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Li Yongfei - One of the best experts on this subject based on the ideXlab platform.
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Comparison of the specificity of Chinese Sacbrood Virus (CSBV) TaqMan PCR assay for CSBV detection.
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Chinese Sacbrood Virus strains gave a positive reaction using the TaqMan PCR assay method, no cross-reactivity with other clinically related Viruses, including deformed wing Virus (DWV), black queen cell Virus (BQCV), acute been paralysis Virus (ABPV), and chronic bee paralysis Virus (CBPV), was detected.
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Detection of 37 Chinese Sacbrood Virus (CSBV) clinical samples: of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative PCR, reverse transcriptase polymerase chain reaction (RT-PCR) and electron microscopy methods.
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Detection of 37 Chinese Sacbrood Virus (CSBV) clinical samples: of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative PCR, reverse transcriptase polymerase chain reaction (RT-PCR) and electron microscopy methods.
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Reproducibility of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative polymerase chain reaction (PCR).
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:The reproducibility of the Chinese Sacbrood Virus (CSBV) TaqMan PCR assay was demonstrated by evaluating the variability of the CT values obtained after amplification of 10-fold serial dilutions of the plasmid DNA standard ranging from 101 to 106 copies per reaction in triplicate during the same experiment. The coefficient of variation (CV) of the mean CT values obtained for the DNA standard curve ranged from 0.27 to 1.4%.
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TaqMan MGB Probe Fluorescence Real-Time Quantitative PCR for Rapid Detection of Chinese Sacbrood Virus
2012Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Sacbrood Virus (SBV) is a picorna-like Virus that affects honey bees (Apis mellifera) and results in the death of the larvae. Several procedures are available to detect Chinese SBV (CSBV) in clinical samples, but not to estimate the level of CSBV infection. The aim of this study was develop an assay for rapid detection and quantification of this Virus. Primers and probes were designed that were specific for CSBV structural protein genes. A TaqMan minor groove binder (MGB) probe-based, fluorescence real-time quantitative PCR was established. The specificity, sensitivity and stability of the assay were assessed; specificity was high and there were no cross-reactivity with healthy larvae or other bee Viruses. The assay was applied to detect CSBV in 37 clinical samples and its efficiency was compared with clinical diagnosis, electron microscopy observation, and conventional RT-PCR. The TaqMan MGB-based probe fluorescence real-time quantitative PCR for CSBV was more sensitive than other methods tested. This assay was a reliable, fast, and sensitive method that was used successfully to detect CSBV in clinical samples. The technology can provide a useful tool for rapid detection of CSBV. This study has established a useful protocol for CSBV testing, epidemiological investigation, and development of animal models
Ma Mingxiao - One of the best experts on this subject based on the ideXlab platform.
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Comparison of the specificity of Chinese Sacbrood Virus (CSBV) TaqMan PCR assay for CSBV detection.
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Chinese Sacbrood Virus strains gave a positive reaction using the TaqMan PCR assay method, no cross-reactivity with other clinically related Viruses, including deformed wing Virus (DWV), black queen cell Virus (BQCV), acute been paralysis Virus (ABPV), and chronic bee paralysis Virus (CBPV), was detected.
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Detection of 37 Chinese Sacbrood Virus (CSBV) clinical samples: of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative PCR, reverse transcriptase polymerase chain reaction (RT-PCR) and electron microscopy methods.
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Detection of 37 Chinese Sacbrood Virus (CSBV) clinical samples: of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative PCR, reverse transcriptase polymerase chain reaction (RT-PCR) and electron microscopy methods.
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Reproducibility of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative polymerase chain reaction (PCR).
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:The reproducibility of the Chinese Sacbrood Virus (CSBV) TaqMan PCR assay was demonstrated by evaluating the variability of the CT values obtained after amplification of 10-fold serial dilutions of the plasmid DNA standard ranging from 101 to 106 copies per reaction in triplicate during the same experiment. The coefficient of variation (CV) of the mean CT values obtained for the DNA standard curve ranged from 0.27 to 1.4%.
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TaqMan MGB Probe Fluorescence Real-Time Quantitative PCR for Rapid Detection of Chinese Sacbrood Virus
2012Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Sacbrood Virus (SBV) is a picorna-like Virus that affects honey bees (Apis mellifera) and results in the death of the larvae. Several procedures are available to detect Chinese SBV (CSBV) in clinical samples, but not to estimate the level of CSBV infection. The aim of this study was develop an assay for rapid detection and quantification of this Virus. Primers and probes were designed that were specific for CSBV structural protein genes. A TaqMan minor groove binder (MGB) probe-based, fluorescence real-time quantitative PCR was established. The specificity, sensitivity and stability of the assay were assessed; specificity was high and there were no cross-reactivity with healthy larvae or other bee Viruses. The assay was applied to detect CSBV in 37 clinical samples and its efficiency was compared with clinical diagnosis, electron microscopy observation, and conventional RT-PCR. The TaqMan MGB-based probe fluorescence real-time quantitative PCR for CSBV was more sensitive than other methods tested. This assay was a reliable, fast, and sensitive method that was used successfully to detect CSBV in clinical samples. The technology can provide a useful tool for rapid detection of CSBV. This study has established a useful protocol for CSBV testing, epidemiological investigation, and development of animal models
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Molecular and Biological Characterization of Chinese Sacbrood Virus LN Isolate
Hindawi Limited, 2011Co-Authors: Ma Mingxiao, Li Ming, Cheng Jian, Yang Song, Wang Shude, Li PengfeiAbstract:Chinese Sacbrood Virus (CSBV) was purified from diseased insects, and its genome was cloned and sequenced. The genomic RNA of CSBV is 8863 nucleotides in length and contains a single large open reading frame encoding a 319.614 kDa polyprotein. The coding sequence is flanked by a 178-nucleotide 5′ nontranslated leader sequence and a 142-nucleotide 3′ nontranslated region, followed a poly(A) tail. Four major structural proteins, VP1,VP2, VP3 and VP4, were predicted in the N-teminal of the polyprotein. The C-terminal part of the polyprotein contains sequence motifs which is a typical and well-characterized picornaVirus nonstructural proteins: an RNA helicase, a chymotrypsin-like 3C protease, and an RNA-dependent RNA polymerase. Genetic analysis shows that the CSBV-LN had a 13-amino-acid deletion at amino acid positions 710–719 and 727–729 in comparison with CSBV-GZ and SBV-UK, and the SBV-UK had a 7-amino-acid deletion at amino acid positions 2124–2132 in comparison with CSBV-GZ and CSBV-LN, and the CSBV-GZ and CSBV-LN had a 6-amino-acid deletion at amino acid positions 2143–2150 in comparison with SBV-UK. Phylogenetic analysis using RdRp of selected picorna-like Viruses shows that CSBV/SBV and Deformed Wing Virus (DWV) tend to group together, which possesses an RNA of similar size and gene order
Galosi, Cecilia Mónica - One of the best experts on this subject based on the ideXlab platform.
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Primeira detecção molecular de co-infecção de vírus de abelhas em Bombus atratus assintomática na América do Sul
2019Co-Authors: Reynaldi, Francisco José, Sguazza, Guillermo Hernán, Albicoro, Francisco Javier, Pecoraro, Marcelo Ricardo Ítalo, Galosi, Cecilia MónicaAbstract:Pollination is critical for food production and has the particularity of linking natural ecosystems with agricultural production systems. Recently, losses of bumblebee species have been reported worldwide. In this study, samples from a commercial exploitation of bumblebees of Argentina with a recent history of deaths were studied using a multiplex PCR for the detection of the honey bee Viruses most frequently detected in South America. All samples analysed were positive for co-infections with Deformed wing Virus, Black queen cell Virus and Sacbrood Virus. This is the first report of infection of Bombus atratus with honey bee Viruses. A better understanding of viral infections in bumblebees and of the epidemiology of Viruses could be of great importance as bumblebees can serve as possible viral reservoirs, resulting in pathogen spillover towards honey bees and native bumblebees.A polinização é essencial para a produção de alimentos e tem como particularidade a conexão entre os ecossistemas naturais com sistemas de produção agrícola. Recentemente, as perdas de espécies de bumblebee em todo o mundo têm sido relatadas. Neste trabalho, amostras de uma exploração comercial de bumblebee da Argentina, com recente história de mortes foram estudadas utilizando uma Multiplex PCR para a detecção de vírus de abelha mais frequentemente detectados na América do Sul. Todas as amostras analisadas foram positivas para as co-infecções com Deformed wing Virus, Black queen cell Viruses e Sacbrood Virus. Este trabalho descreve o primeiro relato de infecção de Bombus atratus com vírus de abelhas. Uma melhor compreensão das infecções virais em bumblebee e da epidemiologia dos vírus poderia ser de grande importância, uma vez que tais abelhas podem servir como reservatório viral, com possível repercussão tanto na produtividade de abelhas melíferas como afetando-as diretamente.Facultad de Ciencias Veterinaria
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Primeira detecção molecular de co-infecção de vírus de abelhas em Bombus atratus assintomática na América do Sul
Instituto Internacional de Ecologia, 2016Co-Authors: Reynaldi, Francisco José, Sguazza, Guillermo Hernán, Albicoro, Francisco Javier, Pecoraro, Marcelo Ricardo, Galosi, Cecilia MónicaAbstract:Pollination is critical for food production and has the particularity of linking natural ecosystems with agricultural production systems. Recently, losses of bumblebee species have been reported worldwide. In this study, samples from a commercial exploitation of bumblebees of Argentina with a recent history of deaths were studied using a multiplex PCR for the detection of the honey bee Viruses most frequently detected in South America. All samples analyzed were positive for co-infections with Deformed Wing Virus, Black Queen Cell Virus and Sacbrood Virus. This is the first report of infection of Bombus atratus with honey bee Viruses. A better understanding of viral infections in bumblebees and of the epidemiology of Viruses could be of great importance as bumblebees can serve as possible viral reservoirs, resulting in pathogen spillover towards honey bees and native bumblebees.A polinização é essencial para a produção de alimentos e tem como particularidade a conexão entre os ecossistemas naturais com sistemas de produção agrícola. Recentemente, as perdas de espécies de bumblebee em todo o mundo têm sido relatadas. Neste trabalho, amostras de uma exploração comercial de bumblebee da Argentina, com recente história de mortes foram estudadas utilizando uma Multiplex PCR para a detecção de vírus de abelha mais frequentemente detectados na América do Sul. Todas as amostras analisadas foram positivas para as co-infecções com Deformed wing Virus, Black queen cell Viruses e Sacbrood Virus. Este trabalho descreve o primeiro relato de infecção de Bombus atratus com vírus de abelhas. Uma melhor compreensão das infecções virais em bumblebee e da epidemiologia dos vírus poderia ser de grande importância, uma vez que tais abelhas podem servir como reservatório viral, com possível repercussão tanto na produtividade de abelhas melíferas como afetando-as diretamente.Fil: Reynaldi, Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Sguazza, Guillermo Hernán. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Albicoro, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Pecoraro, Marcelo Ricardo. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Galosi, Cecilia Monica. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentin
Liu Jinhua - One of the best experts on this subject based on the ideXlab platform.
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Comparison of the specificity of Chinese Sacbrood Virus (CSBV) TaqMan PCR assay for CSBV detection.
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Chinese Sacbrood Virus strains gave a positive reaction using the TaqMan PCR assay method, no cross-reactivity with other clinically related Viruses, including deformed wing Virus (DWV), black queen cell Virus (BQCV), acute been paralysis Virus (ABPV), and chronic bee paralysis Virus (CBPV), was detected.
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Detection of 37 Chinese Sacbrood Virus (CSBV) clinical samples: of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative PCR, reverse transcriptase polymerase chain reaction (RT-PCR) and electron microscopy methods.
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Detection of 37 Chinese Sacbrood Virus (CSBV) clinical samples: of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative PCR, reverse transcriptase polymerase chain reaction (RT-PCR) and electron microscopy methods.
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Reproducibility of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative polymerase chain reaction (PCR).
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:The reproducibility of the Chinese Sacbrood Virus (CSBV) TaqMan PCR assay was demonstrated by evaluating the variability of the CT values obtained after amplification of 10-fold serial dilutions of the plasmid DNA standard ranging from 101 to 106 copies per reaction in triplicate during the same experiment. The coefficient of variation (CV) of the mean CT values obtained for the DNA standard curve ranged from 0.27 to 1.4%.
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TaqMan MGB Probe Fluorescence Real-Time Quantitative PCR for Rapid Detection of Chinese Sacbrood Virus
2012Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Sacbrood Virus (SBV) is a picorna-like Virus that affects honey bees (Apis mellifera) and results in the death of the larvae. Several procedures are available to detect Chinese SBV (CSBV) in clinical samples, but not to estimate the level of CSBV infection. The aim of this study was develop an assay for rapid detection and quantification of this Virus. Primers and probes were designed that were specific for CSBV structural protein genes. A TaqMan minor groove binder (MGB) probe-based, fluorescence real-time quantitative PCR was established. The specificity, sensitivity and stability of the assay were assessed; specificity was high and there were no cross-reactivity with healthy larvae or other bee Viruses. The assay was applied to detect CSBV in 37 clinical samples and its efficiency was compared with clinical diagnosis, electron microscopy observation, and conventional RT-PCR. The TaqMan MGB-based probe fluorescence real-time quantitative PCR for CSBV was more sensitive than other methods tested. This assay was a reliable, fast, and sensitive method that was used successfully to detect CSBV in clinical samples. The technology can provide a useful tool for rapid detection of CSBV. This study has established a useful protocol for CSBV testing, epidemiological investigation, and development of animal models
Song Yingjin - One of the best experts on this subject based on the ideXlab platform.
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Comparison of the specificity of Chinese Sacbrood Virus (CSBV) TaqMan PCR assay for CSBV detection.
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Chinese Sacbrood Virus strains gave a positive reaction using the TaqMan PCR assay method, no cross-reactivity with other clinically related Viruses, including deformed wing Virus (DWV), black queen cell Virus (BQCV), acute been paralysis Virus (ABPV), and chronic bee paralysis Virus (CBPV), was detected.
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Detection of 37 Chinese Sacbrood Virus (CSBV) clinical samples: of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative PCR, reverse transcriptase polymerase chain reaction (RT-PCR) and electron microscopy methods.
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Detection of 37 Chinese Sacbrood Virus (CSBV) clinical samples: of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative PCR, reverse transcriptase polymerase chain reaction (RT-PCR) and electron microscopy methods.
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Reproducibility of TaqMan minor groove binder (MGB) probe fluorescence real-time quantitative polymerase chain reaction (PCR).
2013Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:The reproducibility of the Chinese Sacbrood Virus (CSBV) TaqMan PCR assay was demonstrated by evaluating the variability of the CT values obtained after amplification of 10-fold serial dilutions of the plasmid DNA standard ranging from 101 to 106 copies per reaction in triplicate during the same experiment. The coefficient of variation (CV) of the mean CT values obtained for the DNA standard curve ranged from 0.27 to 1.4%.
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TaqMan MGB Probe Fluorescence Real-Time Quantitative PCR for Rapid Detection of Chinese Sacbrood Virus
2012Co-Authors: Ma Mingxiao, Liu Jinhua, Song Yingjin, Li YongfeiAbstract:Sacbrood Virus (SBV) is a picorna-like Virus that affects honey bees (Apis mellifera) and results in the death of the larvae. Several procedures are available to detect Chinese SBV (CSBV) in clinical samples, but not to estimate the level of CSBV infection. The aim of this study was develop an assay for rapid detection and quantification of this Virus. Primers and probes were designed that were specific for CSBV structural protein genes. A TaqMan minor groove binder (MGB) probe-based, fluorescence real-time quantitative PCR was established. The specificity, sensitivity and stability of the assay were assessed; specificity was high and there were no cross-reactivity with healthy larvae or other bee Viruses. The assay was applied to detect CSBV in 37 clinical samples and its efficiency was compared with clinical diagnosis, electron microscopy observation, and conventional RT-PCR. The TaqMan MGB-based probe fluorescence real-time quantitative PCR for CSBV was more sensitive than other methods tested. This assay was a reliable, fast, and sensitive method that was used successfully to detect CSBV in clinical samples. The technology can provide a useful tool for rapid detection of CSBV. This study has established a useful protocol for CSBV testing, epidemiological investigation, and development of animal models
