The Experts below are selected from a list of 141 Experts worldwide ranked by ideXlab platform
Patrick J. Hrdlicka - One of the best experts on this subject based on the ideXlab platform.
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c2 pyrene functionalized triazole linked dna universal dna RNA Hybridization probes
Journal of Organic Chemistry, 2012Co-Authors: Sujay P. Sau, Patrick J. HrdlickaAbstract:Development of universal Hybridization probes, that is, oligonucleotides displaying identical affinity toward matched and mismatched DNA/RNA targets, has been a longstanding goal due to potential applications as degenerate PCR primers and microarray probes. The classic approach toward this end has been the use of “universal bases” that either are based on hydrogen-bonding purine derivatives or aromatic base analogues without hydrogen-bonding capabilities. However, development of probes that result in truly universal Hybridization without compromising duplex thermostability has proven challenging. Here we have used the “click reaction” to synthesize four C2′-pyrene-functionalized triazole-linked 2′-deoxyuridine phosphoramidites. We demonstrate that oligodeoxyribonucleotides modified with the corresponding monomers display (a) minimally decreased thermal affinity toward DNA/RNA complements relative to reference strands, (b) highly robust universal Hybridization characteristics (average differences in therma...
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C2'-pyrene-functionalized triazole-linked DNA: universal DNA/RNA Hybridization probes.
The Journal of organic chemistry, 2011Co-Authors: Sujay P. Sau, Patrick J. HrdlickaAbstract:Development of universal Hybridization probes, that is, oligonucleotides displaying identical affinity toward matched and mismatched DNA/RNA targets, has been a longstanding goal due to potential applications as degenerate PCR primers and microarray probes. The classic approach toward this end has been the use of “universal bases” that either are based on hydrogen-bonding purine derivatives or aromatic base analogues without hydrogen-bonding capabilities. However, development of probes that result in truly universal Hybridization without compromising duplex thermostability has proven challenging. Here we have used the “click reaction” to synthesize four C2′-pyrene-functionalized triazole-linked 2′-deoxyuridine phosphoramidites. We demonstrate that oligodeoxyribonucleotides modified with the corresponding monomers display (a) minimally decreased thermal affinity toward DNA/RNA complements relative to reference strands, (b) highly robust universal Hybridization characteristics (average differences in therma...
Sujay P. Sau - One of the best experts on this subject based on the ideXlab platform.
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c2 pyrene functionalized triazole linked dna universal dna RNA Hybridization probes
Journal of Organic Chemistry, 2012Co-Authors: Sujay P. Sau, Patrick J. HrdlickaAbstract:Development of universal Hybridization probes, that is, oligonucleotides displaying identical affinity toward matched and mismatched DNA/RNA targets, has been a longstanding goal due to potential applications as degenerate PCR primers and microarray probes. The classic approach toward this end has been the use of “universal bases” that either are based on hydrogen-bonding purine derivatives or aromatic base analogues without hydrogen-bonding capabilities. However, development of probes that result in truly universal Hybridization without compromising duplex thermostability has proven challenging. Here we have used the “click reaction” to synthesize four C2′-pyrene-functionalized triazole-linked 2′-deoxyuridine phosphoramidites. We demonstrate that oligodeoxyribonucleotides modified with the corresponding monomers display (a) minimally decreased thermal affinity toward DNA/RNA complements relative to reference strands, (b) highly robust universal Hybridization characteristics (average differences in therma...
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C2'-pyrene-functionalized triazole-linked DNA: universal DNA/RNA Hybridization probes.
The Journal of organic chemistry, 2011Co-Authors: Sujay P. Sau, Patrick J. HrdlickaAbstract:Development of universal Hybridization probes, that is, oligonucleotides displaying identical affinity toward matched and mismatched DNA/RNA targets, has been a longstanding goal due to potential applications as degenerate PCR primers and microarray probes. The classic approach toward this end has been the use of “universal bases” that either are based on hydrogen-bonding purine derivatives or aromatic base analogues without hydrogen-bonding capabilities. However, development of probes that result in truly universal Hybridization without compromising duplex thermostability has proven challenging. Here we have used the “click reaction” to synthesize four C2′-pyrene-functionalized triazole-linked 2′-deoxyuridine phosphoramidites. We demonstrate that oligodeoxyribonucleotides modified with the corresponding monomers display (a) minimally decreased thermal affinity toward DNA/RNA complements relative to reference strands, (b) highly robust universal Hybridization characteristics (average differences in therma...
Timothy M. Block - One of the best experts on this subject based on the ideXlab platform.
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In situ DNA PCR and RNA Hybridization detection of herpes simplex virus sequences in trigeminal gangliaof latently infected mice
Virology, 1995Co-Authors: Anand Mehta, John Maggioncalda, Omar Bagasra, Seshamma Thikkavarapu, Pamujula Saikumari, Tibor Valyi-nagy, Nigel W. Fraser, Timothy M. BlockAbstract:The presence of herpes simplex virus (HSV-1) DNA in the trigeminal ganglia of latently infected mice was detected byan in situ DNA polymerase chain reaction (PCR), which includes a DNA:DNA Hybridization step (indirect in situ PCR). These results were compared to the number of neurons possessing the HSV-1 latency associated transcript (LAT), as detected by in situ RNA Hybridization with LAT probes. Sensitivity assays were shown to detect a single copy cellular gene in 48% of neuronal cell bodies. The results suggest that in situ PCR is an effective method to locate and detect HSV-1 within latently infected neurons. Moreover, the number of neurons found to be harboring HSV-1, by the method of in situ PCR, which does not depend upon virus gene expression, is within threefold of the number detected by in situ Hybridization for LAT. Therefore, this report describes the first detection of HSV-1 DNA in latently infected murine trigeminal ganglia by the method of indirect in situ PCR, and compares the findings to the number of neurons expressing LAT, as assessed by conventional in situ Hybridization.
Anand Mehta - One of the best experts on this subject based on the ideXlab platform.
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In situ DNA PCR and RNA Hybridization detection of herpes simplex virus sequences in trigeminal gangliaof latently infected mice
Virology, 1995Co-Authors: Anand Mehta, John Maggioncalda, Omar Bagasra, Seshamma Thikkavarapu, Pamujula Saikumari, Tibor Valyi-nagy, Nigel W. Fraser, Timothy M. BlockAbstract:The presence of herpes simplex virus (HSV-1) DNA in the trigeminal ganglia of latently infected mice was detected byan in situ DNA polymerase chain reaction (PCR), which includes a DNA:DNA Hybridization step (indirect in situ PCR). These results were compared to the number of neurons possessing the HSV-1 latency associated transcript (LAT), as detected by in situ RNA Hybridization with LAT probes. Sensitivity assays were shown to detect a single copy cellular gene in 48% of neuronal cell bodies. The results suggest that in situ PCR is an effective method to locate and detect HSV-1 within latently infected neurons. Moreover, the number of neurons found to be harboring HSV-1, by the method of in situ PCR, which does not depend upon virus gene expression, is within threefold of the number detected by in situ Hybridization for LAT. Therefore, this report describes the first detection of HSV-1 DNA in latently infected murine trigeminal ganglia by the method of indirect in situ PCR, and compares the findings to the number of neurons expressing LAT, as assessed by conventional in situ Hybridization.
J. R. Gentsch - One of the best experts on this subject based on the ideXlab platform.
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Characterization of human rotavirus genotype P[8]G5 from Brazil by probe-Hybridization and sequence
Archives of Virology, 1996Co-Authors: A. A. Alfieri, O. Nakagomi, J. P. G. Leite, E. Kaga, P. A. Woods, R. I. Glass, J. R. GentschAbstract:We report the molecular characterization of rotavirus genotype P[8]G5 strains found in fecal specimens collected in four different regions of Brazil, using digoxigenin (dig)-labeled oligonucleotide probes, sequence analysis, and RNA-RNA Hybridization. The closest sequence relationships of the neutralization antigens of these strains were to the VP4 protein of P1A[8]G1 strain KU (93.3% identity in amino acids 11 to 282) and to the VP7 protein of G serotype 5 strain OSU (87.6% identity in amino acids 8 to 232). Based on VP7 sequence differences, we designed dig-probes that allowed us to discriminate porcine OSU-like strains from G5 strains isolated from Brazilian infants. The genetic relationships of two P[8]G5 isolates to other rotavirus genogroups were analyzed by RNA-RNA Hybridization with [^32P]-GTP probes representative of serotypes P1A[8]G1 (Wa), P[8]G3 (AU17), and P9[7]G5 (OSU). The Brazilian P[8]G5 strains showed sequence homology with genes of Wa-like and OSU-like strains, suggesting that these two strains were naturally occurring reassortants between members of the Wa and porcine rotavirus genogroups. The identification of these strains in diverse geographic areas of Brazil underscores their stability and demonstrates the emergence of clinically important rotavirus diarrhea strains by reassortment.
