The Experts below are selected from a list of 3243 Experts worldwide ranked by ideXlab platform
Tea Vallenius - One of the best experts on this subject based on the ideXlab platform.
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Schematic Presentation of destabilized E-cadherin-based adhesions and associated actin fibers following increased α-actinin-1 expression.
2018Co-Authors: Bianca Kovac, Tomi P. Mäkelä, Tea ValleniusAbstract:Schematic Presentation of destabilized E-cadherin-based adhesions and associated actin fibers following increased α-actinin-1 expression.
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High α-actinin-1 expression in basal-like breast cancer cells destabilizes E-cadherin based adhesions.
2018Co-Authors: Bianca Kovac, Tomi P. Mäkelä, Tea ValleniusAbstract:(A) Western blotting analysis of MDA-MB-231 cells expressing either GFP (Control) or GFP-tagged E-cadherin (+ E-cadherin) in combination with siRNA mediated downregulation using non-targeting (siNT) or α-actinin-1 (siA1) oligos, as indicated. Dotted lines indicate removal of intervening lanes. (B) Merged immunofluorescence images of phalloidin (F-actin, green) stained MDA-MB-231 cells expressing GFP (Control) or GFP-E-cadherin (+ E-cadherin). GFP-signal is pseudo-colored to red, and Hoechst visualizes nuclei. The arrowhead points to punctate E-cadherin and arrows point to subcortical actin fibers. Scale bar, 10 μm (C) A representative example of a change from punctate to linear E-cadherin following α-actinin-1 downregulation (siA1) in MDA-MB-231 cells re-expressing E-cadherin. (D) Phalloidin (F-actin, green) and Hoechst (blue) co-stained HCC1937 cells following siRNA-mediated downregulation using non-targeting (siNT), α-actinin-1 (siA1) or α-actinin-4 (siA4) oligos, as indicated. Arrows show F-actin reorganization. Scale bar, 10 μm. (E) Zoom-in immunofluorescence images of E-cadherin or merged F-actin/E-cadherin following downregulation of control (siNT), α-actinin-1 (siA1) or α-actinin-4 (siA4), as indicated. (F) Immunofluorescence image of a wild-type HCC1937 cell stained for α-actinin-1 (purple) and merged image of α-actinin-1 (purple) and F-actin (green) to demonstrate the localization of endogenous α-actinin-1 on radial (white arrowheads) and arc-like (yellow arrows) actin fibers at E-cadherin based adhesion (see also Fig 5 Schematic Presentation). (G) Western blotting analysis with the indicated antibodies following downregulation of control (siNT), α-actinin-1 (siA1) or α-actinin-4 (siA4) in HCC1937 cells.
Reinhild Klein - One of the best experts on this subject based on the ideXlab platform.
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additional file 1 of sulphite oxidase so a mitochondrial autoantigen as target for humoral and cellular immune reactions in primary sclerosing cholangitis
2018Co-Authors: Beate Preus, Christoph P Berg, C R Werner, Sandra Plankenhorn, Nisar P Malek, Reinhild KleinAbstract:Schematic Presentation of the enzyme sulphite oxidase (SO) with its different domains, and the immuno-dominant epitopes recognised by PSC sera (aa = amino acid; mo = molybdenum binding site). (PDF 165 kb)
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Additional file 1: of Sulphite oxidase (SO) – a mitochondrial autoantigen as target for humoral and cellular immune reactions in primary sclerosing cholangitis
2018Co-Authors: Beate Preuß, Sandra Plankenhorn, Christoph Berg, Christoph Werner, Nisar Malek, Reinhild KleinAbstract:Schematic Presentation of the enzyme sulphite oxidase (SO) with its different domains, and the immuno-dominant epitopes recognised by PSC sera (aa = amino acid; mo = molybdenum binding site). (PDF 165 kb
Bianca Kovac - One of the best experts on this subject based on the ideXlab platform.
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Schematic Presentation of destabilized E-cadherin-based adhesions and associated actin fibers following increased α-actinin-1 expression.
2018Co-Authors: Bianca Kovac, Tomi P. Mäkelä, Tea ValleniusAbstract:Schematic Presentation of destabilized E-cadherin-based adhesions and associated actin fibers following increased α-actinin-1 expression.
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High α-actinin-1 expression in basal-like breast cancer cells destabilizes E-cadherin based adhesions.
2018Co-Authors: Bianca Kovac, Tomi P. Mäkelä, Tea ValleniusAbstract:(A) Western blotting analysis of MDA-MB-231 cells expressing either GFP (Control) or GFP-tagged E-cadherin (+ E-cadherin) in combination with siRNA mediated downregulation using non-targeting (siNT) or α-actinin-1 (siA1) oligos, as indicated. Dotted lines indicate removal of intervening lanes. (B) Merged immunofluorescence images of phalloidin (F-actin, green) stained MDA-MB-231 cells expressing GFP (Control) or GFP-E-cadherin (+ E-cadherin). GFP-signal is pseudo-colored to red, and Hoechst visualizes nuclei. The arrowhead points to punctate E-cadherin and arrows point to subcortical actin fibers. Scale bar, 10 μm (C) A representative example of a change from punctate to linear E-cadherin following α-actinin-1 downregulation (siA1) in MDA-MB-231 cells re-expressing E-cadherin. (D) Phalloidin (F-actin, green) and Hoechst (blue) co-stained HCC1937 cells following siRNA-mediated downregulation using non-targeting (siNT), α-actinin-1 (siA1) or α-actinin-4 (siA4) oligos, as indicated. Arrows show F-actin reorganization. Scale bar, 10 μm. (E) Zoom-in immunofluorescence images of E-cadherin or merged F-actin/E-cadherin following downregulation of control (siNT), α-actinin-1 (siA1) or α-actinin-4 (siA4), as indicated. (F) Immunofluorescence image of a wild-type HCC1937 cell stained for α-actinin-1 (purple) and merged image of α-actinin-1 (purple) and F-actin (green) to demonstrate the localization of endogenous α-actinin-1 on radial (white arrowheads) and arc-like (yellow arrows) actin fibers at E-cadherin based adhesion (see also Fig 5 Schematic Presentation). (G) Western blotting analysis with the indicated antibodies following downregulation of control (siNT), α-actinin-1 (siA1) or α-actinin-4 (siA4) in HCC1937 cells.
Curry Jonathan - One of the best experts on this subject based on the ideXlab platform.
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Low and heterogeneous prevalence of glucose-6-phosphate dehydrogenase deficiency in different settings in Ethiopia using phenotyping and genotyping approaches
Figshare, 2018Co-Authors: Shitaye Getasew, Gadisa Endalamaw, Grignard Lynn, Shumie Girma, Chali Wakweya, Menberu Temesgen, Belachew Mulualem, Tegegn Getaneh, Challi Sagni, Curry JonathanAbstract:8-Aminoquinolines such as primaquine clear mature Plasmodium falciparum gametocytes that are responsible for transmission from human to mosquitoes and bring radical cure in Plasmodium vivax by clearing dormant liver stages. Deployment of primaquine is thus of relevance for malaria elimination efforts but challenged by the widespread prevalence of glucose-6-phosphate dehydrogenase deficiency (G6PDd) in endemic countries since primaquine in G6PDd individuals may lead to acute haemolysis. In this study, the prevalence of G6PDd was investigated in different settings in Ethiopia using phenotyping and genotyping approaches. Collection consists of three files: File 1 is a flow chart for genotyping and data analysis. Indicated in a) is the Schematic Presentation of the quality check for data analysis and in b) is the genotyping procedure; File 2 contains KASP Primer sequences; and file 3 contains a Genotyping overview
Amir H. Noormohammadi - One of the best experts on this subject based on the ideXlab platform.
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Construction of pKS-VOTL plasmid.
2018Co-Authors: Muhammad A. Shahid, Marc S. Marenda, Philip F. Markham, Amir H. NoormohammadiAbstract:(A) Organisation of spo0B operon in B. subtilis and putative obg operon in M. synoviae has been shown. Putative –10 promoter region, transcription start site, ribosome binding site (RBS) of vlhA promoter region, and initiation codon for vlhA gene have been indicated. Length (bp) of each CDS is indicated inside the arrows. Identified stem loop structures in B. subtilis spo0B operon and putative stem loop in M. synoviae ‘obg operon’ have also been indicated. (B) Schematic Presentation of splicing by overlap extension (SOE) PCR to join vlhA gene promoter with obg CDS. Using PCR#1 and PCR#2, vlhA promoter (solid lines) and obg CDS (dotted lines) were amplified using indicated primers. Intermediate products with overlapping fragments (shown by horizontal bars) from both PCRs were amplified by SOE-PCR (PCR#3) using primers vlhA-ExtF and obg-ExtR. (C) Agarose gel electrophoresis of amplification products of vlhA promoter region PCR, obg CDS PCR and SOE-PCR. MW, DNA molecular weight marker (PCR Marker, Sigma, Missouri, USA). (D) Final product of SOE-PCR was first cloned at T-site of pGEM-T Easy Vector and then PstI-SacII fragment containing vlhA-obg was inserted between PstI and SacII sites of pBluescript II KS (+) vector. ApaI-SalI restriction fragment containing LoriC and tetM, from pMAS-LoriC plasmid, was cloned at respective sites in pBluescript II KS (+) vector, harboring vlhA-obg, to generate pKS-VOTL plasmid.
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Southern blot hybridisation to localise pKS-VOTL plasmid in MS-H transformants.
2018Co-Authors: Muhammad A. Shahid, Marc S. Marenda, Philip F. Markham, Amir H. NoormohammadiAbstract:(A) Schematic Presentation for the integration event of pKS-VOTL plasmid into genomic DNA of MS-H. A single putative homologous recombination event between the oriC copy carried by plasmid and chromosomal oriC region is represented by crossed lines. P indicates the promoter region of vlhA gene. (B, C, and D) BglII digested DNA of pKS-VOTL, untransformed MS-H and pKS-VOTL MS-H transformants MS-H-T75, MS-H-T78, MS-H-T90 (at passage 4th and 8th) was hybridised with DIG labeled tetM (B), oriC (C) and vlhA-obg probes (D).
