The Experts below are selected from a list of 12 Experts worldwide ranked by ideXlab platform
Kevin R Macaluso - One of the best experts on this subject based on the ideXlab platform.
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feeding by amblyomma maculatum acari ixodidae enhances rickettsia parkeri rickettsiales Rickettsiaceae Infection in the skin
Journal of Medical Entomology, 2014Co-Authors: Britton J Grasperge, Timothy W Morgan, Christopher D Paddock, Karin E Peterson, Kevin R MacalusoAbstract:ABSTRACT Rickettsia parkeri Luckman (Rickettsiales: Rickettsiaceae), a member of the spotted fever group of Rickettsia, is the tick-borne causative agent of a newly recognized, eschar-associated rickettsiosis. Because of its relatively recent designation as a pathogen, few studies have examined the pathogenesis of transmission of R. parkeri to the vertebrate host. To further elucidate the role of tick feeding in rickettsial Infection of vertebrates, nymphal Amblyomma maculatum Koch (Acari: Ixodidae) were fed on C3H/HeJ mice intraderm ally inoculated with R. parkeri (Portsmouth strain). The ticks were allowed to feed to repletion, at which time samples were taken for histopathology, immunohistochemistry (IHC), quantitative polymerase chain reaction (qPCR) for rickettsial quantification, and reverse transcriptase polymerase chain reaction (RT-PCR) for expression of Itgax, Mcpl, and Il1β. The group of mice that received intradermal inoculation of R. parkeri with tick feeding displayed significant increases i...
Britton J Grasperge - One of the best experts on this subject based on the ideXlab platform.
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feeding by amblyomma maculatum acari ixodidae enhances rickettsia parkeri rickettsiales Rickettsiaceae Infection in the skin
Journal of Medical Entomology, 2014Co-Authors: Britton J Grasperge, Timothy W Morgan, Christopher D Paddock, Karin E Peterson, Kevin R MacalusoAbstract:ABSTRACT Rickettsia parkeri Luckman (Rickettsiales: Rickettsiaceae), a member of the spotted fever group of Rickettsia, is the tick-borne causative agent of a newly recognized, eschar-associated rickettsiosis. Because of its relatively recent designation as a pathogen, few studies have examined the pathogenesis of transmission of R. parkeri to the vertebrate host. To further elucidate the role of tick feeding in rickettsial Infection of vertebrates, nymphal Amblyomma maculatum Koch (Acari: Ixodidae) were fed on C3H/HeJ mice intraderm ally inoculated with R. parkeri (Portsmouth strain). The ticks were allowed to feed to repletion, at which time samples were taken for histopathology, immunohistochemistry (IHC), quantitative polymerase chain reaction (qPCR) for rickettsial quantification, and reverse transcriptase polymerase chain reaction (RT-PCR) for expression of Itgax, Mcpl, and Il1β. The group of mice that received intradermal inoculation of R. parkeri with tick feeding displayed significant increases i...
Michael L Levin - One of the best experts on this subject based on the ideXlab platform.
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effect of rickettsia rickettsii rickettsiales Rickettsiaceae Infection on the biological parameters and survival of its tick vector dermacentor variabilis acari ixodidae
Journal of Medical Entomology, 2016Co-Authors: Lauren Schumacher, Alyssa Snellgrove, Michael L LevinAbstract:Rocky Mountain spotted fever, caused by Rickettsia rickettsii , is a potentially fatal tick-borne disease spread from North America to Argentina. The major vectors of R. rickettsii in the United States are Dermacentor andersoni Stiles and Dermacentor variabilis (Say). It is generally believed that vector ticks serve as major reservoirs of R. rickettsii in nature; however, the ability of ticks to support the indefinite perpetuation of R. rickettsii has been challenged by reports of deleterious effects of rickettsial Infection on D. andersoni. To better elucidate the relationship of the pathogen with D. variabilis , we assessed the effects of R. rickettsii on the survival, fertility, and fecundity of D. variabilis. We used an isolate of R. rickettsii (Di-6), originally acquired from an opossum caught in Virginia, and ticks from a laboratory colony established from adult D. variabilis also collected in Virginia. Overall, Infection with R. rickettsii protracted the feeding periods of all life stages of ticks. Infected nymphal and adult ticks experienced a slight decrease in feeding success compared with the uninfected colony, but neither larval nor nymphal molting success was affected. Infected females reached smaller engorgement weights, were less efficient in conversion of bloodmeal into eggs, and produced smaller egg clutches with a lower proportion of eggs hatching. However, no sudden die-off was observed among infected ticks, and longevity was not decreased due to R. rickettsii Infection in any stage. Although Infection with the studied isolate of R. rickettsii caused slight decrease in fecundity in sympatric vector ticks, no obvious deleterious effects were observed.
Karin E Peterson - One of the best experts on this subject based on the ideXlab platform.
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feeding by amblyomma maculatum acari ixodidae enhances rickettsia parkeri rickettsiales Rickettsiaceae Infection in the skin
Journal of Medical Entomology, 2014Co-Authors: Britton J Grasperge, Timothy W Morgan, Christopher D Paddock, Karin E Peterson, Kevin R MacalusoAbstract:ABSTRACT Rickettsia parkeri Luckman (Rickettsiales: Rickettsiaceae), a member of the spotted fever group of Rickettsia, is the tick-borne causative agent of a newly recognized, eschar-associated rickettsiosis. Because of its relatively recent designation as a pathogen, few studies have examined the pathogenesis of transmission of R. parkeri to the vertebrate host. To further elucidate the role of tick feeding in rickettsial Infection of vertebrates, nymphal Amblyomma maculatum Koch (Acari: Ixodidae) were fed on C3H/HeJ mice intraderm ally inoculated with R. parkeri (Portsmouth strain). The ticks were allowed to feed to repletion, at which time samples were taken for histopathology, immunohistochemistry (IHC), quantitative polymerase chain reaction (qPCR) for rickettsial quantification, and reverse transcriptase polymerase chain reaction (RT-PCR) for expression of Itgax, Mcpl, and Il1β. The group of mice that received intradermal inoculation of R. parkeri with tick feeding displayed significant increases i...
Timothy W Morgan - One of the best experts on this subject based on the ideXlab platform.
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feeding by amblyomma maculatum acari ixodidae enhances rickettsia parkeri rickettsiales Rickettsiaceae Infection in the skin
Journal of Medical Entomology, 2014Co-Authors: Britton J Grasperge, Timothy W Morgan, Christopher D Paddock, Karin E Peterson, Kevin R MacalusoAbstract:ABSTRACT Rickettsia parkeri Luckman (Rickettsiales: Rickettsiaceae), a member of the spotted fever group of Rickettsia, is the tick-borne causative agent of a newly recognized, eschar-associated rickettsiosis. Because of its relatively recent designation as a pathogen, few studies have examined the pathogenesis of transmission of R. parkeri to the vertebrate host. To further elucidate the role of tick feeding in rickettsial Infection of vertebrates, nymphal Amblyomma maculatum Koch (Acari: Ixodidae) were fed on C3H/HeJ mice intraderm ally inoculated with R. parkeri (Portsmouth strain). The ticks were allowed to feed to repletion, at which time samples were taken for histopathology, immunohistochemistry (IHC), quantitative polymerase chain reaction (qPCR) for rickettsial quantification, and reverse transcriptase polymerase chain reaction (RT-PCR) for expression of Itgax, Mcpl, and Il1β. The group of mice that received intradermal inoculation of R. parkeri with tick feeding displayed significant increases i...
