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Władysław Cabaj - One of the best experts on this subject based on the ideXlab platform.
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First Toxoplasma gondii isolate from an aborted foetus of European bison (Bison bonasus bonasus L.).
Parasitology Research, 2017Co-Authors: Bożena Moskwa, Michał K. Krzysiak, Katarzyna Goździk, Justyna Bień, Aleksandra Kornacka, Aleksandra Cybulska, Katarína Reiterová, Władysław CabajAbstract:The study was performed on a male European bison (Bison bonasus bonasus L.) foetus spontaneously aborted at the fourth or fifth month of pregnancy in the Bialowieza Forest. Serum samples from the foetus and mother revealed the presence of antibodies against T. gondii (S/P% = 88% and 75%, respectively). Mobile extracellular tachyzoites were first observed in a Vero cell culture, 110 days following inoculation of brain homogenate. PCR amplification with TGR1E1 and TGR1E2 primers confirmed the presence of T. gondii DNA, which was classified as Type I by PCR-RFLP genotyping. The sequences of 18S ribosomal RNA (18S rRNA) and 5.8S ribosomal RNA (5.8S rRNA) genes; inteRNAl transcribed spacer 1 (ITS1) and inteRNAl transcribed spacer 2 (ITS2), obtained from T. gondii isolate, have been deposited in GenBank (accession number KX459518.1). This is the first in vitro isolation and molecular identification of T. gondii from an aborted European bison foetus. The origin of this protozoan isolate indicates that the species is a significant threat to the European bison conservation program implemented in the Bialowieza Forest.
Vladimir A Richter - One of the best experts on this subject based on the ideXlab platform.
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deamination of adenosines in extracellular RNA spontaneously inteRNAlized by human cells
Annals of the New York Academy of Sciences, 2008Co-Authors: Elena V Kuligina, Oksana V Vratskih, Dmitry V Semenov, V A Matveeva, Vladimir A RichterAbstract:Ribonucleic acids circulating in mammalian extracellular fluids as well as RNAs accumulating in culture medium condensed by mammalian cells are inteRNAlized by acceptor cells and distributed among cellular compartments. The inteRNAlized RNA can be involved in the induction and regulation of cellular processes as guide or signaling molecules. The inteRNAlization of RNA may be accompanied by covalent modifications influencing the stability and functionality of this RNA. To analyze nucleotide modifications introduced by human cells in inteRNAlized extracellular RNA, 5.8S rRNA of S. cerevisiae was used. It was shown that 5.8S rRNA of S. cerevisiae is captured by human adenocarcinoma MCF-7 cells from culture medium and delivered to cytoplasm and nuclei. Most of the inteRNAlized RNA was hydrolyzed to mono- and oligonucleotides. Full-length RNA uptake was detected by RT-PCR in the cytoplasm and in nuclei after the pulse addition of RNA to the culture medium. 5'-Inosine was the only detectable modified nucleotide in the hydrolysate of cell-inteRNAlized RNAs. Consequently, extracellular RNAs entering human cells were subjected to partial adenosine deamination. Sequencing of cDNA confirmed that full-length extracellular RNA that accumulated in the nucleus, but not in the cytoplasm, was partially edited by adenosine deaminases. The deamination revealed in nuclear-stored, full-length RNA was site-specific. Adenosines edited in S. cerevisiae 5.8S rRNA are stack-closing A-U pairs A53 and A96, as well as unpaired A44 and A65. Our data emphasize the participation of adenosine deaminases in capturing and intracellular trafficking of inteRNAlized RNAs.
Bożena Moskwa - One of the best experts on this subject based on the ideXlab platform.
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First Toxoplasma gondii isolate from an aborted foetus of European bison (Bison bonasus bonasus L.).
Parasitology Research, 2017Co-Authors: Bożena Moskwa, Michał K. Krzysiak, Katarzyna Goździk, Justyna Bień, Aleksandra Kornacka, Aleksandra Cybulska, Katarína Reiterová, Władysław CabajAbstract:The study was performed on a male European bison (Bison bonasus bonasus L.) foetus spontaneously aborted at the fourth or fifth month of pregnancy in the Bialowieza Forest. Serum samples from the foetus and mother revealed the presence of antibodies against T. gondii (S/P% = 88% and 75%, respectively). Mobile extracellular tachyzoites were first observed in a Vero cell culture, 110 days following inoculation of brain homogenate. PCR amplification with TGR1E1 and TGR1E2 primers confirmed the presence of T. gondii DNA, which was classified as Type I by PCR-RFLP genotyping. The sequences of 18S ribosomal RNA (18S rRNA) and 5.8S ribosomal RNA (5.8S rRNA) genes; inteRNAl transcribed spacer 1 (ITS1) and inteRNAl transcribed spacer 2 (ITS2), obtained from T. gondii isolate, have been deposited in GenBank (accession number KX459518.1). This is the first in vitro isolation and molecular identification of T. gondii from an aborted European bison foetus. The origin of this protozoan isolate indicates that the species is a significant threat to the European bison conservation program implemented in the Bialowieza Forest.
Katarzyna Goździk - One of the best experts on this subject based on the ideXlab platform.
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First Toxoplasma gondii isolate from an aborted foetus of European bison (Bison bonasus bonasus L.).
Parasitology Research, 2017Co-Authors: Bożena Moskwa, Michał K. Krzysiak, Katarzyna Goździk, Justyna Bień, Aleksandra Kornacka, Aleksandra Cybulska, Katarína Reiterová, Władysław CabajAbstract:The study was performed on a male European bison (Bison bonasus bonasus L.) foetus spontaneously aborted at the fourth or fifth month of pregnancy in the Bialowieza Forest. Serum samples from the foetus and mother revealed the presence of antibodies against T. gondii (S/P% = 88% and 75%, respectively). Mobile extracellular tachyzoites were first observed in a Vero cell culture, 110 days following inoculation of brain homogenate. PCR amplification with TGR1E1 and TGR1E2 primers confirmed the presence of T. gondii DNA, which was classified as Type I by PCR-RFLP genotyping. The sequences of 18S ribosomal RNA (18S rRNA) and 5.8S ribosomal RNA (5.8S rRNA) genes; inteRNAl transcribed spacer 1 (ITS1) and inteRNAl transcribed spacer 2 (ITS2), obtained from T. gondii isolate, have been deposited in GenBank (accession number KX459518.1). This is the first in vitro isolation and molecular identification of T. gondii from an aborted European bison foetus. The origin of this protozoan isolate indicates that the species is a significant threat to the European bison conservation program implemented in the Bialowieza Forest.
Katarína Reiterová - One of the best experts on this subject based on the ideXlab platform.
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First Toxoplasma gondii isolate from an aborted foetus of European bison (Bison bonasus bonasus L.).
Parasitology Research, 2017Co-Authors: Bożena Moskwa, Michał K. Krzysiak, Katarzyna Goździk, Justyna Bień, Aleksandra Kornacka, Aleksandra Cybulska, Katarína Reiterová, Władysław CabajAbstract:The study was performed on a male European bison (Bison bonasus bonasus L.) foetus spontaneously aborted at the fourth or fifth month of pregnancy in the Bialowieza Forest. Serum samples from the foetus and mother revealed the presence of antibodies against T. gondii (S/P% = 88% and 75%, respectively). Mobile extracellular tachyzoites were first observed in a Vero cell culture, 110 days following inoculation of brain homogenate. PCR amplification with TGR1E1 and TGR1E2 primers confirmed the presence of T. gondii DNA, which was classified as Type I by PCR-RFLP genotyping. The sequences of 18S ribosomal RNA (18S rRNA) and 5.8S ribosomal RNA (5.8S rRNA) genes; inteRNAl transcribed spacer 1 (ITS1) and inteRNAl transcribed spacer 2 (ITS2), obtained from T. gondii isolate, have been deposited in GenBank (accession number KX459518.1). This is the first in vitro isolation and molecular identification of T. gondii from an aborted European bison foetus. The origin of this protozoan isolate indicates that the species is a significant threat to the European bison conservation program implemented in the Bialowieza Forest.
