The Experts below are selected from a list of 129 Experts worldwide ranked by ideXlab platform
Rebecca R Stanway - One of the best experts on this subject based on the ideXlab platform.
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plasmodium condensin core subunits smc2 smc4 mediate atypical mitosis and are essential for parasite proliferation and transmission
Cell Reports, 2020Co-Authors: Rajan Pandey, Steven Abel, Matthew Boucher, Richard J Wall, Mohammad Zeeshan, Aline Freville, Xueqing Maggie Lu, Declan Brady, Emilie Daniel, Rebecca R StanwayAbstract:Summary Condensin is a multi-subunit protein complex regulating chromosome condensation and segregation during cell division. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin, during endomitosis in Schizogony and endoreduplication in male gametogenesis. During early Schizogony, SMC2/SMC4 localize to a distinct focus, identified as the centromeres by NDC80 fluorescence and chromatin immunoprecipitation sequencing (ChIP-seq) analyses, but do not form condensin I or II complexes. In mature schizonts and during male gametogenesis, there is a diffuse SMC2/SMC4 distribution on chromosomes and in the nucleus, and both condensin I and condensin II complexes form at these stages. Knockdown of smc2 and smc4 gene expression reveals essential roles in parasite proliferation and transmission. The condensin core subunits (SMC2/SMC4) form different complexes and may have distinct functions at various stages of the parasite life cycle.
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plasmodium condensin core subunits smc2 smc4 mediate atypical mitosis and are essential for parasite proliferation and transmission
bioRxiv, 2019Co-Authors: Rajan Pandey, Steven Abel, Matthew Boucher, Richard J Wall, Mohammad Zeeshan, Aline Freville, Xueqing Maggie Lu, Declan Brady, Emilie Daniel, Rebecca R StanwayAbstract:Condensin is a multi-subunit protein complex that regulates chromosome organization, segregation and condensation during cell division in eukaryotes. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin during endomitosis in Schizogony and endoreduplication in male gametogenesis. SMC2 and SMC4 localize at discrete foci during Schizogony, and with a diffuse nuclear distribution during male gametogenesis. ChIP-seq analyses suggest a centromeric location of SMC2/SMC4 only during Schizogony. Co-immunoprecipitation data reveal the presence of both condensin complex I and II during male gametogenesis, but only the SMC2/SMC4 heterodimer during Schizogony. Finally, knockdown of smc2 and smc4 gene expression revealed their essential roles in parasite proliferation and transmission. This study shows that condensin core subunits (SMC2/SMC4) have differential complex and distinct functions at different stages of the parasite life cycle.
K H Horton - One of the best experts on this subject based on the ideXlab platform.
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sarcocystis neurona and sarcocystis falcatula monitoring of Schizogony in cell culture using fluorescent nuclear labeling
Veterinary Parasitology, 2001Co-Authors: Dwight D Bowman, M A Iordanescu, H H Chou, K H HortonAbstract:Abstract The nuclei of merozoites of Sarcocystis neurona and Sarcocystis falcatula were labeled with the fluorescent marker Syto®21. It was shown that the marker would label the parasites and that they would retain the marker throughout Schizogony. Thus, there was sufficient marker in the daughter merozoites to make them easily visible with fluorescence microscopy. This technique will be helpful in studying the developmental biology of these parasites in vitro.
Rajan Pandey - One of the best experts on this subject based on the ideXlab platform.
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plasmodium condensin core subunits smc2 smc4 mediate atypical mitosis and are essential for parasite proliferation and transmission
Cell Reports, 2020Co-Authors: Rajan Pandey, Steven Abel, Matthew Boucher, Richard J Wall, Mohammad Zeeshan, Aline Freville, Xueqing Maggie Lu, Declan Brady, Emilie Daniel, Rebecca R StanwayAbstract:Summary Condensin is a multi-subunit protein complex regulating chromosome condensation and segregation during cell division. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin, during endomitosis in Schizogony and endoreduplication in male gametogenesis. During early Schizogony, SMC2/SMC4 localize to a distinct focus, identified as the centromeres by NDC80 fluorescence and chromatin immunoprecipitation sequencing (ChIP-seq) analyses, but do not form condensin I or II complexes. In mature schizonts and during male gametogenesis, there is a diffuse SMC2/SMC4 distribution on chromosomes and in the nucleus, and both condensin I and condensin II complexes form at these stages. Knockdown of smc2 and smc4 gene expression reveals essential roles in parasite proliferation and transmission. The condensin core subunits (SMC2/SMC4) form different complexes and may have distinct functions at various stages of the parasite life cycle.
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plasmodium condensin core subunits smc2 smc4 mediate atypical mitosis and are essential for parasite proliferation and transmission
bioRxiv, 2019Co-Authors: Rajan Pandey, Steven Abel, Matthew Boucher, Richard J Wall, Mohammad Zeeshan, Aline Freville, Xueqing Maggie Lu, Declan Brady, Emilie Daniel, Rebecca R StanwayAbstract:Condensin is a multi-subunit protein complex that regulates chromosome organization, segregation and condensation during cell division in eukaryotes. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin during endomitosis in Schizogony and endoreduplication in male gametogenesis. SMC2 and SMC4 localize at discrete foci during Schizogony, and with a diffuse nuclear distribution during male gametogenesis. ChIP-seq analyses suggest a centromeric location of SMC2/SMC4 only during Schizogony. Co-immunoprecipitation data reveal the presence of both condensin complex I and II during male gametogenesis, but only the SMC2/SMC4 heterodimer during Schizogony. Finally, knockdown of smc2 and smc4 gene expression revealed their essential roles in parasite proliferation and transmission. This study shows that condensin core subunits (SMC2/SMC4) have differential complex and distinct functions at different stages of the parasite life cycle.
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Plasmodium condensin core subunits (SMC2/SMC4) mediate atypical mitosis and are essential for parasite proliferation and transmission
bioRxiv, 2019Co-Authors: Rajan Pandey, Steven Abel, Matthew Boucher, Richard J Wall, Mohammad Zeeshan, Aline Freville, Xueqing Maggie Lu, Declan Brady, Emilie DanielAbstract:Condensin is a multi-subunit protein complex that regulates chromosome organization, segregation and condensation during cell division in eukaryotes. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin during endomitosis in Schizogony and endoreduplication in male gametogenesis. SMC2 and SMC4 localize at discrete foci during Schizogony, and with a diffuse nuclear distribution during male gametogenesis. ChIP-seq analyses suggest a centromeric location of SMC2/SMC4 only during Schizogony. Co-immunoprecipitation data reveal the presence of both condensin complex I and II during male gametogenesis, but only the SMC2/SMC4 heterodimer during Schizogony. Finally, knockdown of smc2 and smc4 gene expression revealed their essential roles in parasite proliferation and transmission. This study shows that condensin core subunits (SMC2/SMC4) have differential complex and distinct functions at different stages of the parasite life cycle.
J P Dubey - One of the best experts on this subject based on the ideXlab platform.
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Schizogony and gametogony of oocyst deficient t 263 strain of toxoplasma gondii
Veterinary Parasitology, 2017Co-Authors: J P DubeyAbstract:Abstract Oocysts are important stage for the spread of Toxoplasma gondii because they are environmentally resistant. Among all hosts of T. gondii, only felids can excrete oocysts. Cats that have excreted T. gondii oocysts after primary infection develop immunity to re-excretion of oocysts, and this immunity appears to be long-lasting. It would be desirable to have a non-infectious vaccine for the prevention of T. gondii infection in cats and to understand mechanism of immunity to excretion of oocysts. An initial step will be to indentify stage/stages of the parasite for induction of immunity. A chemically-induced mutant of T. gondii, T-263, is immunogenic but lacks the capacity to form oocysts in cats. Cats fed live bradyzoites of T-263 do not excrete oocysts after challenge with oocyst producing strains. However, it is not known at what stage of the parasite development the oocyst formation is halted. Here, four cats were fed live tissue cysts of the T-263 strain and examined for enteroepithelial stages and oocyst production. Two cats were administered methyl prednisolone aceatate (20 mg/kg) once intramuscularly and these cats were euthanized 5 and 7 days post inoculation. No oocysts but immature and mature schizonts (types D and E), male, and female gamonts were detected in two cats euthanized. The remaining two cats did not excrete oocysts examined 3–14 days post inoculation, but both seroconverted and developed antibody titers of 1:400 tested by the modification agglutination test, indicating exposure to the inocula. The results demonstrate that the T-263 strain is defective in oocyst formation and the observations should help future studies in identification of genes/factors responsible for oocyst formation in the intestine of cats.
Emilie Daniel - One of the best experts on this subject based on the ideXlab platform.
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plasmodium condensin core subunits smc2 smc4 mediate atypical mitosis and are essential for parasite proliferation and transmission
Cell Reports, 2020Co-Authors: Rajan Pandey, Steven Abel, Matthew Boucher, Richard J Wall, Mohammad Zeeshan, Aline Freville, Xueqing Maggie Lu, Declan Brady, Emilie Daniel, Rebecca R StanwayAbstract:Summary Condensin is a multi-subunit protein complex regulating chromosome condensation and segregation during cell division. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin, during endomitosis in Schizogony and endoreduplication in male gametogenesis. During early Schizogony, SMC2/SMC4 localize to a distinct focus, identified as the centromeres by NDC80 fluorescence and chromatin immunoprecipitation sequencing (ChIP-seq) analyses, but do not form condensin I or II complexes. In mature schizonts and during male gametogenesis, there is a diffuse SMC2/SMC4 distribution on chromosomes and in the nucleus, and both condensin I and condensin II complexes form at these stages. Knockdown of smc2 and smc4 gene expression reveals essential roles in parasite proliferation and transmission. The condensin core subunits (SMC2/SMC4) form different complexes and may have distinct functions at various stages of the parasite life cycle.
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plasmodium condensin core subunits smc2 smc4 mediate atypical mitosis and are essential for parasite proliferation and transmission
bioRxiv, 2019Co-Authors: Rajan Pandey, Steven Abel, Matthew Boucher, Richard J Wall, Mohammad Zeeshan, Aline Freville, Xueqing Maggie Lu, Declan Brady, Emilie Daniel, Rebecca R StanwayAbstract:Condensin is a multi-subunit protein complex that regulates chromosome organization, segregation and condensation during cell division in eukaryotes. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin during endomitosis in Schizogony and endoreduplication in male gametogenesis. SMC2 and SMC4 localize at discrete foci during Schizogony, and with a diffuse nuclear distribution during male gametogenesis. ChIP-seq analyses suggest a centromeric location of SMC2/SMC4 only during Schizogony. Co-immunoprecipitation data reveal the presence of both condensin complex I and II during male gametogenesis, but only the SMC2/SMC4 heterodimer during Schizogony. Finally, knockdown of smc2 and smc4 gene expression revealed their essential roles in parasite proliferation and transmission. This study shows that condensin core subunits (SMC2/SMC4) have differential complex and distinct functions at different stages of the parasite life cycle.
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Plasmodium condensin core subunits (SMC2/SMC4) mediate atypical mitosis and are essential for parasite proliferation and transmission
bioRxiv, 2019Co-Authors: Rajan Pandey, Steven Abel, Matthew Boucher, Richard J Wall, Mohammad Zeeshan, Aline Freville, Xueqing Maggie Lu, Declan Brady, Emilie DanielAbstract:Condensin is a multi-subunit protein complex that regulates chromosome organization, segregation and condensation during cell division in eukaryotes. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin during endomitosis in Schizogony and endoreduplication in male gametogenesis. SMC2 and SMC4 localize at discrete foci during Schizogony, and with a diffuse nuclear distribution during male gametogenesis. ChIP-seq analyses suggest a centromeric location of SMC2/SMC4 only during Schizogony. Co-immunoprecipitation data reveal the presence of both condensin complex I and II during male gametogenesis, but only the SMC2/SMC4 heterodimer during Schizogony. Finally, knockdown of smc2 and smc4 gene expression revealed their essential roles in parasite proliferation and transmission. This study shows that condensin core subunits (SMC2/SMC4) have differential complex and distinct functions at different stages of the parasite life cycle.
