The Experts below are selected from a list of 384285 Experts worldwide ranked by ideXlab platform
J Kim - One of the best experts on this subject based on the ideXlab platform.
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thermo responsive polymer capsules in real time one step rt pcr for highly multiplex RNA Analysis
Advanced Healthcare Materials, 2020Co-Authors: J Kim, Seung Won Jung, Mi Yeon Kim, Bong Kyun Kim, Soon Hwan Kwon, Sang Kyung KimAbstract:Rapid and simple detection of RNA targets is in high demand due to the growing threat of pandemic viruses. One-step real-time, reverse transcription-polymerase chain reaction (One-step RT-qPCR) using a controlled release system of thermo-responsive materials is developed in this paper to enable high-fidelity RNA Analysis as suppressing by-products. The nanocapsules, consisting of upper critical solution temperature (UCST) material and PCR primers, carry or release the primers depending upon the temperature. The UCST nanocapsules are introduced into hydrogel microparticles incorporated with RT primers and then the target RNA is selectively amplified in the microparticle through one-step RT-qPCR. Severe side products are sharply subdued by separating the PCR primers from the RT process by means of the microparticles with nanocapsules. Because the one-step assay is now implemented in a single microparticle, multiple target RNAs can be analyzed in a simple RT-qPCR of multiple particles. Reliable 18-plex one-step RT-qPCR is successfully conducted within 30 min using single-color fluorescent optics. This work also explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the blending polymerization method. Extensible multiplex Analysis of influenza virus demonstrates the versatile uses of this one-step RT-qPCR platform.
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Thermo‐Responsive Polymer Capsules in Real‐Time One‐Step RT‐PCR for Highly Multiplex RNA Analysis
Advanced Healthcare Materials, 2020Co-Authors: J Kim, Seung Won Jung, Mi Yeon Kim, Bong Kyun Kim, Soon Hwan Kwon, Sang Kyung KimAbstract:Rapid and simple detection of RNA targets is in high demand due to the growing threat of pandemic viruses. One-step real-time, reverse transcription-polymerase chain reaction (One-step RT-qPCR) using a controlled release system of thermo-responsive materials is developed in this paper to enable high-fidelity RNA Analysis as suppressing by-products. The nanocapsules, consisting of upper critical solution temperature (UCST) material and PCR primers, carry or release the primers depending upon the temperature. The UCST nanocapsules are introduced into hydrogel microparticles incorporated with RT primers and then the target RNA is selectively amplified in the microparticle through one-step RT-qPCR. Severe side products are sharply subdued by separating the PCR primers from the RT process by means of the microparticles with nanocapsules. Because the one-step assay is now implemented in a single microparticle, multiple target RNAs can be analyzed in a simple RT-qPCR of multiple particles. Reliable 18-plex one-step RT-qPCR is successfully conducted within 30 min using single-color fluorescent optics. This work also explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the blending polymerization method. Extensible multiplex Analysis of influenza virus demonstrates the versatile uses of this one-step RT-qPCR platform.
Sang Kyung Kim - One of the best experts on this subject based on the ideXlab platform.
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thermo responsive polymer capsules in real time one step rt pcr for highly multiplex RNA Analysis
Advanced Healthcare Materials, 2020Co-Authors: J Kim, Seung Won Jung, Mi Yeon Kim, Bong Kyun Kim, Soon Hwan Kwon, Sang Kyung KimAbstract:Rapid and simple detection of RNA targets is in high demand due to the growing threat of pandemic viruses. One-step real-time, reverse transcription-polymerase chain reaction (One-step RT-qPCR) using a controlled release system of thermo-responsive materials is developed in this paper to enable high-fidelity RNA Analysis as suppressing by-products. The nanocapsules, consisting of upper critical solution temperature (UCST) material and PCR primers, carry or release the primers depending upon the temperature. The UCST nanocapsules are introduced into hydrogel microparticles incorporated with RT primers and then the target RNA is selectively amplified in the microparticle through one-step RT-qPCR. Severe side products are sharply subdued by separating the PCR primers from the RT process by means of the microparticles with nanocapsules. Because the one-step assay is now implemented in a single microparticle, multiple target RNAs can be analyzed in a simple RT-qPCR of multiple particles. Reliable 18-plex one-step RT-qPCR is successfully conducted within 30 min using single-color fluorescent optics. This work also explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the blending polymerization method. Extensible multiplex Analysis of influenza virus demonstrates the versatile uses of this one-step RT-qPCR platform.
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Thermo‐Responsive Polymer Capsules in Real‐Time One‐Step RT‐PCR for Highly Multiplex RNA Analysis
Advanced Healthcare Materials, 2020Co-Authors: J Kim, Seung Won Jung, Mi Yeon Kim, Bong Kyun Kim, Soon Hwan Kwon, Sang Kyung KimAbstract:Rapid and simple detection of RNA targets is in high demand due to the growing threat of pandemic viruses. One-step real-time, reverse transcription-polymerase chain reaction (One-step RT-qPCR) using a controlled release system of thermo-responsive materials is developed in this paper to enable high-fidelity RNA Analysis as suppressing by-products. The nanocapsules, consisting of upper critical solution temperature (UCST) material and PCR primers, carry or release the primers depending upon the temperature. The UCST nanocapsules are introduced into hydrogel microparticles incorporated with RT primers and then the target RNA is selectively amplified in the microparticle through one-step RT-qPCR. Severe side products are sharply subdued by separating the PCR primers from the RT process by means of the microparticles with nanocapsules. Because the one-step assay is now implemented in a single microparticle, multiple target RNAs can be analyzed in a simple RT-qPCR of multiple particles. Reliable 18-plex one-step RT-qPCR is successfully conducted within 30 min using single-color fluorescent optics. This work also explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the blending polymerization method. Extensible multiplex Analysis of influenza virus demonstrates the versatile uses of this one-step RT-qPCR platform.
Seung Won Jung - One of the best experts on this subject based on the ideXlab platform.
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thermo responsive polymer capsules in real time one step rt pcr for highly multiplex RNA Analysis
Advanced Healthcare Materials, 2020Co-Authors: J Kim, Seung Won Jung, Mi Yeon Kim, Bong Kyun Kim, Soon Hwan Kwon, Sang Kyung KimAbstract:Rapid and simple detection of RNA targets is in high demand due to the growing threat of pandemic viruses. One-step real-time, reverse transcription-polymerase chain reaction (One-step RT-qPCR) using a controlled release system of thermo-responsive materials is developed in this paper to enable high-fidelity RNA Analysis as suppressing by-products. The nanocapsules, consisting of upper critical solution temperature (UCST) material and PCR primers, carry or release the primers depending upon the temperature. The UCST nanocapsules are introduced into hydrogel microparticles incorporated with RT primers and then the target RNA is selectively amplified in the microparticle through one-step RT-qPCR. Severe side products are sharply subdued by separating the PCR primers from the RT process by means of the microparticles with nanocapsules. Because the one-step assay is now implemented in a single microparticle, multiple target RNAs can be analyzed in a simple RT-qPCR of multiple particles. Reliable 18-plex one-step RT-qPCR is successfully conducted within 30 min using single-color fluorescent optics. This work also explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the blending polymerization method. Extensible multiplex Analysis of influenza virus demonstrates the versatile uses of this one-step RT-qPCR platform.
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Thermo‐Responsive Polymer Capsules in Real‐Time One‐Step RT‐PCR for Highly Multiplex RNA Analysis
Advanced Healthcare Materials, 2020Co-Authors: J Kim, Seung Won Jung, Mi Yeon Kim, Bong Kyun Kim, Soon Hwan Kwon, Sang Kyung KimAbstract:Rapid and simple detection of RNA targets is in high demand due to the growing threat of pandemic viruses. One-step real-time, reverse transcription-polymerase chain reaction (One-step RT-qPCR) using a controlled release system of thermo-responsive materials is developed in this paper to enable high-fidelity RNA Analysis as suppressing by-products. The nanocapsules, consisting of upper critical solution temperature (UCST) material and PCR primers, carry or release the primers depending upon the temperature. The UCST nanocapsules are introduced into hydrogel microparticles incorporated with RT primers and then the target RNA is selectively amplified in the microparticle through one-step RT-qPCR. Severe side products are sharply subdued by separating the PCR primers from the RT process by means of the microparticles with nanocapsules. Because the one-step assay is now implemented in a single microparticle, multiple target RNAs can be analyzed in a simple RT-qPCR of multiple particles. Reliable 18-plex one-step RT-qPCR is successfully conducted within 30 min using single-color fluorescent optics. This work also explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the blending polymerization method. Extensible multiplex Analysis of influenza virus demonstrates the versatile uses of this one-step RT-qPCR platform.
Mi Yeon Kim - One of the best experts on this subject based on the ideXlab platform.
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thermo responsive polymer capsules in real time one step rt pcr for highly multiplex RNA Analysis
Advanced Healthcare Materials, 2020Co-Authors: J Kim, Seung Won Jung, Mi Yeon Kim, Bong Kyun Kim, Soon Hwan Kwon, Sang Kyung KimAbstract:Rapid and simple detection of RNA targets is in high demand due to the growing threat of pandemic viruses. One-step real-time, reverse transcription-polymerase chain reaction (One-step RT-qPCR) using a controlled release system of thermo-responsive materials is developed in this paper to enable high-fidelity RNA Analysis as suppressing by-products. The nanocapsules, consisting of upper critical solution temperature (UCST) material and PCR primers, carry or release the primers depending upon the temperature. The UCST nanocapsules are introduced into hydrogel microparticles incorporated with RT primers and then the target RNA is selectively amplified in the microparticle through one-step RT-qPCR. Severe side products are sharply subdued by separating the PCR primers from the RT process by means of the microparticles with nanocapsules. Because the one-step assay is now implemented in a single microparticle, multiple target RNAs can be analyzed in a simple RT-qPCR of multiple particles. Reliable 18-plex one-step RT-qPCR is successfully conducted within 30 min using single-color fluorescent optics. This work also explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the blending polymerization method. Extensible multiplex Analysis of influenza virus demonstrates the versatile uses of this one-step RT-qPCR platform.
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Thermo‐Responsive Polymer Capsules in Real‐Time One‐Step RT‐PCR for Highly Multiplex RNA Analysis
Advanced Healthcare Materials, 2020Co-Authors: J Kim, Seung Won Jung, Mi Yeon Kim, Bong Kyun Kim, Soon Hwan Kwon, Sang Kyung KimAbstract:Rapid and simple detection of RNA targets is in high demand due to the growing threat of pandemic viruses. One-step real-time, reverse transcription-polymerase chain reaction (One-step RT-qPCR) using a controlled release system of thermo-responsive materials is developed in this paper to enable high-fidelity RNA Analysis as suppressing by-products. The nanocapsules, consisting of upper critical solution temperature (UCST) material and PCR primers, carry or release the primers depending upon the temperature. The UCST nanocapsules are introduced into hydrogel microparticles incorporated with RT primers and then the target RNA is selectively amplified in the microparticle through one-step RT-qPCR. Severe side products are sharply subdued by separating the PCR primers from the RT process by means of the microparticles with nanocapsules. Because the one-step assay is now implemented in a single microparticle, multiple target RNAs can be analyzed in a simple RT-qPCR of multiple particles. Reliable 18-plex one-step RT-qPCR is successfully conducted within 30 min using single-color fluorescent optics. This work also explains the facile fabrication processes used for the thermo-responsive nanocapsules and hydrogel microparticles by the blending polymerization method. Extensible multiplex Analysis of influenza virus demonstrates the versatile uses of this one-step RT-qPCR platform.
David Issadore - One of the best experts on this subject based on the ideXlab platform.
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a magnetic micropore chip for rapid 1 hour unbiased circulating tumor cell isolation and in situ RNA Analysis
Lab on a Chip, 2017Co-Authors: Neha Bhagwat, Stephanie S Yee, Taylor A Black, Colleen Redlinger, Janae Romeo, Mark H Ohara, Arjun Raj, Erica L Carpenter, Ben Z Stanger, David IssadoreAbstract:The use of microtechnology for the highly selective isolation and sensitive detection of circulating tumor cells has shown enormous promise. One challenge for this technology is that the small feature sizes – which are the key to this technology's performance – can result in low sample throughput and susceptibility to clogging. Additionally, conventional molecular Analysis of CTCs often requires cells to be taken off-chip for sample preparation and purification before Analysis, leading to the loss of rare cells. To address these challenges, we have developed a microchip platform that combines fast, magnetic micropore based negative immunomagnetic selection (>10 mL h−1) with rapid on-chip in situ RNA profiling (>100× faster than conventional RNA labeling). This integrated chip can isolate both rare circulating cells and cell clusters directly from whole blood and allow individual cells to be profiled for multiple RNA cancer biomarkers, achieving sample-to-answer in less than 1 hour for 10 mL of whole blood. To demonstrate the power of this approach, we applied our device to the circulating tumor cell based diagnosis of pancreatic cancer. We used a genetically engineered lineage-labeled mouse model of pancreatic cancer (KPCY) to validate the performance of our chip. We show that in a cohort of patient samples (N = 25) that this device can detect and perform in situ RNA Analysis on circulating tumor cells in patients with pancreatic cancer, even in those with extremely sparse CTCs (<1 CTC mL−1 of whole blood).
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Abstract 386: Rapid in situ RNA Analysis of circulating tumor cells using magnetic micropore-based sorting and Turbo FISH
Tumor Biology, 2015Co-Authors: Neha Bhagwat, Stephanie S Yee, Erica L Carpenter, Ben Z Stanger, David IssadoreAbstract:Pancreatic cancer is the fourth most common cause of cancer-related death in the US, with a five year survival rate of only 6%. Recently, we have shown that circulating pancreatic cells can be detected at the onset of the disease cycle (pre-Stage 1). Thus, there is both a great need for early diagnosis and an opportunity, due to the early presence of circulating tumor cells (CTCs). Currently, the gold standard for CTC detection cannot resolve these extremely sparse, heterogeneous CTCs, which do not have any single surface marker that allows them to be targeted. Additionally, conventional downstream analyses are limited to either protein expression of individual cells on-chip, which has limited sensitivity to relevant biomarkers, or genetic testing off-chip, which has limited practical clinical use. To address these challenges, we have developed a rapid ( A key innovation is the development of our magnetic micropore based sorting technique, which preserves the low cell-loss and specificity of microfluidic sorting but at >10x greater flow rates and an insensitivity to clogging, enabling use directly on whole blood. The TEMPO filter consists of an electroplated 1.25 μm NiFe, 30 μm pores, and 5.1×10E5 pores total. Using this chip, we have demonstrated extremely high sensitivity to rare cells (LOD ∼ 1 cell / 7.5 mL blood) and exponentially improved sorting efficiency by stacking multiple TEMPO filters in series. Furthermore, single molecules of targeted RNA in each individual CTC could be resolved. Citation Format: Jin A Ko, Neha Bhagwat, Stephanie S. Yee, Erica Carpenter, Ben Stanger, David Issadore. Rapid in situ RNA Analysis of circulating tumor cells using magnetic micropore-based sorting and Turbo FISH. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 386. doi:10.1158/1538-7445.AM2015-386
