The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Shao Jian Zheng - One of the best experts on this subject based on the ideXlab platform.
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two citrate transporters coordinately regulate citrate secretion from Rice Bean root tip under aluminum stress
Plant Cell and Environment, 2018Co-Authors: Mei Ya Liu, Miguel A Pineros, Leon V Kochian, Shao Jian Zheng, Wei Fan, He Qiang Lou, Wei Wei Chen, Jian Li YangAbstract:Aluminum (Al)-induced organic acid secretion from the root apex is an important Al resistance mechanism. However, it remains unclear how plants fine-tune root organic acid secretion which can contribute significantly to the loss of fixed carbon from the plant. Here, we demonstrate that Al-induced citrate secretion from the Rice Bean root apex is biphasic, consisting of an early phase with low secretion and a later phase of large citrate secretion. We isolated and characterized VuMATE2 as a possible second citrate transporter in Rice Bean functioning in tandem with VuMATE1, which we previously identified. The time-dependent kinetics of VuMATE2 expression correlates well with the kinetics of early phase root citrate secretion. Ectopic expression of VuMATE2 in Arabidopsis resulted in increased root citrate secretion and Al resistance. Electrophysiological analysis of Xenopus oocytes expressing VuMATE2 indicated VuMATE2 mediates anion efflux. However, the expression regulation of VuMATE2 differs from VuMATE1. While a protein translation inhibitor suppressed Al-induced VuMATE1 expression, it releases VuMATE2 expression. Yeast one-hybrid assays demonstrated that a previously identified transcription factor, VuSTOP1, interacts with the VuMATE2 promoter at a GGGAGG cis-acting motif. Thus, we demonstrate that plants adapt to Al toxicity by fine-tuning root citrate secretion with two separate root citrate transport systems.
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Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.
Frontiers in plant science, 2016Co-Authors: Mei Ya Liu, Jian Li Yang, Wei Fan, He Qiang Lou, Shao Jian ZhengAbstract:Rice Bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of Rice Bean to acidic soils where Al stress imposed but Fe deficiency pressure released.
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characterization of an inducible c2h2 type zinc finger transcription factor vustop1 in Rice Bean vigna umbellata reveals differential regulation between low ph and aluminum tolerance mechanisms
New Phytologist, 2015Co-Authors: Jian Li Yang, Wei Fan, He Qiang Lou, Yu Long Gong, Mei Ya Liu, Meng Jie Cao, Yu Liu, Shao Jian ZhengAbstract:Summary The Rice Bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al3+) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from Rice Bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al3 + - and H+-tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al3+ and H+ stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H+ tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H+ tolerance, but only a minor role in Al3+ tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
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Characterization of an inducible C2H2‐type zinc finger transcription factor VuSTOP1 in Rice Bean (Vigna umbellata) reveals differential regulation between low pH and aluminum tolerance mechanisms
The New phytologist, 2015Co-Authors: Wei Fan, Jian Li Yang, He Qiang Lou, Yu Long Gong, Mei Ya Liu, Meng Jie Cao, Yu Liu, Shao Jian ZhengAbstract:Summary The Rice Bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al3+) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from Rice Bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al3 + - and H+-tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al3+ and H+ stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H+ tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H+ tolerance, but only a minor role in Al3+ tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
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a de novo synthesis citrate transporter vigna umbellata multidrug and toxic compound extrusion implicates in al activated citrate efflux in Rice Bean vigna umbellata root apex
Plant Cell and Environment, 2011Co-Authors: Xiao Ying Yang, Jian Li Yang, Yuan Zhou, Miguel A Pineros, Leon V Kochian, Shao Jian ZhengAbstract:Al-activated organic acid anion efflux from roots is an important Al resistance mechanism in plants.We have con- ducted homologous cloning and isolated Vigna umbellata multidrug and toxic compound extrusion (VuMATE), a gene encoding a de novo citrate transporter from Rice Bean. Al treatment up-regulated VuMATE expression in the root apex, but neither in the mature root region nor in the leaf. The degree of up-regulation of VuMATE was both partially Al concentration and time dependent, consistent with the delay in the onset of the Al-induced citrate efflux in Rice Bean roots. While La 3+ moderately induced VuMATE
Wei Fan - One of the best experts on this subject based on the ideXlab platform.
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two citrate transporters coordinately regulate citrate secretion from Rice Bean root tip under aluminum stress
Plant Cell and Environment, 2018Co-Authors: Mei Ya Liu, Miguel A Pineros, Leon V Kochian, Shao Jian Zheng, Wei Fan, He Qiang Lou, Wei Wei Chen, Jian Li YangAbstract:Aluminum (Al)-induced organic acid secretion from the root apex is an important Al resistance mechanism. However, it remains unclear how plants fine-tune root organic acid secretion which can contribute significantly to the loss of fixed carbon from the plant. Here, we demonstrate that Al-induced citrate secretion from the Rice Bean root apex is biphasic, consisting of an early phase with low secretion and a later phase of large citrate secretion. We isolated and characterized VuMATE2 as a possible second citrate transporter in Rice Bean functioning in tandem with VuMATE1, which we previously identified. The time-dependent kinetics of VuMATE2 expression correlates well with the kinetics of early phase root citrate secretion. Ectopic expression of VuMATE2 in Arabidopsis resulted in increased root citrate secretion and Al resistance. Electrophysiological analysis of Xenopus oocytes expressing VuMATE2 indicated VuMATE2 mediates anion efflux. However, the expression regulation of VuMATE2 differs from VuMATE1. While a protein translation inhibitor suppressed Al-induced VuMATE1 expression, it releases VuMATE2 expression. Yeast one-hybrid assays demonstrated that a previously identified transcription factor, VuSTOP1, interacts with the VuMATE2 promoter at a GGGAGG cis-acting motif. Thus, we demonstrate that plants adapt to Al toxicity by fine-tuning root citrate secretion with two separate root citrate transport systems.
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Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.
Frontiers in plant science, 2016Co-Authors: Mei Ya Liu, Jian Li Yang, Wei Fan, He Qiang Lou, Shao Jian ZhengAbstract:Rice Bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of Rice Bean to acidic soils where Al stress imposed but Fe deficiency pressure released.
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characterization of an inducible c2h2 type zinc finger transcription factor vustop1 in Rice Bean vigna umbellata reveals differential regulation between low ph and aluminum tolerance mechanisms
New Phytologist, 2015Co-Authors: Jian Li Yang, Wei Fan, He Qiang Lou, Yu Long Gong, Mei Ya Liu, Meng Jie Cao, Yu Liu, Shao Jian ZhengAbstract:Summary The Rice Bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al3+) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from Rice Bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al3 + - and H+-tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al3+ and H+ stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H+ tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H+ tolerance, but only a minor role in Al3+ tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
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Characterization of an inducible C2H2‐type zinc finger transcription factor VuSTOP1 in Rice Bean (Vigna umbellata) reveals differential regulation between low pH and aluminum tolerance mechanisms
The New phytologist, 2015Co-Authors: Wei Fan, Jian Li Yang, He Qiang Lou, Yu Long Gong, Mei Ya Liu, Meng Jie Cao, Yu Liu, Shao Jian ZhengAbstract:Summary The Rice Bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al3+) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from Rice Bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al3 + - and H+-tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al3+ and H+ stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H+ tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H+ tolerance, but only a minor role in Al3+ tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
Jian Li Yang - One of the best experts on this subject based on the ideXlab platform.
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two citrate transporters coordinately regulate citrate secretion from Rice Bean root tip under aluminum stress
Plant Cell and Environment, 2018Co-Authors: Mei Ya Liu, Miguel A Pineros, Leon V Kochian, Shao Jian Zheng, Wei Fan, He Qiang Lou, Wei Wei Chen, Jian Li YangAbstract:Aluminum (Al)-induced organic acid secretion from the root apex is an important Al resistance mechanism. However, it remains unclear how plants fine-tune root organic acid secretion which can contribute significantly to the loss of fixed carbon from the plant. Here, we demonstrate that Al-induced citrate secretion from the Rice Bean root apex is biphasic, consisting of an early phase with low secretion and a later phase of large citrate secretion. We isolated and characterized VuMATE2 as a possible second citrate transporter in Rice Bean functioning in tandem with VuMATE1, which we previously identified. The time-dependent kinetics of VuMATE2 expression correlates well with the kinetics of early phase root citrate secretion. Ectopic expression of VuMATE2 in Arabidopsis resulted in increased root citrate secretion and Al resistance. Electrophysiological analysis of Xenopus oocytes expressing VuMATE2 indicated VuMATE2 mediates anion efflux. However, the expression regulation of VuMATE2 differs from VuMATE1. While a protein translation inhibitor suppressed Al-induced VuMATE1 expression, it releases VuMATE2 expression. Yeast one-hybrid assays demonstrated that a previously identified transcription factor, VuSTOP1, interacts with the VuMATE2 promoter at a GGGAGG cis-acting motif. Thus, we demonstrate that plants adapt to Al toxicity by fine-tuning root citrate secretion with two separate root citrate transport systems.
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Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.
Frontiers in plant science, 2016Co-Authors: Mei Ya Liu, Jian Li Yang, Wei Fan, He Qiang Lou, Shao Jian ZhengAbstract:Rice Bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of Rice Bean to acidic soils where Al stress imposed but Fe deficiency pressure released.
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characterization of an inducible c2h2 type zinc finger transcription factor vustop1 in Rice Bean vigna umbellata reveals differential regulation between low ph and aluminum tolerance mechanisms
New Phytologist, 2015Co-Authors: Jian Li Yang, Wei Fan, He Qiang Lou, Yu Long Gong, Mei Ya Liu, Meng Jie Cao, Yu Liu, Shao Jian ZhengAbstract:Summary The Rice Bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al3+) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from Rice Bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al3 + - and H+-tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al3+ and H+ stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H+ tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H+ tolerance, but only a minor role in Al3+ tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
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Characterization of an inducible C2H2‐type zinc finger transcription factor VuSTOP1 in Rice Bean (Vigna umbellata) reveals differential regulation between low pH and aluminum tolerance mechanisms
The New phytologist, 2015Co-Authors: Wei Fan, Jian Li Yang, He Qiang Lou, Yu Long Gong, Mei Ya Liu, Meng Jie Cao, Yu Liu, Shao Jian ZhengAbstract:Summary The Rice Bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al3+) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from Rice Bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al3 + - and H+-tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al3+ and H+ stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H+ tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H+ tolerance, but only a minor role in Al3+ tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
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a de novo synthesis citrate transporter vigna umbellata multidrug and toxic compound extrusion implicates in al activated citrate efflux in Rice Bean vigna umbellata root apex
Plant Cell and Environment, 2011Co-Authors: Xiao Ying Yang, Jian Li Yang, Yuan Zhou, Miguel A Pineros, Leon V Kochian, Shao Jian ZhengAbstract:Al-activated organic acid anion efflux from roots is an important Al resistance mechanism in plants.We have con- ducted homologous cloning and isolated Vigna umbellata multidrug and toxic compound extrusion (VuMATE), a gene encoding a de novo citrate transporter from Rice Bean. Al treatment up-regulated VuMATE expression in the root apex, but neither in the mature root region nor in the leaf. The degree of up-regulation of VuMATE was both partially Al concentration and time dependent, consistent with the delay in the onset of the Al-induced citrate efflux in Rice Bean roots. While La 3+ moderately induced VuMATE
Mei Ya Liu - One of the best experts on this subject based on the ideXlab platform.
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two citrate transporters coordinately regulate citrate secretion from Rice Bean root tip under aluminum stress
Plant Cell and Environment, 2018Co-Authors: Mei Ya Liu, Miguel A Pineros, Leon V Kochian, Shao Jian Zheng, Wei Fan, He Qiang Lou, Wei Wei Chen, Jian Li YangAbstract:Aluminum (Al)-induced organic acid secretion from the root apex is an important Al resistance mechanism. However, it remains unclear how plants fine-tune root organic acid secretion which can contribute significantly to the loss of fixed carbon from the plant. Here, we demonstrate that Al-induced citrate secretion from the Rice Bean root apex is biphasic, consisting of an early phase with low secretion and a later phase of large citrate secretion. We isolated and characterized VuMATE2 as a possible second citrate transporter in Rice Bean functioning in tandem with VuMATE1, which we previously identified. The time-dependent kinetics of VuMATE2 expression correlates well with the kinetics of early phase root citrate secretion. Ectopic expression of VuMATE2 in Arabidopsis resulted in increased root citrate secretion and Al resistance. Electrophysiological analysis of Xenopus oocytes expressing VuMATE2 indicated VuMATE2 mediates anion efflux. However, the expression regulation of VuMATE2 differs from VuMATE1. While a protein translation inhibitor suppressed Al-induced VuMATE1 expression, it releases VuMATE2 expression. Yeast one-hybrid assays demonstrated that a previously identified transcription factor, VuSTOP1, interacts with the VuMATE2 promoter at a GGGAGG cis-acting motif. Thus, we demonstrate that plants adapt to Al toxicity by fine-tuning root citrate secretion with two separate root citrate transport systems.
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Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.
Frontiers in plant science, 2016Co-Authors: Mei Ya Liu, Jian Li Yang, Wei Fan, He Qiang Lou, Shao Jian ZhengAbstract:Rice Bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of Rice Bean to acidic soils where Al stress imposed but Fe deficiency pressure released.
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characterization of an inducible c2h2 type zinc finger transcription factor vustop1 in Rice Bean vigna umbellata reveals differential regulation between low ph and aluminum tolerance mechanisms
New Phytologist, 2015Co-Authors: Jian Li Yang, Wei Fan, He Qiang Lou, Yu Long Gong, Mei Ya Liu, Meng Jie Cao, Yu Liu, Shao Jian ZhengAbstract:Summary The Rice Bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al3+) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from Rice Bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al3 + - and H+-tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al3+ and H+ stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H+ tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H+ tolerance, but only a minor role in Al3+ tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
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Characterization of an inducible C2H2‐type zinc finger transcription factor VuSTOP1 in Rice Bean (Vigna umbellata) reveals differential regulation between low pH and aluminum tolerance mechanisms
The New phytologist, 2015Co-Authors: Wei Fan, Jian Li Yang, He Qiang Lou, Yu Long Gong, Mei Ya Liu, Meng Jie Cao, Yu Liu, Shao Jian ZhengAbstract:Summary The Rice Bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al3+) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from Rice Bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al3 + - and H+-tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al3+ and H+ stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H+ tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H+ tolerance, but only a minor role in Al3+ tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
He Qiang Lou - One of the best experts on this subject based on the ideXlab platform.
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two citrate transporters coordinately regulate citrate secretion from Rice Bean root tip under aluminum stress
Plant Cell and Environment, 2018Co-Authors: Mei Ya Liu, Miguel A Pineros, Leon V Kochian, Shao Jian Zheng, Wei Fan, He Qiang Lou, Wei Wei Chen, Jian Li YangAbstract:Aluminum (Al)-induced organic acid secretion from the root apex is an important Al resistance mechanism. However, it remains unclear how plants fine-tune root organic acid secretion which can contribute significantly to the loss of fixed carbon from the plant. Here, we demonstrate that Al-induced citrate secretion from the Rice Bean root apex is biphasic, consisting of an early phase with low secretion and a later phase of large citrate secretion. We isolated and characterized VuMATE2 as a possible second citrate transporter in Rice Bean functioning in tandem with VuMATE1, which we previously identified. The time-dependent kinetics of VuMATE2 expression correlates well with the kinetics of early phase root citrate secretion. Ectopic expression of VuMATE2 in Arabidopsis resulted in increased root citrate secretion and Al resistance. Electrophysiological analysis of Xenopus oocytes expressing VuMATE2 indicated VuMATE2 mediates anion efflux. However, the expression regulation of VuMATE2 differs from VuMATE1. While a protein translation inhibitor suppressed Al-induced VuMATE1 expression, it releases VuMATE2 expression. Yeast one-hybrid assays demonstrated that a previously identified transcription factor, VuSTOP1, interacts with the VuMATE2 promoter at a GGGAGG cis-acting motif. Thus, we demonstrate that plants adapt to Al toxicity by fine-tuning root citrate secretion with two separate root citrate transport systems.
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Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.
Frontiers in plant science, 2016Co-Authors: Mei Ya Liu, Jian Li Yang, Wei Fan, He Qiang Lou, Shao Jian ZhengAbstract:Rice Bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of Rice Bean to acidic soils where Al stress imposed but Fe deficiency pressure released.
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characterization of an inducible c2h2 type zinc finger transcription factor vustop1 in Rice Bean vigna umbellata reveals differential regulation between low ph and aluminum tolerance mechanisms
New Phytologist, 2015Co-Authors: Jian Li Yang, Wei Fan, He Qiang Lou, Yu Long Gong, Mei Ya Liu, Meng Jie Cao, Yu Liu, Shao Jian ZhengAbstract:Summary The Rice Bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al3+) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from Rice Bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al3 + - and H+-tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al3+ and H+ stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H+ tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H+ tolerance, but only a minor role in Al3+ tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
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Characterization of an inducible C2H2‐type zinc finger transcription factor VuSTOP1 in Rice Bean (Vigna umbellata) reveals differential regulation between low pH and aluminum tolerance mechanisms
The New phytologist, 2015Co-Authors: Wei Fan, Jian Li Yang, He Qiang Lou, Yu Long Gong, Mei Ya Liu, Meng Jie Cao, Yu Liu, Shao Jian ZhengAbstract:Summary The Rice Bean (Vigna umbellata) root apex specifically secretes citrate through expression activation of Vigna umbellata Multidrug and Toxic Compound Extrusion 1 (VuMATE1) under aluminum (Al3+) stress. However, the underlying mechanisms regulating VuMATE1 expression remain unknown. We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)-like protein, VuSTOP1, from Rice Bean. The role of VuSTOP1 in regulating VuMATE1 expression was investigated using the yeast one-hybrid assay. We characterized the function of VuSTOP1 in Al3 + - and H+-tolerance using in planta complementation assays. We demonstrated that VuSTOP1 has transactivation potential. We found that VuSTOP1 expression is inducible by Al3+ and H+ stress. However, although VuSTOP1 binds to the promoter of VuMATE1, the inconsistent tissue localization patterns of VuSTOP1 and VuMATE1 preclude VuSTOP1 as the major factor regulating VuMATE1 expression. In addition, when a protein translation inhibitor increased expression of VuSTOP1, VuMATE1 expression was inhibited. In planta complementation assay demonstrated that VuSTOP1 could fully restore expression of genes involved in H+ tolerance, but could only partially restore expression of AtMATE. We conclude that VuSTOP1 plays a major role in H+ tolerance, but only a minor role in Al3+ tolerance. The differential transcriptional regulation of VuSTOP1 and VuMATE1 reveals a complex regulatory system controlling VuMATE1 expression.
