The Experts below are selected from a list of 15 Experts worldwide ranked by ideXlab platform
The Betty Smithers Design Collection At Staffordshire University - One of the best experts on this subject based on the ideXlab platform.
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Dirt Devil Handy 150 Vacuum Cleaner
Staffordshire University, 2010Co-Authors: Staffordshire University, The Betty Smithers Design Collection At Staffordshire UniversityAbstract:Hand-held vacuum cleaner with red plastic body and handle and balck and red fabric dust bag. Black Rubber Bung to front for attachments. Silver chrome trim around roller and brushes to front underside. Siver label with 'Dirt Devil' and logo in black. Black plastic rocker switch to top. Complete in original box (damaged) with instruction leaflets. Maker: Royal. Date: 1991 (circa) - from the The Betty Smithers Design Collection at Staffordshire University.
Sharon A. Tooze - One of the best experts on this subject based on the ideXlab platform.
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chapter 11 subcellular fractionation procedures and metabolic labeling using 35s sulfate to isolate dense core secretory granules from neuroendocrine cell lines
Cell Biology (Third Edition)#R##N#A Laboratory Handbook, 2006Co-Authors: Sharon A. ToozeAbstract:Publisher Summary This chapter describes subcellular fractionation procedures and metabolic labeling using sulfate to isolate dense core secretory granules from neuroendocrine cell lines. A combination of velocity-controlled differential and equilibrium density centrifugation achieved a separation of immature secretory granules and the trans-Golgi network (TGN). A cell scraper is made from a silicon Rubber Bung by cutting the Bung with a single-sided razor blade first horizontally across the widest part and then vertically in half. The tip of a plastic 10-ml pipette is then inserted into the center of the cut Bung. The centrifugation procedure can be checked by assaying for TGN, secretory granule, or other compartment-specific markers in each fraction from the velocity and equilibrium gradients. This can be done by Western blotting using antibodies to the marker proteins or by metabolic labeling. Protein tyrosine sulfation is a posttranslational modification found in some secretory proteins, including secretogranin II (SgII) and an unidentified constitutively secreted heparan sulphated proteoglycan (HSPG). The enzyme tyrosylprotein sulfotransferase responsible for sulfation is a resident TGN protein.
Staffordshire University - One of the best experts on this subject based on the ideXlab platform.
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Dirt Devil Handy 150 Vacuum Cleaner
Staffordshire University, 2010Co-Authors: Staffordshire University, The Betty Smithers Design Collection At Staffordshire UniversityAbstract:Hand-held vacuum cleaner with red plastic body and handle and balck and red fabric dust bag. Black Rubber Bung to front for attachments. Silver chrome trim around roller and brushes to front underside. Siver label with 'Dirt Devil' and logo in black. Black plastic rocker switch to top. Complete in original box (damaged) with instruction leaflets. Maker: Royal. Date: 1991 (circa) - from the The Betty Smithers Design Collection at Staffordshire University.
N Nayak - One of the best experts on this subject based on the ideXlab platform.
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Fungal infections of the eye- laboratory diagnosis and treatment
2014Co-Authors: N NayakAbstract:Infections of the eye give rise to severe ocular morbidity and blindness include keratitis, orbital cellulites, endophthalmitis and dacryocystitis. Corneal blindness, in developing countries is predominantly associated with infections. In India, nearly 30-35 % of all culture positive infectious keratitis are caused fungi. Laboratory diagnosis mainly depends upon proper collection and transport of clinical specimens. In fungal keratitis, corneal scraping is the ideal sample, but occasionally corneal biopsy or anterior chamber aspirate may also be needed. Corneal scraping is usually by Kimura spatula, under a slit lamp examination, after anaesthetizing the cornea with topical anaesthetic like 0.4 % proparcaine. Corneal biopsy is done by a minor trephining and AC aspirate using a sterile tuberculin syringe. In case of endophthalmitis, 150-200ìl of aqueous humour is collected. Vitreous fluid (500-1000 ìl), however, is collected by pars plana vitrectomy onto sterile tuberculin syringe, the needle is then fixed to a sterile Rubber Bung after expelling air from the syringe. The collected sample is immediately transported to the laboratory. Swabs from the regurgitating lacrimnal sacs and wound aspirate/ swabs are the ideal specimens for dacryocystitis and orbital cellulites, respectively. These samples are cultured onto SDA slants following standard procedures. The main draw back of culture is its long incubation time (5 to 14 days), though it is indispensable from the view point of the specificity. Direct examination (KOH we
Stacey Sonya - One of the best experts on this subject based on the ideXlab platform.
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BD PhaSeal™ and particulate contamination
'Wiley', 2018Co-Authors: Smith, Evonne Katherine, Sharma Rajinder, Stacey SonyaAbstract:Background: The closed system transfer device, BD PhaSeal™, is used to compound individual doses of cytotoxic medication in the Aseptic Production Unit (APU) of the Lady Cilento Children's Hospital (LCCH) Brisbane, Queensland, Australia. The APU noted a large number of cytotoxic medications compounded using BD PhaSeal™ required remaking of the product due to visible particulate contamination. The small gray particulate contaminate was assumed to be Rubber Bung produced by ‘coring’ the Rubber stopper of the drug vial when using the BD PhaSeal™ Protector P50 as part of the compounding process. Aim: The aim of this study was to determine if substituting the BD PhaSeal™ Protector P50 with the BD PhaSeal™ Protector P55 reduced the level of particulate contamination in cytotoxic compounded products. Method: Phase 1 – baseline collection of contamination over 4 months. Phase 2 – substitute the Protector P50 with the P55 into the compounding process to compound a single medicine product, vincristine in sodium chloride 0.9% 50 mL bags for 2 weeks. Phase 3 – substitute the Protector P50 with the P55 into the compounding process of all cytotoxic medicines for 4 months. Results: Phase 1 – 134 products were contaminated out of 2501 compounded products (5.3%). Phase 2 – one out of 75 vincristine products had particulate contamination (1.3%) using P55. Phase 3 – 28 contaminated products out of 3877 (0.72%) using P55. Conclusion: This study demonstrated that substituting BD PhaSeal™ Protector P50 with the Protector P55 reduced the incidence of particulate contamination of cytotoxic medicines from 5.3–0.72% of products. This provides significant resource and financial benefits
