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B Fernandez - One of the best experts on this subject based on the ideXlab platform.
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effects of experimentally induced hyperammonemia on glial fibrillary acidic protein gfap in the Rhombencephalon of goldfish carassius auratus l
Neuroscience Research, 1993Co-Authors: M Rubio, I Suarez, Guillermo Bodega, Rosa M Villalba, B FernandezAbstract:This experimental study was made to know the effect of hyperammonemia on glial fibrillary acidic protein (GFAP) in the glial cells of posterior Rhombencephalon in the goldfish (Carassius auratus L.). Hyperammonemia was induced by elevating the ammonia concentration in the tank water to 0.88 mM with ammonium chloride; the ammonia level in the control tank water was < 0.1 mM. The GFAP levels were measured at 8, 16, 30, 60, 90 and 120 days. GFAP was quantified with a digital analysis system and a transient heterogeneous decrease of GFAP was observed. Hyperammonemia mostly affected GFAP in the astrocyte processes associated with cholinergic pathways. An explanation for the adaptive response to hyperammonemia by fish astrocytes is suggested.
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glial fibrillary acidic protein and vimentin immunohistochemistry in the posterior Rhombencephalon of the iberian barb barbus comiza
Neuroscience Letters, 1992Co-Authors: M Rubio, I Suarez, Guillermo Bodega, B FernandezAbstract:The gustatory centers (vagal lobes and facial lobe) and the tegment of the posterior Rhombencephalon of the Iberian barb (Barbus comiza) have been studied using anti-glial fibrillary acidic protein (anti-GFAP) and anti-vimentin immunohistochemical techniques. GFAP immunoreactivity was found in the tegment and in a part of the vagal lobes while vimentin immunoreactivity was located in the tegment. Two immunopositive cell types were found: ependymocytes and radial astrocytes. Since the distribution of GFAP in the barb Rhombencephalon corresponds with zones previously described as cholinergic, the GFAP-immunopositive radial astrocytes might be involved in acetylcholine metabolism.
M Rubio - One of the best experts on this subject based on the ideXlab platform.
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effects of experimentally induced hyperammonemia on glial fibrillary acidic protein gfap in the Rhombencephalon of goldfish carassius auratus l
Neuroscience Research, 1993Co-Authors: M Rubio, I Suarez, Guillermo Bodega, Rosa M Villalba, B FernandezAbstract:This experimental study was made to know the effect of hyperammonemia on glial fibrillary acidic protein (GFAP) in the glial cells of posterior Rhombencephalon in the goldfish (Carassius auratus L.). Hyperammonemia was induced by elevating the ammonia concentration in the tank water to 0.88 mM with ammonium chloride; the ammonia level in the control tank water was < 0.1 mM. The GFAP levels were measured at 8, 16, 30, 60, 90 and 120 days. GFAP was quantified with a digital analysis system and a transient heterogeneous decrease of GFAP was observed. Hyperammonemia mostly affected GFAP in the astrocyte processes associated with cholinergic pathways. An explanation for the adaptive response to hyperammonemia by fish astrocytes is suggested.
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glial fibrillary acidic protein and vimentin immunohistochemistry in the posterior Rhombencephalon of the iberian barb barbus comiza
Neuroscience Letters, 1992Co-Authors: M Rubio, I Suarez, Guillermo Bodega, B FernandezAbstract:The gustatory centers (vagal lobes and facial lobe) and the tegment of the posterior Rhombencephalon of the Iberian barb (Barbus comiza) have been studied using anti-glial fibrillary acidic protein (anti-GFAP) and anti-vimentin immunohistochemical techniques. GFAP immunoreactivity was found in the tegment and in a part of the vagal lobes while vimentin immunoreactivity was located in the tegment. Two immunopositive cell types were found: ependymocytes and radial astrocytes. Since the distribution of GFAP in the barb Rhombencephalon corresponds with zones previously described as cholinergic, the GFAP-immunopositive radial astrocytes might be involved in acetylcholine metabolism.
Guillermo Bodega - One of the best experts on this subject based on the ideXlab platform.
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effects of experimentally induced hyperammonemia on glial fibrillary acidic protein gfap in the Rhombencephalon of goldfish carassius auratus l
Neuroscience Research, 1993Co-Authors: M Rubio, I Suarez, Guillermo Bodega, Rosa M Villalba, B FernandezAbstract:This experimental study was made to know the effect of hyperammonemia on glial fibrillary acidic protein (GFAP) in the glial cells of posterior Rhombencephalon in the goldfish (Carassius auratus L.). Hyperammonemia was induced by elevating the ammonia concentration in the tank water to 0.88 mM with ammonium chloride; the ammonia level in the control tank water was < 0.1 mM. The GFAP levels were measured at 8, 16, 30, 60, 90 and 120 days. GFAP was quantified with a digital analysis system and a transient heterogeneous decrease of GFAP was observed. Hyperammonemia mostly affected GFAP in the astrocyte processes associated with cholinergic pathways. An explanation for the adaptive response to hyperammonemia by fish astrocytes is suggested.
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glial fibrillary acidic protein and vimentin immunohistochemistry in the posterior Rhombencephalon of the iberian barb barbus comiza
Neuroscience Letters, 1992Co-Authors: M Rubio, I Suarez, Guillermo Bodega, B FernandezAbstract:The gustatory centers (vagal lobes and facial lobe) and the tegment of the posterior Rhombencephalon of the Iberian barb (Barbus comiza) have been studied using anti-glial fibrillary acidic protein (anti-GFAP) and anti-vimentin immunohistochemical techniques. GFAP immunoreactivity was found in the tegment and in a part of the vagal lobes while vimentin immunoreactivity was located in the tegment. Two immunopositive cell types were found: ependymocytes and radial astrocytes. Since the distribution of GFAP in the barb Rhombencephalon corresponds with zones previously described as cholinergic, the GFAP-immunopositive radial astrocytes might be involved in acetylcholine metabolism.
I Suarez - One of the best experts on this subject based on the ideXlab platform.
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effects of experimentally induced hyperammonemia on glial fibrillary acidic protein gfap in the Rhombencephalon of goldfish carassius auratus l
Neuroscience Research, 1993Co-Authors: M Rubio, I Suarez, Guillermo Bodega, Rosa M Villalba, B FernandezAbstract:This experimental study was made to know the effect of hyperammonemia on glial fibrillary acidic protein (GFAP) in the glial cells of posterior Rhombencephalon in the goldfish (Carassius auratus L.). Hyperammonemia was induced by elevating the ammonia concentration in the tank water to 0.88 mM with ammonium chloride; the ammonia level in the control tank water was < 0.1 mM. The GFAP levels were measured at 8, 16, 30, 60, 90 and 120 days. GFAP was quantified with a digital analysis system and a transient heterogeneous decrease of GFAP was observed. Hyperammonemia mostly affected GFAP in the astrocyte processes associated with cholinergic pathways. An explanation for the adaptive response to hyperammonemia by fish astrocytes is suggested.
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glial fibrillary acidic protein and vimentin immunohistochemistry in the posterior Rhombencephalon of the iberian barb barbus comiza
Neuroscience Letters, 1992Co-Authors: M Rubio, I Suarez, Guillermo Bodega, B FernandezAbstract:The gustatory centers (vagal lobes and facial lobe) and the tegment of the posterior Rhombencephalon of the Iberian barb (Barbus comiza) have been studied using anti-glial fibrillary acidic protein (anti-GFAP) and anti-vimentin immunohistochemical techniques. GFAP immunoreactivity was found in the tegment and in a part of the vagal lobes while vimentin immunoreactivity was located in the tegment. Two immunopositive cell types were found: ependymocytes and radial astrocytes. Since the distribution of GFAP in the barb Rhombencephalon corresponds with zones previously described as cholinergic, the GFAP-immunopositive radial astrocytes might be involved in acetylcholine metabolism.
Réjean Dubuc - One of the best experts on this subject based on the ideXlab platform.
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Injection of gabazine into the medOB increases RS neuron responses to ON stimulation.
2018Co-Authors: Gheylen Daghfous, François Auclair, Felix Clotten, Jean-luc Létourneau, Elias Atallah, Jean-patrick Millette, Dominique Derjean, Richard Robitaille, Barbara S. Zielinski, Réjean DubucAbstract:(A) Schematic representation of the brain preparation illustrating the stimulation, injection, and recording sites. (B) Responses of RS neurons to electrical stimulation of the ON (5–15 μA). A local injection of gabazine (0.1 mM) in the medOB increased the RS neuron responses to ON stimulation. Each black trace is a mean of 10 individual responses (colored traces). (C) Univariate scatterplot showing the normalized (as a percentage of control) EPSP amplitude in control, gabazine, and washout conditions for all animals. An asterisk (*) indicates a statistically significant difference at the level p < 0.05, while n.s. indicates the absence of statistically significant difference. The numerical values underlying this figure can be found in S1 Data. Di, diencephalon; EPSP, excitatory postsynaptic potential; LPal, lateral pallium; medOB, medial part of the olfactory bulb; Mes, mesencephalon; ON, olfactory nerve; Rh, Rhombencephalon; RS, reticulospinal.
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respiratory rhythms generated in the lamprey Rhombencephalon
Neuroscience, 2007Co-Authors: B Martel, François Auclair, J P Lund, J C Guimond, Jeanfrancois Gariepy, J Gravel, Arlette Kolta, Réjean DubucAbstract:Brainstem networks generating the respiratory rhythm in lampreys are still not fully characterized. In this study, we described the patterns of respiratory activities and we identified the general location of underlying neural networks. In a semi-intact preparation including the brain and gills, rhythmic discharges were recorded bilaterally with surface electrodes placed over the vagal motoneurons. The main respiratory output driving rhythmic gill movements consisted of short bursts (40.9+/-15.6 ms) of discharge occurring at a frequency of 1.0+/-0.3 Hz. This fast pattern was interrupted by long bursts (506.3+/-174.6 ms) recurring with an average period of 37.4+/-24.9 s. After isolating the brainstem by cutting all cranial nerves, the frequency of the short respiratory bursts did not change significantly, but the slow pattern was less frequent. Local injections of a glutamate agonist (AMPA) and antagonists (6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) or D,L-amino-5-phosphonopentanoic acid (AP5)) were made over different brainstem regions to influence respiratory output. The results were similar in the semi-intact and isolated-brainstem preparations. Unilateral injection of AP5 or CNQX over a rostral rhombencephalic region, lateral to the rostral pole of the trigeminal motor nucleus, decreased the frequency of the fast respiratory rhythm bilaterally or stopped it altogether. Injection of AMPA at the same site increased the rate of the fast respiratory rhythm and decreased the frequency of the slow pattern. The activity recorded in this area was synchronous with that recorded over the vagal motoneurons. After a complete transverse lesion of the brainstem caudal to the trigeminal motor nucleus, the fast rhythm was confined to the rostral area, while only the slow activity persisted in the vagal motoneurons. Our results support the hypothesis that normal breathing depends on the activity of neurons located in the rostral Rhombencephalon in lampreys, whereas the caudal Rhombencephalon generates the slow pattern.
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an anatomical study of brainstem projections to the trigeminal motor nucleus of lampreys
Neuroscience, 1999Co-Authors: H Huard, Réjean Dubuc, J P Lund, D VeilleuxAbstract:This study was undertaken to identify and describe populations of brainstem neurons that project to the area of the nucleus motorius nervi trigemini in lampreys as a first step in the study of neurons that control feeding behavior in this species. To identify these neurons, the retrograde tracer cobalt-lysine was injected into the nucleus motorius nervi trigemini on one side of the in vitro isolated brainstem preparation of seven spawning adult lampreys (Petromyzon marinus). Transport times ranged from 42 to 48 h. Retrogradely labeled neurons were found within the rostral spinal cord, the Rhombencephalon, the mesencephalon and the caudal diencephalon. This study concentrates on the labeled neurons in the Rhombencephalon, since the essential circuits for mastication and swallowing are confined to this region in higher vertebrates. Within the Rhombencephalon, labeled cells were in the nucleus sensibilis nervi trigemini on both sides. A densely packed column of labeled neurons was found medial to the nucleus motorius nervi trigemini on the ipsilateral side, extending further rostrally in the isthmic region. Continuous columns of labeled cells were observed in the lateral reticular formation on each side in the basal plate ventral to rhombencephalic cranial motor nuclei. They extended from the rostral trigeminal region down into the rostral spinal cord. A comparison with data from cats and rats shows that the distribution of neurons that project to the nucleus motorius nervi trigemini is very similar in mammals and in agnathes. We conclude that the organization of the motor command network of the trigeminal system is well preserved throughout phylogeny and that the in vitro isolated brainstem of lampreys should be a useful model for the study of vertebrate feeding behavior.