The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Asmita V Patel - One of the best experts on this subject based on the ideXlab platform.
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A Straight-Chain Alcohol Glycoside, with Smooth Muscle Relaxant Activity, from Rubus idaeus (Raspberry) Leaves:
Natural Product Communications, 2007Co-Authors: Asmita V Patel, Christopher G Dacke, Gerald Blunden, J. Rojas-veraAbstract:A methanolic extract of Rubus idaeus leaves, when fractionated by column chromatography, yielded two distinct bands containing compounds that demonstrated in vitro, dose dependent, smooth muscle re...
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Smooth muscle relaxant triterpenoid glycosides from Rubus idaeus (raspberry) leaves.
Natural Product Communications, 2006Co-Authors: Janne Rojas. Vera, Christopher G Dacke, Gerald Blunden, Asmita V PatelAbstract:A methanol ext. of Rubus idaeus leaves had a relaxant activity on transmurally stimulated Guinea pig ileum prepns. in vitro. The ext., when fractionated by column chromatog., yielded 2 distinct bands with pharmacol. activity. After extensive column and preparative TLC sepn., several compds. with smooth muscle relaxant properties were obtained. From band 2, 3 triterpenoid glycosides were characterized, which had significant muscle relaxant activity. From spectroscopic and spectrometric data, these were identified as 3β-(O-β-D-glucopyranosyl)-olean-12-ene-1α, 2α, 3β-triol (TG1); 28-(O-β-D-glucopyranosyl)-urs-12-ene-2α, 3β, 19α-trihydroxy-28-oic acid (TG2); and 3β-(O-β-D-glucopyranosyl)-olean-12-ene-1α, 2α, 3β-trihydroxy-28-oic acid (TG3). To the best of the authors' knowledge, TG1 and TG3 are novel compds. [on SciFinder(R)]
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Triterpenoids, Including One with Smooth Muscle Relaxant Activity, from Rubus idaeus (Raspberry) Leaves:
Natural Product Communications, 2006Co-Authors: J. Rojas-vera, Christopher G Dacke, Asmita V Patel, Gerald BlundenAbstract:A methanol extract of Rubus idaeus leaves was fractionated by column chromatography and the eluted samples were tested for their ability to relax transmurally stimulated Guinea pig ileum preparatio...
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relaxant activity of raspberry Rubus idaeus leaf extract in guinea pig ileum in vitro
Phytotherapy Research, 2002Co-Authors: Janne Rojasvera, Asmita V Patel, C. G. DackeAbstract:Tea made from the leaves of Rubus idaeus L. (raspberry) has been used for centuries as a folk medicine to treat wounds, diarrhoea, colic pain and as a uterine relaxant. Extracts of dried raspberry leaves prepared with different solvents, (n-hexane, ethyl acetate, chloroform and methanol) were tested in vitro for relaxant activity on transmurally stimulated guinea-pig ileum. The methanol (MeOH) extract exhibited the largest response and also indicated that the active compounds are of a relatively polar nature. Hence the bulk of the leaves were extracted with methanol and the dried extract fractionated on a silica gel column, eluting with chloroform, mixtures of chloroform and methanol and finally methanol. Each fraction was examined by thin layer chromatography and tested for relaxant activity in an in vitro transmurally stimulated guinea-pig ileum preparation. The fractions eluted with chloroform (CHCl3) lacked relaxant activity. Samples eluted with CHCl3/MeOH (95:5) had moderate relaxant activity, while a second distinctive peak of activity eluted with a more polar solvent mixture (CHCl3/MeOH 50:50) provided strong dose dependent responses. Evidence was obtained that there are at least two components of raspberry leaf extract which exhibit relaxant activity in an in vitro gastrointestinal preparation. Copyright © 2002 John Wiley & Sons, Ltd.
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Relaxant activity of raspberry ( Rubus idaeus) leaf extract in guinea‐pig ileum in vitro
Phytotherapy research : PTR, 2002Co-Authors: J. Rojas-vera, Asmita V Patel, C. G. DackeAbstract:Tea made from the leaves of Rubus idaeus L. (raspberry) has been used for centuries as a folk medicine to treat wounds, diarrhoea, colic pain and as a uterine relaxant. Extracts of dried raspberry leaves prepared with different solvents, (n-hexane, ethyl acetate, chloroform and methanol) were tested in vitro for relaxant activity on transmurally stimulated guinea-pig ileum. The methanol (MeOH) extract exhibited the largest response and also indicated that the active compounds are of a relatively polar nature. Hence the bulk of the leaves were extracted with methanol and the dried extract fractionated on a silica gel column, eluting with chloroform, mixtures of chloroform and methanol and finally methanol. Each fraction was examined by thin layer chromatography and tested for relaxant activity in an in vitro transmurally stimulated guinea-pig ileum preparation. The fractions eluted with chloroform (CHCl3) lacked relaxant activity. Samples eluted with CHCl3/MeOH (95:5) had moderate relaxant activity, while a second distinctive peak of activity eluted with a more polar solvent mixture (CHCl3/MeOH 50:50) provided strong dose dependent responses. Evidence was obtained that there are at least two components of raspberry leaf extract which exhibit relaxant activity in an in vitro gastrointestinal preparation. Copyright © 2002 John Wiley & Sons, Ltd.
C. G. Dacke - One of the best experts on this subject based on the ideXlab platform.
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relaxant activity of raspberry Rubus idaeus leaf extract in guinea pig ileum in vitro
Phytotherapy Research, 2002Co-Authors: Janne Rojasvera, Asmita V Patel, C. G. DackeAbstract:Tea made from the leaves of Rubus idaeus L. (raspberry) has been used for centuries as a folk medicine to treat wounds, diarrhoea, colic pain and as a uterine relaxant. Extracts of dried raspberry leaves prepared with different solvents, (n-hexane, ethyl acetate, chloroform and methanol) were tested in vitro for relaxant activity on transmurally stimulated guinea-pig ileum. The methanol (MeOH) extract exhibited the largest response and also indicated that the active compounds are of a relatively polar nature. Hence the bulk of the leaves were extracted with methanol and the dried extract fractionated on a silica gel column, eluting with chloroform, mixtures of chloroform and methanol and finally methanol. Each fraction was examined by thin layer chromatography and tested for relaxant activity in an in vitro transmurally stimulated guinea-pig ileum preparation. The fractions eluted with chloroform (CHCl3) lacked relaxant activity. Samples eluted with CHCl3/MeOH (95:5) had moderate relaxant activity, while a second distinctive peak of activity eluted with a more polar solvent mixture (CHCl3/MeOH 50:50) provided strong dose dependent responses. Evidence was obtained that there are at least two components of raspberry leaf extract which exhibit relaxant activity in an in vitro gastrointestinal preparation. Copyright © 2002 John Wiley & Sons, Ltd.
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Relaxant activity of raspberry ( Rubus idaeus) leaf extract in guinea‐pig ileum in vitro
Phytotherapy research : PTR, 2002Co-Authors: J. Rojas-vera, Asmita V Patel, C. G. DackeAbstract:Tea made from the leaves of Rubus idaeus L. (raspberry) has been used for centuries as a folk medicine to treat wounds, diarrhoea, colic pain and as a uterine relaxant. Extracts of dried raspberry leaves prepared with different solvents, (n-hexane, ethyl acetate, chloroform and methanol) were tested in vitro for relaxant activity on transmurally stimulated guinea-pig ileum. The methanol (MeOH) extract exhibited the largest response and also indicated that the active compounds are of a relatively polar nature. Hence the bulk of the leaves were extracted with methanol and the dried extract fractionated on a silica gel column, eluting with chloroform, mixtures of chloroform and methanol and finally methanol. Each fraction was examined by thin layer chromatography and tested for relaxant activity in an in vitro transmurally stimulated guinea-pig ileum preparation. The fractions eluted with chloroform (CHCl3) lacked relaxant activity. Samples eluted with CHCl3/MeOH (95:5) had moderate relaxant activity, while a second distinctive peak of activity eluted with a more polar solvent mixture (CHCl3/MeOH 50:50) provided strong dose dependent responses. Evidence was obtained that there are at least two components of raspberry leaf extract which exhibit relaxant activity in an in vitro gastrointestinal preparation. Copyright © 2002 John Wiley & Sons, Ltd.
J. Rojas-vera - One of the best experts on this subject based on the ideXlab platform.
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A Straight-Chain Alcohol Glycoside, with Smooth Muscle Relaxant Activity, from Rubus idaeus (Raspberry) Leaves:
Natural Product Communications, 2007Co-Authors: Asmita V Patel, Christopher G Dacke, Gerald Blunden, J. Rojas-veraAbstract:A methanolic extract of Rubus idaeus leaves, when fractionated by column chromatography, yielded two distinct bands containing compounds that demonstrated in vitro, dose dependent, smooth muscle re...
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Triterpenoids, Including One with Smooth Muscle Relaxant Activity, from Rubus idaeus (Raspberry) Leaves:
Natural Product Communications, 2006Co-Authors: J. Rojas-vera, Christopher G Dacke, Asmita V Patel, Gerald BlundenAbstract:A methanol extract of Rubus idaeus leaves was fractionated by column chromatography and the eluted samples were tested for their ability to relax transmurally stimulated Guinea pig ileum preparatio...
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Relaxant activity of raspberry ( Rubus idaeus) leaf extract in guinea‐pig ileum in vitro
Phytotherapy research : PTR, 2002Co-Authors: J. Rojas-vera, Asmita V Patel, C. G. DackeAbstract:Tea made from the leaves of Rubus idaeus L. (raspberry) has been used for centuries as a folk medicine to treat wounds, diarrhoea, colic pain and as a uterine relaxant. Extracts of dried raspberry leaves prepared with different solvents, (n-hexane, ethyl acetate, chloroform and methanol) were tested in vitro for relaxant activity on transmurally stimulated guinea-pig ileum. The methanol (MeOH) extract exhibited the largest response and also indicated that the active compounds are of a relatively polar nature. Hence the bulk of the leaves were extracted with methanol and the dried extract fractionated on a silica gel column, eluting with chloroform, mixtures of chloroform and methanol and finally methanol. Each fraction was examined by thin layer chromatography and tested for relaxant activity in an in vitro transmurally stimulated guinea-pig ileum preparation. The fractions eluted with chloroform (CHCl3) lacked relaxant activity. Samples eluted with CHCl3/MeOH (95:5) had moderate relaxant activity, while a second distinctive peak of activity eluted with a more polar solvent mixture (CHCl3/MeOH 50:50) provided strong dose dependent responses. Evidence was obtained that there are at least two components of raspberry leaf extract which exhibit relaxant activity in an in vitro gastrointestinal preparation. Copyright © 2002 John Wiley & Sons, Ltd.
Janne Rojasvera - One of the best experts on this subject based on the ideXlab platform.
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relaxant activity of raspberry Rubus idaeus leaf extract in guinea pig ileum in vitro
Phytotherapy Research, 2002Co-Authors: Janne Rojasvera, Asmita V Patel, C. G. DackeAbstract:Tea made from the leaves of Rubus idaeus L. (raspberry) has been used for centuries as a folk medicine to treat wounds, diarrhoea, colic pain and as a uterine relaxant. Extracts of dried raspberry leaves prepared with different solvents, (n-hexane, ethyl acetate, chloroform and methanol) were tested in vitro for relaxant activity on transmurally stimulated guinea-pig ileum. The methanol (MeOH) extract exhibited the largest response and also indicated that the active compounds are of a relatively polar nature. Hence the bulk of the leaves were extracted with methanol and the dried extract fractionated on a silica gel column, eluting with chloroform, mixtures of chloroform and methanol and finally methanol. Each fraction was examined by thin layer chromatography and tested for relaxant activity in an in vitro transmurally stimulated guinea-pig ileum preparation. The fractions eluted with chloroform (CHCl3) lacked relaxant activity. Samples eluted with CHCl3/MeOH (95:5) had moderate relaxant activity, while a second distinctive peak of activity eluted with a more polar solvent mixture (CHCl3/MeOH 50:50) provided strong dose dependent responses. Evidence was obtained that there are at least two components of raspberry leaf extract which exhibit relaxant activity in an in vitro gastrointestinal preparation. Copyright © 2002 John Wiley & Sons, Ltd.
Shao-jiang Song - One of the best experts on this subject based on the ideXlab platform.
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Chiral-phase resolution of sesquilignans from raspberries (Rubus idaeus L.) and their neuroprotective effects.
Fitoterapia, 2020Co-Authors: Le-zhou, Zi-lin Hou, Jie-wang, Guo-dong Yao, Bin Lin, Xiao-xiao Huang, Shao-jiang SongAbstract:Abstract Two pairs of diastereoisomers (1/2 and 3/4) were isolated from the fruits of Rubus idaeus L. (Rosaceae). Their structures were elucidated on the basis of extensive spectroscopic analyses. Then chiral-phase HPLC resolution gave 1a/1b-4a/4b. Their absolute configurations were determined by comparison of the experimental ECD with the calculated data. Moreover, all isolated compounds were investigated for the neuroprotective effects against H2O2-induced neurotoxicity in human neuroblastoma SH-SY5Y cells, and 2a (66.04%) exhibited moderate neuroprotective effects, better than trolox (60.54%) at the concentration of 25 μM.
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Isolation of enantiomeric furolactones and furofurans from Rubus idaeus L. with neuroprotective activities.
Phytochemistry, 2019Co-Authors: Le Zhou, Guo-dong Yao, Bin Lin, Xiao-xiao Huang, Feng-ying Han, Ying-ying Zhang, Xiao-bo Wang, Shao-jiang SongAbstract:Abstract A phytochemical study on the fruits of Rubus idaeus L. (Rosaceae) yielded eight pairs of enantiomeric lignans, including one undescribed furolactone named (−)-idaeusinol A and six undescribed furofuran derivatives named (+/−)-idaeusinol B–D. The structures of these isolated compounds were elucidated by spectroscopic analyses and a combination of computational techniques including gauge-independent atomic orbital (GIAO) calculation of 1D NMR data and TD-DFT calculation of electronic circular dichroism (ECD) spectra. Bioactivity screenings suggested that (+)-idaeusinol D exhibited the most significant protective effect against H2O2-induced neurotoxicity at the concentration of 25 μM. In contrast, (−)-idaeusinol D, as the enantiomer of (+)-idaeusinol D, showed no effect against H2O2-induced neurotoxicity at both 25 and 50 μM concentration.
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neolignans from red raspberry Rubus idaeus l exhibit enantioselective neuroprotective effects against h2o2 induced oxidative injury in sh sy5y cells
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Le Zhou, Guo-dong Yao, Bin Lin, Xiao-xiao Huang, Xiao-bo Wang, Xiaoyu Song, Shao-jiang SongAbstract:Red raspberry has been well-known for its nutritional purpose. Although this fruit has been reported for its potent antioxidant activity and health-promoting properties, systematic studies responsible for the bioactive constituents were still insufficient. In the current study, three pairs of dihydrobenzofuran-type enantiomeric neolignans (1a/1b–3a/3b), including two new compounds (1b and 2a), were isolated from the fruit of Rubus idaeus. The structures of these enantiomers were determined through spectroscopic methods and quantum mechanical calculations. Biologically, enantiomers 2a and 2b exhibited significant enantioselective protective effects against H2O2-induced neurotoxicity at 50 μM (2a, 86.72 ± 1.17%; 2b, 69.70 ± 1.59%). The underlying mechanism study demonstrated that enantiomer 2a is able to attenuate H2O2-induced apoptosis, reactive oxygen species (ROS) generation, and mitochondrial dysfunction in SH-SY5Y cells. Overall, these findings provide a valuable foundation for the understanding of neu...
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Bioactive lignans and flavones with in vitro antioxidant and neuroprotective properties from Rubus idaeus rhizome
Journal of Functional Foods, 2017Co-Authors: Qian Cong, Wei Wang, Ying Peng, Shao-jiang SongAbstract:Rubus idaeus L. is caneberries from the genus Rubus that have been cultivated as food and herbal medicine, and is known to demonstrate strong antioxidant capability. In the present study, five new lignans, along with 13 known compounds, were isolated from ethanol extracts of R. idaeus rhizomes. An HPLC-MS/MS method was established and successfully applied to quantification of 14 compounds from leaves (LR), fruits (FR) and rhizomes (RR) of R. idaeus. All the compounds showed in vitro antioxidant activities, and three lignans revealed in vitro neuroprotective protective effects against H2O2-induced neurodegeneration in SH-SY5Y cells, the crude RR, LR and FR were found to have both in vitro antioxidant activities and neuroprotective effects.
