The Experts below are selected from a list of 2259 Experts worldwide ranked by ideXlab platform
W. D. Nafarnda - One of the best experts on this subject based on the ideXlab platform.
-
The Application of Erythrocyte Rosette Test to Characterize T-Like Lymphocytes in the Mud Catfish (Clarias gariepinus)
2011Co-Authors: W. D. NafarndaAbstract:55 ABSTRACT The Erythrocyte Rosette Test, a technique used to characterize T-lymphocytes using sheep red blood cells was evaluated to demonstrate T-like lymphocytes sub-population in the peripheral blood of the mud catfish (Clarias gariepinus). Forty eight young adult mud catfish weighing between 100-150g each were used in the study. The effects of thymectomy and injected anti lymphocyte serum on the degree of erythrocyte Rosette formation were assessed. Rosette of sheep red blood cells around the mud catfish lymphocytes were observed in all experimental groups. The numbers of Rosette formed were significantly higher (p
-
the application of erythrocyte Rosette Test to characterize t like lymphocytes in the mud catfish clarias gariepinus
2011Co-Authors: W. D. Nafarnda, S M KaweAbstract:55 ABSTRACT The Erythrocyte Rosette Test, a technique used to characterize T-lymphocytes using sheep red blood cells was evaluated to demonstrate T-like lymphocytes sub-population in the peripheral blood of the mud catfish (Clarias gariepinus). Forty eight young adult mud catfish weighing between 100-150g each were used in the study. The effects of thymectomy and injected anti lymphocyte serum on the degree of erythrocyte Rosette formation were assessed. Rosette of sheep red blood cells around the mud catfish lymphocytes were observed in all experimental groups. The numbers of Rosette formed were significantly higher (p<0.05) in the untreated group as compared to the treated group.
Sun Bing-jun - One of the best experts on this subject based on the ideXlab platform.
-
Determination of the Immune Activity of Sheep Placenta Extracts by Erythrocyte Rosette Test
Journal of Anhui Agricultural Sciences, 2012Co-Authors: Sun Bing-junAbstract:The immune activity and the best active concentration of sheep placenta extract were assayed by erythrocyte Rosette Test.Three concentration gradients were set up to use erythrocyte Rosette method to assay the optimal concentrations of thymus lymphocytes and sheep red blood cells.The lymphocytes were put into the 45 ℃ water bath for 45min to destroy its receptor,and then compared with the lymphocytes without de-receptor by determining the concentration of sheep placenta extract by erythrocyte Rosette Test.Meantime,four concentration gradients of thymus lymphocytes were set up to assay the concentration when the immune activity of sheep placenta extract is the highest.The results showed that the sheep placenta extracts had high immune activity and it reached the highest when the cell concentration of basic solution was 10-2 /ml.
Yang Lin - One of the best experts on this subject based on the ideXlab platform.
-
Study on the Influential factors of T Lymphocyte-E-Rosette Test Used to Examine the Biological Activity of Thymosin
Pharmaceutical biotechnology, 2007Co-Authors: Yang LinAbstract:To improve the conditions of T lymphocyte-E-Rosette Test for examining the biological activity of thymosin,the effects of different doffed E receptor methods,the ratio of lymphocytes to sheep red blood cells,the pH value of Hank's solution,and the centrifugal speed on the E-rose formation were compared and the dyeing or counting time that influence the observation in the E-rose formation was studied.The E-rose formed from different sources of lymphocytes and different red blood cells was also observed.It was found that the optimied doffed E method could reflect the biological activity of thymosin,and the pH value of Hank's solution between 7.0～7.5 was the best result of rose formation.The best ratio of lymphocytes to sheep red blood cells was 1∶20,the centrifugal speed should be set at 800r/min,the sample should be put at 4℃ after dying,and it should be observed in 16～20h.The thymosin of guinea pig and the rabbit red blood cell,the thymosin of calf and sheep red blood cell both have the pertinency to form E-rose.The improved conditions can increase the accuracy rate when examinind the biological activity of thymosin by T lymphocyte-E-Rosette Test.
Wang Yun-ji - One of the best experts on this subject based on the ideXlab platform.
-
Immunocompetence of aqueous extract for three kinds of Chinese traditional medicines detected by E-Rosette Test
Journal of Dalian Institute of Light Industry, 2005Co-Authors: Wang Yun-jiAbstract:This paper reports the immunocompetence research of the aqueous extracts from earthworm, Scutellaria Baicalensys, and taraxacum detected respectively by E-Rosette Test. It reveals that the aqueous extract of earthworm exhibits the best immunocompetence. The E-Rosette concentration approaches 19% in average with 200?μg/mL of earthworm extract in concentration, 13% in average with 20?μg/mL of taraxacum extract, and 7% in average with 40?μg/mL of Scutellaria Baicalensys extract.
S M Kawe - One of the best experts on this subject based on the ideXlab platform.
-
the application of erythrocyte Rosette Test to characterize t like lymphocytes in the mud catfish clarias gariepinus
2011Co-Authors: W. D. Nafarnda, S M KaweAbstract:55 ABSTRACT The Erythrocyte Rosette Test, a technique used to characterize T-lymphocytes using sheep red blood cells was evaluated to demonstrate T-like lymphocytes sub-population in the peripheral blood of the mud catfish (Clarias gariepinus). Forty eight young adult mud catfish weighing between 100-150g each were used in the study. The effects of thymectomy and injected anti lymphocyte serum on the degree of erythrocyte Rosette formation were assessed. Rosette of sheep red blood cells around the mud catfish lymphocytes were observed in all experimental groups. The numbers of Rosette formed were significantly higher (p<0.05) in the untreated group as compared to the treated group.
