The Experts below are selected from a list of 2013 Experts worldwide ranked by ideXlab platform
Lucia I C De Figueroa - One of the best experts on this subject based on the ideXlab platform.
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relationship among carotenoid production copper bioremediation and oxidative stress in Rhodotorula mucilaginosa rcl 11
Process Biochemistry, 2013Co-Authors: Veronica Irazusta, Carlos G Nietopenalver, Maria Eugenia Sesto Cabral, Maria Julia Del R Amoroso, Lucia I C De FigueroaAbstract:Rhodotorula mucilaginosa RCL-11, a pigmented yeast isolated from a filter plant of a copper mine in the province of Tucuman, Argentina, supports high concentrations of the heavy metal Cu(II). Copper overload augmented carotenoid biosynthesis in this yeast, modifying at the same time the relative proportion of the pigments produced. Inhibition of the synthesis pathway with diphenylamine suggests an inverse relationship between carotenoid and copper biosorption by R. mucilaginosa RCL-11. The increased activity of superoxide dismutase and catalase measured under inhibition of carotenoid biosynthesis could explain these observations. Exposure to H2O2, a second oxidative stress agent, alone or in combination with Cu(II) also modified the carotenoid content, both qualitatively and quantitatively. The change in the relative proportion of the carotenoids torularhodin, torulene and beta-carotena, as well as the detection of gamma-carotene in the presence of H2O2 and Cu(II) allows to hypothesize that the carotenoids produced by R. mucilaginosa RCL-11 plays different roles in the oxidative stress response of this yeast.
Zhenming Chi - One of the best experts on this subject based on the ideXlab platform.
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direct conversion of inulin and extract of tubers of jerusalem artichoke into single cell oil by co cultures of Rhodotorula mucilaginosa tjy15a and immobilized inulinase producing yeast cells
Bioresource Technology, 2011Co-Authors: Chunhai Zhao, Fang Zhang, Zhe Chi, Fengjun Guo, Weibo Song, Zhenming ChiAbstract:Abstract In this study, it was found that the immobilized inulinase-producing cells of Pichia guilliermondii M-30 could produce 169.3 U/ml of inulinase activity while the free cells of the same yeast strain only produced 124.3 U/ml of inulinase activity within 48 h. When the immobilized inulinase-producing yeast cells were co-cultivated with the free cells of Rhodotorula mucilaginosa TJY15a, R. mucilaginosa TJY15a could accumulate 53.2% oil from inulin in its cells and cell dry weight reached 12.2 g/l. Under the similar conditions, R. mucilaginosa TJY15a could accumulate 55.4% (w/w) oil from the extract of Jerusalem artichoke tubers in its cells and cell dry weight reached 12.8 g/l within 48 h. When the co-cultures were grown in 2 l fermentor, R. mucilaginosa TJY15a could accumulate 56.6% (w/w) oil from the extract of Jerusalem artichoke tubers in its cells and cell dry weight reached 19.6 g/l within 48 h. Over 90.0% of the fatty acids from the yeast strain TJY15a grown in the extract of Jerusalem artichoke tubers was C 16:0 , C 18:1 and C 18:2 , especially C 18:1 (50.6%).
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single cell oil production from hydrolysates of inulin and extract of tubers of jerusalem artichoke by Rhodotorula mucilaginosa tjy15a
Process Biochemistry, 2010Co-Authors: Chunhai Zhao, Tong Zhang, Zhenming ChiAbstract:Abstract In this study, we found that Rhodotorula mucilaginosa TJY15a could accumulate 48.8% (w/w) oil from hydrolysate of inulin and its cell dry weight reached 14.8 g/l during the batch cultivation while it could accumulate 48.6% (w/w) oil and 52.2% (w/w) oil from hydrolysate of extract of Jerusalem artichoke tubers and its cell dry weight reached 14.4 g/l and 19.5 g/l during the batch and fed-batch cultivations, respectively. At the end of the fed-batch cultivation, only 0.04% of reducing sugar and 0.08% of total sugar were left in the fermented medium. Over 87.6% of the fatty acids from the yeast strain TJY15a cultivated in the hydrolysate of extract of Jerusalem artichoke tubers was C 16:0 , C 18:1 and C 18:2 , especially C 18:1 (54.7%). Therefore, the results show that hydrolysates of inulin and extract of Jerusalem artichoke tubers were also the good materials for single cell oil production.
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single cell oil production from hydrolysate of cassava starch by marine derived yeast Rhodotorula mucilaginosa tjy15a
Biomass & Bioenergy, 2010Co-Authors: Guanglei Liu, Zhe Chi, Zhenming ChiAbstract:Abstract Rhodotorula mucilaginosa TJY15a which was isolated from surface of marine fish could accumulate a large amount of lipid from hydrolysate of cassava starch. The cells contained 47.9% (w/w) oil during batch cultivation, whereas 52.9% (w/w) of lipid was obtained during the fed-batch cultivation. At the end of the fed-batch cultivation, all the starch were converted into reducing sugar and only 0.34 g dm −3 of reducing sugar was left in the fermented medium. Therefore, the marine-derived R. mucilaginosa TJY15a was another candidate for single cell oil production. The fatty acids from R. mucilaginosa TJY15a were mainly composed of palmitic acid (C 16:0 ), palmitoleic acid (C 16:1 ), stearic acid (C 18:0 ), oleic acid (C 18:1 ) and linolenic acid (C 18:2 ), suggesting that the fatty acids could be used as feedstock for biodiesel production.
Maria Julia Del R Amoroso - One of the best experts on this subject based on the ideXlab platform.
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relationship among carotenoid production copper bioremediation and oxidative stress in Rhodotorula mucilaginosa rcl 11
Process Biochemistry, 2013Co-Authors: Veronica Irazusta, Carlos G Nietopenalver, Maria Eugenia Sesto Cabral, Maria Julia Del R Amoroso, Lucia I C De FigueroaAbstract:Rhodotorula mucilaginosa RCL-11, a pigmented yeast isolated from a filter plant of a copper mine in the province of Tucuman, Argentina, supports high concentrations of the heavy metal Cu(II). Copper overload augmented carotenoid biosynthesis in this yeast, modifying at the same time the relative proportion of the pigments produced. Inhibition of the synthesis pathway with diphenylamine suggests an inverse relationship between carotenoid and copper biosorption by R. mucilaginosa RCL-11. The increased activity of superoxide dismutase and catalase measured under inhibition of carotenoid biosynthesis could explain these observations. Exposure to H2O2, a second oxidative stress agent, alone or in combination with Cu(II) also modified the carotenoid content, both qualitatively and quantitatively. The change in the relative proportion of the carotenoids torularhodin, torulene and beta-carotena, as well as the detection of gamma-carotene in the presence of H2O2 and Cu(II) allows to hypothesize that the carotenoids produced by R. mucilaginosa RCL-11 plays different roles in the oxidative stress response of this yeast.
Carlos G Nietopenalver - One of the best experts on this subject based on the ideXlab platform.
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relationship among carotenoid production copper bioremediation and oxidative stress in Rhodotorula mucilaginosa rcl 11
Process Biochemistry, 2013Co-Authors: Veronica Irazusta, Carlos G Nietopenalver, Maria Eugenia Sesto Cabral, Maria Julia Del R Amoroso, Lucia I C De FigueroaAbstract:Rhodotorula mucilaginosa RCL-11, a pigmented yeast isolated from a filter plant of a copper mine in the province of Tucuman, Argentina, supports high concentrations of the heavy metal Cu(II). Copper overload augmented carotenoid biosynthesis in this yeast, modifying at the same time the relative proportion of the pigments produced. Inhibition of the synthesis pathway with diphenylamine suggests an inverse relationship between carotenoid and copper biosorption by R. mucilaginosa RCL-11. The increased activity of superoxide dismutase and catalase measured under inhibition of carotenoid biosynthesis could explain these observations. Exposure to H2O2, a second oxidative stress agent, alone or in combination with Cu(II) also modified the carotenoid content, both qualitatively and quantitatively. The change in the relative proportion of the carotenoids torularhodin, torulene and beta-carotena, as well as the detection of gamma-carotene in the presence of H2O2 and Cu(II) allows to hypothesize that the carotenoids produced by R. mucilaginosa RCL-11 plays different roles in the oxidative stress response of this yeast.
Veronica Irazusta - One of the best experts on this subject based on the ideXlab platform.
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relationship among carotenoid production copper bioremediation and oxidative stress in Rhodotorula mucilaginosa rcl 11
Process Biochemistry, 2013Co-Authors: Veronica Irazusta, Carlos G Nietopenalver, Maria Eugenia Sesto Cabral, Maria Julia Del R Amoroso, Lucia I C De FigueroaAbstract:Rhodotorula mucilaginosa RCL-11, a pigmented yeast isolated from a filter plant of a copper mine in the province of Tucuman, Argentina, supports high concentrations of the heavy metal Cu(II). Copper overload augmented carotenoid biosynthesis in this yeast, modifying at the same time the relative proportion of the pigments produced. Inhibition of the synthesis pathway with diphenylamine suggests an inverse relationship between carotenoid and copper biosorption by R. mucilaginosa RCL-11. The increased activity of superoxide dismutase and catalase measured under inhibition of carotenoid biosynthesis could explain these observations. Exposure to H2O2, a second oxidative stress agent, alone or in combination with Cu(II) also modified the carotenoid content, both qualitatively and quantitatively. The change in the relative proportion of the carotenoids torularhodin, torulene and beta-carotena, as well as the detection of gamma-carotene in the presence of H2O2 and Cu(II) allows to hypothesize that the carotenoids produced by R. mucilaginosa RCL-11 plays different roles in the oxidative stress response of this yeast.