The Experts below are selected from a list of 72 Experts worldwide ranked by ideXlab platform
Goran Akusjarvi - One of the best experts on this subject based on the ideXlab platform.
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control of adenovirus alteRNAtive RNA splicing effect of viral dna replication on RNA Splice Site choice
Gene, 1991Co-Authors: Sten Larsson, Janpeter Kreivi, Goran AkusjarviAbstract:Abstract The primary transcripts of most adenovirus transcription units are processed into multiple, alteRNAtively Spliced mRNAs. The relative concentrations of such differentially processed mRNAs changes during the infectious cycle. The factors that control this temporal shift in mRNA abundance have not yet been characterized. In the experiments presented here we have examined mRNA synthesis from three viral transcription units: two early regions E 1 a and E 1 b , and late region L1 . We show that viral DNA replication plays a key role in the control of cytoplasmic mRNA expression from these regions. In the absence of efficient late protein synthesis, viral DNA replication was sufficient to induce a substantial fraction of the E 1 a , E 1 b and L1 transcripts to shift from the early to the late pattern of mRNA structure. The shift was not complete under the conditions used, suggesting that viral proteins, although not essential for the process, play an important regulatory role. The requirement for late viral protein synthesis differed between the three transcription units examined. This dependence was most pronounced for correct L1 mRNA production. Viral DNA replication was sufficient to trigger a significant shift in L1 alteRNAtive 3 ' Splice Site selection. However, in the absence of late translation the L1 pre-mRNA was aberrantly Spliced.
Sten Larsson - One of the best experts on this subject based on the ideXlab platform.
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control of adenovirus alteRNAtive RNA splicing effect of viral dna replication on RNA Splice Site choice
Gene, 1991Co-Authors: Sten Larsson, Janpeter Kreivi, Goran AkusjarviAbstract:Abstract The primary transcripts of most adenovirus transcription units are processed into multiple, alteRNAtively Spliced mRNAs. The relative concentrations of such differentially processed mRNAs changes during the infectious cycle. The factors that control this temporal shift in mRNA abundance have not yet been characterized. In the experiments presented here we have examined mRNA synthesis from three viral transcription units: two early regions E 1 a and E 1 b , and late region L1 . We show that viral DNA replication plays a key role in the control of cytoplasmic mRNA expression from these regions. In the absence of efficient late protein synthesis, viral DNA replication was sufficient to induce a substantial fraction of the E 1 a , E 1 b and L1 transcripts to shift from the early to the late pattern of mRNA structure. The shift was not complete under the conditions used, suggesting that viral proteins, although not essential for the process, play an important regulatory role. The requirement for late viral protein synthesis differed between the three transcription units examined. This dependence was most pronounced for correct L1 mRNA production. Viral DNA replication was sufficient to trigger a significant shift in L1 alteRNAtive 3 ' Splice Site selection. However, in the absence of late translation the L1 pre-mRNA was aberrantly Spliced.
Bert W Omalley - One of the best experts on this subject based on the ideXlab platform.
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differential recruitment of nuclear receptor coactivators may determine alteRNAtive RNA Splice Site choice in target genes
Proceedings of the National Academy of Sciences of the United States of America, 2004Co-Authors: Didier Auboeuf, Dennis H Dowhan, Yun Kyoung Kang, Kimberly Larkin, Susan M Berget, Bert W OmalleyAbstract:The biological consequences of steroid hormone-mediated transcriptional activation of target genes might be difficult to predict because alteRNAtive splicing of a single neosynthesized precursor RNA can result in production of different protein isoforms with oppoSite biological activities. Therefore, an important question to address is the manner in which steroid hormones affect the splicing of their target gene transcripts. In this report, we demonstrate that individual steroid hormones had different and oppoSite effects on alteRNAtive splicing decisions, stimulating the production of different Spliced variants produced from genes driven by steroid hormone-dependent promoters. Steroid hormone transcriptional effects are mediated by steroid hormone receptor coregulators that also modify alteRNAtive splicing decisions. Our data suggest that activated steroid hormone receptors recruit coregulators to the target promoter that participate in both the production and the splicing of the target gene transcripts. Because different coregulators activating transcription can have oppoSite effects on alteRNAtive splicing decisions, we conclude that the precise nature of the transcriptional coregulators recruited by activated steroid receptors, depending on the promoter and cellular contexts, may play a major role in regulating the nature of the Spliced variants produced from certain target genes in response to steroid hormones.
Janpeter Kreivi - One of the best experts on this subject based on the ideXlab platform.
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control of adenovirus alteRNAtive RNA splicing effect of viral dna replication on RNA Splice Site choice
Gene, 1991Co-Authors: Sten Larsson, Janpeter Kreivi, Goran AkusjarviAbstract:Abstract The primary transcripts of most adenovirus transcription units are processed into multiple, alteRNAtively Spliced mRNAs. The relative concentrations of such differentially processed mRNAs changes during the infectious cycle. The factors that control this temporal shift in mRNA abundance have not yet been characterized. In the experiments presented here we have examined mRNA synthesis from three viral transcription units: two early regions E 1 a and E 1 b , and late region L1 . We show that viral DNA replication plays a key role in the control of cytoplasmic mRNA expression from these regions. In the absence of efficient late protein synthesis, viral DNA replication was sufficient to induce a substantial fraction of the E 1 a , E 1 b and L1 transcripts to shift from the early to the late pattern of mRNA structure. The shift was not complete under the conditions used, suggesting that viral proteins, although not essential for the process, play an important regulatory role. The requirement for late viral protein synthesis differed between the three transcription units examined. This dependence was most pronounced for correct L1 mRNA production. Viral DNA replication was sufficient to trigger a significant shift in L1 alteRNAtive 3 ' Splice Site selection. However, in the absence of late translation the L1 pre-mRNA was aberrantly Spliced.
K. Whaley - One of the best experts on this subject based on the ideXlab platform.
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An RNA Splice Site mutation in the C1-inhibitor gene causes type I hereditary angio-oedema
Human Genetics, 1991Co-Authors: Z. Siddique, A. R. Mcphaden, D. F. Lappin, K. WhaleyAbstract:Restriction fragment length polymorphism analysis, the polymerase chain reaction and nucleotide sequencing have been used to characterise a single base substitution (G→T) at nucleotide 8863 in the C1-inhibitor gene. This destroys the 5′ donor Splice Site recognition motif of the sixth intron. Family studies suggest that the mutation is responsible for type I hereditary angio-oedema in a studied kindred.
