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Toshiaki Kudo - One of the best experts on this subject based on the ideXlab platform.
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three of the seven bphc genes of Rhodococcus erythropolis ta421 isolated from a termite ecosystem are located on an indigenous plasmid associated with biphenyl degradation
Applied and Environmental Microbiology, 1997Co-Authors: Saori Kosono, Michihisa Maeda, Fumie Fuji, Hiroyuki Arai, Toshiaki KudoAbstract:Rhodococcus erythropolis TA421, a polychlorinated biphenyl and biphenyl degrader isolated from a termite ecosystem, has seven bphC genes expressing 2,3-dihydroxybiphenyl dioxygenase activity. R. erythropolis TA421 harbored a large and probably linear plasmid on which three (bphC2, bphC3, and bphC4) of the seven bphC genes were located. A non-biphenyl-degrading mutant, designated strain TA422, was obtained spontaneously from R. erythropolis TA421. TA422 lacked the plasmid, suggesting that the three bphC genes were involved in the degradation of biphenyl. Southern blot analyses showed that R. erythropolis TA421 and Rhodococcus globerulus P6 have a similar set of bphC genes and that the genes for biphenyl catabolism are located on plasmids of different sizes. These results indicated that the genes encoding the biphenyl catabolic pathway in Rhodococcus strains are borne on plasmids.
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multiple genes encoding 2 3 dihydroxybiphenyl 1 2 dioxygenase in the gram positive polychlorinated biphenyl degrading bacterium Rhodococcus erythropolis ta421 isolated from a termite ecosystem
Applied and Environmental Microbiology, 1995Co-Authors: Michihisa Maeda, Seon-yong Chung, Eun Song, Toshiaki KudoAbstract:Rhodococcus erythropolis TA421 was isolated from a termite ecosystem and is able to degrade a wide range of polychlorinated biphenyl (PCB) congeners. Genetic and biochemical analyses of the PCB catabolic pathway of this organism revealed that there are four different bphC genes (bphC1, bphC2, bphC3, and bphC4) which encode 2,3-dihydroxybiphenyl dioxygenases. As determined by Southern hybridization, none of the bphC genes exhibits homology to any other bphC gene. bphC1, bphC2, and bphC4 encode enzymes that have narrow substrate specificities and cleave the first aromatic ring in the meta position. In contrast, bphC3 encodes a meta cleavage dioxygenase with broad substrate specificity. Asturias et al. have shown that the closely related organism Rhodococcus globerulus P6 contains three different bphC genes (bphC1, bphC2, and bpHC3) which encode meta cleavage dioxygenases. The data suggest that there is a diverse family of bphC genes which encode PCB meta cleavage dioxygenases in members of the genus Rhodococcus.
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a gram positive polychlorinated biphenyl degrading bacterium Rhodococcus erythropolis strain ta421 isolated from a termite ecosystem
Bioscience Biotechnology and Biochemistry, 1994Co-Authors: Seon-yong Chung, Michihisa Maeda, Eun Song, Koki Horikoshi, Toshiaki KudoAbstract:Gram-positive bacteria, identified as Rhodococcus erythropolis, were isolated from the ecosystem of the wood-feeding termite Reticulitermes speratus and found to aerobically degrade polychlorinated biphenyl (PCB) compounds. Rhodococcus erythropolis strain TA421 and strain TA431 were isolated by enrichment culture from termites obtained from different locations and each was found to be capable of degrading polychlorinated biphenyl (PCB) compounds to chlorobenzoates. These results suggest that the termite ecosystem is one possible habitat for biphenyl- and PCB-degrading Rhodococci. The spectrum of PCB-congeners degraded by strain TA421 is different from that of other, previously characterized PCB-degrading bacteria such as Rhodococcus globerulus strain P6 (formerly Corynebacterium sp. strain MB1) or Pseudomonas sp. strain LB400.
Michihisa Maeda - One of the best experts on this subject based on the ideXlab platform.
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three of the seven bphc genes of Rhodococcus erythropolis ta421 isolated from a termite ecosystem are located on an indigenous plasmid associated with biphenyl degradation
Applied and Environmental Microbiology, 1997Co-Authors: Saori Kosono, Michihisa Maeda, Fumie Fuji, Hiroyuki Arai, Toshiaki KudoAbstract:Rhodococcus erythropolis TA421, a polychlorinated biphenyl and biphenyl degrader isolated from a termite ecosystem, has seven bphC genes expressing 2,3-dihydroxybiphenyl dioxygenase activity. R. erythropolis TA421 harbored a large and probably linear plasmid on which three (bphC2, bphC3, and bphC4) of the seven bphC genes were located. A non-biphenyl-degrading mutant, designated strain TA422, was obtained spontaneously from R. erythropolis TA421. TA422 lacked the plasmid, suggesting that the three bphC genes were involved in the degradation of biphenyl. Southern blot analyses showed that R. erythropolis TA421 and Rhodococcus globerulus P6 have a similar set of bphC genes and that the genes for biphenyl catabolism are located on plasmids of different sizes. These results indicated that the genes encoding the biphenyl catabolic pathway in Rhodococcus strains are borne on plasmids.
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multiple genes encoding 2 3 dihydroxybiphenyl 1 2 dioxygenase in the gram positive polychlorinated biphenyl degrading bacterium Rhodococcus erythropolis ta421 isolated from a termite ecosystem
Applied and Environmental Microbiology, 1995Co-Authors: Michihisa Maeda, Seon-yong Chung, Eun Song, Toshiaki KudoAbstract:Rhodococcus erythropolis TA421 was isolated from a termite ecosystem and is able to degrade a wide range of polychlorinated biphenyl (PCB) congeners. Genetic and biochemical analyses of the PCB catabolic pathway of this organism revealed that there are four different bphC genes (bphC1, bphC2, bphC3, and bphC4) which encode 2,3-dihydroxybiphenyl dioxygenases. As determined by Southern hybridization, none of the bphC genes exhibits homology to any other bphC gene. bphC1, bphC2, and bphC4 encode enzymes that have narrow substrate specificities and cleave the first aromatic ring in the meta position. In contrast, bphC3 encodes a meta cleavage dioxygenase with broad substrate specificity. Asturias et al. have shown that the closely related organism Rhodococcus globerulus P6 contains three different bphC genes (bphC1, bphC2, and bpHC3) which encode meta cleavage dioxygenases. The data suggest that there is a diverse family of bphC genes which encode PCB meta cleavage dioxygenases in members of the genus Rhodococcus.
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a gram positive polychlorinated biphenyl degrading bacterium Rhodococcus erythropolis strain ta421 isolated from a termite ecosystem
Bioscience Biotechnology and Biochemistry, 1994Co-Authors: Seon-yong Chung, Michihisa Maeda, Eun Song, Koki Horikoshi, Toshiaki KudoAbstract:Gram-positive bacteria, identified as Rhodococcus erythropolis, were isolated from the ecosystem of the wood-feeding termite Reticulitermes speratus and found to aerobically degrade polychlorinated biphenyl (PCB) compounds. Rhodococcus erythropolis strain TA421 and strain TA431 were isolated by enrichment culture from termites obtained from different locations and each was found to be capable of degrading polychlorinated biphenyl (PCB) compounds to chlorobenzoates. These results suggest that the termite ecosystem is one possible habitat for biphenyl- and PCB-degrading Rhodococci. The spectrum of PCB-congeners degraded by strain TA421 is different from that of other, previously characterized PCB-degrading bacteria such as Rhodococcus globerulus strain P6 (formerly Corynebacterium sp. strain MB1) or Pseudomonas sp. strain LB400.
Skaliczki Gábor - One of the best experts on this subject based on the ideXlab platform.
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Rhodococcus equi által okozott nagyízületi endoprotézis körül kialakult fertőzés | Periprosthetic joint infection caused by Rhodococcus equi. Case report
'Akademiai Kiado Zrt.', 2017Co-Authors: Sallai Imre, Péterfy Nóra, Sanatkhani Mohammad, Bejek Zoltán, Antal Imre, Prinz Gyula, Kristóf Katalin, Skaliczki GáborAbstract:Absztrakt: A Rhodococcus equi ritka patogén, amely főként gyengült immunrendszerű vagy immunszupprimált betegekben okoz fertőzést. Esetbemutatásunkban Rhodococcus equi által okozott nagyízületi endoprotézis körül kialakult fertőzést mutatunk be, amelynek leírására még nem volt példa a nemzetközi irodalomban. A 88 éves férfi beteget láz és jobb oldali csípőfájdalom miatt vettük fel klinikánkra. A beteg anamnézisében több, mindkét oldalt érintő csípőműtét szerepelt, és a jobb oldalon aszeptikus lazulás miatt korábban revíziós arthroplastica is történt. Ismert betegségei között szerepelt cukorbetegség, diabeteses nephropathia, valamint korábbi stroke miatt bal oldali hemiplegia, azonban kifejezett immunszuppresszió nem. A képalkotó vizsgálatok a jobb oldali csípőprotézis komponenseit stabilnak mutatták, az ízületi punkcióból pedig Rhodococcus equi tenyészett ki. A betegnek azonnali feltárást és a protézis körüli terület tisztítását javasoltuk, amit visszautasított. Konzervatív kezelést, parenteralis antibiotikum-terápiát kezdtünk. A beteg gyulladásos paraméterei csökkentek, fájdalma megszűnt. Esettanulmányunk célja, hogy a nagyízületi protézisek szeptikus komplikációi kapcsán felhívjuk a figyelmet a ritka patogének növekvő szerepére. Orv Hetil. 2017; 158(27): 1071–1074. | Abstract: Rhodococcus equi is a rare pathogen in humans causing infections mostly in immunocompromised hosts. We present the first case of periprosthetic joint infection caused by Rhodococcus equi. An 88-year-old male patient was referred to our clinic with a history of fever and right hip pain. The patient had multiple hip surgeries including total joint arthroplasty and revision for aseptic loosening on the right side. He was immunocompetent, but his additional medical history was remarkable for diabetes mellitus, diabetic nephropathy and stroke with hemiplegia resulting in immobilization. Radiography showed stable components, joint aspirate yielded Rhodococcus equi. Irrigation and debridement was proposed, but the patient refused any surgical intervention. Therefore antibiotic therapy was administered. At the last follow-up the patient is free of complaints but the C-reactive protein level is still elevated. This case illustrates the possible role of Rhodococcus equi in medical device-associated infections. Orv Hetil. 2017; 158(27): 1071–1074
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Rhodococcus equi által okozott nagyízületi endoprotézis körül kialakult fertőzés
2017Co-Authors: Sallai Imre, Péterfy Nóra, Bejek Zoltán, Antal Imre, Prinz Gyula, Kristóf Katalin, Mohammad Sanatkhani, Skaliczki GáborAbstract:Rhodococcus equi is a rare pathogen in humans causing infections mostly in immunocompromised hosts. We present the first case of periprosthetic joint infection caused by Rhodococcus equi. An 88-year-old male patient was referred to our clinic with a history of fever and right hip pain. The patient had multiple hip surgeries including total joint arthroplasty and revision for aseptic loosening on the right side. He was immunocompetent, but his additional medical history was remarkable for diabetes mellitus, diabetic nephropathy and stroke with hemiplegia resulting in immobilization. Radiography showed stable components, joint aspirate yielded Rhodococcus equi. Irrigation and debridement was proposed, but the patient refused any surgical intervention. Therefore antibiotic therapy was administered. At the last follow-up the patient is free of complaints but the C-reactive protein level is still elevated. This case illustrates the possible role of Rhodococcus equi in medical device-associated infections. Orv Hetil. 2017; 158(27): 1071-1074
George M Garrity - One of the best experts on this subject based on the ideXlab platform.
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conservation of Rhodococcus equi magnusson 1923 goodfellow and alderson 1977 and rejection of corynebacterium hoagii morse 1912 eberson 1918
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: George M GarrityAbstract:A recent review of the nomenclatural history of Rhodococcus equi and its heterotypic synonyms reveals a situation in which the strict application of the Rules of the International Code of Nomenclature of Prokaryotes have resulted in the renaming of this known zoonotic pathogen, which may be reasonably viewed as a perilous name. This situation can be remedied only by the Judicial Commission rendering an opinion to conserve the name Rhodococcus equi and to reject its earlier heterotypic synonym, Corynebacterium hoagii .
Seon-yong Chung - One of the best experts on this subject based on the ideXlab platform.
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Phylogenetic analysis of long-chain hydrocarbon-degrading bacteria and evaluation of their hydrocarbon-degradation by the 2,6-DCPIP assay.
Biodegradation, 2008Co-Authors: Kenzo Kubota, Daisuke Koma, Yoshiki Matsumiya, Seon-yong Chung, Motoki KuboAbstract:Thirty-six bacteria that degraded long-chain hydrocarbons were isolated from natural environments using long-chain hydrocarbons (waste car engine oil, base oil or the c-alkane fraction of base oil) as the sole carbon and energy source. A phylogenetic tree of the isolates constructed using their 16S rDNA sequences revealed that the isolates were divided into six genera plus one family (Acinetobacter, Rhodococcus, Gordonia, Pseudomonas, Ralstonia, Bacillus and Alcaligenaceae, respectively). Furthermore, most of the isolates (27 of 36) were classified into the genera Acinetobacter, Rhodococcus or Gordonia. The hydrocarbon-degradation similarity in each strain was confirmed by the 2,6-dichlorophenol indophenol (2,6-DCPIP) assay. Isolates belonging to the genus Acinetobacter degraded long-chain normal alkanes (n-alkanes) but did not degrade short-chain n-alkanes or cyclic alkanes (c-alkanes), while isolates belonging to the genera Rhodococcus and Gordonia degraded both long-chain n-alkanes and c-alkanes.
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multiple genes encoding 2 3 dihydroxybiphenyl 1 2 dioxygenase in the gram positive polychlorinated biphenyl degrading bacterium Rhodococcus erythropolis ta421 isolated from a termite ecosystem
Applied and Environmental Microbiology, 1995Co-Authors: Michihisa Maeda, Seon-yong Chung, Eun Song, Toshiaki KudoAbstract:Rhodococcus erythropolis TA421 was isolated from a termite ecosystem and is able to degrade a wide range of polychlorinated biphenyl (PCB) congeners. Genetic and biochemical analyses of the PCB catabolic pathway of this organism revealed that there are four different bphC genes (bphC1, bphC2, bphC3, and bphC4) which encode 2,3-dihydroxybiphenyl dioxygenases. As determined by Southern hybridization, none of the bphC genes exhibits homology to any other bphC gene. bphC1, bphC2, and bphC4 encode enzymes that have narrow substrate specificities and cleave the first aromatic ring in the meta position. In contrast, bphC3 encodes a meta cleavage dioxygenase with broad substrate specificity. Asturias et al. have shown that the closely related organism Rhodococcus globerulus P6 contains three different bphC genes (bphC1, bphC2, and bpHC3) which encode meta cleavage dioxygenases. The data suggest that there is a diverse family of bphC genes which encode PCB meta cleavage dioxygenases in members of the genus Rhodococcus.
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a gram positive polychlorinated biphenyl degrading bacterium Rhodococcus erythropolis strain ta421 isolated from a termite ecosystem
Bioscience Biotechnology and Biochemistry, 1994Co-Authors: Seon-yong Chung, Michihisa Maeda, Eun Song, Koki Horikoshi, Toshiaki KudoAbstract:Gram-positive bacteria, identified as Rhodococcus erythropolis, were isolated from the ecosystem of the wood-feeding termite Reticulitermes speratus and found to aerobically degrade polychlorinated biphenyl (PCB) compounds. Rhodococcus erythropolis strain TA421 and strain TA431 were isolated by enrichment culture from termites obtained from different locations and each was found to be capable of degrading polychlorinated biphenyl (PCB) compounds to chlorobenzoates. These results suggest that the termite ecosystem is one possible habitat for biphenyl- and PCB-degrading Rhodococci. The spectrum of PCB-congeners degraded by strain TA421 is different from that of other, previously characterized PCB-degrading bacteria such as Rhodococcus globerulus strain P6 (formerly Corynebacterium sp. strain MB1) or Pseudomonas sp. strain LB400.
