been suffering sharp population declines. One of the main impediments to the development of conservation plans is the lack of information related to each species catch. Gathering this information is made more difficult by the fishermen's practice of removing parts of the animals before landing, as well as the morphological similarities among the three species. To address these issues, this work presents multiplex-PCR protocols that were developed to distinguish among these species using their genetic characteristics.
Jordi Miquel - One of the best experts on this subject based on the ideXlab platform.
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Spermiogenesis and spermatozoon ultrastructure of the diphyllidean cestode Echinobothrium euterpes (Neifar, Tyler and Euzet 2001) Tyler 2006, a parasite of the common guitarfish Rhinobatos Rhinobatos
Parasitology research, 2011Co-Authors: Adji Mama Marigo, Catarina Eira, Jordi MiquelAbstract:Spermiogenesis and the ultrastructural characters of the spermatozoon of Echinobothrium euterpes are described by means of transmission electron microscopy, including cytochemical analysis for glycogen. Materials were obtained from a common guitarfish Rhinobatos Rhinobatos caught in the Gulf of Gabes (Tunisia). Spermiogenesis in E. euterpes is characterized by the orthogonal development of two unequal flagella followed by the flagellar rotation and the proximodistal fusion of these flagella with the median cytoplasmic process. The most interesting pattern characterizing the diphyllidean cestodes is the presence of a triangular body constituted by fines and dense granules without visible striation and assimilated at the striated rootlets. This pattern, only related in the Diphyllidea cestodes may be a synapomorphy of this order. Spermiogenesis is also characterized by the presence of a very short flagellum (around 1 μm long), observed in all the stages of spermiogenesis. This type of flagellum has never been commented in the diphyllidean cestodes and should be considered as an evolved character in this group. In the latest stage of spermiogenesis, this short axoneme probably degenerates. Thus, the mature spermatozoon of E. euterpes possesses only one axoneme of 9 + “1” trepaxonematan pattern. It also exhibits a single helical electron-dense crested body, a spiraled nucleus, few parallel cortical microtubules, and α-glycogen granules. Similitudes and differences between spermatozoa of diphyllideans are discussed.
B. De-franco - One of the best experts on this subject based on the ideXlab platform.
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MOLECULAR DIAGNOSTICS AND DNA TAXONOMY Forensic identification of the guitarfish species Rhinobatos horkelli,R. percellens and Zapteryx brevirostris using multiplex-PCR
2010Co-Authors: B. De-franco, F. P Orto-foresti, C. O Liveira, F. F Oresti, Estadual Paulista UnespAbstract:Abstract Rhinobatos percellens, Rhinobatos horkelli and Zapteryx brevirostris, known as guitarfish, arecommonly found inshore and caught by trawl ne ts. Recently, these species have been suffer-ing sharp population declines. One of the main impediments to the development of conser-vation plans is the lack of information related to each species catch. Gathering thisinformation is made more difficult by the fishermen’s practice of removing parts of theanimals before landing, as well as the morphological similarities among the three species. Toaddress these issues, this work presents multiplex-PCR protocols that were developed todistinguish among these species using their genetic characteristics.Keywords: commercial species, conservation, Cytochrome Oxidase I, Forensic genetics, raysReceived 1 April 2009; revision received 16 April 2009; accepted 17 April 2009The species Rhinobatos percellens (Walbaum, 1792) andRhinobatos horkelli (Muller & Henle, 1841), popularlyknown as guitarfish, have been highly exploited by fish-ing, particularly in the Brazilian continental shelf.According to capture records reported in scientificresearch papers, populations of rays of the genus Rhin-obatos have decreased by about 85% in the extreme southof Brazil between 1985 and 1997 (Miranda & Vooren2003). Despite the fact that these species, particularlyR. horkelli, undergo a serious risk of extinction, govern-ment measures to protect rays, such as an immediate banon their fishing and sale, have not yet been implemented.This may be because of a lack of official records of catchesof these species by the government regulatory agencies offishing activity. Unfortunately, one of the major impedi-ments in quantifying the captured fish of each species isthe inefficiency in the morphological identification ofindividuals. According to some studies, since 2001,reports of fishing of elasmobranches have begun tocontain numbers relating to guitarfish (FAO, 2006). Evenso, such classification does not distinguish between a spe-cies-specific level and groups of the species R. horkelli, R.percellens and Zapteryx brevirostris (Muller & Henle, 1841),which belong to the family Rhinobatidae, within a samefishing category.Because of the morphological similarities between thespecies of the family Rhinobatidae, coupled with thepractice of head removal just before disembarkation,morphological identification and recognition of particu-larities of each species becomes difficult.Species identification is important for managementand preservation plans. Thus, the development of practi-cal tools for the differentiation at the lowest taxonomiclevel has become essential. This work sought to developand propose a multiplex-PCR and protocol for the differ-entiation of Rhinobatos horkelli, R. percellens and Zapteryxbrevirostris species. This protocol may be applicable to theassessment of fishing exploitation, as well as to govern-mental planning and supervision.Samples were acquired from fishermen, who work inindustrial and artisanal fishing in several areas of theBrazilian coast. Among the samples of Rhinobatos
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Forensic identification of the guitarfish species Rhinobatos horkelli, R. percellens and Zapteryx brevirostris using multiplex-PCR.
Molecular ecology resources, 2009Co-Authors: B. De-franco, Fernando Fernandes Mendonça, Diogo Teruo Hashimoto, Fábio Porto-foresti, Claudio Oliveira, Fausto ForestiAbstract:Rhinobatos percellens, Rhinobatos horkelli and Zapteryx brevirostris, known as guitarfish, are commonly found inshore and caught by trawl nets. Recently, these species have been suffering sharp population declines. One of the main impediments to the development of conservation plans is the lack of information related to each species catch. Gathering this information is made more difficult by the fishermen's practice of removing parts of the animals before landing, as well as the morphological similarities among the three species. To address these issues, this work presents multiplex-PCR protocols that were developed to distinguish among these species using their genetic characteristics.
Fernando Fernandes Mendonça - One of the best experts on this subject based on the ideXlab platform.
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Illegal trade of the guitarfish Rhinobatos horkelii on the coasts of central and southern Brazil: genetic identification to aid conservation
Aquatic Conservation: Marine and Freshwater Ecosystems, 2012Co-Authors: Bruno Alexandre De‐franco, Fernando Fernandes Mendonça, Claudio Oliveira, Fausto ForestiAbstract:Among the diverse species of guitarfish, Rhinobatos horkelii is endemic to the south-west Atlantic and is primarily found off the Brazilian coast. The IUCN has classified this species as being in critical danger of extinction owing to widespread exploitation. Currently, this species is protected under Brazilian conservation laws. However, the morphological similarity of R. horkelii to other species precludes effective protection from fishing. Guitarfish samples were obtained from fishermen in different regions along the Brazilian coast and were identified using a genetic forensic method (multiplex-PCR). The analysis showed that 56% of the samples analysed were from R. horkelii, 25% from Rhinobatos percellens and 19% from Zapteryx brevirostris confirming that R. horkelii continues to be caught, despite conservation legislation. These results stress the need for effective conservation measures and may help to alert others to the occurrence of R. horkelii poaching. In addition, this work aims to establish an effective method of species identification to help prevent poaching of protected species such as R. horkelii. Copyright © 2012 John Wiley & Sons, Ltd.
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Forensic identification of the guitarfish species Rhinobatos horkelli, R. percellens and Zapteryx brevirostris using multiplex-PCR.
Molecular ecology resources, 2009Co-Authors: B. De-franco, Fernando Fernandes Mendonça, Diogo Teruo Hashimoto, Fábio Porto-foresti, Claudio Oliveira, Fausto ForestiAbstract:Rhinobatos percellens, Rhinobatos horkelli and Zapteryx brevirostris, known as guitarfish, are commonly found inshore and caught by trawl nets. Recently, these species have been suffering sharp population declines. One of the main impediments to the development of conservation plans is the lack of information related to each species catch. Gathering this information is made more difficult by the fishermen's practice of removing parts of the animals before landing, as well as the morphological similarities among the three species. To address these issues, this work presents multiplex-PCR protocols that were developed to distinguish among these species using their genetic characteristics.
Claudio Oliveira - One of the best experts on this subject based on the ideXlab platform.
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Illegal trade of the guitarfish Rhinobatos horkelii on the coasts of central and southern Brazil: genetic identification to aid conservation
Aquatic Conservation: Marine and Freshwater Ecosystems, 2012Co-Authors: Bruno Alexandre De‐franco, Fernando Fernandes Mendonça, Claudio Oliveira, Fausto ForestiAbstract:Among the diverse species of guitarfish, Rhinobatos horkelii is endemic to the south-west Atlantic and is primarily found off the Brazilian coast. The IUCN has classified this species as being in critical danger of extinction owing to widespread exploitation. Currently, this species is protected under Brazilian conservation laws. However, the morphological similarity of R. horkelii to other species precludes effective protection from fishing. Guitarfish samples were obtained from fishermen in different regions along the Brazilian coast and were identified using a genetic forensic method (multiplex-PCR). The analysis showed that 56% of the samples analysed were from R. horkelii, 25% from Rhinobatos percellens and 19% from Zapteryx brevirostris confirming that R. horkelii continues to be caught, despite conservation legislation. These results stress the need for effective conservation measures and may help to alert others to the occurrence of R. horkelii poaching. In addition, this work aims to establish an effective method of species identification to help prevent poaching of protected species such as R. horkelii. Copyright © 2012 John Wiley & Sons, Ltd.
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Forensic identification of the guitarfish species Rhinobatos horkelli, R. percellens and Zapteryx brevirostris using multiplex-PCR.
Molecular ecology resources, 2009Co-Authors: B. De-franco, Fernando Fernandes Mendonça, Diogo Teruo Hashimoto, Fábio Porto-foresti, Claudio Oliveira, Fausto ForestiAbstract:Rhinobatos percellens, Rhinobatos horkelli and Zapteryx brevirostris, known as guitarfish, are commonly found inshore and caught by trawl nets. Recently, these species have been suffering sharp population declines. One of the main impediments to the development of conservation plans is the lack of information related to each species catch. Gathering this information is made more difficult by the fishermen's practice of removing parts of the animals before landing, as well as the morphological similarities among the three species. To address these issues, this work presents multiplex-PCR protocols that were developed to distinguish among these species using their genetic characteristics.
