The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
Tomokazu Matsue - One of the best experts on this subject based on the ideXlab platform.
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electrochemical chip integrating scalable Ring Ring Electrode array to detect secreted alkaline phosphatase
Analyst, 2011Co-Authors: Michiaki Takeda, Hitoshi Shiku, Kosuke Ino, Tomokazu MatsueAbstract:An electrochemical platform for parallel monitoRing of secreted alkaline phosphatase (SEAP) has been microfabricated on a device with a mammalian-cell array chip. A 4 × 4 Ring–Ring Electrode array was designed at the rim of the round cellular pattern with a diameter of 270 μm. Electrochemical characterization was carried out, and it was found that the collection efficiency was about 50% in dual mode when the inner-Ring and the outer-Ring Electrodes were selected as the collector and generator Electrodes, respectively. The current amplification ratio for the dual mode normal to single mode was 2.84. SEAP expressing from the cells was parallelly monitored by using a multiplexer switching system at the 16 round cellular spots. The reduction current for HeLa cells transfected with plasmid encoding SEAP observed at the collector outer Ring Electrode was found to be significantly higher than that for wild-type HeLa. Finally, the top of the microwell with the round cellular pattern was covered with a poly(dimethylsiloxane) block for 5 min to accumulate the secreted enzyme and the product of the enzyme reaction so that further signal enhancement could be observed.
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Electrochemical chip integrating scalable Ring–Ring Electrode array to detect secreted alkaline phosphatase
The Analyst, 2011Co-Authors: Michiaki Takeda, Hitoshi Shiku, Kosuke Ino, Tomokazu MatsueAbstract:An electrochemical platform for parallel monitoRing of secreted alkaline phosphatase (SEAP) has been microfabricated on a device with a mammalian-cell array chip. A 4 × 4 Ring–Ring Electrode array was designed at the rim of the round cellular pattern with a diameter of 270 μm. Electrochemical characterization was carried out, and it was found that the collection efficiency was about 50% in dual mode when the inner-Ring and the outer-Ring Electrodes were selected as the collector and generator Electrodes, respectively. The current amplification ratio for the dual mode normal to single mode was 2.84. SEAP expressing from the cells was parallelly monitored by using a multiplexer switching system at the 16 round cellular spots. The reduction current for HeLa cells transfected with plasmid encoding SEAP observed at the collector outer Ring Electrode was found to be significantly higher than that for wild-type HeLa. Finally, the top of the microwell with the round cellular pattern was covered with a poly(dimethylsiloxane) block for 5 min to accumulate the secreted enzyme and the product of the enzyme reaction so that further signal enhancement could be observed.
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transfected single cell imaging by scanning electrochemical optical microscopy with shear force feedback regulation
Analytical Chemistry, 2009Co-Authors: Yasufumi Takahashi, Hitoshi Shiku, Tatsuya Murata, Tomoyuki Yasukawa, Tomokazu MatsueAbstract:Gene-transfected single HeLa cells were characterized using a scanning electrochemical/optical microscope (SECM/OM) system with shear-force-based probe-sample distance regulation to simultaneously capture electrochemical, fluorescent, and topographic images. The outer and inner states of single living cells were obtained as electrochemical and fluorescent signals, respectively, by using an optical fiber-nanoElectrode probe. A focused ion beam (FIB) was used to mill the optical aperture and the Ring Electrode at the probe apex (the inner and outer radii of the Ring Electrode were 37 and 112 nm, respectively). To apply an appropriate shear force between the probe tip and the living cell surface, we optimized the amplitude of oscillation of the tuning fork to which the probe was attached. Field-programmable gate arrays (FPGA) were adopted to drastically increase the feedback speed of the tip−sample distance regulation, shorten the scanning time for imaging, and enhance the accuracy and quality of the living ...
Hitoshi Shiku - One of the best experts on this subject based on the ideXlab platform.
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electrochemical chip integrating scalable Ring Ring Electrode array to detect secreted alkaline phosphatase
Analyst, 2011Co-Authors: Michiaki Takeda, Hitoshi Shiku, Kosuke Ino, Tomokazu MatsueAbstract:An electrochemical platform for parallel monitoRing of secreted alkaline phosphatase (SEAP) has been microfabricated on a device with a mammalian-cell array chip. A 4 × 4 Ring–Ring Electrode array was designed at the rim of the round cellular pattern with a diameter of 270 μm. Electrochemical characterization was carried out, and it was found that the collection efficiency was about 50% in dual mode when the inner-Ring and the outer-Ring Electrodes were selected as the collector and generator Electrodes, respectively. The current amplification ratio for the dual mode normal to single mode was 2.84. SEAP expressing from the cells was parallelly monitored by using a multiplexer switching system at the 16 round cellular spots. The reduction current for HeLa cells transfected with plasmid encoding SEAP observed at the collector outer Ring Electrode was found to be significantly higher than that for wild-type HeLa. Finally, the top of the microwell with the round cellular pattern was covered with a poly(dimethylsiloxane) block for 5 min to accumulate the secreted enzyme and the product of the enzyme reaction so that further signal enhancement could be observed.
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Electrochemical chip integrating scalable Ring–Ring Electrode array to detect secreted alkaline phosphatase
The Analyst, 2011Co-Authors: Michiaki Takeda, Hitoshi Shiku, Kosuke Ino, Tomokazu MatsueAbstract:An electrochemical platform for parallel monitoRing of secreted alkaline phosphatase (SEAP) has been microfabricated on a device with a mammalian-cell array chip. A 4 × 4 Ring–Ring Electrode array was designed at the rim of the round cellular pattern with a diameter of 270 μm. Electrochemical characterization was carried out, and it was found that the collection efficiency was about 50% in dual mode when the inner-Ring and the outer-Ring Electrodes were selected as the collector and generator Electrodes, respectively. The current amplification ratio for the dual mode normal to single mode was 2.84. SEAP expressing from the cells was parallelly monitored by using a multiplexer switching system at the 16 round cellular spots. The reduction current for HeLa cells transfected with plasmid encoding SEAP observed at the collector outer Ring Electrode was found to be significantly higher than that for wild-type HeLa. Finally, the top of the microwell with the round cellular pattern was covered with a poly(dimethylsiloxane) block for 5 min to accumulate the secreted enzyme and the product of the enzyme reaction so that further signal enhancement could be observed.
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transfected single cell imaging by scanning electrochemical optical microscopy with shear force feedback regulation
Analytical Chemistry, 2009Co-Authors: Yasufumi Takahashi, Hitoshi Shiku, Tatsuya Murata, Tomoyuki Yasukawa, Tomokazu MatsueAbstract:Gene-transfected single HeLa cells were characterized using a scanning electrochemical/optical microscope (SECM/OM) system with shear-force-based probe-sample distance regulation to simultaneously capture electrochemical, fluorescent, and topographic images. The outer and inner states of single living cells were obtained as electrochemical and fluorescent signals, respectively, by using an optical fiber-nanoElectrode probe. A focused ion beam (FIB) was used to mill the optical aperture and the Ring Electrode at the probe apex (the inner and outer radii of the Ring Electrode were 37 and 112 nm, respectively). To apply an appropriate shear force between the probe tip and the living cell surface, we optimized the amplitude of oscillation of the tuning fork to which the probe was attached. Field-programmable gate arrays (FPGA) were adopted to drastically increase the feedback speed of the tip−sample distance regulation, shorten the scanning time for imaging, and enhance the accuracy and quality of the living ...
Michiaki Takeda - One of the best experts on this subject based on the ideXlab platform.
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electrochemical chip integrating scalable Ring Ring Electrode array to detect secreted alkaline phosphatase
Analyst, 2011Co-Authors: Michiaki Takeda, Hitoshi Shiku, Kosuke Ino, Tomokazu MatsueAbstract:An electrochemical platform for parallel monitoRing of secreted alkaline phosphatase (SEAP) has been microfabricated on a device with a mammalian-cell array chip. A 4 × 4 Ring–Ring Electrode array was designed at the rim of the round cellular pattern with a diameter of 270 μm. Electrochemical characterization was carried out, and it was found that the collection efficiency was about 50% in dual mode when the inner-Ring and the outer-Ring Electrodes were selected as the collector and generator Electrodes, respectively. The current amplification ratio for the dual mode normal to single mode was 2.84. SEAP expressing from the cells was parallelly monitored by using a multiplexer switching system at the 16 round cellular spots. The reduction current for HeLa cells transfected with plasmid encoding SEAP observed at the collector outer Ring Electrode was found to be significantly higher than that for wild-type HeLa. Finally, the top of the microwell with the round cellular pattern was covered with a poly(dimethylsiloxane) block for 5 min to accumulate the secreted enzyme and the product of the enzyme reaction so that further signal enhancement could be observed.
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Electrochemical chip integrating scalable Ring–Ring Electrode array to detect secreted alkaline phosphatase
The Analyst, 2011Co-Authors: Michiaki Takeda, Hitoshi Shiku, Kosuke Ino, Tomokazu MatsueAbstract:An electrochemical platform for parallel monitoRing of secreted alkaline phosphatase (SEAP) has been microfabricated on a device with a mammalian-cell array chip. A 4 × 4 Ring–Ring Electrode array was designed at the rim of the round cellular pattern with a diameter of 270 μm. Electrochemical characterization was carried out, and it was found that the collection efficiency was about 50% in dual mode when the inner-Ring and the outer-Ring Electrodes were selected as the collector and generator Electrodes, respectively. The current amplification ratio for the dual mode normal to single mode was 2.84. SEAP expressing from the cells was parallelly monitored by using a multiplexer switching system at the 16 round cellular spots. The reduction current for HeLa cells transfected with plasmid encoding SEAP observed at the collector outer Ring Electrode was found to be significantly higher than that for wild-type HeLa. Finally, the top of the microwell with the round cellular pattern was covered with a poly(dimethylsiloxane) block for 5 min to accumulate the secreted enzyme and the product of the enzyme reaction so that further signal enhancement could be observed.
John L. Hudson - One of the best experts on this subject based on the ideXlab platform.
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Spatiotemporal patterns and symmetry breaking on a Ring Electrode.
Physical Review E, 2001Co-Authors: B. J. Green, John L. HudsonAbstract:A series of experiments on a Ring Electrode with changes in a parameter, the applied potential, are described. Spatiotemporal patterns are investigated in a region of parameter space in which relaxation oscillations occur. The simplest state is a period 2Pi oscillation that has full O(2) symmetry so that at each instant the pattern is unchanged by rotations or reflections of the Ring. With change in parameter a spatiotemporal period doubling occurs to period 4Pi. This is followed by a symmetry breaking to another state with period 4Pi and subsequently by a second period doubling to period 8Pi. Proper orthogonal decomposition is used as an aid in elucidating the nature of the transitions.
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Negative coupling duRing oscillatory pattern formation on a Ring Electrode
The Journal of Chemical Physics, 1999Co-Authors: Johannes Christoph, Markus Eiswirth, R. D. Otterstedt, Nils I. Jaeger, John L. HudsonAbstract:Pattern formation duRing the oscillatory electrodissolution of Co was studied using a Co Ring Electrode with a small reference Electrode at a short distance in the center of the Ring. Traveling pulses as well as source points (one-dimensional target patterns) were observed. These findings could be reproduced using a reaction-migration equation (RME) the coupling function of which was derived for the particular geometry from basic potential theory and was found to become negative for increasing distance. DuRing pattern formation (i.e., for inhomogeneous potential distribution) the potential drop across the double layer could actually exceed the external applied voltage giving direct evidence for negative coupling.
Kosuke Ino - One of the best experts on this subject based on the ideXlab platform.
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electrochemical chip integrating scalable Ring Ring Electrode array to detect secreted alkaline phosphatase
Analyst, 2011Co-Authors: Michiaki Takeda, Hitoshi Shiku, Kosuke Ino, Tomokazu MatsueAbstract:An electrochemical platform for parallel monitoRing of secreted alkaline phosphatase (SEAP) has been microfabricated on a device with a mammalian-cell array chip. A 4 × 4 Ring–Ring Electrode array was designed at the rim of the round cellular pattern with a diameter of 270 μm. Electrochemical characterization was carried out, and it was found that the collection efficiency was about 50% in dual mode when the inner-Ring and the outer-Ring Electrodes were selected as the collector and generator Electrodes, respectively. The current amplification ratio for the dual mode normal to single mode was 2.84. SEAP expressing from the cells was parallelly monitored by using a multiplexer switching system at the 16 round cellular spots. The reduction current for HeLa cells transfected with plasmid encoding SEAP observed at the collector outer Ring Electrode was found to be significantly higher than that for wild-type HeLa. Finally, the top of the microwell with the round cellular pattern was covered with a poly(dimethylsiloxane) block for 5 min to accumulate the secreted enzyme and the product of the enzyme reaction so that further signal enhancement could be observed.
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Electrochemical chip integrating scalable Ring–Ring Electrode array to detect secreted alkaline phosphatase
The Analyst, 2011Co-Authors: Michiaki Takeda, Hitoshi Shiku, Kosuke Ino, Tomokazu MatsueAbstract:An electrochemical platform for parallel monitoRing of secreted alkaline phosphatase (SEAP) has been microfabricated on a device with a mammalian-cell array chip. A 4 × 4 Ring–Ring Electrode array was designed at the rim of the round cellular pattern with a diameter of 270 μm. Electrochemical characterization was carried out, and it was found that the collection efficiency was about 50% in dual mode when the inner-Ring and the outer-Ring Electrodes were selected as the collector and generator Electrodes, respectively. The current amplification ratio for the dual mode normal to single mode was 2.84. SEAP expressing from the cells was parallelly monitored by using a multiplexer switching system at the 16 round cellular spots. The reduction current for HeLa cells transfected with plasmid encoding SEAP observed at the collector outer Ring Electrode was found to be significantly higher than that for wild-type HeLa. Finally, the top of the microwell with the round cellular pattern was covered with a poly(dimethylsiloxane) block for 5 min to accumulate the secreted enzyme and the product of the enzyme reaction so that further signal enhancement could be observed.
