The Experts below are selected from a list of 252 Experts worldwide ranked by ideXlab platform
R V Gadre - One of the best experts on this subject based on the ideXlab platform.
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optimization of carotenoid production from hyper producing Rhodotorula glutinis mutant 32 by a factorial approach
Letters in Applied Microbiology, 2001Co-Authors: P Bhosale, R V GadreAbstract:Aims: Optimization of carotenoid production by a mutant of Rhodotorula glutinis. Methods: The growth and carotenoid production was optimized in shake flasks using a two-level, three-variable factorial design. Results: The volumetric carotenoid production could be increased to 129 ± 2 mg 1−1 in a medium containing (g l−1) yeast extract 11·74, glucose 46 and threonine 18 along with other micronutrients, wherein, β-carotene yield was 102 ± 2 mg l−1, accounting for 80% of the total carotenoids. Significance and Impact of the Study: The medium optimization resulted in a fourfold increase in volumetric production and a twofold increase in the cellular accumulation of carotenoids. In view of such high yields, the mutant of Rhodotorula glutinis can be a potential source of β-carotene.
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Optimization of carotenoid production from hyper‐producing Rhodotorula glutinis mutant 32 by a factorial approach
Letters in Applied Microbiology, 2001Co-Authors: P Bhosale, R V GadreAbstract:Aims: Optimization of carotenoid production by a mutant of Rhodotorula glutinis. Methods: The growth and carotenoid production was optimized in shake flasks using a two-level, three-variable factorial design. Results: The volumetric carotenoid production could be increased to 129 ± 2 mg 1−1 in a medium containing (g l−1) yeast extract 11·74, glucose 46 and threonine 18 along with other micronutrients, wherein, β-carotene yield was 102 ± 2 mg l−1, accounting for 80% of the total carotenoids. Significance and Impact of the Study: The medium optimization resulted in a fourfold increase in volumetric production and a twofold increase in the cellular accumulation of carotenoids. In view of such high yields, the mutant of Rhodotorula glutinis can be a potential source of β-carotene.
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production of β carotene by a Rhodotorula glutinis mutant in sea water medium
Bioresource Technology, 2001Co-Authors: Prakash Bhosale, R V GadreAbstract:Abstract Mutant 32, derived from Rhodotorula glutinis NCIM 3353 produced 76-fold more β-carotene than the parent strain. In the growth medium prepared in seawater, the total carotenoid content and dry cell mass was 86 mg/l and 16 g/l, respectively, as compared to 70 mg/l and 12 g/l obtained with a medium prepared in distilled water. A 2-fold increase in β-carotene with simultaneous 2.3-fold decrease in torulene content was also observed. When grown in seawater medium at pH 6.0, 83±5% carotenoids could be extracted from the cells without any mechanical disintegration.
V. N. Ziogas - One of the best experts on this subject based on the ideXlab platform.
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Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra
Ecotoxicology, 2018Co-Authors: E. D. Bempelou, J. G. Vontas, K. S. Liapis, V. N. ZiogasAbstract:Biodegradation of chlorpyrifos by Rhodotorula glutinis and Rhodotorula rubra Biodegradation of TCP by Rhodotorula glutinis and Rhodotorula rubra Triphenyl phosphate inhibited the biodegradation of chlorpyrifos The possible involvement of the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra in the biodegradation of the insecticide chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyridinol (TCP), in pure cultures and in plant surfaces (tomato fruits) was investigated. Higher biodegradation rates were observed as the concentration of chlorpyrifos and the inoculum of the microorganisms were increased, while the yeasts proved to be more active at 25 and 15 °C. The presence of glucose in the mineral nutrient medium, as an extra source of carbon, delayed the biodegradation by Rhodotorula glutinis , while Rhodotorula rubra proved to be more active. The detection and quantification of the parent compound and TCP was successfully achieved using a LC/MS/MS chromatographic system. The in vitro enzymatic assays applied suggested that esterases may be involved in the biodegradation of chlorpyrifos, a fact that was further enhanced after the addition of the synergists triphenyl phosphate, diethyl maleate and piperonyl butoxide in the biodegradation trials. The decrease of chlorpyrifos residues on tomato fruits confirmed the corresponding on pure cultures, resulting in the suggestion that the yeasts R. glutinis and R. rubra can possibly be used successfully for the removal or detoxification of chlorpyrifos residues on tomatoes.
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biodegradation of chlorpyrifos and 3 5 6 trichloro 2 pyridinol by the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra
Ecotoxicology, 2018Co-Authors: E. D. Bempelou, J. G. Vontas, K. S. Liapis, V. N. ZiogasAbstract:The possible involvement of the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra in the biodegradation of the insecticide chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyridinol (TCP), in pure cultures and in plant surfaces (tomato fruits) was investigated. Higher biodegradation rates were observed as the concentration of chlorpyrifos and the inoculum of the microorganisms were increased, while the yeasts proved to be more active at 25 and 15 °C. The presence of glucose in the mineral nutrient medium, as an extra source of carbon, delayed the biodegradation by Rhodotorula glutinis, while Rhodotorula rubra proved to be more active. The detection and quantification of the parent compound and TCP was successfully achieved using a LC/MS/MS chromatographic system. The in vitro enzymatic assays applied suggested that esterases may be involved in the biodegradation of chlorpyrifos, a fact that was further enhanced after the addition of the synergists triphenyl phosphate, diethyl maleate and piperonyl butoxide in the biodegradation trials. The decrease of chlorpyrifos residues on tomato fruits confirmed the corresponding on pure cultures, resulting in the suggestion that the yeasts R. glutinis and R. rubra can possibly be used successfully for the removal or detoxification of chlorpyrifos residues on tomatoes.
E. D. Bempelou - One of the best experts on this subject based on the ideXlab platform.
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Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra
Ecotoxicology, 2018Co-Authors: E. D. Bempelou, J. G. Vontas, K. S. Liapis, V. N. ZiogasAbstract:Biodegradation of chlorpyrifos by Rhodotorula glutinis and Rhodotorula rubra Biodegradation of TCP by Rhodotorula glutinis and Rhodotorula rubra Triphenyl phosphate inhibited the biodegradation of chlorpyrifos The possible involvement of the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra in the biodegradation of the insecticide chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyridinol (TCP), in pure cultures and in plant surfaces (tomato fruits) was investigated. Higher biodegradation rates were observed as the concentration of chlorpyrifos and the inoculum of the microorganisms were increased, while the yeasts proved to be more active at 25 and 15 °C. The presence of glucose in the mineral nutrient medium, as an extra source of carbon, delayed the biodegradation by Rhodotorula glutinis , while Rhodotorula rubra proved to be more active. The detection and quantification of the parent compound and TCP was successfully achieved using a LC/MS/MS chromatographic system. The in vitro enzymatic assays applied suggested that esterases may be involved in the biodegradation of chlorpyrifos, a fact that was further enhanced after the addition of the synergists triphenyl phosphate, diethyl maleate and piperonyl butoxide in the biodegradation trials. The decrease of chlorpyrifos residues on tomato fruits confirmed the corresponding on pure cultures, resulting in the suggestion that the yeasts R. glutinis and R. rubra can possibly be used successfully for the removal or detoxification of chlorpyrifos residues on tomatoes.
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biodegradation of chlorpyrifos and 3 5 6 trichloro 2 pyridinol by the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra
Ecotoxicology, 2018Co-Authors: E. D. Bempelou, J. G. Vontas, K. S. Liapis, V. N. ZiogasAbstract:The possible involvement of the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra in the biodegradation of the insecticide chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyridinol (TCP), in pure cultures and in plant surfaces (tomato fruits) was investigated. Higher biodegradation rates were observed as the concentration of chlorpyrifos and the inoculum of the microorganisms were increased, while the yeasts proved to be more active at 25 and 15 °C. The presence of glucose in the mineral nutrient medium, as an extra source of carbon, delayed the biodegradation by Rhodotorula glutinis, while Rhodotorula rubra proved to be more active. The detection and quantification of the parent compound and TCP was successfully achieved using a LC/MS/MS chromatographic system. The in vitro enzymatic assays applied suggested that esterases may be involved in the biodegradation of chlorpyrifos, a fact that was further enhanced after the addition of the synergists triphenyl phosphate, diethyl maleate and piperonyl butoxide in the biodegradation trials. The decrease of chlorpyrifos residues on tomato fruits confirmed the corresponding on pure cultures, resulting in the suggestion that the yeasts R. glutinis and R. rubra can possibly be used successfully for the removal or detoxification of chlorpyrifos residues on tomatoes.
J. G. Vontas - One of the best experts on this subject based on the ideXlab platform.
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Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra
Ecotoxicology, 2018Co-Authors: E. D. Bempelou, J. G. Vontas, K. S. Liapis, V. N. ZiogasAbstract:Biodegradation of chlorpyrifos by Rhodotorula glutinis and Rhodotorula rubra Biodegradation of TCP by Rhodotorula glutinis and Rhodotorula rubra Triphenyl phosphate inhibited the biodegradation of chlorpyrifos The possible involvement of the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra in the biodegradation of the insecticide chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyridinol (TCP), in pure cultures and in plant surfaces (tomato fruits) was investigated. Higher biodegradation rates were observed as the concentration of chlorpyrifos and the inoculum of the microorganisms were increased, while the yeasts proved to be more active at 25 and 15 °C. The presence of glucose in the mineral nutrient medium, as an extra source of carbon, delayed the biodegradation by Rhodotorula glutinis , while Rhodotorula rubra proved to be more active. The detection and quantification of the parent compound and TCP was successfully achieved using a LC/MS/MS chromatographic system. The in vitro enzymatic assays applied suggested that esterases may be involved in the biodegradation of chlorpyrifos, a fact that was further enhanced after the addition of the synergists triphenyl phosphate, diethyl maleate and piperonyl butoxide in the biodegradation trials. The decrease of chlorpyrifos residues on tomato fruits confirmed the corresponding on pure cultures, resulting in the suggestion that the yeasts R. glutinis and R. rubra can possibly be used successfully for the removal or detoxification of chlorpyrifos residues on tomatoes.
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biodegradation of chlorpyrifos and 3 5 6 trichloro 2 pyridinol by the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra
Ecotoxicology, 2018Co-Authors: E. D. Bempelou, J. G. Vontas, K. S. Liapis, V. N. ZiogasAbstract:The possible involvement of the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra in the biodegradation of the insecticide chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyridinol (TCP), in pure cultures and in plant surfaces (tomato fruits) was investigated. Higher biodegradation rates were observed as the concentration of chlorpyrifos and the inoculum of the microorganisms were increased, while the yeasts proved to be more active at 25 and 15 °C. The presence of glucose in the mineral nutrient medium, as an extra source of carbon, delayed the biodegradation by Rhodotorula glutinis, while Rhodotorula rubra proved to be more active. The detection and quantification of the parent compound and TCP was successfully achieved using a LC/MS/MS chromatographic system. The in vitro enzymatic assays applied suggested that esterases may be involved in the biodegradation of chlorpyrifos, a fact that was further enhanced after the addition of the synergists triphenyl phosphate, diethyl maleate and piperonyl butoxide in the biodegradation trials. The decrease of chlorpyrifos residues on tomato fruits confirmed the corresponding on pure cultures, resulting in the suggestion that the yeasts R. glutinis and R. rubra can possibly be used successfully for the removal or detoxification of chlorpyrifos residues on tomatoes.
K. S. Liapis - One of the best experts on this subject based on the ideXlab platform.
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Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra
Ecotoxicology, 2018Co-Authors: E. D. Bempelou, J. G. Vontas, K. S. Liapis, V. N. ZiogasAbstract:Biodegradation of chlorpyrifos by Rhodotorula glutinis and Rhodotorula rubra Biodegradation of TCP by Rhodotorula glutinis and Rhodotorula rubra Triphenyl phosphate inhibited the biodegradation of chlorpyrifos The possible involvement of the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra in the biodegradation of the insecticide chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyridinol (TCP), in pure cultures and in plant surfaces (tomato fruits) was investigated. Higher biodegradation rates were observed as the concentration of chlorpyrifos and the inoculum of the microorganisms were increased, while the yeasts proved to be more active at 25 and 15 °C. The presence of glucose in the mineral nutrient medium, as an extra source of carbon, delayed the biodegradation by Rhodotorula glutinis , while Rhodotorula rubra proved to be more active. The detection and quantification of the parent compound and TCP was successfully achieved using a LC/MS/MS chromatographic system. The in vitro enzymatic assays applied suggested that esterases may be involved in the biodegradation of chlorpyrifos, a fact that was further enhanced after the addition of the synergists triphenyl phosphate, diethyl maleate and piperonyl butoxide in the biodegradation trials. The decrease of chlorpyrifos residues on tomato fruits confirmed the corresponding on pure cultures, resulting in the suggestion that the yeasts R. glutinis and R. rubra can possibly be used successfully for the removal or detoxification of chlorpyrifos residues on tomatoes.
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biodegradation of chlorpyrifos and 3 5 6 trichloro 2 pyridinol by the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra
Ecotoxicology, 2018Co-Authors: E. D. Bempelou, J. G. Vontas, K. S. Liapis, V. N. ZiogasAbstract:The possible involvement of the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra in the biodegradation of the insecticide chlorpyrifos and its metabolite 3,5,6-trichloro-2-pyridinol (TCP), in pure cultures and in plant surfaces (tomato fruits) was investigated. Higher biodegradation rates were observed as the concentration of chlorpyrifos and the inoculum of the microorganisms were increased, while the yeasts proved to be more active at 25 and 15 °C. The presence of glucose in the mineral nutrient medium, as an extra source of carbon, delayed the biodegradation by Rhodotorula glutinis, while Rhodotorula rubra proved to be more active. The detection and quantification of the parent compound and TCP was successfully achieved using a LC/MS/MS chromatographic system. The in vitro enzymatic assays applied suggested that esterases may be involved in the biodegradation of chlorpyrifos, a fact that was further enhanced after the addition of the synergists triphenyl phosphate, diethyl maleate and piperonyl butoxide in the biodegradation trials. The decrease of chlorpyrifos residues on tomato fruits confirmed the corresponding on pure cultures, resulting in the suggestion that the yeasts R. glutinis and R. rubra can possibly be used successfully for the removal or detoxification of chlorpyrifos residues on tomatoes.