The Experts below are selected from a list of 162 Experts worldwide ranked by ideXlab platform
Monica Hofte - One of the best experts on this subject based on the ideXlab platform.
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redox active pyocyanin secreted by pseudomonas aeruginosa 7nsk2 triggers systemic resistance to magnaporthe grisea but enhances rhizoctonia solani susceptibility in rice
Molecular Plant-microbe Interactions, 2006Co-Authors: David De Vleesschauwer, Pierre Cornelis, Monica HofteAbstract:Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the Salicylic Acid-Derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyanin production led to a significant decrease in induced systemic resistance (ISR) to M. grisea, and in trans complementation for pyocyanin production restored the ability to elicit ISR. Intriguingly, pyocyanin-deficient mutants, unlike the wild type, triggered ISR against R. solani. Hence, bacterial pyocyanin plays a differential role in 7NSK2-mediated ISR in rice. Application of purified pyocyanin to hydroponically grown rice seedlings increased H2O2 levels locally on the root surface as well as a biphasic H2O2 generation pattern in distal leaves. Co-application of pyocyanin and the antioxidant sodium ascorbate alle...
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redox active pyocyanin secreted by pseudomonas aeruginosa 7nsk2 triggers systemic resistance to magnaporthe grisea but enhances rhizoctonia solani susceptibility in rice
Molecular Plant-microbe Interactions, 2006Co-Authors: David De Vleesschauwer, Pierre Cornelis, Monica HofteAbstract:Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the Salicylic Acid-Derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyanin production led to a significant decrease in induced systemic resistance (ISR) to M. grisea, and in trans complementation for pyocyanin production restored the ability to elicit ISR. Intriguingly, pyocyanin-deficient mutants, unlike the wild type, triggered ISR against R. solani. Hence, bacterial pyocyanin plays a differential role in 7NSK2-mediated ISR in rice. Application of purified pyocyanin to hydroponically grown rice seedlings increased H202 levels locally on the root surface as well as a biphasic H202 generation pattern in distal leaves. Co-application of pyocyanin and the antioxidant sodium ascorbate alleviated the opposite effects of pyocyanin on rice blast and sheath blight development, suggesting that the differential effectiveness of pyocyanin with respect to 7NSK2-triggered ISR is mediated by transiently elevated H202 levels in planta. The cumulative results suggest that reactive oxygen species act as a double-edged sword in the interaction of rice with the hemibiotroph M. grisea and the necrotroph R. solani.
David De Vleesschauwer - One of the best experts on this subject based on the ideXlab platform.
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redox active pyocyanin secreted by pseudomonas aeruginosa 7nsk2 triggers systemic resistance to magnaporthe grisea but enhances rhizoctonia solani susceptibility in rice
Molecular Plant-microbe Interactions, 2006Co-Authors: David De Vleesschauwer, Pierre Cornelis, Monica HofteAbstract:Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the Salicylic Acid-Derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyanin production led to a significant decrease in induced systemic resistance (ISR) to M. grisea, and in trans complementation for pyocyanin production restored the ability to elicit ISR. Intriguingly, pyocyanin-deficient mutants, unlike the wild type, triggered ISR against R. solani. Hence, bacterial pyocyanin plays a differential role in 7NSK2-mediated ISR in rice. Application of purified pyocyanin to hydroponically grown rice seedlings increased H2O2 levels locally on the root surface as well as a biphasic H2O2 generation pattern in distal leaves. Co-application of pyocyanin and the antioxidant sodium ascorbate alle...
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redox active pyocyanin secreted by pseudomonas aeruginosa 7nsk2 triggers systemic resistance to magnaporthe grisea but enhances rhizoctonia solani susceptibility in rice
Molecular Plant-microbe Interactions, 2006Co-Authors: David De Vleesschauwer, Pierre Cornelis, Monica HofteAbstract:Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the Salicylic Acid-Derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyanin production led to a significant decrease in induced systemic resistance (ISR) to M. grisea, and in trans complementation for pyocyanin production restored the ability to elicit ISR. Intriguingly, pyocyanin-deficient mutants, unlike the wild type, triggered ISR against R. solani. Hence, bacterial pyocyanin plays a differential role in 7NSK2-mediated ISR in rice. Application of purified pyocyanin to hydroponically grown rice seedlings increased H202 levels locally on the root surface as well as a biphasic H202 generation pattern in distal leaves. Co-application of pyocyanin and the antioxidant sodium ascorbate alleviated the opposite effects of pyocyanin on rice blast and sheath blight development, suggesting that the differential effectiveness of pyocyanin with respect to 7NSK2-triggered ISR is mediated by transiently elevated H202 levels in planta. The cumulative results suggest that reactive oxygen species act as a double-edged sword in the interaction of rice with the hemibiotroph M. grisea and the necrotroph R. solani.
Pierre Cornelis - One of the best experts on this subject based on the ideXlab platform.
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redox active pyocyanin secreted by pseudomonas aeruginosa 7nsk2 triggers systemic resistance to magnaporthe grisea but enhances rhizoctonia solani susceptibility in rice
Molecular Plant-microbe Interactions, 2006Co-Authors: David De Vleesschauwer, Pierre Cornelis, Monica HofteAbstract:Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the Salicylic Acid-Derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyanin production led to a significant decrease in induced systemic resistance (ISR) to M. grisea, and in trans complementation for pyocyanin production restored the ability to elicit ISR. Intriguingly, pyocyanin-deficient mutants, unlike the wild type, triggered ISR against R. solani. Hence, bacterial pyocyanin plays a differential role in 7NSK2-mediated ISR in rice. Application of purified pyocyanin to hydroponically grown rice seedlings increased H2O2 levels locally on the root surface as well as a biphasic H2O2 generation pattern in distal leaves. Co-application of pyocyanin and the antioxidant sodium ascorbate alle...
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redox active pyocyanin secreted by pseudomonas aeruginosa 7nsk2 triggers systemic resistance to magnaporthe grisea but enhances rhizoctonia solani susceptibility in rice
Molecular Plant-microbe Interactions, 2006Co-Authors: David De Vleesschauwer, Pierre Cornelis, Monica HofteAbstract:Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the Salicylic Acid-Derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyanin production led to a significant decrease in induced systemic resistance (ISR) to M. grisea, and in trans complementation for pyocyanin production restored the ability to elicit ISR. Intriguingly, pyocyanin-deficient mutants, unlike the wild type, triggered ISR against R. solani. Hence, bacterial pyocyanin plays a differential role in 7NSK2-mediated ISR in rice. Application of purified pyocyanin to hydroponically grown rice seedlings increased H202 levels locally on the root surface as well as a biphasic H202 generation pattern in distal leaves. Co-application of pyocyanin and the antioxidant sodium ascorbate alleviated the opposite effects of pyocyanin on rice blast and sheath blight development, suggesting that the differential effectiveness of pyocyanin with respect to 7NSK2-triggered ISR is mediated by transiently elevated H202 levels in planta. The cumulative results suggest that reactive oxygen species act as a double-edged sword in the interaction of rice with the hemibiotroph M. grisea and the necrotroph R. solani.
Mitsuo Takayama - One of the best experts on this subject based on the ideXlab platform.
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Influence of initial velocity of analytes on in-source decay products in MALDI mass spectrometry using Salicylic Acid Derivative matrices
International Journal of Mass Spectrometry, 2013Co-Authors: Daiki Asakawa, Motoshi Sakakura, Mitsuo TakayamaAbstract:Abstract The fragmentation and recombination process of peptide radicals during matrix-assisted laser desorption/ionization (MALDI) in-source decay (ISD) with Salicylic Acid Derivative matrices has been described. MALDI-ISD is initiated by hydrogen transfer between peptide and matrix. The use of reducing and oxidizing matrix leads to the formation of c ′/ z and a / x fragment pairs, respectively. The fragmentation of analyte radicals occurs via a uni-molecular dissociation processes and independently of the collision in MALDI plume. In contrast, hydrogen transfer between analyte radicals and matrix molecules occurs by collision in MALDI plume as a bi-molecular process. The rate of collision in MALDI plume could be estimated by initial velocity of analyte ions and it affect on the yields of ISD fragments. Considering the initial velocity of analyte ions allows better understanding the MALDI-ISD process.
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text c _ alpha text c bond cleavage of the peptide backbone in maldi in source decay using Salicylic Acid Derivative matrices
Journal of the American Society for Mass Spectrometry, 2011Co-Authors: Daiki Asakawa, Mitsuo TakayamaAbstract:The use of 5-formylSalicylic Acid (5-FSA) and 5-nitroSalicylic Acid (5-NSA) as novel matrices for in-source decay (ISD) of peptides in matrix-assisted laser desorption/ionization (MALDI) is described. The use of 5-FSA and 5-NSA generated a- and x-series ions accompanied by oxidized peptides [M – 2 H + H]+. The preferential formation of a- and x-series ions was found to be dependent on the hydrogen-accepting ability of matrix. The hydrogen-accepting ability estimated from the ratio of signal intensity of oxidized product [M – 2 H + H]+ to that of non-oxidized protonated molecule [M + H]+ of peptide was of the order 5-NSA > 5-FSA > 5-aminoSalicylic Acid (5-ASA) ≒ 2,5-dihydroxyl benzoic Acid (2,5-DHB) ≒ 0. The results suggest that the hydrogen transfer reaction from peptide to 5-FSA and 5-NSA occurs during the MALDI-ISD processes. The hydrogen abstraction from peptides results in the formation of oxidized peptides containing a radical site on the amide nitrogen with subsequent radical-induced cleavage at the \( {{\text{C}}_{\alpha }} - {\text{C}} \) bond, leading to the formation of a- and x-series ions. The most significant feature of MALDI-ISD with 5-FSA and 5-NSA is the specific cleavage of the \( {{\text{C}}_{\alpha }} - {\text{C}} \) bond of the peptide backbone without degradation of side-chain and post-translational modifications (PTM). The matrix provides a useful complementary method to conventional MALDI-ISD for amino Acid sequencing and site localization of PTMs in peptides.
Peter L Rinaldi - One of the best experts on this subject based on the ideXlab platform.
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nonanthocyanin secondary metabolites of black raspberry rubus occidentalis l fruits identification by hplc dad nmr hplc esi ms and esi ms ms analyses
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Liladhar Paudel, Faith J Wyzgoski, Joseph C Scheerens, Ann M Chanon, Neil R Reese, Danijela Smiljanic, Chrys Wesdemiotis, Joshua J Blakeslee, Ken M Riedl, Peter L RinaldiAbstract:Nonanthocyanin secondary metabolites potentially contributing to the antiproliferative bioactivity of black raspberry (Rubus occidentalis L.) fruits were extracted in ethyl acetate and isolated by semipreparative and analytical HPLC and analyzed by NMR, HPLC-ESI-MS, and ESI-MS/MS techniques. Here we present complete and partial structures of a variety of the chemical entities such as quercetin 3-glucoside, quercetin 3-rutinoside, myricetin glucoside, dihydrokaempferol glucoside, benzoic Acid β-d-glucopyranosyl ester, 3,4-dihydroxybenzoic Acid, epicatechin, caffeic Acid, p-coumaric Acid, p-coumaryl glucoside, p-coumaryl sugar ester, ellagic Acid, methyl ellagic Acid acetylpentose, methyl ellagic Acid valerylpentose, trans-piceid, phloretin glucoside (phloridzin), dihydrosinapic Acid, Salicylic Acid β-d-glucopyranosyl ester, a Salicylic Acid Derivative without attached sugar, p-alkylphenyl glucoside, and a citric Acid Derivative. To our knowledge, 15 of these compounds were not previously reported in black ...
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Nonanthocyanin Secondary Metabolites of Black Raspberry (Rubus occidentalis L.) Fruits: Identification by HPLC-DAD, NMR, HPLC-ESI-MS, and ESI-MS/MS Analyses
2013Co-Authors: Liladhar Paudel, Faith J Wyzgoski, Joseph C Scheerens, Ann M Chanon, Neil R Reese, Danijela Smiljanic, Chrys Wesdemiotis, Joshua J Blakeslee, Ken M Riedl, Peter L RinaldiAbstract:Nonanthocyanin secondary metabolites potentially contributing to the antiproliferative bioactivity of black raspberry (Rubus occidentalis L.) fruits were extracted in ethyl acetate and isolated by semipreparative and analytical HPLC and analyzed by NMR, HPLC-ESI-MS, and ESI-MS/MS techniques. Here we present complete and partial structures of a variety of the chemical entities such as quercetin 3-glucoside, quercetin 3-rutinoside, myricetin glucoside, dihydrokaempferol glucoside, benzoic Acid β-d-glucopyranosyl ester, 3,4-dihydroxybenzoic Acid, epicatechin, caffeic Acid, p-coumaric Acid, p-coumaryl glucoside, p-coumaryl sugar ester, ellagic Acid, methyl ellagic Acid acetylpentose, methyl ellagic Acid valerylpentose, trans-piceid, phloretin glucoside (phloridzin), dihydrosinapic Acid, Salicylic Acid β-d-glucopyranosyl ester, a Salicylic Acid Derivative without attached sugar, p-alkylphenyl glucoside, and a citric Acid Derivative. To our knowledge, 15 of these compounds were not previously reported in black raspberry fruits
