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P T Thomas - One of the best experts on this subject based on the ideXlab platform.
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in vitro oxygen consumption and motility of cryptobia salmositica cryptobia bullocki and cryptobia catostomi Sarcomastigophora kinetoplastida
Journal of Parasitology, 1992Co-Authors: P T Thomas, James S Ballantyne, P.t.k. WooAbstract:Cryptobia salmositica (pathogenic and vaccine strains), Cryptobia bullocki (pathogenic), and Cryptobia catostomi (nonpathogenic) have similar oxygen consumption rates (0.17 +/- 0.01 nm O2/10(6) parasites). Incubation with sodium azide (5 microliters of a 1-M solution to 1 ml of parasite suspension, i.e., a 5-mM final concentration) reduced the oxygen consumption by approximately 4.5-fold. Motility of the parasites was also greatly reduced in sodium azide. The oxygen consumption and motility of the parasites returned to preazide treatment levels when the azide was removed even after 24 hr of incubation in sodium azide. The activities of hexokinase, pyruvate kinase, and cytochrome C oxidase were not detected in the 3 species of Cryptobia.
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comparative in vitro studies on virulent and avirulent strains of cryptobia salmositica Sarcomastigophora kinetoplastida
Journal of Fish Diseases, 1992Co-Authors: P T ThomasAbstract:. The optimum temperature for in vitro multiplication of Cryptobia salmositica was 10°C. The avirulent strain multiplied more rapidly than the virulent strain. The haemolytic components, lytic component (LC) and immune complex-forming component (ICC), were secreted by the two strains into the culture medium and were detectable from one week post-inoculation. The haemolytic activity in the supernatant increased with increasing parasite numbers in both strains. Although cultures of the avirulent strain had higher parasite numbers than those of the virulent strain, the haemolytic activity was significantly lower than that of the virulent strain. Antiserum against ICC was produced in rabbit by immunization with ICC-coated rainbow trout red blood cells.
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In vitro and in vivo effects of antimicrobial agents on viability of Cryptobia salmositica (Sarcomastigophora: Kinetoplastida).
Diseases of Aquatic Organisms, 1991Co-Authors: P T Thomas, P.t.k. WooAbstract:Cryptobia salmositica (Sarcomastigophora: Kinetoplastida) from blood of infected rainbow trout (blood forms) or cultures (culture forms) rounded up when incubated in vitro with a combination of Penicillin, Streptomycin and Arnphotericin B (antimicrobial agents, AM). Trypan blue dye exclusion test showed that slender forms did not take up the dye whereas many round forms became coloured as incubation proceeded. Culture forms were more susceptible to AM than blood forms. Washing the blood forms prior to incubation with AM increased their susceptibility, but they were still more resistant than unwashed or washed culture forms. Trypan blue had a protective effect on viability of both blood and culture forms exposed to AM. Parasites exposed to AM were still infective when inoculated into rainbow trout. Injection of AM into fish infected with blood or culture forms did not affect the parasitaemias. Although C. salmositica and trypanosomes are phylogenetically related, AM did not affect trypano- somes (mammalian and piscine).
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polypeptide and antigen profiles of cryptobia salmositica c bullocki and c catostomi kinetoplastida Sarcomastigophora isolated from fishes
Diseases of Aquatic Organisms, 1991Co-Authors: P T K Woo, P T ThomasAbstract:Polypeptide and antigen profiles of Cryptobia salmositica (virulent and avirulent strains). C. bullocki and C. catostomi were compared using SDS-PAGE and Western immunoblot. The avirulent strain of C. salmositica had 5 fewer bands than the virulent strain (21 bands) which is, perhaps, related to loss of virulence. C. catostomi had the highest number of bands (23) and a different banding pattern than the other 2 species. Although the 3 species had several bands of similar molecular mass, they were antigenically distinct. The avirulent and virulent strains of C. saln~ositica had several cross-reacting bands when probed with antiserum against the virulent strain. There were, however, no cross-reactions between C. salmositica and the other 2 species except for a band of 66 kD. We tentatively suggest that pathogenic and non-pathogenic species of Cryptobia have different polypeptide profiles and are antigenically distinct. SDS-PAGE followed by Western immunoblot may be a useful procedure to distinguish species of Cryptobia from various hosts and also to determine their phylogenetic relationships.
S Herrera - One of the best experts on this subject based on the ideXlab platform.
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entamoeba struthionis n sp Sarcomastigophora endamoebidae from ostriches struthio camelus
Veterinary Parasitology, 2004Co-Authors: Ponce F Gordo, R Martinez A Diaz, S HerreraAbstract:In the present work we identify the species of Entamoeba from ostriches (Struthio camelus). The complete sequence of the small subunit ribosomal RNA gene from this organism has been compared with those published for other species of the genus and clear differences have been found. These results confirm previous data which showed differences on parasite morphology and class of host with the other Entamoeba species. Taking all these data together, it can be concluded that the organism from ostriches is a new species whose proposed name is Entamoeba struthionis n.sp. This species probably infects rheas (Rhea americana), but genetic analysis of isolates from this host should be performed to confirm morphological data. Also, comparison of gene sequences with data from other authors on cysts recovered from human stool samples showed the possibility that this amoeba may affect humans. Further studies are needed to determine the risk of transmission of this new species to humans.
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entamoeba sp Sarcomastigophora endamoebidae from ostriches struthio camelus aves struthionidae
Veterinary Parasitology, 2000Co-Authors: Rafael A Martinezdiaz, S Herrera, A Castro, Francisco PonceAbstract:Abstract The first case of Entamoeba of the 1-nucleate mature cyst group in birds is described. Trophozoites and cysts of Entamoeba have been found in ostriches (Struthio camelus) from farms located all over Spain. The cysts are large (13.47 μm mean diameter); they possess one nucleus when mature, with a large endosome and peripheral chromatine arranged in small granules; chromatoid bodies, when present, are large and elongated. Trophozoites are large (19.88 μm mean diameter), with a clear differentiation between ecto- and endoplasm, this containing numerous vacuoles; the nucleus is large and diffuse. The characteristics of this amoeba resembles but do not completely fulfill those of E. suis and E. chattoni; also, these species are from mammals.
Francisco Ponce - One of the best experts on this subject based on the ideXlab platform.
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first report of the genus retortamonas Sarcomastigophora retortamonadidae in birds
Memorias Do Instituto Oswaldo Cruz, 2001Co-Authors: Rafael A Martinezdiaz, Ana Teresa Castro, Silvia Herrera, Francisco PonceAbstract:In studies carried out on the parasites infecting ostriches (Struthio camelus) in Spain, trophozoites of Retortamonas sp. have been found in the intestinal contents of 28 out of 146 slaughtered ostriches. The species infecting ostriches could not be determined from the morphological data available. However, these findings are important as they constitute the first report of the genus Retortamonas in birds.
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entamoeba sp Sarcomastigophora endamoebidae from ostriches struthio camelus aves struthionidae
Veterinary Parasitology, 2000Co-Authors: Rafael A Martinezdiaz, S Herrera, A Castro, Francisco PonceAbstract:Abstract The first case of Entamoeba of the 1-nucleate mature cyst group in birds is described. Trophozoites and cysts of Entamoeba have been found in ostriches (Struthio camelus) from farms located all over Spain. The cysts are large (13.47 μm mean diameter); they possess one nucleus when mature, with a large endosome and peripheral chromatine arranged in small granules; chromatoid bodies, when present, are large and elongated. Trophozoites are large (19.88 μm mean diameter), with a clear differentiation between ecto- and endoplasm, this containing numerous vacuoles; the nucleus is large and diffuse. The characteristics of this amoeba resembles but do not completely fulfill those of E. suis and E. chattoni; also, these species are from mammals.
Eiji Takahashi - One of the best experts on this subject based on the ideXlab platform.
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Choanoflagellates (Sarcomastigophora, Protozoa) from the Coastal Waters of Taiwan and Japan (II): Species Compcsition and Biogeography
1997Co-Authors: Seiko Hara, Jia-chi Sheu, Yuh-ling Lee Chen, Eiji TakahashiAbstract:Seiko Hara, Jia-Chi Sheu, Yuh-ling Lee Chen and Eiji Takahashi (1997) Choanoflagellates (Sarcomastigophora, Protozoa) from the coastal waters of Taiwan and Japan II. Species composition and biogeography. Zoological Studies 36(2): 98-110. Light and electron microscopical studies of sea microbes from the coastal waters of Taiwan have revealed an abundant choanoflagellate fauna from these Western Pacific subtropical waters. A total of 25 loricate (Acanthoecidae), 3 thecate (Salpingoecidae), and 1 naked (Codonosigidae) choanoflagellates were recorded for the tst time from coastal waters of Taiwan. The Taiwanese loricate choanoflagellate fauna is more similar to that of Japan (20 species in common) than to that of the tropical Indian Ocean (11 species in common).
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choanoflagellates Sarcomastigophora protozoa from the coastal waters of taiwan and japan i three new species
Journal of Eukaryotic Microbiology, 1996Co-Authors: Seiko Hara, Jia-chi Sheu, Yuh-ling Lee Chen, Eiji TakahashiAbstract:Three new acanthoecidaean species collected from the coastal waters of Taiwan and Japan are described : Acanthocorbis camarensis n. sp. resembles Acanthocorbis unguiculata in lorica morphology, but differs in having regularly arranged longitudinal and transverse costae at the anterior lorica chamber, and in lacking a nail at the apical end of anterior spine ; Diaphanoeca spiralifurca n. sp. is characterized by the spiral arrangement of the costal strips in the posterior half of the lorica chamber and is closely related to Diaphanoeca grandis ; Stephanoeca supracostata n. sp. is closely related to Stephanoeca elegans, but differs in having an additional transverse costa at the anterior lorica chamber.
Rafael A Martinezdiaz - One of the best experts on this subject based on the ideXlab platform.
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first report of the genus retortamonas Sarcomastigophora retortamonadidae in birds
Memorias Do Instituto Oswaldo Cruz, 2001Co-Authors: Rafael A Martinezdiaz, Ana Teresa Castro, Silvia Herrera, Francisco PonceAbstract:In studies carried out on the parasites infecting ostriches (Struthio camelus) in Spain, trophozoites of Retortamonas sp. have been found in the intestinal contents of 28 out of 146 slaughtered ostriches. The species infecting ostriches could not be determined from the morphological data available. However, these findings are important as they constitute the first report of the genus Retortamonas in birds.
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entamoeba sp Sarcomastigophora endamoebidae from ostriches struthio camelus aves struthionidae
Veterinary Parasitology, 2000Co-Authors: Rafael A Martinezdiaz, S Herrera, A Castro, Francisco PonceAbstract:Abstract The first case of Entamoeba of the 1-nucleate mature cyst group in birds is described. Trophozoites and cysts of Entamoeba have been found in ostriches (Struthio camelus) from farms located all over Spain. The cysts are large (13.47 μm mean diameter); they possess one nucleus when mature, with a large endosome and peripheral chromatine arranged in small granules; chromatoid bodies, when present, are large and elongated. Trophozoites are large (19.88 μm mean diameter), with a clear differentiation between ecto- and endoplasm, this containing numerous vacuoles; the nucleus is large and diffuse. The characteristics of this amoeba resembles but do not completely fulfill those of E. suis and E. chattoni; also, these species are from mammals.
