The Experts below are selected from a list of 150 Experts worldwide ranked by ideXlab platform
Shoichi Nakagawa - One of the best experts on this subject based on the ideXlab platform.
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3 cases of anti Scl 70 topoisomerase i Antibody associated with central nervous system lupus without renal disorder
The Journal of Rheumatology, 1993Co-Authors: Masaya Mukai, Akira Sagawa, T Atsumi, S Jodo, Yoshiharu Amasaki, Toru Nakabayashi, Ichiro Watanabe, Atsushi Fujisaku, Shoichi NakagawaAbstract:We describe 3 cases of systemic lupus erythematosus (SLE) associated with anti-Scl-70 Antibody. Common symptoms were central nervous system disorder, discoid rash, lymphadenopathy, and no renal disorder. Two of 3 cases showed some symptoms of Scleroderma but could not be diagnosed as such. Although further followup is required to determine if Scleroderma develops, these unique symptoms might be a subtype of SLE or a lupus-like syndrome characterized by symptoms and anti-Scl-70 Antibody.
Masaya Mukai - One of the best experts on this subject based on the ideXlab platform.
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3 cases of anti Scl 70 topoisomerase i Antibody associated with central nervous system lupus without renal disorder
The Journal of Rheumatology, 1993Co-Authors: Masaya Mukai, Akira Sagawa, T Atsumi, S Jodo, Yoshiharu Amasaki, Toru Nakabayashi, Ichiro Watanabe, Atsushi Fujisaku, Shoichi NakagawaAbstract:We describe 3 cases of systemic lupus erythematosus (SLE) associated with anti-Scl-70 Antibody. Common symptoms were central nervous system disorder, discoid rash, lymphadenopathy, and no renal disorder. Two of 3 cases showed some symptoms of Scleroderma but could not be diagnosed as such. Although further followup is required to determine if Scleroderma develops, these unique symptoms might be a subtype of SLE or a lupus-like syndrome characterized by symptoms and anti-Scl-70 Antibody.
F L Tang - One of the best experts on this subject based on the ideXlab platform.
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detection of anti Scl 70 Antibody by recombinant fusion peptide enzyme linked immunosorbent assay a preliminary study
Chinese Journal of Internal Medicine, 2006Co-Authors: Z Chen, G S Wang, F L TangAbstract:Objective To detect anti-Scl-70 Antibody with enzyme-linked immunosorbent assay(ELISA)using a recombinant fusion peptide which comprises 207-765 amino acid fragment of Scl-70 as antigen.Methods cDNA encoding Scl-70 antigen fragment from aa207 to aa765 from human placenta cDNA first strand was amplified with PCR.The obtained cDNA was inserted into expression vector Pet-42b and transferred into E.coli BL21(DE3)for expression of his-tagged fusion peptide.After Ni 2+-NTA affinity purification,the antigenicity was identified with Western blotting.Serum samples from 36 systemic Sclerosis(SSc)patients,20 patients with other connective tissue disorder(CTD)and 30 normal controls were retrospectively tested with ELISA.Results A soluble fusion peptide was expressed and purified.The antigenicity was confirmed with Western blotting using standard positive anti-Scl-70 Antibody serum.Of the 36 serum samples from SSc patients,5 of 6 samples showing positive reaction with natural Scl-70 antigen in double immunodifussion(DID)assay also recognized the recombinant fusion peptide with ELISA,only one serum sample which showed positive anti-Scl-70 in DID displayed a negative result in ELISA assay.On the contrary,3 patients with negative anti-Scl-70 in DID showed positive results in ELISA assay using this recombinant peptide.All the serum samples from patients with other CTD showed negative results in ELISA assay.Conclusion The recombinant antigen fragment contains major epitope regions in natural Scl-70 antigen.Detection of anti-Scl-70 Antibody with ELISA using the recombinant peptide can improve the sensitivity and has a potential role in determining its clinical association.
Toru Nakabayashi - One of the best experts on this subject based on the ideXlab platform.
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3 cases of anti Scl 70 topoisomerase i Antibody associated with central nervous system lupus without renal disorder
The Journal of Rheumatology, 1993Co-Authors: Masaya Mukai, Akira Sagawa, T Atsumi, S Jodo, Yoshiharu Amasaki, Toru Nakabayashi, Ichiro Watanabe, Atsushi Fujisaku, Shoichi NakagawaAbstract:We describe 3 cases of systemic lupus erythematosus (SLE) associated with anti-Scl-70 Antibody. Common symptoms were central nervous system disorder, discoid rash, lymphadenopathy, and no renal disorder. Two of 3 cases showed some symptoms of Scleroderma but could not be diagnosed as such. Although further followup is required to determine if Scleroderma develops, these unique symptoms might be a subtype of SLE or a lupus-like syndrome characterized by symptoms and anti-Scl-70 Antibody.
Akira Sagawa - One of the best experts on this subject based on the ideXlab platform.
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3 cases of anti Scl 70 topoisomerase i Antibody associated with central nervous system lupus without renal disorder
The Journal of Rheumatology, 1993Co-Authors: Masaya Mukai, Akira Sagawa, T Atsumi, S Jodo, Yoshiharu Amasaki, Toru Nakabayashi, Ichiro Watanabe, Atsushi Fujisaku, Shoichi NakagawaAbstract:We describe 3 cases of systemic lupus erythematosus (SLE) associated with anti-Scl-70 Antibody. Common symptoms were central nervous system disorder, discoid rash, lymphadenopathy, and no renal disorder. Two of 3 cases showed some symptoms of Scleroderma but could not be diagnosed as such. Although further followup is required to determine if Scleroderma develops, these unique symptoms might be a subtype of SLE or a lupus-like syndrome characterized by symptoms and anti-Scl-70 Antibody.