The Experts below are selected from a list of 34737 Experts worldwide ranked by ideXlab platform
Randall J Anderson - One of the best experts on this subject based on the ideXlab platform.
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environmental Sampling to assess the bioburden of mycobacterium avium subspecies paratuberculosis in drylot pens on california dairies
PeerJ, 2019Co-Authors: Tapakorn Chamchoy, Deneice R Williams, John M Adaska, Randall J AndersonAbstract:Author(s): Chamchoy, Tapakorn; Williams, Deneice R; Adaska, John M; Anderson, Randall J; Aly, Sharif S | Abstract: Mycobacterium avium subspecies paratuberculosis (MAP) is a bacterium that can cause substantial economic losses in infected dairy herds due to reduced milk production and increased cow-replacement costs. In order to control MAP in dairies with drylot pens, a standardized environmental Sampling Protocol to quantify MAP in fecal slurry was developed based on an existing Protocol for freestall pens. Specifically, following a 24 h hold of the flush, a grab sample of approximately 10 ml of fecal slurry was collected every 1 m along the flush lane of the drylot pens, avoiding individual cow fecal pats. To determine the reliability and repatability of the new environmental Sampling Protocol for estimation of MAP bioburden at the pen level, two collectors simultaneously collected fecal slurry samples every day for 3 days from six drylot cow pens on two Central California dairies. During the study period no cow movement between pens was allowed with the exception of sick cows. The study herds had MAP seroprevalence of 5.8% and 3.2%, respectively, based on whole pen serum ELISA results. Variance components models for quantitative real-time PCR (qPCR) results showed samples collected from different pens on different dairies accounted for greater variablitiy in MAP concentration (65%), while samples collected by different collectors had the least variability (0.1%). In contrast, variability in MAP concentration in environmental samples collected on different days had 25% variability. The intraclass correlation coefficient showed high reliability (93%) of environmental Sampling simultaneously by different collectors. In contrast, the reliability of environmental Sampling at different days was 65%, which was similar to the reliability for Sampling by different collectors on different days. Investigators can expect high reliability when employing the new environmental Sampling Protocol along with qPCR testing of environmental samples from drylot pens.
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environmental Sampling to assess the bioburden of mycobacterium avium subspecies paratuberculosis in drylot pens on california dairies
PeerJ, 2019Co-Authors: Tapakorn Chamchoy, Deneice R Williams, John M Adaska, Randall J Anderson, Sharif S AlyAbstract:Mycobacterium avium subspecies paratuberculosis (MAP) is a bacterium that can cause substantial economic losses in infected dairy herds due to reduced milk production and increased cow-replacement costs. In order to control MAP in dairies with drylot pens, a standardized environmental Sampling Protocol to quantify MAP in fecal slurry was developed based on an existing Protocol for freestall pens. Specifically, following a 24 h hold of the flush, a grab sample of approximately 10 ml of fecal slurry was collected every 1 m along the flush lane of the drylot pens, avoiding individual cow fecal pats. To determine the reliability and repatability of the new environmental Sampling Protocol for estimation of MAP bioburden at the pen level, two collectors simultaneously collected fecal slurry samples every day for 3 days from six drylot cow pens on two Central California dairies. During the study period no cow movement between pens was allowed with the exception of sick cows. The study herds had MAP seroprevalence of 5.8% and 3.2%, respectively, based on whole pen serum ELISA results. Variance components models for quantitative real-time PCR (qPCR) results showed samples collected from different pens on different dairies accounted for greater variablitiy in MAP concentration (65%), while samples collected by different collectors had the least variability (0.1%). In contrast, variability in MAP concentration in environmental samples collected on different days had 25% variability. The intraclass correlation coefficient showed high reliability (93%) of environmental Sampling simultaneously by different collectors. In contrast, the reliability of environmental Sampling at different days was 65%, which was similar to the reliability for Sampling by different collectors on different days. Investigators can expect high reliability when employing the new environmental Sampling Protocol along with qPCR testing of environmental samples from drylot pens.
Tapakorn Chamchoy - One of the best experts on this subject based on the ideXlab platform.
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environmental Sampling to assess the bioburden of mycobacterium avium subspecies paratuberculosis in drylot pens on california dairies
PeerJ, 2019Co-Authors: Tapakorn Chamchoy, Deneice R Williams, John M Adaska, Randall J AndersonAbstract:Author(s): Chamchoy, Tapakorn; Williams, Deneice R; Adaska, John M; Anderson, Randall J; Aly, Sharif S | Abstract: Mycobacterium avium subspecies paratuberculosis (MAP) is a bacterium that can cause substantial economic losses in infected dairy herds due to reduced milk production and increased cow-replacement costs. In order to control MAP in dairies with drylot pens, a standardized environmental Sampling Protocol to quantify MAP in fecal slurry was developed based on an existing Protocol for freestall pens. Specifically, following a 24 h hold of the flush, a grab sample of approximately 10 ml of fecal slurry was collected every 1 m along the flush lane of the drylot pens, avoiding individual cow fecal pats. To determine the reliability and repatability of the new environmental Sampling Protocol for estimation of MAP bioburden at the pen level, two collectors simultaneously collected fecal slurry samples every day for 3 days from six drylot cow pens on two Central California dairies. During the study period no cow movement between pens was allowed with the exception of sick cows. The study herds had MAP seroprevalence of 5.8% and 3.2%, respectively, based on whole pen serum ELISA results. Variance components models for quantitative real-time PCR (qPCR) results showed samples collected from different pens on different dairies accounted for greater variablitiy in MAP concentration (65%), while samples collected by different collectors had the least variability (0.1%). In contrast, variability in MAP concentration in environmental samples collected on different days had 25% variability. The intraclass correlation coefficient showed high reliability (93%) of environmental Sampling simultaneously by different collectors. In contrast, the reliability of environmental Sampling at different days was 65%, which was similar to the reliability for Sampling by different collectors on different days. Investigators can expect high reliability when employing the new environmental Sampling Protocol along with qPCR testing of environmental samples from drylot pens.
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environmental Sampling to assess the bioburden of mycobacterium avium subspecies paratuberculosis in drylot pens on california dairies
PeerJ, 2019Co-Authors: Tapakorn Chamchoy, Deneice R Williams, John M Adaska, Randall J Anderson, Sharif S AlyAbstract:Mycobacterium avium subspecies paratuberculosis (MAP) is a bacterium that can cause substantial economic losses in infected dairy herds due to reduced milk production and increased cow-replacement costs. In order to control MAP in dairies with drylot pens, a standardized environmental Sampling Protocol to quantify MAP in fecal slurry was developed based on an existing Protocol for freestall pens. Specifically, following a 24 h hold of the flush, a grab sample of approximately 10 ml of fecal slurry was collected every 1 m along the flush lane of the drylot pens, avoiding individual cow fecal pats. To determine the reliability and repatability of the new environmental Sampling Protocol for estimation of MAP bioburden at the pen level, two collectors simultaneously collected fecal slurry samples every day for 3 days from six drylot cow pens on two Central California dairies. During the study period no cow movement between pens was allowed with the exception of sick cows. The study herds had MAP seroprevalence of 5.8% and 3.2%, respectively, based on whole pen serum ELISA results. Variance components models for quantitative real-time PCR (qPCR) results showed samples collected from different pens on different dairies accounted for greater variablitiy in MAP concentration (65%), while samples collected by different collectors had the least variability (0.1%). In contrast, variability in MAP concentration in environmental samples collected on different days had 25% variability. The intraclass correlation coefficient showed high reliability (93%) of environmental Sampling simultaneously by different collectors. In contrast, the reliability of environmental Sampling at different days was 65%, which was similar to the reliability for Sampling by different collectors on different days. Investigators can expect high reliability when employing the new environmental Sampling Protocol along with qPCR testing of environmental samples from drylot pens.
Fernando A Escobedo - One of the best experts on this subject based on the ideXlab platform.
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kinetics and reaction coordinate for the isomerization of alanine dipeptide by a forward flux Sampling Protocol
Journal of Chemical Physics, 2009Co-Authors: Camilo Velezvega, Ernesto E Borrero, Fernando A EscobedoAbstract:Forward flux Sampling (FFS) simulations were used to study the kinetics of alanine dipeptide both in vacuum and in explicit solvent. The recently proposed FFS least-squares estimation approach and an algorithm that optimizes the position of the interfaces were implemented to determine a reaction coordinate that adequately describes the transition dynamics. A new method is also introduced to try to ensure that the ensemble of "starting points" (for the trial trajectories) is properly sampled. The rate constant estimates for the C7(eq)-->C5 transition of alanine dipeptide in vacuum were used to demonstrate the consistency between Monte Carlo and molecular dynamics (MD) simulations. FFS-MD simulations were then performed for the study of the beta(2)/alpha(R)-->C5/C7(eq) transition in explicit solvent. The kinetic results for both systems in vacuum and explicit solvent are in general agreement with previous experimental and computational studies for this peptide. In vacuum, an additional dihedral angle besides the one typically used as order parameter is identified as a significant variable in the reaction coordinate model. In solution, several dihedral angles and variables that describe the solvent action on the molecule's dynamics are found to play a significant role in the description of the system's dynamics.
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kinetics and reaction coordinate for the isomerization of alanine dipeptide by a forward flux Sampling Protocol
Journal of Chemical Physics, 2009Co-Authors: Camilo Velezvega, Ernesto E Borrero, Fernando A EscobedoAbstract:Forward flux Sampling (FFS) simulations were used to study the kinetics of alanine dipeptide both in vacuum and in explicit solvent. The recently proposed FFS least-squares estimation approach and an algorithm that optimizes the position of the interfaces were implemented to determine a reaction coordinate that adequately describes the transition dynamics. A new method is also introduced to try to ensure that the ensemble of “starting points” (for the trial trajectories) is properly sampled. The rate constant estimates for the C7eq⇒C5 transition of alanine dipeptide in vacuum were used to demonstrate the consistency between Monte Carlo and molecular dynamics (MD) simulations. FFS-MD simulations were then performed for the study of the β2/αR⇒C5/C7eq transition in explicit solvent. The kinetic results for both systems in vacuum and explicit solvent are in general agreement with previous experimental and computational studies for this peptide. In vacuum, an additional dihedral angle besides the one typically...
Sharif S Aly - One of the best experts on this subject based on the ideXlab platform.
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environmental Sampling to assess the bioburden of mycobacterium avium subspecies paratuberculosis in drylot pens on california dairies
PeerJ, 2019Co-Authors: Tapakorn Chamchoy, Deneice R Williams, John M Adaska, Randall J Anderson, Sharif S AlyAbstract:Mycobacterium avium subspecies paratuberculosis (MAP) is a bacterium that can cause substantial economic losses in infected dairy herds due to reduced milk production and increased cow-replacement costs. In order to control MAP in dairies with drylot pens, a standardized environmental Sampling Protocol to quantify MAP in fecal slurry was developed based on an existing Protocol for freestall pens. Specifically, following a 24 h hold of the flush, a grab sample of approximately 10 ml of fecal slurry was collected every 1 m along the flush lane of the drylot pens, avoiding individual cow fecal pats. To determine the reliability and repatability of the new environmental Sampling Protocol for estimation of MAP bioburden at the pen level, two collectors simultaneously collected fecal slurry samples every day for 3 days from six drylot cow pens on two Central California dairies. During the study period no cow movement between pens was allowed with the exception of sick cows. The study herds had MAP seroprevalence of 5.8% and 3.2%, respectively, based on whole pen serum ELISA results. Variance components models for quantitative real-time PCR (qPCR) results showed samples collected from different pens on different dairies accounted for greater variablitiy in MAP concentration (65%), while samples collected by different collectors had the least variability (0.1%). In contrast, variability in MAP concentration in environmental samples collected on different days had 25% variability. The intraclass correlation coefficient showed high reliability (93%) of environmental Sampling simultaneously by different collectors. In contrast, the reliability of environmental Sampling at different days was 65%, which was similar to the reliability for Sampling by different collectors on different days. Investigators can expect high reliability when employing the new environmental Sampling Protocol along with qPCR testing of environmental samples from drylot pens.
John M Adaska - One of the best experts on this subject based on the ideXlab platform.
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environmental Sampling to assess the bioburden of mycobacterium avium subspecies paratuberculosis in drylot pens on california dairies
PeerJ, 2019Co-Authors: Tapakorn Chamchoy, Deneice R Williams, John M Adaska, Randall J AndersonAbstract:Author(s): Chamchoy, Tapakorn; Williams, Deneice R; Adaska, John M; Anderson, Randall J; Aly, Sharif S | Abstract: Mycobacterium avium subspecies paratuberculosis (MAP) is a bacterium that can cause substantial economic losses in infected dairy herds due to reduced milk production and increased cow-replacement costs. In order to control MAP in dairies with drylot pens, a standardized environmental Sampling Protocol to quantify MAP in fecal slurry was developed based on an existing Protocol for freestall pens. Specifically, following a 24 h hold of the flush, a grab sample of approximately 10 ml of fecal slurry was collected every 1 m along the flush lane of the drylot pens, avoiding individual cow fecal pats. To determine the reliability and repatability of the new environmental Sampling Protocol for estimation of MAP bioburden at the pen level, two collectors simultaneously collected fecal slurry samples every day for 3 days from six drylot cow pens on two Central California dairies. During the study period no cow movement between pens was allowed with the exception of sick cows. The study herds had MAP seroprevalence of 5.8% and 3.2%, respectively, based on whole pen serum ELISA results. Variance components models for quantitative real-time PCR (qPCR) results showed samples collected from different pens on different dairies accounted for greater variablitiy in MAP concentration (65%), while samples collected by different collectors had the least variability (0.1%). In contrast, variability in MAP concentration in environmental samples collected on different days had 25% variability. The intraclass correlation coefficient showed high reliability (93%) of environmental Sampling simultaneously by different collectors. In contrast, the reliability of environmental Sampling at different days was 65%, which was similar to the reliability for Sampling by different collectors on different days. Investigators can expect high reliability when employing the new environmental Sampling Protocol along with qPCR testing of environmental samples from drylot pens.
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environmental Sampling to assess the bioburden of mycobacterium avium subspecies paratuberculosis in drylot pens on california dairies
PeerJ, 2019Co-Authors: Tapakorn Chamchoy, Deneice R Williams, John M Adaska, Randall J Anderson, Sharif S AlyAbstract:Mycobacterium avium subspecies paratuberculosis (MAP) is a bacterium that can cause substantial economic losses in infected dairy herds due to reduced milk production and increased cow-replacement costs. In order to control MAP in dairies with drylot pens, a standardized environmental Sampling Protocol to quantify MAP in fecal slurry was developed based on an existing Protocol for freestall pens. Specifically, following a 24 h hold of the flush, a grab sample of approximately 10 ml of fecal slurry was collected every 1 m along the flush lane of the drylot pens, avoiding individual cow fecal pats. To determine the reliability and repatability of the new environmental Sampling Protocol for estimation of MAP bioburden at the pen level, two collectors simultaneously collected fecal slurry samples every day for 3 days from six drylot cow pens on two Central California dairies. During the study period no cow movement between pens was allowed with the exception of sick cows. The study herds had MAP seroprevalence of 5.8% and 3.2%, respectively, based on whole pen serum ELISA results. Variance components models for quantitative real-time PCR (qPCR) results showed samples collected from different pens on different dairies accounted for greater variablitiy in MAP concentration (65%), while samples collected by different collectors had the least variability (0.1%). In contrast, variability in MAP concentration in environmental samples collected on different days had 25% variability. The intraclass correlation coefficient showed high reliability (93%) of environmental Sampling simultaneously by different collectors. In contrast, the reliability of environmental Sampling at different days was 65%, which was similar to the reliability for Sampling by different collectors on different days. Investigators can expect high reliability when employing the new environmental Sampling Protocol along with qPCR testing of environmental samples from drylot pens.
