The Experts below are selected from a list of 5616 Experts worldwide ranked by ideXlab platform
Ma Jia-hao - One of the best experts on this subject based on the ideXlab platform.
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Pathology of scuticociliatosis in Scophthalmus Maximus L.
Journal of fishery sciences of China, 2008Co-Authors: Ma Jia-haoAbstract:Scuticociliate is a kind of organisms harmful to the marine breeding and scuticociliatosis is suggested to be one of the most important diseases in industrial fish-breeding. Pathological study on the disease will be helpful to understand the mechanism of scuticociliatosis of turbot (Scophthalmus Maximus L). Pathological changes on scuticociliatosis of the turbot were studied with histological methods. Scuticociliates were found in connective tissue and ulcerative parts in skin and fin,tissue around anus,tissue around muscle fibers,gill filament,brain,spinal cord,wall and cavity of eyeball,liver,tissue around gland duct of pancreas,kidney,adventitia of spleen,wall of stomach,blood vessel in wall of intestine,wall of bladder,gonad and tissue around gonad,and connective tissue around all organs of viscus. They were also found in body fluid,such as blood, cerebrospinal fluid,ascites,gastric juice and intestinal juice. Obvious pathological changes occurred in positions where a large number of ciliates invaded,especially in skin,fin,tissue around anus, gill,brain,spinal cord,eyeball,liver and wall of stomach. The invaded fish showed congestion and hemorrhage,necrosis and breaking up of cells,and hyperplasia of lymphocytes. The pathological changes,however,were not visible in tissues when a small quantity of ciliates invaded. The study suggests that ciliates have no obvious tissue selectivity to the turbot.
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Histological and histochemical study on the digestive system of Scophthalmus Maximus
Marine Fisheries Research, 2008Co-Authors: Ma Jia-haoAbstract:To investigate the digestive mechanism of Scophthalmus Maximus L., the digestive system was studied with histological and histochemical methods. The epithelium of the stomach consisted of columnar cells. The epithelium of the gastric gland was made up of type Ⅰ and type Ⅱ cells. The epithelium of the pyloric caecum and the intestine consisted of columnar cells and different numbers of mucous cells. The columnar cells of the stomach showed activity of acid phosphatase and weak lipase. The type Ⅰcells of the gastric gland secreted mucous, and the type Ⅱ cells showed activity of proteinase. The columnar cells of the pyloric caecum and the intestine showed activity of proteinase, lipase, non-specific esterase, and the cytoplast and plasma membrane of their free ends showed activity of acid phosphatase and alkaline phosphatase. The hepatocytes showed activity of proteinase, and the function of storing glycogen and fat. The pancrease only showed weak activity of lipase and non-specific esterase.
Songlin Chen - One of the best experts on this subject based on the ideXlab platform.
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draft genomes of female and male turbot Scophthalmus Maximus
Scientific Data, 2020Co-Authors: Changwei Shao, Qian Zhou, Feng You, Na Wang, Songlin ChenAbstract:Turbot (Scophthalmus Maximus) is a commercially important flatfish species in aquaculture. It has a drastic sexual dimorphism, with females growing faster than males. In the present study, we sequenced and de novo assembled female and male turbot genomes. The assembled female genome was 568 Mb (scaffold N50, 6.2 Mb, BUSCO 97.4%), and the male genome was 584 Mb (scaffold N50, 5.9 Mb, BUSCO 96.6%). Using two genetic maps, we anchored female scaffolds representing 535 Mb onto 22 chromosomes. Annotation of the female anchored genome identified 87.8 Mb transposon elements and 20,134 genes. We identified 17,936 gene families, of which 369 gene families were flatfish specific. Phylogenetic analysis showed that the turbot, Japanese flounder and Chinese tongue sole form a clade that diverged from other teleosts approximately 78 Mya. This report of female and male turbot draft genomes and annotated genes provides a new resource for identifying sex determination genes, elucidating the evolution of adaptive traits in flatfish and developing genetic techniques to increase the sustainability of turbot aquaculture. Machine-accessible metadata file describing the reported data: https://doi.org/10.6084/m9.figshare.11891598
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isolation and characterization of polymorphic microsatellite loci from an est library of turbot Scophthalmus Maximus and cross species amplification
Molecular Ecology Notes, 2007Co-Authors: Songlin Chen, Y Jiang, Xiaolin Liao, L MengAbstract:In the present study, we report 12 polymorphic microsatellite loci developed from a cDNA library from the turbot, Scophthalmus Maximus. Observed and expected heterozygosities varied from 0.20 to 1.00 and from 0.18 to 0.78, respectively. No significant linkage disequilibrium between pairs of loci was found, but two loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. Cross-species amplifications of these microsatellites in five additional fish species revealed between five and 11 positive amplifications and between zero and four polymorphic loci per species.
S. H. Coombs - One of the best experts on this subject based on the ideXlab platform.
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Histological changes in starved turbot larvae (Scophthalmus Maximus) quantified by digital image analysis
Journal of Fish Biology, 1994Co-Authors: Irb Mcfadzen, D. M. Lowe, S. H. CoombsAbstract:In turbot larvae, Scophthalmus Maximus, deprived of food for 24 h there was a significant increase in the specific surface of the epithelium and the corresponding microvillous border of the foregut accompanied by slight cellular degeneration. Following 48 h starvation Sarvae showed severe tissue degeneration in the foregut mucosa, progressing to extensive mucosal desquamation and cellular sloughing. Intracellular vacuolation of the epithelium and loss of microvilli was also extensive and the ability of the gut to absorb food must be severely impaired, with further starvation probably resulting in larval death. There was no evidence of parasitic infection in any of the larvae sampled and all observed alterations are attributable to food deprivation.
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Digestion of natural food by larval and post-larval turbot Scophthalmus Maximus
Marine Ecology Progress Series, 1993Co-Authors: D. V. P. Conway, Prg Tranter, S. H. CoombsAbstract:The digestion of natural, mainly crustacean zooplankton, by different age groups of turbot Scophthalmus Maximus larvae was evaluated by comparisons of visual appearance, dry weight and carbon and nitrogen content of fresh food organisms with material recovered from faeces. Visually, the degree of digestion of food particles ranged from no discernible change of lamellibranch larvae, copepod eggs, intact copepod faecal pellets and some phytoplankton species, to varying degrees of removal of body constituents in copepods, cladocerans and decapod zoea. For crustaceans, the proportion of body constituents removed was related to the size and construction of their apparently indigestible exoskeleton. Uppon defaecation larger organisms showed the greatest percentage loss in dry weight and carbon. A high percentage of nitrogen was extracted from all organisms.
Albert K. Imsland - One of the best experts on this subject based on the ideXlab platform.
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The Shelf Life of Farmed Turbot (Scophthalmus Maximus)
Journal of food science, 2014Co-Authors: Bjorn Roth, Lene Kramer, Aase Vorre Skuland, Trond Løvdal, Sigurd Øines, Atle Foss, Albert K. ImslandAbstract:A total of 18 farmed turbot (Scophthalmus Maximus) were slaughtered over 4 successive weeks in November 2012 and stored in polystyrene boxes with ice until analyzed. The fish were stored between 1 and 22 d and presented to a taste panel and further analyzed for quality index method (QIM), microbiological analysis by real-time quantitative PCR (qPCR), taste, pH, color by computer imaging, protein denaturation with differential scanner calorimeter (DSC), texture hardness, and shear force. Results show small, but significant changes in physical and visual attributes such as texture and color. No gaping was observed. Only small changes in texture were observed explained by lack of myosin denaturation. The fillets became more white and yellow during storage, whereas the major changes occurred during the 1st week. A panel evaluating QIM and taste could not distinguish major differences in appearance and taste and over 15 d storage period, but were able to quantify the age by smell. Analysis of microorganisms on the epidermis displayed growth of Carnobacterium maltaromaticum, potentially inhibiting growth of other spoilage bacteria. Fish stored for 22 d were rejected by the taste panel caused by a stale smell and taste, but not bitter or rancid. It is concluded that turbot has a shelf life of at least 16 d.
Miguel Hermida - One of the best experts on this subject based on the ideXlab platform.
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Turbot (Scophthalmus Maximus) genomic resources: application for boosting aquaculture production
2016Co-Authors: Paulino Martínez, Diego Robledo, Silvia Téresa Rodriguez Ramilo, Miguel Hermida, Xoana Taboada, Patricia Pereiro, Juan A. Rubiolo, Laia Ribas, Antonio Gómez-tato, Francesc PiferrerAbstract:Turbot (Scophthalmus Maximus) genomic resources: application for boosting aquaculture production
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A microsatellite genetic map of the turbot (Scophthalmus Maximus).
Genetics, 2007Co-Authors: Carmen Bouza, Miguel Hermida, Laura Sánchez, Jaime Castro, Belén G. Pardo, Carlos Fernández, Gloria G. Fortes, Pablo Presa, Montse Pérez, Andrés SanjuanAbstract:A consensus microsatellite-based linkage map of the turbot (Scophthalmus Maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites were mapped in 26 linkage groups, six markers remaining unlinked. The consensus map length was 1343.2 cM, with an average distance between markers of 6.5 ± 0.5 cM. Similar length of female and male maps was evidenced. However, the mean recombination at common intervals throughout the genome revealed significant differences between sexes, ∼1.6 times higher in the female than in the male. The comparison of turbot microsatellite flanking sequences against the Tetraodon nigroviridis genome revealed 55 significant matches, with a mean length of 102 bp and high sequence similarity (81–100%). The comparative mapping revealed significant syntenic regions among fish species. This study represents the first linkage map in the turbot, one of the most important flatfish in European aquaculture. This map will be suitable for QTL identification of productive traits in this species and for further evolutionary studies in fish and vertebrate species.
