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Houssam Attoui - One of the best experts on this subject based on the ideXlab platform.
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Maximum likelihood trees showing phylogenetic comparisons of the aa sequences of VP1 of ORUV, LEBV and CGLV, aligned with those of other Orbivirus species.
2014Co-Authors: Fauziah Mohd Jaafar, Peter P C Mertens, Mourad Belhouchet, Manjunatha Belaganahalli, Robert B. Tesh, Houssam AttouiAbstract:Figure 1 is an ML amino acid tree, respectively, both depicting the three groups of orbiviruses (i-Culicoides−/sandfly-borne, ii- mosquito-borne and iii- tick-borne) as separate clusters. The polymerase of Banna virus (genus Seadornavirus, family Reoviridae: a 12-segmented mosquito-borne dsRNA virus) used as outgroup. This figure shows the root to be located between the tick/tick-borne orbiviruses and the insect-borne orbiviruses. LEBV, ORUV and CGLV all cluster among Culicoides-borne orbiviruses. The scale bar represents the number of substitutions per site.
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Liao ning virus, a new Chinese Seadornavirus that replicates in transformed and embryonic mammalian cells
Journal of General Virology, 2006Co-Authors: Houssam Attoui, Philippe De Micco, Mohd Jaafar Fauziah, Mourad Belhouchet, Sanju Tao, Boquan Chen, Guodong Liang, Robert Tesh, X. De LamballerieAbstract:Seadornaviruses are emerging arboviral pathogens from the south-east of Asia. The genus Seadornavirus contains two distinct species, Banna virus (BAV) isolated from humans with encephalitis and Kadipiro virus. BAV replicates within insect cells and mice but not in cultured mammalian cells. Here, the discovery of Liao ning virus (LNV), a new Seadornavirus from the Aedes dorsalis mosquito, which was completely sequenced and was found to be related to BAV and Kadipiro virus, is reported. Two serotypes of LNV could be distinguished by a serum neutralization assay. According to amino acid identity with other Seadornaviruses, and to criteria set by the ICTV for species delineation, LNV was identified as a member of a new species of virus. Its morphology was characterized by electron microscopy and found to be similar to that of BAV. LNV is the first reported Seadornavirus that replicates in mammalian cells, leading to massive cytopathic effect in all transformed or embryonic cell lines tested. LNV- and BAV-infected mice producing a viraemia lasting for 5 days was followed by viral clearance. Mice infection generated virus quasi-species for LNV (the first reported observation for quasi-species in the family Reoviridae) but not for BAV. Challenge with BAV in mice immunized against BAV did not lead to productive infection. However, challenge with LNV in mice immunized against LNV was lethal with a new phase of viraemia and massive haemorrhage.
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identification and functional analysis of vp3 the guanylyltransferase of banna virus genus Seadornavirus family reoviridae
Journal of General Virology, 2005Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Peter P C Mertens, Philippe De Micco, X. De LamballerieAbstract:Banna virus (BAV) particles contain seven structural proteins: VP4 and VP9 form an outer-capsid layer, whilst the virus core contains three major proteins (VP2, VP8 and VP10) and two minor proteins (VP1 and VP3). Sequence analysis showed that VP3 contains motifs [Kx(I/V/L)S] and (Hx n H) that have previously been identified in the guanylyltransferases of other reoviruses. Incubation of purified BAV-Ch core particles with [α-32P]GTP resulted in exclusive covalent labelling of VP3, demonstrating autoguanylation activity (which is considered indicative of guanylyltransferase activity). Recombinant VP3 prepared in a cell-free expression system was also guanylated under similar reaction conditions, and products were synthesized (in the presence of non-radiolabelled GDP) that co-migrated with GMP, GDP and GpppG during TLC. This reaction, which required magnesium ions for optimum activity, demonstrates that VP3 possesses nucleoside triphosphatase (GTPase) activity and is the BAV guanylyltransferase (RNA ‘capping’ enzyme).
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structural organization of an encephalitic human isolate of banna virus genus Seadornavirus family reoviridae
Journal of General Virology, 2005Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Peter P C Mertens, Philippe De Micco, X. De LamballerieAbstract:Banna virus (BAV) is the type species of the genus Seadornavirus within the family Reoviridae. The Chinese BAV isolate (BAV-Ch), which causes encephalitis in humans, was shown to have a structural organization and particle morphology reminiscent of that of rotaviruses, with fibre proteins projecting from the surface of the particle. Intact BAV-Ch virus particles contain seven structural proteins, two of which (VP4 and VP9) form the outer coat. The inner (core) particles contain five additional proteins (VP1, VP2, VP3, VP8 and VP10) and are ‘non-turreted’, with a relatively smooth surface appearance. VP2 is the ‘T=2’ protein that forms the innermost ‘subcore’ layer, whilst VP8 is the ‘T=13’ protein forming the core-surface layer. Sequence comparisons indicate that BAV VP9 and VP10 are equivalent to the VP8* and VP5* domains, respectively, of rotavirus outer-coat protein VP4 (GenBank accession no. P12976). VP9 has also been shown to be responsible for virus attachment to the host-cell surface and may be involved in internalization. These similarities reveal a previously unreported genetic link between the genera Rotavirus and Seadornavirus, although the expression of BAV VP9 and VP10 from two separate genome segments, rather than by the proteolytic cleavage of a single gene product (as seen in rotavirus VP4), suggests a significant evolutionary jump between the members of these two genera.
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and Xavier de Lamballerie1,4 Correspondence
2005Co-Authors: Houssam AttouiAbstract:Liao ning virus, a new Chinese Seadornavirus that replicates in transformed and embryonic mammalian cell
X. De Lamballerie - One of the best experts on this subject based on the ideXlab platform.
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Liao ning virus, a new Chinese Seadornavirus that replicates in transformed and embryonic mammalian cells
Journal of General Virology, 2006Co-Authors: Houssam Attoui, Philippe De Micco, Mohd Jaafar Fauziah, Mourad Belhouchet, Sanju Tao, Boquan Chen, Guodong Liang, Robert Tesh, X. De LamballerieAbstract:Seadornaviruses are emerging arboviral pathogens from the south-east of Asia. The genus Seadornavirus contains two distinct species, Banna virus (BAV) isolated from humans with encephalitis and Kadipiro virus. BAV replicates within insect cells and mice but not in cultured mammalian cells. Here, the discovery of Liao ning virus (LNV), a new Seadornavirus from the Aedes dorsalis mosquito, which was completely sequenced and was found to be related to BAV and Kadipiro virus, is reported. Two serotypes of LNV could be distinguished by a serum neutralization assay. According to amino acid identity with other Seadornaviruses, and to criteria set by the ICTV for species delineation, LNV was identified as a member of a new species of virus. Its morphology was characterized by electron microscopy and found to be similar to that of BAV. LNV is the first reported Seadornavirus that replicates in mammalian cells, leading to massive cytopathic effect in all transformed or embryonic cell lines tested. LNV- and BAV-infected mice producing a viraemia lasting for 5 days was followed by viral clearance. Mice infection generated virus quasi-species for LNV (the first reported observation for quasi-species in the family Reoviridae) but not for BAV. Challenge with BAV in mice immunized against BAV did not lead to productive infection. However, challenge with LNV in mice immunized against LNV was lethal with a new phase of viraemia and massive haemorrhage.
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identification and functional analysis of vp3 the guanylyltransferase of banna virus genus Seadornavirus family reoviridae
Journal of General Virology, 2005Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Peter P C Mertens, Philippe De Micco, X. De LamballerieAbstract:Banna virus (BAV) particles contain seven structural proteins: VP4 and VP9 form an outer-capsid layer, whilst the virus core contains three major proteins (VP2, VP8 and VP10) and two minor proteins (VP1 and VP3). Sequence analysis showed that VP3 contains motifs [Kx(I/V/L)S] and (Hx n H) that have previously been identified in the guanylyltransferases of other reoviruses. Incubation of purified BAV-Ch core particles with [α-32P]GTP resulted in exclusive covalent labelling of VP3, demonstrating autoguanylation activity (which is considered indicative of guanylyltransferase activity). Recombinant VP3 prepared in a cell-free expression system was also guanylated under similar reaction conditions, and products were synthesized (in the presence of non-radiolabelled GDP) that co-migrated with GMP, GDP and GpppG during TLC. This reaction, which required magnesium ions for optimum activity, demonstrates that VP3 possesses nucleoside triphosphatase (GTPase) activity and is the BAV guanylyltransferase (RNA ‘capping’ enzyme).
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structural organization of an encephalitic human isolate of banna virus genus Seadornavirus family reoviridae
Journal of General Virology, 2005Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Peter P C Mertens, Philippe De Micco, X. De LamballerieAbstract:Banna virus (BAV) is the type species of the genus Seadornavirus within the family Reoviridae. The Chinese BAV isolate (BAV-Ch), which causes encephalitis in humans, was shown to have a structural organization and particle morphology reminiscent of that of rotaviruses, with fibre proteins projecting from the surface of the particle. Intact BAV-Ch virus particles contain seven structural proteins, two of which (VP4 and VP9) form the outer coat. The inner (core) particles contain five additional proteins (VP1, VP2, VP3, VP8 and VP10) and are ‘non-turreted’, with a relatively smooth surface appearance. VP2 is the ‘T=2’ protein that forms the innermost ‘subcore’ layer, whilst VP8 is the ‘T=13’ protein forming the core-surface layer. Sequence comparisons indicate that BAV VP9 and VP10 are equivalent to the VP8* and VP5* domains, respectively, of rotavirus outer-coat protein VP4 (GenBank accession no. P12976). VP9 has also been shown to be responsible for virus attachment to the host-cell surface and may be involved in internalization. These similarities reveal a previously unreported genetic link between the genera Rotavirus and Seadornavirus, although the expression of BAV VP9 and VP10 from two separate genome segments, rather than by the proteolytic cleavage of a single gene product (as seen in rotavirus VP4), suggests a significant evolutionary jump between the members of these two genera.
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Genus Coltivirus (family Reoviridae): genomic and morphologic characterization of Old World and New World viruses
Archives of Virology, 2002Co-Authors: F. Mohd Jaafar, Jean-françois Cantaloube, P. De Micco, P. Biagini, F. A. Murphy, X. De LamballerieAbstract:We report a genomic and morphologic study of the European Eyach (EYA) virus (genus Coltivirus , family Reoviridae ) and a comparative analysis with the American Colorado tick fever (CTF) virus (the type species of the genus). The previously established, but distant, antigenic relationship between these viruses was strengthened by genetic findings (presence of cognate genes, amino acid identity between 55 and 88%, similar conserved terminal motifs, suspected read-through phenomenon in segment 9 of both viruses) and by indistinguishable ultramicroscopic morphologies. Moreover, putative constitutive modifying enzyme activities were suspected to be carried out by homologous viral proteins (RNA-dependent RNA polymerase, methyl/guanylyl transferase, NTPase). These findings, together with the comparative analysis to genomes of south-east Asian isolates, support the recent classification of arboviruses with 12 segments of dsRNA within two distinct genera (genus Coltivirus and genus Seadornavirus ) and raise interesting questions about the evolutionary origins of coltiviruses. The previously proposed hypothesis that EYA virus was derived from an ancestral virus introduced in Europe with the migration of lagomorphs from North-America, would imply a divergence date between American and European isolates of over 50 million years ago (MYA). This analysis allows for the first time to propose an evolutionary rate for virus dsRNA genomes which was found to be in the order of 10^−8 to 10^−9 mutations/nt/year, a rate similar to that of dsDNA genomes.
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Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae.
Journal of General Virology, 2000Co-Authors: Frederique Billoir, P. De Micco, X. De LamballerieAbstract:Arboviruses with genomes composed of 12 segments of double-stranded (ds) RNA have previously been classified as members or probable members of the genus Coltivirus within the family REOVIRIDAE: A number of these viruses have been isolated in North America and Europe and are serologically and genetically related to Colorado tick fever virus, the Coltivirus type species. These isolates constitute subgroup A of the coltiviruses. The complete genome sequences are now presented of two Asian arboviruses, Kadipiro virus (KDV) and Banna virus (BAV), which are currently classified as subgroup B coltiviruses. Analysis of the viral protein sequences shows that all of the BAV genome segments have cognate genes in KDV. The functions of several of these proteins were also indicated by this analysis. Proteins with dsRNA-binding domains or with significant similarities to polymerases, methyltransferases, NTPases or protein kinases were identified. Comparisons of amino acid sequences of the conserved polymerase protein have shown that BAV and KDV are only very distantly related to the subgroup A coltiviruses. These data demonstrate a requirement for the subgroup B viruses to be reassigned to a separate new genus, for which the name Seadornavirus is proposed.
Yunping Man - One of the best experts on this subject based on the ideXlab platform.
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isolation and genetic characterization of mangshi virus a newly discovered Seadornavirus of the reoviridae family found in yunnan province china
PLOS ONE, 2015Co-Authors: Jinglin Wang, Yang Zhou, Jingxing Meng, Wuyang Zhu, Hongyu Chen, Defang Liao, Yunping ManAbstract:Background Seadornavirus is a genus of viruses in the family Reoviridae, which consists of Banna virus, Kadipiro virus, and Liao ning virus. Banna virus is considered a potential pathogen for zoonotic diseases. Here, we describe a newly discovered Seadornavirus isolated from mosquitos (Culex tritaeniorhynchus) in Yunnan Province, China, which is related to Banna virus, and referred to as Mangshi virus.
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Isolation and Genetic Characterization of Mangshi Virus: A Newly Discovered Seadornavirus of the Reoviridae Family Found in Yunnan Province, China.
Public Library of Science (PLoS), 2026Co-Authors: Jinglin Wang, Yang Zhou, Jingxing Meng, Wuyang Zhu, Hongyu Chen, Defang Liao, Yunping ManAbstract:Seadornavirus is a genus of viruses in the family Reoviridae, which consists of Banna virus, Kadipiro virus, and Liao ning virus. Banna virus is considered a potential pathogen for zoonotic diseases. Here, we describe a newly discovered Seadornavirus isolated from mosquitos (Culex tritaeniorhynchus) in Yunnan Province, China, which is related to Banna virus, and referred to as Mangshi virus.The Mangshi virus was isolated by cell culture in Aedes albopictus C6/36 cells, in which it replicated and caused cytopathic effects, but not in mammalian BHK-21 or Vero cells. Polyacrylamide gel analysis revealed a genome consisting of 12 segments of double-stranded RNA, with a "6-4-2" pattern in which the migrating bands were different from those of the Banna virus. Complete genome sequencing was performed by full-length amplification of cDNAs. Sequence analysis showed that seven highly conserved nucleotides and three highly conserved nucleotides were present at the ends of the 5'- and 3'-UTRs in each of 12 genome segments. The amino acid identities of Mangshi virus shared with Balaton virus varied from 27.3% (VP11) to 72.3% (VP1) with Banna virus varying from 18.0% (VP11) to 63.9% (VP1). Phylogenetic analysis based on amino acid sequences demonstrated that Mangshi virus is a member of the genus Seadornavirus and is most closely related to, but distinct from, Balaton virus and Banna virus in the genus Seadornavirus of the family Reoviridae.Mangshi virus isolated from mosquitoes (C. tritaeniorhynchus) was identified as a newly discovered virus in the genus Seadornavirus and is phylogenetically close to Banna virus, suggesting that there is genetic diversity of Seadornaviruses in tropical and subtropical areas of Southeast Asia
Fauziah Mohd Jaafar - One of the best experts on this subject based on the ideXlab platform.
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Isolates of Liao Ning Virus from Wild-Caught Mosquitoes in the Xinjiang Province of China in 2005
2016Co-Authors: Fauziah Mohd Jaafar, Peter P C Mertens, Mourad Belhouchet, Song Zhang, Suxiang Tong, Xiaohong Sun, Guodong LiangAbstract:Liao ning virus (LNV) is related to Banna virus, a known human-pathogen present in south-east Asia. Both viruses belong to the genus Seadornavirus, family Reoviridae. LNV causes lethal haemorrhage in experimentally infected mice. Twenty seven isolates of LNV were made from mosquitoes collected in different locations within the Xinjiang province of north-western China during 2005. These mosquitoes were caught in the accommodation of human patients with febrile manifestations, or in animal barns where sheep represent the main livestock species. The regions where LNV was isolated are affected by seasonal encephalitis, but are free of Japanese encephalitis (JE). Genome segment 10 (Seg-10) (encoding cell-attachment and serotype-determining protein VP10) and Seg-12 (encoding non-structural protein VP12) were sequenced for multiple LNV isolates. Phylogenetic analyses showed a less homogenous Seg-10 gene pool, as compared to segment 12. However, all of these isolates appear to belong to LNV type-1. These data suggest a relatively recent introduction of LNV into Xinjiang province, with substitution rates for LNV Seg-10 and Seg-12, respectively, of 2.2961024 and 1.5761024 substitutions/nt/ year. These substitution rates are similar to those estimated for other dsRNA viruses. Our data indicate that the history of LNV is characterized by a lack of demographic fluctuations. However, a decline in the LNV population in the late 1980s-early 1990s, was indicated by data for both Seg-10 and Seg-12. Data also suggest a beginning of an expansion in the lat
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Maximum likelihood trees showing phylogenetic comparisons of the aa sequences of VP1 of ORUV, LEBV and CGLV, aligned with those of other Orbivirus species.
2014Co-Authors: Fauziah Mohd Jaafar, Peter P C Mertens, Mourad Belhouchet, Manjunatha Belaganahalli, Robert B. Tesh, Houssam AttouiAbstract:Figure 1 is an ML amino acid tree, respectively, both depicting the three groups of orbiviruses (i-Culicoides−/sandfly-borne, ii- mosquito-borne and iii- tick-borne) as separate clusters. The polymerase of Banna virus (genus Seadornavirus, family Reoviridae: a 12-segmented mosquito-borne dsRNA virus) used as outgroup. This figure shows the root to be located between the tick/tick-borne orbiviruses and the insect-borne orbiviruses. LEBV, ORUV and CGLV all cluster among Culicoides-borne orbiviruses. The scale bar represents the number of substitutions per site.
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identification and functional analysis of vp3 the guanylyltransferase of banna virus genus Seadornavirus family reoviridae
Journal of General Virology, 2005Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Peter P C Mertens, Philippe De Micco, X. De LamballerieAbstract:Banna virus (BAV) particles contain seven structural proteins: VP4 and VP9 form an outer-capsid layer, whilst the virus core contains three major proteins (VP2, VP8 and VP10) and two minor proteins (VP1 and VP3). Sequence analysis showed that VP3 contains motifs [Kx(I/V/L)S] and (Hx n H) that have previously been identified in the guanylyltransferases of other reoviruses. Incubation of purified BAV-Ch core particles with [α-32P]GTP resulted in exclusive covalent labelling of VP3, demonstrating autoguanylation activity (which is considered indicative of guanylyltransferase activity). Recombinant VP3 prepared in a cell-free expression system was also guanylated under similar reaction conditions, and products were synthesized (in the presence of non-radiolabelled GDP) that co-migrated with GMP, GDP and GpppG during TLC. This reaction, which required magnesium ions for optimum activity, demonstrates that VP3 possesses nucleoside triphosphatase (GTPase) activity and is the BAV guanylyltransferase (RNA ‘capping’ enzyme).
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structural organization of an encephalitic human isolate of banna virus genus Seadornavirus family reoviridae
Journal of General Virology, 2005Co-Authors: Fauziah Mohd Jaafar, Houssam Attoui, Peter P C Mertens, Philippe De Micco, X. De LamballerieAbstract:Banna virus (BAV) is the type species of the genus Seadornavirus within the family Reoviridae. The Chinese BAV isolate (BAV-Ch), which causes encephalitis in humans, was shown to have a structural organization and particle morphology reminiscent of that of rotaviruses, with fibre proteins projecting from the surface of the particle. Intact BAV-Ch virus particles contain seven structural proteins, two of which (VP4 and VP9) form the outer coat. The inner (core) particles contain five additional proteins (VP1, VP2, VP3, VP8 and VP10) and are ‘non-turreted’, with a relatively smooth surface appearance. VP2 is the ‘T=2’ protein that forms the innermost ‘subcore’ layer, whilst VP8 is the ‘T=13’ protein forming the core-surface layer. Sequence comparisons indicate that BAV VP9 and VP10 are equivalent to the VP8* and VP5* domains, respectively, of rotavirus outer-coat protein VP4 (GenBank accession no. P12976). VP9 has also been shown to be responsible for virus attachment to the host-cell surface and may be involved in internalization. These similarities reveal a previously unreported genetic link between the genera Rotavirus and Seadornavirus, although the expression of BAV VP9 and VP10 from two separate genome segments, rather than by the proteolytic cleavage of a single gene product (as seen in rotavirus VP4), suggests a significant evolutionary jump between the members of these two genera.
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study of proteins of colorado tick fever eyach and banna viruses application in serological diagnosis and the structural characterization of virions
2004Co-Authors: Fauziah Mohd JaafarAbstract:Cette these rapport l'etude de deux groupes d'arbovirus de la famille de Reoviridae qui appartiennent aux genres Coltivirus et Seadornavirus. Les proteines recombinantes de ces virus ont ete exprimees dans des systemes bacteriens dans le but d'etablir des tests serologiques pour le diagnostic de l'infection. Des anticorps diriges contre les proteines recombinantes ont servi dans l'identification des proteines structurales et non structurales du banna virus, un virus responsable d'encephalites chez les humains. Nous avons determine le nombre de copie des proteines structurales de ce virus. La structure de la proteine de la capside externe du Banna virus a ete determinee par diffraction aux rayons X a la resolution de 2. 6Aʿ. Cette meme proteine porte les epitopes seroneutralisant. L'attachement du virus ne necessite pas la presence d'acide sialique. La pre-incubation des cellules avec la VP9 trimeric augmente considerablement l'infectiosite du virus. Les activites de la guanylyltransferase virale ainsi qu'une proteine d'attachement a l'ARN bicatenaire ont ete etudies pour le Banna virus. D'autre part, la lecture a travers un codon stop, suspecte chez le virus de la fievre du Colorado a ete confirme experimentalement
Jinglin Wang - One of the best experts on this subject based on the ideXlab platform.
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isolation and genetic characterization of mangshi virus a newly discovered Seadornavirus of the reoviridae family found in yunnan province china
PLOS ONE, 2015Co-Authors: Jinglin Wang, Yang Zhou, Jingxing Meng, Wuyang Zhu, Hongyu Chen, Defang Liao, Yunping ManAbstract:Background Seadornavirus is a genus of viruses in the family Reoviridae, which consists of Banna virus, Kadipiro virus, and Liao ning virus. Banna virus is considered a potential pathogen for zoonotic diseases. Here, we describe a newly discovered Seadornavirus isolated from mosquitos (Culex tritaeniorhynchus) in Yunnan Province, China, which is related to Banna virus, and referred to as Mangshi virus.
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Isolation and Genetic Characterization of Mangshi Virus: A Newly Discovered Seadornavirus of the Reoviridae Family Found in Yunnan Province, China.
Public Library of Science (PLoS), 2026Co-Authors: Jinglin Wang, Yang Zhou, Jingxing Meng, Wuyang Zhu, Hongyu Chen, Defang Liao, Yunping ManAbstract:Seadornavirus is a genus of viruses in the family Reoviridae, which consists of Banna virus, Kadipiro virus, and Liao ning virus. Banna virus is considered a potential pathogen for zoonotic diseases. Here, we describe a newly discovered Seadornavirus isolated from mosquitos (Culex tritaeniorhynchus) in Yunnan Province, China, which is related to Banna virus, and referred to as Mangshi virus.The Mangshi virus was isolated by cell culture in Aedes albopictus C6/36 cells, in which it replicated and caused cytopathic effects, but not in mammalian BHK-21 or Vero cells. Polyacrylamide gel analysis revealed a genome consisting of 12 segments of double-stranded RNA, with a "6-4-2" pattern in which the migrating bands were different from those of the Banna virus. Complete genome sequencing was performed by full-length amplification of cDNAs. Sequence analysis showed that seven highly conserved nucleotides and three highly conserved nucleotides were present at the ends of the 5'- and 3'-UTRs in each of 12 genome segments. The amino acid identities of Mangshi virus shared with Balaton virus varied from 27.3% (VP11) to 72.3% (VP1) with Banna virus varying from 18.0% (VP11) to 63.9% (VP1). Phylogenetic analysis based on amino acid sequences demonstrated that Mangshi virus is a member of the genus Seadornavirus and is most closely related to, but distinct from, Balaton virus and Banna virus in the genus Seadornavirus of the family Reoviridae.Mangshi virus isolated from mosquitoes (C. tritaeniorhynchus) was identified as a newly discovered virus in the genus Seadornavirus and is phylogenetically close to Banna virus, suggesting that there is genetic diversity of Seadornaviruses in tropical and subtropical areas of Southeast Asia