The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform
Ray Neyland - One of the best experts on this subject based on the ideXlab platform.
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a phylogenetic analysis of large subunit 26s Ribosome DNA sequences suggests that the corsiaceae are polyphyletic
New Zealand Journal of Botany, 2003Co-Authors: Ray Neyland, Melissa K HenniganAbstract:Abstract Corsiaceae is a small family of mycoheterotrophic herbs composed of the genera Arachnitis, Corsia, and Corsiopsis. Due to a lack of DNA sequence data, previous molecular‐based phylogenetic studies have not addressed adequately the position or circumscription of the family. Because mycoheterotrophy results in extreme vegetative reduction that may contribute to homoplasy in the form of character convergence, morphological‐based studies that have addressed these issues may be misleading. Traditionally, Corsiaceae have been grouped with Burmanniaceae and Thismiaceae. However, other than mycoheterotrophy, there are few characters that support this relationship. Additionally, the monophyly of Arachnitis, Corsia, and Corsiopsis has been questioned. The present phylogenetic study, inferred from large‐subunit (26S) Ribosome DNA sequences, suggests that Corsiaceae are polyphyletic. Specifically, Corsia clusters with the order Liliales and Arachnitis appears as sister to Thismia (Thismiaceae) in a larger cl...
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A phylogeny inferred from large-subunit (26S) ribosomal DNA sequences suggests that Burmanniales are polyphyletic
Australian Systematic Botany, 2002Co-Authors: Ray NeylandAbstract:According to several authors, the Burmanniales are an order that includes the families Burmanniaceae, Thismiaceae and Corsiaceae. At present, there is no consensus concerning the circumscription of Burmanniales. All members of Thismiaceae and Corsiaceae are mycoheterotrophic; Burmanniaceae are composed of both mycoheterotrophic and autotrophic species. Other than mycoheterotrophy, there are few characters that unite members of the order. Because mycoheterotrophy has resulted in extreme vegetative reduction that may have contributed to homoplasy in the form of character convergence, the systematics of Burmanniales is problematic. A phylogeny inferred from large-subunit (26S) Ribosome-DNA sequences suggests that the order Burmanniales is polyphyletic. Specifically, the Burmanniaceae appears as strongly supported monophyletic group with Thismia as sister. Corsia is distinct and may be associated with the Liliales.
Yongzhi Li - One of the best experts on this subject based on the ideXlab platform.
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Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans.
BMC Genomics, 2020Co-Authors: Jiaping Wang, Guangxian Zhao, Xiaorui Wu, Chong Xu, Yongzhi LiAbstract:Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study, we aim to explore the phenotypic changes and possible mechanisms of C. albicans after exposure to spaceflight conditions. The effect of C. albicans after carried on the “SJ-10” satellite for 12 days was evaluated by proliferation, morphology, environmental resistance and virulence experiment. The result showed that the proliferation rate, biofilm formation, antioxidant capacity, cytotoxicity and filamentous morphology of C. albicans were increased in the spaceflight group compared to the control group. Proteomics and metabolomics technologies were used to analyze the profiles of proteins and metabolites in C. albicans under spaceflight conditions. Proteomic analysis identified 548 up-regulated proteins involved in the Ribosome, DNA replication, base excision repair and sulfur metabolism in the spaceflight group. Moreover, 332 down-regulated proteins related to metabolic processes were observed. The metabolomic analysis found five differentially expressed metabolites. The combined analysis of proteomic and metabolomic revealed the accumulation of cysteine and methionine in C. albicans after spaceflight. Mechanisms that could explain the results in the phenotypic experiment of C. albicans were found through proteomic and metabolomic analysis. And our data provide an important basis for the assessment of the risk that C. albicans could cause under spaceflight environment.
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Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans
2020Co-Authors: Jiaping Wang, Guangxian Zhao, Xiaorui Wu, Chong Xu, Yongzhi LiAbstract:Abstract Background: Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study, we aim to explore the phenotypic changes and possible mechanisms of C. albicans after exposure to spaceflight conditions. Results: The effect of C. albicans after carried on the "SJ-10" satellite for 12 days was evaluated by proliferation, morphology, environmental resistance and virulence experiment. The result showed that the proliferation rate, biofilm formation, antioxidant capacity, cytotoxicity and filamentous morphology of C. albicans were increased in the spaceflight group compared to the control group. Proteomics and metabolomics technologies were used to analyze the profiles of proteins and metabolites in C. albicans under spaceflight conditions. Proteomic analysis identified 548 up-regulated proteins involved in the Ribosome, DNA replication, base excision repair and sulfur metabolism in the spaceflight group. Moreover, 332 down-regulated proteins related to metabolic processes were observed. The metabolomic analysis found five differentially expressed metabolites. The combined analysis of proteomic and metabolomic revealed the accumulation of cysteine and methionine in C. albicans after spaceflight. Conclusions: Mechanisms that could explain the results in the phenotypic experiment of C. albicans were found through proteomic and metabolomic analysis. And our data provide an important basis for the assessment of the risk that C. albicans could cause under spaceflight environment.
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Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans
2019Co-Authors: Jiaping Wang, Guangxian Zhao, Xiaorui Wu, Chong Xu, Yongzhi LiAbstract:Abstract Background: Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study, we aim to explore the phenotypic changes and possible mechanisms of Candida albicans after exposure to spaceflight conditions. Results: The effect of Candida albicans after carried on the "SJ-10" satellite for 12 days was evaluated by proliferation, morphology, environmental resistance and virulence experiment. The result showed that the proliferation rate, biofilm formation, antioxidant capacity, cytotoxicity and filamentous morphology of Candida albicans were increased in the spaceflight group compared to the control group. Proteomics and metabolomics technologies were used to analyze the profiles of proteins and metabolites in Candida albicans under spaceflight conditions. Proteomic analysis identified 548 up-regulated proteins involved in Ribosome, DNA replication, base excision repair and sulfur metabolism in the spaceflight group. And 332 down-regulated proteins related to metabolic processes were observed. The metabolomic analysis found 5 differentially expressed metabolites. The combined analysis of proteomic and metabolomic revealed the accumulation of cysteine and methionine in Candida albicans after spaceflight. Conclusions: Mechanisms that could explain the results in the phenotypic experiment of Candida albicans were found through proteomic and metabolomic analysis. And our data provide an important basis for the assessment of the risk that Candida albicans could cause under spaceflight environment.
Jiaping Wang - One of the best experts on this subject based on the ideXlab platform.
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Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans.
BMC Genomics, 2020Co-Authors: Jiaping Wang, Guangxian Zhao, Xiaorui Wu, Chong Xu, Yongzhi LiAbstract:Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study, we aim to explore the phenotypic changes and possible mechanisms of C. albicans after exposure to spaceflight conditions. The effect of C. albicans after carried on the “SJ-10” satellite for 12 days was evaluated by proliferation, morphology, environmental resistance and virulence experiment. The result showed that the proliferation rate, biofilm formation, antioxidant capacity, cytotoxicity and filamentous morphology of C. albicans were increased in the spaceflight group compared to the control group. Proteomics and metabolomics technologies were used to analyze the profiles of proteins and metabolites in C. albicans under spaceflight conditions. Proteomic analysis identified 548 up-regulated proteins involved in the Ribosome, DNA replication, base excision repair and sulfur metabolism in the spaceflight group. Moreover, 332 down-regulated proteins related to metabolic processes were observed. The metabolomic analysis found five differentially expressed metabolites. The combined analysis of proteomic and metabolomic revealed the accumulation of cysteine and methionine in C. albicans after spaceflight. Mechanisms that could explain the results in the phenotypic experiment of C. albicans were found through proteomic and metabolomic analysis. And our data provide an important basis for the assessment of the risk that C. albicans could cause under spaceflight environment.
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Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans
2020Co-Authors: Jiaping Wang, Guangxian Zhao, Xiaorui Wu, Chong Xu, Yongzhi LiAbstract:Abstract Background: Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study, we aim to explore the phenotypic changes and possible mechanisms of C. albicans after exposure to spaceflight conditions. Results: The effect of C. albicans after carried on the "SJ-10" satellite for 12 days was evaluated by proliferation, morphology, environmental resistance and virulence experiment. The result showed that the proliferation rate, biofilm formation, antioxidant capacity, cytotoxicity and filamentous morphology of C. albicans were increased in the spaceflight group compared to the control group. Proteomics and metabolomics technologies were used to analyze the profiles of proteins and metabolites in C. albicans under spaceflight conditions. Proteomic analysis identified 548 up-regulated proteins involved in the Ribosome, DNA replication, base excision repair and sulfur metabolism in the spaceflight group. Moreover, 332 down-regulated proteins related to metabolic processes were observed. The metabolomic analysis found five differentially expressed metabolites. The combined analysis of proteomic and metabolomic revealed the accumulation of cysteine and methionine in C. albicans after spaceflight. Conclusions: Mechanisms that could explain the results in the phenotypic experiment of C. albicans were found through proteomic and metabolomic analysis. And our data provide an important basis for the assessment of the risk that C. albicans could cause under spaceflight environment.
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Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans
2019Co-Authors: Jiaping Wang, Guangxian Zhao, Xiaorui Wu, Chong Xu, Yongzhi LiAbstract:Abstract Background: Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study, we aim to explore the phenotypic changes and possible mechanisms of Candida albicans after exposure to spaceflight conditions. Results: The effect of Candida albicans after carried on the "SJ-10" satellite for 12 days was evaluated by proliferation, morphology, environmental resistance and virulence experiment. The result showed that the proliferation rate, biofilm formation, antioxidant capacity, cytotoxicity and filamentous morphology of Candida albicans were increased in the spaceflight group compared to the control group. Proteomics and metabolomics technologies were used to analyze the profiles of proteins and metabolites in Candida albicans under spaceflight conditions. Proteomic analysis identified 548 up-regulated proteins involved in Ribosome, DNA replication, base excision repair and sulfur metabolism in the spaceflight group. And 332 down-regulated proteins related to metabolic processes were observed. The metabolomic analysis found 5 differentially expressed metabolites. The combined analysis of proteomic and metabolomic revealed the accumulation of cysteine and methionine in Candida albicans after spaceflight. Conclusions: Mechanisms that could explain the results in the phenotypic experiment of Candida albicans were found through proteomic and metabolomic analysis. And our data provide an important basis for the assessment of the risk that Candida albicans could cause under spaceflight environment.
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Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans
2019Co-Authors: Jiaping Wang, Guangxian Zhao, Xiaorui Wu, Chong Xu, Li YongzhiAbstract:Abstract Background Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study, we aim to explore the phenotypic changes and possible mechanisms of Candida albicans after exposure to spaceflight conditions.Results The effect of Candida albicans after carried on the "SJ-10" satellite for 12 days was evaluated by proliferation, morphogenic, environmental resistance and virulence experiment. The result showed that the proliferation rate, biofilm formation, antioxidant capacity, cytotoxicity and filamentous morphology of Candida albicans were increased in the spaceflight group compared to the control group. Proteomics and metabolomics technologies were used to analyze the profiles of proteins and metabolites in Candida albicans under spaceflight conditions. Proteomic analysis identified 564 up-regulated proteins involved in Ribosome, DNA replication, base excision repair and sulfur metabolism in the spaceflight group. And 345 down-regulated proteins related to metabolic processes were observed. The metabolomic analysis found 5 different expressed metabolites. The combined analysis of proteomic and metabolomic revealed the accumulation of cysteine and methionine in Candida albicans after spaceflight.Conclusions Mechanisms that could explain the results in the phenotyping experiment of Candida albicans were found through proteomic and metabolomic analysis. And our data provide an important basis for the assessment of the risk that Candida albicans could cause under spaceflight environment.
Hong He - One of the best experts on this subject based on the ideXlab platform.
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A pilot study: a possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus.
BMC Oral Health, 2020Co-Authors: Hong He, Haiping Yang, Qiao Peng, Jiaoer ZhengAbstract:BACKGROUND: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). MATERIALS AND METHODS: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of Ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. RESULTS: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87, 18.75, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SAB were 100%, and inter-groups similarity coefficients, i.e. SAB were less than 30%. CONCLUSIONS: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.
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A pilot study: A possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus
2020Co-Authors: Hong He, Haiping Yang, Qiao Peng, Jiaoer ZhengAbstract:Abstract Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and Methods: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of Ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SAB were 100%, and inter-groups similarity coefficients, i.e. SAB were less than 30%. Conclusions: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.
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A pilot study: a possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus
2019Co-Authors: Hong He, Mengyun Zhang, Haiping Yang, Qiao PengAbstract:Abstract Objectives：This study aimed to investigate the prevalence and genotypic profiles of Candida albicans from patients with oral lichen planus (OLP). Materials andMethods：Genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of Ribosome DNA polymerase chain reaction were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results：The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients S AB were 100%, and inter-groups similarity coefficients S AB were less than 30%. Conclusions：The genotypic evidence of C. albicans in OLP might inferred an endogenous infection and some etiologic sense contributing to professional recognition on the development and progression of OLP for more suitable diagnose and treatment.
Jiaoer Zheng - One of the best experts on this subject based on the ideXlab platform.
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A pilot study: a possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus.
BMC Oral Health, 2020Co-Authors: Hong He, Haiping Yang, Qiao Peng, Jiaoer ZhengAbstract:BACKGROUND: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). MATERIALS AND METHODS: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of Ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. RESULTS: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87, 18.75, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SAB were 100%, and inter-groups similarity coefficients, i.e. SAB were less than 30%. CONCLUSIONS: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.
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A pilot study: A possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus
2020Co-Authors: Hong He, Haiping Yang, Qiao Peng, Jiaoer ZhengAbstract:Abstract Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and Methods: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of Ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SAB were 100%, and inter-groups similarity coefficients, i.e. SAB were less than 30%. Conclusions: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.
