The Experts below are selected from a list of 213 Experts worldwide ranked by ideXlab platform
Shejiao Dai - One of the best experts on this subject based on the ideXlab platform.
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<B>SchizandrinB> B inhiBits the cis ddp induced apoptosis of hk 2 cells By activating erk nf κB signaling to regulate the expression of survivin
International Journal of Molecular Medicine, 2018Co-Authors: Qiang Liu, Jinxin Song, Lei Dong, Shejiao DaiAbstract:The nephrotoxicity of cisplatin limits its clinical application. <B>SchizandrinB> B (SchB) has Been demonstrated to have a variety of potential cytoprotective activities. The present study explored the molecular mechanisms By which SchB inhiBits the dichlorodiammine platinum (DDP)‑induced apoptosis of HK‑2 proximal tuBule epithelial cells. In vitro assays demonstrated that SchB increased the viaBility of HK‑2 cells, alleviated the cis‑DDP‑induced activation of caspase‑3, reduced apoptosis and improved the nuclear morphology of HK‑2 cells. Additionally, the mechanism underlying the cis‑DDP‑induced apoptosis was indicated to involve the activation of p53, c‑Jun‑N‑terminal kinase (JNK) and p38 signaling. Furthermore, SchB was demonstrated to activate extracellular signal‑regulated kinase (ERK) and nuclear factor κB (NF‑κB) signaling, and induce the expression of survivin. The inhiBition of ERK and NF‑κB signaling using U0126 and pyrollidine dithiocarBamate, respectively, inhiBited the expression of survivin, whereas Blocking the expression of survivin using small interfering RNA inhiBited the alleviating effect of SchB on cis‑DDP‑induced apoptosis as indicated By a reduction in cleaved caspase‑3 expression. In conclusion, SchB regulates ERK/NF‑κB signaling to induce the expression of survivin, thereBy alleviating cis‑DDP‑induced renal injury.
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<B>SchizandrinB> B inhiBits the cis‑DDP‑induced apoptosis of HK‑2 cells By activating ERK/NF‑κB signaling to regulate the expression of survivin.
International journal of molecular medicine, 2018Co-Authors: Qiang Liu, Jinxin Song, Lei Dong, Shejiao DaiAbstract:The nephrotoxicity of cisplatin limits its clinical application. <B>SchizandrinB> B (SchB) has Been demonstrated to have a variety of potential cytoprotective activities. The present study explored the molecular mechanisms By which SchB inhiBits the dichlorodiammine platinum (DDP)‑induced apoptosis of HK‑2 proximal tuBule epithelial cells. In vitro assays demonstrated that SchB increased the viaBility of HK‑2 cells, alleviated the cis‑DDP‑induced activation of caspase‑3, reduced apoptosis and improved the nuclear morphology of HK‑2 cells. Additionally, the mechanism underlying the cis‑DDP‑induced apoptosis was indicated to involve the activation of p53, c‑Jun‑N‑terminal kinase (JNK) and p38 signaling. Furthermore, SchB was demonstrated to activate extracellular signal‑regulated kinase (ERK) and nuclear factor κB (NF‑κB) signaling, and induce the expression of survivin. The inhiBition of ERK and NF‑κB signaling using U0126 and pyrollidine dithiocarBamate, respectively, inhiBited the expression of survivin, whereas Blocking the expression of survivin using small interfering RNA inhiBited the alleviating effect of SchB on cis‑DDP‑induced apoptosis as indicated By a reduction in cleaved caspase‑3 expression. In conclusion, SchB regulates ERK/NF‑κB signaling to induce the expression of survivin, thereBy alleviating cis‑DDP‑induced renal injury.
Shuyu Zhan - One of the best experts on this subject based on the ideXlab platform.
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Development of a sensitive LC‐MS/MS method for simultaneous quantification of eleven constituents in rat serum and its application to a pharmacokinetic study of a Chinese medicine Shengmai injection
Biomedical chromatography : BMC, 2014Co-Authors: Shuyu Zhan, Qing Shao, Xiaohui FanAbstract:A sensitive LC-MS/MS method was developed and validated for simultaneous quantification of 11 constituents, ginsenoside Rg1, Re, Rf, Rg2, RB1, Rd, Rc, ophiopogonin D, schisandrin, schisandrol B and <B>SchizandrinB> B, in rat serum using digoxin as the internal standard (IS). The serum samples were pretreated and extracted with a two-step liquid-liquid extraction. Chromatographic separation was achieved on a C18 analytical column with a proper gradient elution using 0.02% acetic acid aqueous solution and 0.02% acetic acid-acetonitrile as moBile phase at a flow rate of 0.5 mL/min. MS detection was performed using multiple reaction monitoring via an electrospray ionization source. Good linearity was oBserved in the validated concentration range for every analyte (r(2) ≥0.9929), and the lower limits of quantitation of the analytes were in the range of 0.044-1.190 ng/mL in rat serum. Intra- and inter-day precisions were 75.8%.The validated method was successfully applied to a pharmacokinetic study of all analytes in rats after single intravenous administration of Shengmai injection.
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Tissue distriBution and excretion of herBal components after intravenous administration of a Chinese medicine (Shengmai injection) in rat
Archives of pharmacal research, 2014Co-Authors: Shuyu Zhan, Qing Shao, Xiaohui Fan, Yiyu ChengAbstract:Shengmai injection, consisting of Panax ginseng, Radix ophiopogonis and Schisandra chinensis, is a widely used Chinese medicine for the treatment of various cardiovascular diseases. In this study, tissue distriBution and excretion of its multiple active components including protopanaxatriol-type (Ppt-type) ginsenosides (ginsenoside Rg1, Re, Rf and Rg2), protopanaxadiol-type (Ppd-type) ginsenosides (ginsenoside RB1, Rd and Rc), ophiopogonin (ophiopogonin D), and lignan (schisandrin, schisandrol B and <B>SchizandrinB> B) in rat after single intravenous administration of Shengmai injection were reported. Ppt-type ginsenosides exhiBited quick and wide distriBution from Blood into tissues and were eliminated rapidly through Biliary, urinary and fecal excretions. Ppd-type ginsenosides RB1, Rd and Rc distriButed quickly from Blood to all tissues But exhiBited slow elimination By Biliary and urinary excretions. Ophiopogonin D was excreted into Bile with no urinary and fecal excretion, indicating its elimination in the form of secondary metaBolites. Schisandrin, schisandrol B and <B>SchizandrinB> B was found to distriBute quickly from Blood into most tissues and had accumulation in these tissues. Very low Biliary, urinary and fecal excretion implied that lignan was mainly excreted in the form of their metaBolites. This study produced a first hand in vivo tissue distriBution and dynamic profiles of the active components of Shengmai injection, providing valuaBle information for drug development and clinical application of Shengmai injection.
Yiyu Cheng - One of the best experts on this subject based on the ideXlab platform.
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Tissue distriBution and excretion of herBal components after intravenous administration of a Chinese medicine (Shengmai injection) in rat
Archives of pharmacal research, 2014Co-Authors: Shuyu Zhan, Qing Shao, Xiaohui Fan, Yiyu ChengAbstract:Shengmai injection, consisting of Panax ginseng, Radix ophiopogonis and Schisandra chinensis, is a widely used Chinese medicine for the treatment of various cardiovascular diseases. In this study, tissue distriBution and excretion of its multiple active components including protopanaxatriol-type (Ppt-type) ginsenosides (ginsenoside Rg1, Re, Rf and Rg2), protopanaxadiol-type (Ppd-type) ginsenosides (ginsenoside RB1, Rd and Rc), ophiopogonin (ophiopogonin D), and lignan (schisandrin, schisandrol B and <B>SchizandrinB> B) in rat after single intravenous administration of Shengmai injection were reported. Ppt-type ginsenosides exhiBited quick and wide distriBution from Blood into tissues and were eliminated rapidly through Biliary, urinary and fecal excretions. Ppd-type ginsenosides RB1, Rd and Rc distriButed quickly from Blood to all tissues But exhiBited slow elimination By Biliary and urinary excretions. Ophiopogonin D was excreted into Bile with no urinary and fecal excretion, indicating its elimination in the form of secondary metaBolites. Schisandrin, schisandrol B and <B>SchizandrinB> B was found to distriBute quickly from Blood into most tissues and had accumulation in these tissues. Very low Biliary, urinary and fecal excretion implied that lignan was mainly excreted in the form of their metaBolites. This study produced a first hand in vivo tissue distriBution and dynamic profiles of the active components of Shengmai injection, providing valuaBle information for drug development and clinical application of Shengmai injection.
Qiang Liu - One of the best experts on this subject based on the ideXlab platform.
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<B>SchizandrinB> B inhiBits the cis ddp induced apoptosis of hk 2 cells By activating erk nf κB signaling to regulate the expression of survivin
International Journal of Molecular Medicine, 2018Co-Authors: Qiang Liu, Jinxin Song, Lei Dong, Shejiao DaiAbstract:The nephrotoxicity of cisplatin limits its clinical application. <B>SchizandrinB> B (SchB) has Been demonstrated to have a variety of potential cytoprotective activities. The present study explored the molecular mechanisms By which SchB inhiBits the dichlorodiammine platinum (DDP)‑induced apoptosis of HK‑2 proximal tuBule epithelial cells. In vitro assays demonstrated that SchB increased the viaBility of HK‑2 cells, alleviated the cis‑DDP‑induced activation of caspase‑3, reduced apoptosis and improved the nuclear morphology of HK‑2 cells. Additionally, the mechanism underlying the cis‑DDP‑induced apoptosis was indicated to involve the activation of p53, c‑Jun‑N‑terminal kinase (JNK) and p38 signaling. Furthermore, SchB was demonstrated to activate extracellular signal‑regulated kinase (ERK) and nuclear factor κB (NF‑κB) signaling, and induce the expression of survivin. The inhiBition of ERK and NF‑κB signaling using U0126 and pyrollidine dithiocarBamate, respectively, inhiBited the expression of survivin, whereas Blocking the expression of survivin using small interfering RNA inhiBited the alleviating effect of SchB on cis‑DDP‑induced apoptosis as indicated By a reduction in cleaved caspase‑3 expression. In conclusion, SchB regulates ERK/NF‑κB signaling to induce the expression of survivin, thereBy alleviating cis‑DDP‑induced renal injury.
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<B>SchizandrinB> B inhiBits the cis‑DDP‑induced apoptosis of HK‑2 cells By activating ERK/NF‑κB signaling to regulate the expression of survivin.
International journal of molecular medicine, 2018Co-Authors: Qiang Liu, Jinxin Song, Lei Dong, Shejiao DaiAbstract:The nephrotoxicity of cisplatin limits its clinical application. <B>SchizandrinB> B (SchB) has Been demonstrated to have a variety of potential cytoprotective activities. The present study explored the molecular mechanisms By which SchB inhiBits the dichlorodiammine platinum (DDP)‑induced apoptosis of HK‑2 proximal tuBule epithelial cells. In vitro assays demonstrated that SchB increased the viaBility of HK‑2 cells, alleviated the cis‑DDP‑induced activation of caspase‑3, reduced apoptosis and improved the nuclear morphology of HK‑2 cells. Additionally, the mechanism underlying the cis‑DDP‑induced apoptosis was indicated to involve the activation of p53, c‑Jun‑N‑terminal kinase (JNK) and p38 signaling. Furthermore, SchB was demonstrated to activate extracellular signal‑regulated kinase (ERK) and nuclear factor κB (NF‑κB) signaling, and induce the expression of survivin. The inhiBition of ERK and NF‑κB signaling using U0126 and pyrollidine dithiocarBamate, respectively, inhiBited the expression of survivin, whereas Blocking the expression of survivin using small interfering RNA inhiBited the alleviating effect of SchB on cis‑DDP‑induced apoptosis as indicated By a reduction in cleaved caspase‑3 expression. In conclusion, SchB regulates ERK/NF‑κB signaling to induce the expression of survivin, thereBy alleviating cis‑DDP‑induced renal injury.
Xiaohui Fan - One of the best experts on this subject based on the ideXlab platform.
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Development of a sensitive LC‐MS/MS method for simultaneous quantification of eleven constituents in rat serum and its application to a pharmacokinetic study of a Chinese medicine Shengmai injection
Biomedical chromatography : BMC, 2014Co-Authors: Shuyu Zhan, Qing Shao, Xiaohui FanAbstract:A sensitive LC-MS/MS method was developed and validated for simultaneous quantification of 11 constituents, ginsenoside Rg1, Re, Rf, Rg2, RB1, Rd, Rc, ophiopogonin D, schisandrin, schisandrol B and <B>SchizandrinB> B, in rat serum using digoxin as the internal standard (IS). The serum samples were pretreated and extracted with a two-step liquid-liquid extraction. Chromatographic separation was achieved on a C18 analytical column with a proper gradient elution using 0.02% acetic acid aqueous solution and 0.02% acetic acid-acetonitrile as moBile phase at a flow rate of 0.5 mL/min. MS detection was performed using multiple reaction monitoring via an electrospray ionization source. Good linearity was oBserved in the validated concentration range for every analyte (r(2) ≥0.9929), and the lower limits of quantitation of the analytes were in the range of 0.044-1.190 ng/mL in rat serum. Intra- and inter-day precisions were 75.8%.The validated method was successfully applied to a pharmacokinetic study of all analytes in rats after single intravenous administration of Shengmai injection.
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Tissue distriBution and excretion of herBal components after intravenous administration of a Chinese medicine (Shengmai injection) in rat
Archives of pharmacal research, 2014Co-Authors: Shuyu Zhan, Qing Shao, Xiaohui Fan, Yiyu ChengAbstract:Shengmai injection, consisting of Panax ginseng, Radix ophiopogonis and Schisandra chinensis, is a widely used Chinese medicine for the treatment of various cardiovascular diseases. In this study, tissue distriBution and excretion of its multiple active components including protopanaxatriol-type (Ppt-type) ginsenosides (ginsenoside Rg1, Re, Rf and Rg2), protopanaxadiol-type (Ppd-type) ginsenosides (ginsenoside RB1, Rd and Rc), ophiopogonin (ophiopogonin D), and lignan (schisandrin, schisandrol B and <B>SchizandrinB> B) in rat after single intravenous administration of Shengmai injection were reported. Ppt-type ginsenosides exhiBited quick and wide distriBution from Blood into tissues and were eliminated rapidly through Biliary, urinary and fecal excretions. Ppd-type ginsenosides RB1, Rd and Rc distriButed quickly from Blood to all tissues But exhiBited slow elimination By Biliary and urinary excretions. Ophiopogonin D was excreted into Bile with no urinary and fecal excretion, indicating its elimination in the form of secondary metaBolites. Schisandrin, schisandrol B and <B>SchizandrinB> B was found to distriBute quickly from Blood into most tissues and had accumulation in these tissues. Very low Biliary, urinary and fecal excretion implied that lignan was mainly excreted in the form of their metaBolites. This study produced a first hand in vivo tissue distriBution and dynamic profiles of the active components of Shengmai injection, providing valuaBle information for drug development and clinical application of Shengmai injection.
