The Experts below are selected from a list of 10677 Experts worldwide ranked by ideXlab platform
Hiroki Saeki - One of the best experts on this subject based on the ideXlab platform.
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major allergen and its ige cross reactivity among Salmonid Fish roe allergy
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Yutaka Shimizu, Atsushi Nakamura, Hideki Kishimura, Akihiko Hara, Kazuhiko Watanabe, Hiroki SaekiAbstract:Yolk protein extracts were prepared from four kinds of Salmonid Fish roes, and the proteins that reacted with IgE were screened by immunoblotting using sera from 20 patients allergic to chum salmon...
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major allergen and its ige cross reactivity among Salmonid Fish roe allergy
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Yutaka Shimizu, Atsushi Nakamura, Hideki Kishimura, Akihiko Hara, Kazuhiko Watanabe, Hiroki SaekiAbstract:Yolk protein extracts were prepared from four kinds of Salmonid Fish roes, and the proteins that reacted with IgE were screened by immunoblotting using sera from 20 patients allergic to chum salmon roe. IgE cross-reactivities among the Salmonid yolk proteins were also investigated by competitive ELISA. The results were as follows: (1) The major protein components in Salmonid roes were lipovitellin and beta'-component, which are subfragments of vitellogenin. (2) Most sera from the patients showed IgE reactivity to beta'-component in all yolk protein extracts, and some of them also reacted to lipovitellin heavy chain or its light chain. (3) Salmonid beta'-component showed high similarity (>90%) in the N-terminal amino acid sequence. (4) All of the Salmonid yolk protein extracts inhibited the IgE reaction between patient sera and the chum salmon beta'-component. These findings indicate that the beta'-component in Salmonid roe is a common major allergen with strong IgE cross-reactivity.
Stephanie M Carlson - One of the best experts on this subject based on the ideXlab platform.
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indirect genetic control of migration in a Salmonid Fish
Biology Letters, 2020Co-Authors: Suzanne J Kelson, Stephanie M Carlson, Michael R MillerAbstract:Migration is a complex trait that often has genetic underpinnings. However, it is unclear if migratory behaviour itself is inherited (direct genetic control), or if the decision to migrate is instead the outcome of a set of physiological traits (indirect genetic control). For steelhead/rainbow trout (Oncorhynchus mykiss), migration is strongly linked to a large genomic region across their range. Here, we demonstrate a shared allelic basis between early life growth rate and migratory behaviour. Next, we demonstrate that early life growth differs among resident/migratory genotypes in wild juveniles several months prior to migration, with resident genotypes achieving a larger size in their first few months of life than migratory genotypes. We suggest that the genetic basis of migration is likely indirect and mediated by physiological traits such as growth rate. Evolutionary benefits of this indirect genetic mechanism likely include flexibility among individuals and persistence of life-history diversity within and among populations.
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partial migration alters population ecology and food chain length evidence from a Salmonid Fish
Ecosphere, 2020Co-Authors: Suzanne J Kelson, Mary E Power, Jacques C Finlay, Stephanie M CarlsonAbstract:Author(s): Kelson, Suzanne J; Power, Mary E; Finlay, Jacques C; Carlson, Stephanie M | Abstract: Many migratory species, from monarch butterflies to wildebeest, express partial migration, where only a subset of a population migrates. This intraspecific variation is likely to have large ecological consequences. We studied the ecological consequences of partial migration in a Salmonid Fish, Oncorhynchus mykiss , in coastal streams in California, USA. One ecotype, steelhead trout, migrates to the ocean, whereas the other, rainbow trout, completes its lifecycle in freshwater. Migration has a strong genetic basis in O. mykiss . In one stream, we found differences in the frequency of migration‐linked genotypes below and above a waterfall barrier (migratory allele frequency of 60% below vs. 31% above). Below the waterfall, in the migratory‐dominated region, the density of young Fish (l1 yr old) was approximately twice that in the resident‐dominated region above the waterfall (0.46 vs. 0.26 individuals/m2, respectively), presumably reflecting the higher fecundity of migratory females. Additionally, there were half as many older Fish (g1 yr old) in pools downstream of the waterfall (0.05 vs. 0.13 individuals/m2). In a second stream, between‐year variation in the dominance of migratory vs. resident Fish allowed us to explore differences in Fish density and size structure through time, and we found a consistent pattern. In brief, when migratory genotypes dominated, we found higher densities of young Fish and lower densities of older Fish, resulting in a simpler size structure, compared to when resident genotypes dominated. Moreover, large resident trout had a slightly higher trophic position than young Fish (3.92 vs. 3.42 in one creek and 3.77 vs. 3.17 in the other), quantified with stable isotope data. The difference in Fish size structure did not generate trophic cascades. Partial migration is widespread among migratory populations, as is phenotypic divergence between resident and migratory forms, suggesting the potential for widespread ecological effects arising from this common form of intraspecific variation.
Suzanne J Kelson - One of the best experts on this subject based on the ideXlab platform.
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indirect genetic control of migration in a Salmonid Fish
Biology Letters, 2020Co-Authors: Suzanne J Kelson, Stephanie M Carlson, Michael R MillerAbstract:Migration is a complex trait that often has genetic underpinnings. However, it is unclear if migratory behaviour itself is inherited (direct genetic control), or if the decision to migrate is instead the outcome of a set of physiological traits (indirect genetic control). For steelhead/rainbow trout (Oncorhynchus mykiss), migration is strongly linked to a large genomic region across their range. Here, we demonstrate a shared allelic basis between early life growth rate and migratory behaviour. Next, we demonstrate that early life growth differs among resident/migratory genotypes in wild juveniles several months prior to migration, with resident genotypes achieving a larger size in their first few months of life than migratory genotypes. We suggest that the genetic basis of migration is likely indirect and mediated by physiological traits such as growth rate. Evolutionary benefits of this indirect genetic mechanism likely include flexibility among individuals and persistence of life-history diversity within and among populations.
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partial migration alters population ecology and food chain length evidence from a Salmonid Fish
Ecosphere, 2020Co-Authors: Suzanne J Kelson, Mary E Power, Jacques C Finlay, Stephanie M CarlsonAbstract:Author(s): Kelson, Suzanne J; Power, Mary E; Finlay, Jacques C; Carlson, Stephanie M | Abstract: Many migratory species, from monarch butterflies to wildebeest, express partial migration, where only a subset of a population migrates. This intraspecific variation is likely to have large ecological consequences. We studied the ecological consequences of partial migration in a Salmonid Fish, Oncorhynchus mykiss , in coastal streams in California, USA. One ecotype, steelhead trout, migrates to the ocean, whereas the other, rainbow trout, completes its lifecycle in freshwater. Migration has a strong genetic basis in O. mykiss . In one stream, we found differences in the frequency of migration‐linked genotypes below and above a waterfall barrier (migratory allele frequency of 60% below vs. 31% above). Below the waterfall, in the migratory‐dominated region, the density of young Fish (l1 yr old) was approximately twice that in the resident‐dominated region above the waterfall (0.46 vs. 0.26 individuals/m2, respectively), presumably reflecting the higher fecundity of migratory females. Additionally, there were half as many older Fish (g1 yr old) in pools downstream of the waterfall (0.05 vs. 0.13 individuals/m2). In a second stream, between‐year variation in the dominance of migratory vs. resident Fish allowed us to explore differences in Fish density and size structure through time, and we found a consistent pattern. In brief, when migratory genotypes dominated, we found higher densities of young Fish and lower densities of older Fish, resulting in a simpler size structure, compared to when resident genotypes dominated. Moreover, large resident trout had a slightly higher trophic position than young Fish (3.92 vs. 3.42 in one creek and 3.77 vs. 3.17 in the other), quantified with stable isotope data. The difference in Fish size structure did not generate trophic cascades. Partial migration is widespread among migratory populations, as is phenotypic divergence between resident and migratory forms, suggesting the potential for widespread ecological effects arising from this common form of intraspecific variation.
Yutaka Shimizu - One of the best experts on this subject based on the ideXlab platform.
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major allergen and its ige cross reactivity among Salmonid Fish roe allergy
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Yutaka Shimizu, Atsushi Nakamura, Hideki Kishimura, Akihiko Hara, Kazuhiko Watanabe, Hiroki SaekiAbstract:Yolk protein extracts were prepared from four kinds of Salmonid Fish roes, and the proteins that reacted with IgE were screened by immunoblotting using sera from 20 patients allergic to chum salmon...
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major allergen and its ige cross reactivity among Salmonid Fish roe allergy
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Yutaka Shimizu, Atsushi Nakamura, Hideki Kishimura, Akihiko Hara, Kazuhiko Watanabe, Hiroki SaekiAbstract:Yolk protein extracts were prepared from four kinds of Salmonid Fish roes, and the proteins that reacted with IgE were screened by immunoblotting using sera from 20 patients allergic to chum salmon roe. IgE cross-reactivities among the Salmonid yolk proteins were also investigated by competitive ELISA. The results were as follows: (1) The major protein components in Salmonid roes were lipovitellin and beta'-component, which are subfragments of vitellogenin. (2) Most sera from the patients showed IgE reactivity to beta'-component in all yolk protein extracts, and some of them also reacted to lipovitellin heavy chain or its light chain. (3) Salmonid beta'-component showed high similarity (>90%) in the N-terminal amino acid sequence. (4) All of the Salmonid yolk protein extracts inhibited the IgE reaction between patient sera and the chum salmon beta'-component. These findings indicate that the beta'-component in Salmonid roe is a common major allergen with strong IgE cross-reactivity.
Makoto Okuno - One of the best experts on this subject based on the ideXlab platform.
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Regulation of Salmonid Fish sperm motility by osmotic shock-induced water influx across the plasma membrane
Comparative biochemistry and physiology. Part A Molecular & integrative physiology, 2014Co-Authors: L. Takei, Chinatsu Mukai, Makoto OkunoAbstract:Abstract The motility of Salmonid Fish sperm is initiated by a decrease in the extracellular K + concentration. However, our previous studies revealed that Salmonid Fish sperm motility could be initiated in the presence of an inhibitory concentration of K + by drastic osmotic shock induced by suspension in a hypertonic glycerol solution and subsequent dilution in a hypotonic solution (glycerol-treatment). In the present study, we examined if an osmotic shock-induced water influx is involved in the regulation of Salmonid Fish sperm motility. HgCl 2 , a common inhibitor of aquaporins (AQPs), decreased the duration of Salmonid Fish sperm motility. Dilution of sperm cells in a hypotonic solution increased the cellular volume, whereas HgCl 2 inhibited such an increase in cellular volume. Furthermore, the expression of AQP 1a and 10 in rainbow trout testes was confirmed. In contrast, HgCl 2 did not affect glycerol-treated sperm motility, indicating that AQPs are not involved in glycerol-treated sperm motility. We also explored the possibility of aquaporin-independent water influx in glycerol-treated sperm by assessing the sperm membrane permeability using propidium iodide. The plasma membrane of glycerol-treated sperm was considerably permeabilized. The cellular volume was decreased in a hypertonic glycerol solution and increased upon subsequent hypoosmotic shock, indicating an AQP-independent water flux across the plasma membrane upon glycerol-treatment. Taken together, these results showed that water influx across the plasma membrane via AQP is crucial for the maintenance of Salmonid Fish sperm motility under normal conditions, whereas water influx by osmotic shock-induced membrane permeation is critical for the initiation of glycerol-treated sperm motility.
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transient ca2 mobilization caused by osmotic shock initiates Salmonid Fish sperm motility
The Journal of Experimental Biology, 2012Co-Authors: Gen L Takei, Chinatsu Mukai, Makoto OkunoAbstract:Salmonid Fish sperm motility is known to be suppressed in millimolar concentrations of extracellular K(+), and dilution of K(+) upon spawning triggers cAMP-dependent signaling for motility initiation. In a previous study, however, we demonstrated that suspending sperm in a 10% glycerol solution and subsequent dilution into a low-osmotic solution induced motility independently of extracellular K(+) and cAMP. In the present study, we further investigated the glycerol-induced motility mechanism. We found that treatment with solutions consisting of organic or inorganic ions, as well as glycerol, induced sperm motility in an osmolarity-dependent manner. Elimination of intracellular Ca(2+) by BAPTA-AM significantly inhibited glycerol-treated sperm motility, whereas removal of extracellular Ca(2+) by EGTA did not. Monitoring intracellular Ca(2+), using fluo-4, revealed that intracellular Ca(2+) increased when sperm were suspended in hypertonic solutions, and a subsequent dilution into a hypotonic solution led to a decrease in intracellular Ca(2+) concomitant with motility initiation. In addition, upon dilution of sperm into a hypertonic glycerol solution prior to demembranation, the motility of demembranated sperm was reactivated in the absence of cAMP. The motility recovery suggests that completion of axonemal maturation occurred during exposure to a hypertonic environment. As a result, it is likely that glycerol treatment of sperm undergoing hypertonic shock causes mobilization of intracellular Ca(2+) from the intracellular Ca(2+) store and also causes maturation of axonemal proteins for motility initiation. The subsequent dilution into a hypotonic solution induces a decrease in intracellular Ca(2+) and flagellar movement. This novel mechanism of sperm motility initiation seems to act in a salvaging manner for the well-known K(+)-dependent pathway.
