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Mitsuo Ochi - One of the best experts on this subject based on the ideXlab platform.
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feasibility of prefabricated vascularized bone graft using the combination of fgf 2 and vascular bundle implantation within hydroxyapatite for osteointegration
Journal of Biomedical Materials Research Part A, 2008Co-Authors: Tomoyuki Nakasa, Osamu Ishida, Toru Sunagawa, Atsuo Nakamae, Kazunori Yokota, Nobuo Adachi, Mitsuo OchiAbstract:The aim of this study was to demonstrate the feasibility of the prefabricated vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic (IP-CHA) in combination with vascular bundle implantation and basic fibroblast growth factor (FGF-2) administration in rabbit model. Thirty Japanese white rabbits were used. To make a prefabricated bone graft, the Saphenous Artery and vein were passed through the hole of the IP-CHA. Hundred micrograms of FGF-2 was administered into the IP-CHA before implanting the vascular bundle. First and foremost, the IP-CHA was placed subcutaneously in the medial thigh of rabbits for 4 weeks. In the experimental group, a prefabricated vascularized bone graft was used while IP-CHA alone was used in the control group. Second, the prefabricated vascularized bone graft was transplanted from the subcutaneous implanted site into the medial femoral condyle defect of the same rabbit and IP-CHA alone was implanted as the control graft in a different animal. At 4 weeks posttransplantation, bone union with host bone could be observed in the experimental group. However, the area of bone formation of the control group was significantly higher than in the experimental at 2 and 4 weeks posttransplantation. We conclude that the prefabricated vascularized bone graft when transplanted into a bone defect showed the ability for bone union with the host bone, although further studies are needed to accelerate the process of bone formation.
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prefabrication of vascularized bone graft using a combination of fibroblast growth factor 2 and vascular bundle implantation into a novel interconnected porous calcium hydroxyapatite ceramic
Journal of Biomedical Materials Research Part A, 2005Co-Authors: Tomoyuki Nakasa, Osamu Ishida, Toru Sunagawa, Atsuo Nakamae, Yuji Yasunaga, Muhammad Agung, Mitsuo OchiAbstract:The aim of this study was to create a prefabricated vascularized bone graft using a novel interconnected porous calcium hydroxyapatite ceramic (IP-CHA) by combining vascular bundle implantation and basic fibroblast growth factor (FGF)-2 administration in a rabbit model. Twenty-four Japanese white rabbits were used. The Saphenous Artery and vein were passed through the hole of the IP-CHA. In an experimental group, 100 μg of FGF-2 was administered into the IP-CHA before implanting the vascular bundle. In the control group, the saline was administered into the IP-CHA before implanting the vascular bundle. Finally, the IP-CHA was placed subcutaneously in the medial thigh. Neovascularization from the vascular bundle was evaluated at 2 weeks after surgery, and osteogenesis was evaluated at 4 weeks. At 2 weeks, the length and density of newly formed vessels were significantly greater in the experimental group than in the control group. Histological evaluation showed osteoid deposition in the pores of the IP-CHA at 4 weeks in the experimental group, whereas no evidence of osteoid deposition was noted in the control group. This study showed the potential of creating a vascularized bone graft of a predetermined size and shape using a combination of FGF-2 and vascular bundle implantation in the IP-CHA. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
Tomoyuki Nakasa - One of the best experts on this subject based on the ideXlab platform.
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feasibility of prefabricated vascularized bone graft using the combination of fgf 2 and vascular bundle implantation within hydroxyapatite for osteointegration
Journal of Biomedical Materials Research Part A, 2008Co-Authors: Tomoyuki Nakasa, Osamu Ishida, Toru Sunagawa, Atsuo Nakamae, Kazunori Yokota, Nobuo Adachi, Mitsuo OchiAbstract:The aim of this study was to demonstrate the feasibility of the prefabricated vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic (IP-CHA) in combination with vascular bundle implantation and basic fibroblast growth factor (FGF-2) administration in rabbit model. Thirty Japanese white rabbits were used. To make a prefabricated bone graft, the Saphenous Artery and vein were passed through the hole of the IP-CHA. Hundred micrograms of FGF-2 was administered into the IP-CHA before implanting the vascular bundle. First and foremost, the IP-CHA was placed subcutaneously in the medial thigh of rabbits for 4 weeks. In the experimental group, a prefabricated vascularized bone graft was used while IP-CHA alone was used in the control group. Second, the prefabricated vascularized bone graft was transplanted from the subcutaneous implanted site into the medial femoral condyle defect of the same rabbit and IP-CHA alone was implanted as the control graft in a different animal. At 4 weeks posttransplantation, bone union with host bone could be observed in the experimental group. However, the area of bone formation of the control group was significantly higher than in the experimental at 2 and 4 weeks posttransplantation. We conclude that the prefabricated vascularized bone graft when transplanted into a bone defect showed the ability for bone union with the host bone, although further studies are needed to accelerate the process of bone formation.
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prefabrication of vascularized bone graft using a combination of fibroblast growth factor 2 and vascular bundle implantation into a novel interconnected porous calcium hydroxyapatite ceramic
Journal of Biomedical Materials Research Part A, 2005Co-Authors: Tomoyuki Nakasa, Osamu Ishida, Toru Sunagawa, Atsuo Nakamae, Yuji Yasunaga, Muhammad Agung, Mitsuo OchiAbstract:The aim of this study was to create a prefabricated vascularized bone graft using a novel interconnected porous calcium hydroxyapatite ceramic (IP-CHA) by combining vascular bundle implantation and basic fibroblast growth factor (FGF)-2 administration in a rabbit model. Twenty-four Japanese white rabbits were used. The Saphenous Artery and vein were passed through the hole of the IP-CHA. In an experimental group, 100 μg of FGF-2 was administered into the IP-CHA before implanting the vascular bundle. In the control group, the saline was administered into the IP-CHA before implanting the vascular bundle. Finally, the IP-CHA was placed subcutaneously in the medial thigh. Neovascularization from the vascular bundle was evaluated at 2 weeks after surgery, and osteogenesis was evaluated at 4 weeks. At 2 weeks, the length and density of newly formed vessels were significantly greater in the experimental group than in the control group. Histological evaluation showed osteoid deposition in the pores of the IP-CHA at 4 weeks in the experimental group, whereas no evidence of osteoid deposition was noted in the control group. This study showed the potential of creating a vascularized bone graft of a predetermined size and shape using a combination of FGF-2 and vascular bundle implantation in the IP-CHA. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
Yuehong Zhuang - One of the best experts on this subject based on the ideXlab platform.
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segmental branches emanating from Saphenous nerve morphing into sympathetic trunks for innervation of Saphenous Artery and its clinical implication for arterial sympathectomy
International Wound Journal, 2021Co-Authors: Yun Xie, Fang Fang, Peisen Lin, Zhiming Zhang, Yuehong ZhuangAbstract:Sympathectomy of arteries has been adopted for the treatment of peripheral arterial disease and Raynaud's disease. However, the exact route for sympathetic axons to reach peripheral arteries awaits further investigation that could pave the way for development of new surgical strategies. In this study, Saphenous neurovascular bundles from 10 neonatal Sprague-Dawley rats first were harvested for whole-mount immunostaining to show sympathetic innervation pattern of the Artery. Secondly, 40 Sprague-Dawley male rats weighing 350 to 400 g were assigned to five groups, receiving either sham, perivascular sympathectomy, nerve-Artery separation, nerve transection in the Saphenous neurovascular bundle, or lumbar sympathectomy surgery that removes the lumbar sympathetic trunks. Immediately after surgery, the arterial perfusion and diameter were measured using laser speckling contrast imaging, and 1 week later the Saphenous neurovascular bundles were harvested for immunostaining using antibodies against TH, neuron-specific β-tubulin (Tuj 1), and α-SMA to show the presence or absence of the TH-immuopositive staining in the adventitia. The differences among the five groups were determined using one-way analysis of variance (ANOVA). We found that an average of 2.8 ± 0.8 branches with a diameter of 4.8 ± 1.2 μm derived from the Saphenous nerve that morphed into a primary and a secondary sympathetic trunk for innervation of the Saphenous Artery. Nerve-Artery separation, nerve transection, and lumbar sympathectomy could eradicate TH-immunopositive staining of the Artery, resulting, respectively, in a 12%, 36%, and 59% increase in diameter (P .05). We conclude that the sympathetic innervation of an Artery derives from segmental branches given off from its accompanying nerve. Nerve-Artery disconnection is a theoretic option in sympathectomy of an Artery.
Osamu Ishida - One of the best experts on this subject based on the ideXlab platform.
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feasibility of prefabricated vascularized bone graft using the combination of fgf 2 and vascular bundle implantation within hydroxyapatite for osteointegration
Journal of Biomedical Materials Research Part A, 2008Co-Authors: Tomoyuki Nakasa, Osamu Ishida, Toru Sunagawa, Atsuo Nakamae, Kazunori Yokota, Nobuo Adachi, Mitsuo OchiAbstract:The aim of this study was to demonstrate the feasibility of the prefabricated vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic (IP-CHA) in combination with vascular bundle implantation and basic fibroblast growth factor (FGF-2) administration in rabbit model. Thirty Japanese white rabbits were used. To make a prefabricated bone graft, the Saphenous Artery and vein were passed through the hole of the IP-CHA. Hundred micrograms of FGF-2 was administered into the IP-CHA before implanting the vascular bundle. First and foremost, the IP-CHA was placed subcutaneously in the medial thigh of rabbits for 4 weeks. In the experimental group, a prefabricated vascularized bone graft was used while IP-CHA alone was used in the control group. Second, the prefabricated vascularized bone graft was transplanted from the subcutaneous implanted site into the medial femoral condyle defect of the same rabbit and IP-CHA alone was implanted as the control graft in a different animal. At 4 weeks posttransplantation, bone union with host bone could be observed in the experimental group. However, the area of bone formation of the control group was significantly higher than in the experimental at 2 and 4 weeks posttransplantation. We conclude that the prefabricated vascularized bone graft when transplanted into a bone defect showed the ability for bone union with the host bone, although further studies are needed to accelerate the process of bone formation.
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prefabrication of vascularized bone graft using a combination of fibroblast growth factor 2 and vascular bundle implantation into a novel interconnected porous calcium hydroxyapatite ceramic
Journal of Biomedical Materials Research Part A, 2005Co-Authors: Tomoyuki Nakasa, Osamu Ishida, Toru Sunagawa, Atsuo Nakamae, Yuji Yasunaga, Muhammad Agung, Mitsuo OchiAbstract:The aim of this study was to create a prefabricated vascularized bone graft using a novel interconnected porous calcium hydroxyapatite ceramic (IP-CHA) by combining vascular bundle implantation and basic fibroblast growth factor (FGF)-2 administration in a rabbit model. Twenty-four Japanese white rabbits were used. The Saphenous Artery and vein were passed through the hole of the IP-CHA. In an experimental group, 100 μg of FGF-2 was administered into the IP-CHA before implanting the vascular bundle. In the control group, the saline was administered into the IP-CHA before implanting the vascular bundle. Finally, the IP-CHA was placed subcutaneously in the medial thigh. Neovascularization from the vascular bundle was evaluated at 2 weeks after surgery, and osteogenesis was evaluated at 4 weeks. At 2 weeks, the length and density of newly formed vessels were significantly greater in the experimental group than in the control group. Histological evaluation showed osteoid deposition in the pores of the IP-CHA at 4 weeks in the experimental group, whereas no evidence of osteoid deposition was noted in the control group. This study showed the potential of creating a vascularized bone graft of a predetermined size and shape using a combination of FGF-2 and vascular bundle implantation in the IP-CHA. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
Toru Sunagawa - One of the best experts on this subject based on the ideXlab platform.
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feasibility of prefabricated vascularized bone graft using the combination of fgf 2 and vascular bundle implantation within hydroxyapatite for osteointegration
Journal of Biomedical Materials Research Part A, 2008Co-Authors: Tomoyuki Nakasa, Osamu Ishida, Toru Sunagawa, Atsuo Nakamae, Kazunori Yokota, Nobuo Adachi, Mitsuo OchiAbstract:The aim of this study was to demonstrate the feasibility of the prefabricated vascularized bone graft using an interconnected porous calcium hydroxyapatite ceramic (IP-CHA) in combination with vascular bundle implantation and basic fibroblast growth factor (FGF-2) administration in rabbit model. Thirty Japanese white rabbits were used. To make a prefabricated bone graft, the Saphenous Artery and vein were passed through the hole of the IP-CHA. Hundred micrograms of FGF-2 was administered into the IP-CHA before implanting the vascular bundle. First and foremost, the IP-CHA was placed subcutaneously in the medial thigh of rabbits for 4 weeks. In the experimental group, a prefabricated vascularized bone graft was used while IP-CHA alone was used in the control group. Second, the prefabricated vascularized bone graft was transplanted from the subcutaneous implanted site into the medial femoral condyle defect of the same rabbit and IP-CHA alone was implanted as the control graft in a different animal. At 4 weeks posttransplantation, bone union with host bone could be observed in the experimental group. However, the area of bone formation of the control group was significantly higher than in the experimental at 2 and 4 weeks posttransplantation. We conclude that the prefabricated vascularized bone graft when transplanted into a bone defect showed the ability for bone union with the host bone, although further studies are needed to accelerate the process of bone formation.
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prefabrication of vascularized bone graft using a combination of fibroblast growth factor 2 and vascular bundle implantation into a novel interconnected porous calcium hydroxyapatite ceramic
Journal of Biomedical Materials Research Part A, 2005Co-Authors: Tomoyuki Nakasa, Osamu Ishida, Toru Sunagawa, Atsuo Nakamae, Yuji Yasunaga, Muhammad Agung, Mitsuo OchiAbstract:The aim of this study was to create a prefabricated vascularized bone graft using a novel interconnected porous calcium hydroxyapatite ceramic (IP-CHA) by combining vascular bundle implantation and basic fibroblast growth factor (FGF)-2 administration in a rabbit model. Twenty-four Japanese white rabbits were used. The Saphenous Artery and vein were passed through the hole of the IP-CHA. In an experimental group, 100 μg of FGF-2 was administered into the IP-CHA before implanting the vascular bundle. In the control group, the saline was administered into the IP-CHA before implanting the vascular bundle. Finally, the IP-CHA was placed subcutaneously in the medial thigh. Neovascularization from the vascular bundle was evaluated at 2 weeks after surgery, and osteogenesis was evaluated at 4 weeks. At 2 weeks, the length and density of newly formed vessels were significantly greater in the experimental group than in the control group. Histological evaluation showed osteoid deposition in the pores of the IP-CHA at 4 weeks in the experimental group, whereas no evidence of osteoid deposition was noted in the control group. This study showed the potential of creating a vascularized bone graft of a predetermined size and shape using a combination of FGF-2 and vascular bundle implantation in the IP-CHA. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
