The Experts below are selected from a list of 3201 Experts worldwide ranked by ideXlab platform
Jian Wu Wang - One of the best experts on this subject based on the ideXlab platform.
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design synthesis and antibacterial activity of novel actinonin derivatives containing benzimidazole heterocycles
European Journal of Medicinal Chemistry, 2009Co-Authors: Datong Zhang, Zongheng Wang, Wei Ren Xu, Lida Tang, Jian Wu WangAbstract:Abstract A series of novel actinonin derivatives containing a benzimidazole heterocycle linked as amide isostere have been designed and synthesized. The structures of all the synthesized compounds were confirmed by analytical and spectroscopic methods. All the compounds were evaluated in vitro against Staphylococcus aureus , Klebsiella pneumoniae , and Sarcina lutea . Among them, compound 1a with unsubstituted benzimidazole ring exhibited potent antibacterial activities.
Carl C Correll - One of the best experts on this subject based on the ideXlab platform.
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The electrostatic character of the ribosomal surface enables extraordinarily rapid target location by ribotoxins
Nature Structural & Molecular Biology, 2006Co-Authors: Alexei V Korennykh, Joseph A Piccirilli, Carl C CorrellAbstract:α-sarcin ribotoxins comprise a unique family of ribonucleases that cripple the ribosome by catalyzing endoribonucleolytic cleavage of ribosomal RNA at a specific location in the sarcin/ricin loop (SRL). The SRL structure alone is cleaved site-specifically by the ribotoxin, but the ribosomal context enhances the reaction rate by several orders of magnitude. We show that, for the α-sarcin–like ribotoxin restrictocin, this catalytic advantage arises from favorable electrostatic interactions with the ribosome. Restrictocin binds at many sites on the ribosomal surface and under certain conditions cleaves the SRL with a second-order rate constant of 1.7 × 10^10 M^−1 s^−1, a value that matches the predicted frequency of random restrictocin-ribosome encounters. The results suggest a mechanism of target location whereby restrictocin encounters ribosomes randomly and diffuses within the ribosomal electrostatic field to the SRL. These studies show a role for electrostatics in protein-ribosome recognition.
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Crystal structures of restrictocin–inhibitor complexes with implications for RNA recognition and base flipping
Nature Structural Biology, 2001Co-Authors: Xiaojing Yang, Tímea Gérczei, Latonya Glover, Carl C CorrellAbstract:The cytotoxin sarcin disrupts elongation factor binding and protein synthesis by specifically cleaving one phosphodiester bond in ribosomes. To elucidate the molecular basis of toxin action, we determined three cocrystal structures of the sarcin homolog restrictocin bound to different analogs that mimic the target sarcin/ricin loop (SRL) structure of the rat 28S rRNA. In these structures, restrictocin contacts the bulged-G motif and an unfolded form of the tetraloop of the SRL RNA. In one structure, toxin loops guide selection of the target site by contacting the base critical for recognition (G4319) and the surrounding S-shaped backbone. In another structure, base flipping of the tetraloop enables cleavage by placing the target nucleotide in the active site with the nucleophile nearly inline for attack on the scissile bond. These structures provide the first views of how a site-specific protein endonuclease recognizes and cleaves a folded RNA substrate.
Datong Zhang - One of the best experts on this subject based on the ideXlab platform.
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design synthesis and antibacterial activity of novel actinonin derivatives containing benzimidazole heterocycles
European Journal of Medicinal Chemistry, 2009Co-Authors: Datong Zhang, Zongheng Wang, Wei Ren Xu, Lida Tang, Jian Wu WangAbstract:Abstract A series of novel actinonin derivatives containing a benzimidazole heterocycle linked as amide isostere have been designed and synthesized. The structures of all the synthesized compounds were confirmed by analytical and spectroscopic methods. All the compounds were evaluated in vitro against Staphylococcus aureus , Klebsiella pneumoniae , and Sarcina lutea . Among them, compound 1a with unsubstituted benzimidazole ring exhibited potent antibacterial activities.
M. Hofshagen - One of the best experts on this subject based on the ideXlab platform.
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Sarcina like bacteria clostridium fallax and clostridium sordellii in lambs with abomasal bloat haemorrhage and ulcers
Journal of Comparative Pathology, 2000Co-Authors: S. Vatn, M.a. Tranulis, M. HofshagenAbstract:A study of abomasal disease in lambs aged 2-5 weeks, made during the period 1993-1998, included 67 cases and 45 non-affected controls. Gross pathological findings included various combinations of bloat, haemorrhage and ulcers in the abomasum. Sarcina -like bacteria were found in sections and smears from the abomasum of 79% (53/67) of the cases. From one case, a lamb with abomasal bloat, the anaerobic "packet"-forming Sarcina ventriculi was cultivated from the abomasal contents and identified by biochemical reactions and sequencing of the 16S rRNA gene. Sarcina -like bacteria were observed microscopically in specimens from 94% (44/47) of the lambs with abomasal gas and in 45% (9/20) of those with ulcers or haemorrhage or both but little gas. On culture, abomasal contents from 41 cases yielded Clostridium fallax from 16 (39%) and Clostridium sordellii from eight (20%); abomasal cultures from 30 control lambs were negative for the three bacterial species. Quantitative cultivation, carried out on abomasal contents from live lambs and lambs dead
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Sarcina -like bacteria, Clostridium fallax and Clostridium sordellii in lambs with abomasal bloat, haemorrhage and ulcers.
Journal of Comparative Pathology, 2000Co-Authors: S. Vatn, M.a. Tranulis, M. HofshagenAbstract:A study of abomasal disease in lambs aged 2-5 weeks, made during the period 1993-1998, included 67 cases and 45 non-affected controls. Gross pathological findings included various combinations of bloat, haemorrhage and ulcers in the abomasum. Sarcina -like bacteria were found in sections and smears from the abomasum of 79% (53/67) of the cases. From one case, a lamb with abomasal bloat, the anaerobic "packet"-forming Sarcina ventriculi was cultivated from the abomasal contents and identified by biochemical reactions and sequencing of the 16S rRNA gene. Sarcina -like bacteria were observed microscopically in specimens from 94% (44/47) of the lambs with abomasal gas and in 45% (9/20) of those with ulcers or haemorrhage or both but little gas. On culture, abomasal contents from 41 cases yielded Clostridium fallax from 16 (39%) and Clostridium sordellii from eight (20%); abomasal cultures from 30 control lambs were negative for the three bacterial species. Quantitative cultivation, carried out on abomasal contents from live lambs and lambs dead
Gizem Ayaz - One of the best experts on this subject based on the ideXlab platform.
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Postmortem Diagnosis of Gastric Ulcer Perforation and Peritonitis with Sarcina Ventriculi: A Case Report: Sarcina Ventriculi Infection
The Bulletin of Legal Medicine, 2019Co-Authors: Aytül Buğra, Taner Daş, Neval Elgörmüş, Gizem AyazAbstract:Sarcina ventriculi is a gram-positive anaerobic coccus with characteristic tetrad morphology. Sarcina ventriculiis identified by light microscopy with features of basophilic staining, cuboidal shape, tetrad morphology, and refractile nature. There have been very few case reports of Sarcina ventriculi reported in the literature. We present a case of a 53-year-old male, with gastric ulcer perforation where peritonitis was incidentally found to harbor Sarcina ventriculi in postmortem histopathological examination. Most of the cases exhibit abdominal pain, nausea, vomiting and delayed gastric emptying. It has also fatal life-threatening complications, such as gastric perforation and emphysematous gastritis. The histopathological examination has a key role for identification of the bacteria. The pathologist must always keep it in mind these bacteria as a cause of gastric ulcer perforation in the differential diagnosis. We want to present a case of a 53-year-old male gastric ulcer perforation who was found dead in his bed.
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Postmortem Diagnosis of Gastric Ulcer Perforation and Peritonitis with Sarcina Ventriculi: A Case Report
Adli Tıp Uzmanları Derneği, 2019Co-Authors: Aytül Buğra, Taner Daş, Neval Elgörmüş, Gizem AyazAbstract:Sarcina ventriculi is a gram-positive anaerobic coccus with characteristic tetrad morphology. Sarcina ventriculiis identified by light microscopy with features of basophilic staining, cuboidal shape, tetrad morphology, and refractile nature. There have been very few case reports of Sarcina ventriculi reported in the literature. We present a case of a 53-year-old male, with gastric ulcer perforation where peritonitis was incidentally found to harbor Sarcina ventriculi in postmortem histopathological examination. Most of the cases exhibit abdominal pain, nausea, vomiting and delayed gastric emptying. It has also fatal life-threatening complications, such as gastric perforation and emphysematous gastritis. The histopathological examination has a key role for identification of the bacteria. The pathologist must always keep it in mind these bacteria as a cause of gastric ulcer perforation in the differential diagnosis. We want to present a case of a 53-year-old male gastric ulcer perforation who was found dead in his bed
