The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
Deli Dong - One of the best experts on this subject based on the ideXlab platform.
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A poly(glycerol-Sebacate-curcumin) polymer with potential use for brain gliomas.
Journal of biomedical materials research. Part A, 2012Co-Authors: Sun Zhijie, Bo Sun, Rong-bin Tao, Xin Xie, Lu Xili, Deli DongAbstract:Curcumin has multiple biological and pharmacological activities, including antioxidant, anti-inflammatory, antiviral, antibacterial, antifungal, and antitumor activities. However, the clinical use of curcumin is limited because of its poor oral absorption and extremely poor bioavailability. In order to overcome these limitations, we conjugate curcumin chemically into the known biocompatible and biodegradable polymer, poly(glycerol–Sebacate), and prepare the unitary poly(glycerol–Sebacate–curcumin) polymer. The structure, the in vitro degradation, the drug release, and antitumor activity as well as the in vivo degradation and tissue biocompatibility of poly(glycerol–Sebacate–curcumin) polymer are investigated. The in vitro degradation and drug release profile of poly(glycerol–Sebacate–curcumin) are in a linear manner. The in vitro antitumor assay shows that poly(glycerol–Sebacate–curcumin) polymer significantly inhibits human malignant glioma cells, U87 and T98 cells. In view of the cytotoxicity against brain gliomas, local use of this polymer would be a potential method for brain tumors. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A:253–260, 2013.
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A poly(glycerol-Sebacate-(5-fluorouracil-1-acetic acid)) polymer with potential use for cancer therapy
Journal of Bioactive and Compatible Polymers, 2012Co-Authors: Sun Zhijie, Bo Sun, Xin Xie, Lu Xili, Cheng-wu Sun, Li-bo Wang, Deli DongAbstract:In this study, 5-fluorouracil-1-acetic acid was chemically conjugated with poly(glycerol-Sebacate) (PGS) to form a unitary polymer poly(glycerol-Sebacate- (5-fluorouracil-1-acetic acid)) (PGS-5-FU-...
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glycolic acid modulates the mechanical property and degradation of poly glycerol Sebacate glycolic acid
Journal of Biomedical Materials Research Part A, 2010Co-Authors: Lan Wu, Wei Huang, Chang Chen, Yan Chen, Xili Lu, Xiaolan Zhang, Baofeng Yang, Deli DongAbstract:The development of biodegradable materials with controllable degradation properties is beneficial for a variety of applications. Poly(glycerol-Sebacate) (PGS) is a promising candidate of biomaterials; so we synthesize a series of poly(glycerol, Sebacate, glycolic acid) (PGSG) with 1:2:0, 1:2:0.2, 1:2:0.4, 1:2:0.6, 1:2:1 mole ratio of glycerol, Sebacate, and glycolic acid to elucidate the relation of doped glycolic acid to the degradation rate and mechanical properties. The microstructures of the polymers with different doping of glycolic acid were dissimilar. PGSG with glycolic acid in the ratio of 0.2 displayed an integral degree of ordering, different to those with glycolic acid in the ratio of 0, 0.4, 0.6, and 1, which showed mild phase separation structure. The number, ΔHm, and temperature of the PGSG melting peaks tended to decrease with the increasing ratio of doped glycolic acid. In vitro and in vivo degradation tests showed that the degradation rate of PGSG with glycolic acid in the ratio of 0.2 was slowest, but in the ratio range of 0, 0.4, and 0.6, the degradation rate increased with the increase of glycolic acid. All PGSG samples displayed good tissue response and anticoagulant effects. Our data suggest that doping glycolic acid can modulate the microstructure and degree of crosslinking of PGS, thereby control the degradation rate of PGS. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
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The influence of lactic on the properties of Poly (glycerol–Sebacate–lactic acid)
Materials Science and Engineering: C, 2009Co-Authors: Sun Zhijie, Wei Huang, Xiaolan Zhang, Baofeng Yang, Lu Xili, Yufeng Zheng, Deli DongAbstract:Abstract We synthesized a series of poly (glycerol, Sebacate and lactic acid) (PGSL) with 1:1:0, 1:1:0.25, 1:1:0.5, 1:1:1 mole ratio of glycerol, Sebacate and lactic acid, in order to elucidate the relation of microstructure to the degradation rate and mechanical properties. The microstructure of the polymer with lactic acid in the ratio of 0.25 displayed phase separation structure. The crystallization temperature ( T c ) and absolute crystallization enthalpy (Δ H c ) of PGSL tended to decrease with the increasing ratio of lactic acid. Degradation rate of PGSL with lactic acid in the ratio of 0.25 was fastest in vitro and 35% mass loss occurred after 60 day degradation. In the range of 0, 0.5 and 1, the degradation rate decreased slightly with the lactic acid increasing and 18% mass loss occurred after 60 day degradation when lactic acid was doped in the ratio of 1.0. All PGSL polymers inhibited platelet adhesion, prolonged whole blood clotting time, activated partial thromboplastin time and prothrombin time. In conclusion, doping lactic acid can modulate the microstructure of poly (glycerol, Sebacate) (PGS), thereby control the degradation rate and mechanical property of PGS.
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the influence of lactic on the properties of poly glycerol Sebacate lactic acid
Materials Science and Engineering: C, 2009Co-Authors: Zhijie Sun, Wei Huang, Xiaolan Zhang, Baofeng Yang, Yufeng Zheng, Deli DongAbstract:Abstract We synthesized a series of poly (glycerol, Sebacate and lactic acid) (PGSL) with 1:1:0, 1:1:0.25, 1:1:0.5, 1:1:1 mole ratio of glycerol, Sebacate and lactic acid, in order to elucidate the relation of microstructure to the degradation rate and mechanical properties. The microstructure of the polymer with lactic acid in the ratio of 0.25 displayed phase separation structure. The crystallization temperature ( T c ) and absolute crystallization enthalpy (Δ H c ) of PGSL tended to decrease with the increasing ratio of lactic acid. Degradation rate of PGSL with lactic acid in the ratio of 0.25 was fastest in vitro and 35% mass loss occurred after 60 day degradation. In the range of 0, 0.5 and 1, the degradation rate decreased slightly with the lactic acid increasing and 18% mass loss occurred after 60 day degradation when lactic acid was doped in the ratio of 1.0. All PGSL polymers inhibited platelet adhesion, prolonged whole blood clotting time, activated partial thromboplastin time and prothrombin time. In conclusion, doping lactic acid can modulate the microstructure of poly (glycerol, Sebacate) (PGS), thereby control the degradation rate and mechanical property of PGS.
Davod Mohebbi-kalhori - One of the best experts on this subject based on the ideXlab platform.
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Polyglycerol Sebacate/chitosan/gelatin nano-composite scaffolds for engineering neural construct
Materials Chemistry and Physics, 2019Co-Authors: Sanaz Saravani, Mehdi Ebrahimian-hosseinabadi, Davod Mohebbi-kalhoriAbstract:Abstract The aim of this study was designing and fabricating nano-composite scaffolds based on polyglycerol Sebacate in order to use in nerve tissue engineering. Semi-crystalline polyglycerol Sebacate polymer was synthesized and evaluated by X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) methods. The synthesized polyglycerol Sebacate was electrospun with chitosan and gelatin at different concentrations under various conditions. The obtained samples were examined using Scanning electron microscopy (SEM) observations; the diameters of fibers were measured via image processing method, and the percentage of scaffolds porosity were obtained as well. Results demonstrated that semi-crystalline polyglycerol Sebacate/chitosan/gelatin could be electrospun to produce fibers with the diameter of about 80 nm in average. MTT assay were performed using PC12 cell line; after 3 days of cell culture, it seems that polyglycerol Sebacate/Chitosan/Gelatin nano-composite has potentiality for nerve tissue engineering applications.
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Polyglycerol Sebacate/chitosan/gelatin nano-composite scaffolds for engineering neural construct
Materials Chemistry and Physics, 2019Co-Authors: Sanaz Saravani, Mehdi Ebrahimian-hosseinabadi, Davod Mohebbi-kalhoriAbstract:Abstract The aim of this study was designing and fabricating nano-composite scaffolds based on polyglycerol Sebacate in order to use in nerve tissue engineering. Semi-crystalline polyglycerol Sebacate polymer was synthesized and evaluated by X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) methods. The synthesized polyglycerol Sebacate was electrospun with chitosan and gelatin at different concentrations under various conditions. The obtained samples were examined using Scanning electron microscopy (SEM) observations; the diameters of fibers were measured via image processing method, and the percentage of scaffolds porosity were obtained as well. Results demonstrated that semi-crystalline polyglycerol Sebacate/chitosan/gelatin could be electrospun to produce fibers with the diameter of about 80 nm in average. MTT assay were performed using PC12 cell line; after 3 days of cell culture, it seems that polyglycerol Sebacate/Chitosan/Gelatin nano-composite has potentiality for nerve tissue engineering applications.
Mehdi Ebrahimian-hosseinabadi - One of the best experts on this subject based on the ideXlab platform.
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Polyglycerol Sebacate/chitosan/gelatin nano-composite scaffolds for engineering neural construct
Materials Chemistry and Physics, 2019Co-Authors: Sanaz Saravani, Mehdi Ebrahimian-hosseinabadi, Davod Mohebbi-kalhoriAbstract:Abstract The aim of this study was designing and fabricating nano-composite scaffolds based on polyglycerol Sebacate in order to use in nerve tissue engineering. Semi-crystalline polyglycerol Sebacate polymer was synthesized and evaluated by X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) methods. The synthesized polyglycerol Sebacate was electrospun with chitosan and gelatin at different concentrations under various conditions. The obtained samples were examined using Scanning electron microscopy (SEM) observations; the diameters of fibers were measured via image processing method, and the percentage of scaffolds porosity were obtained as well. Results demonstrated that semi-crystalline polyglycerol Sebacate/chitosan/gelatin could be electrospun to produce fibers with the diameter of about 80 nm in average. MTT assay were performed using PC12 cell line; after 3 days of cell culture, it seems that polyglycerol Sebacate/Chitosan/Gelatin nano-composite has potentiality for nerve tissue engineering applications.
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Polyglycerol Sebacate/chitosan/gelatin nano-composite scaffolds for engineering neural construct
Materials Chemistry and Physics, 2019Co-Authors: Sanaz Saravani, Mehdi Ebrahimian-hosseinabadi, Davod Mohebbi-kalhoriAbstract:Abstract The aim of this study was designing and fabricating nano-composite scaffolds based on polyglycerol Sebacate in order to use in nerve tissue engineering. Semi-crystalline polyglycerol Sebacate polymer was synthesized and evaluated by X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) methods. The synthesized polyglycerol Sebacate was electrospun with chitosan and gelatin at different concentrations under various conditions. The obtained samples were examined using Scanning electron microscopy (SEM) observations; the diameters of fibers were measured via image processing method, and the percentage of scaffolds porosity were obtained as well. Results demonstrated that semi-crystalline polyglycerol Sebacate/chitosan/gelatin could be electrospun to produce fibers with the diameter of about 80 nm in average. MTT assay were performed using PC12 cell line; after 3 days of cell culture, it seems that polyglycerol Sebacate/Chitosan/Gelatin nano-composite has potentiality for nerve tissue engineering applications.
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Poly(glycerol-Sebacate)/poly(caprolactone)/graphene nanocomposites for nerve tissue engineering:
Journal of Bioactive and Compatible Polymers, 2018Co-Authors: Shirin Ghafaralahi, Mehdi Ebrahimian-hosseinabadi, Anousheh Zargar KharaziAbstract:In this study, mechanical, electrical, physical, and biological properties of polymeric matrixes comprising poly(glycerol-Sebacate) (PGS) and poly(caprolactone) (PCL) with various weight ratio of P...
Sanaz Saravani - One of the best experts on this subject based on the ideXlab platform.
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Polyglycerol Sebacate/chitosan/gelatin nano-composite scaffolds for engineering neural construct
Materials Chemistry and Physics, 2019Co-Authors: Sanaz Saravani, Mehdi Ebrahimian-hosseinabadi, Davod Mohebbi-kalhoriAbstract:Abstract The aim of this study was designing and fabricating nano-composite scaffolds based on polyglycerol Sebacate in order to use in nerve tissue engineering. Semi-crystalline polyglycerol Sebacate polymer was synthesized and evaluated by X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) methods. The synthesized polyglycerol Sebacate was electrospun with chitosan and gelatin at different concentrations under various conditions. The obtained samples were examined using Scanning electron microscopy (SEM) observations; the diameters of fibers were measured via image processing method, and the percentage of scaffolds porosity were obtained as well. Results demonstrated that semi-crystalline polyglycerol Sebacate/chitosan/gelatin could be electrospun to produce fibers with the diameter of about 80 nm in average. MTT assay were performed using PC12 cell line; after 3 days of cell culture, it seems that polyglycerol Sebacate/Chitosan/Gelatin nano-composite has potentiality for nerve tissue engineering applications.
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Polyglycerol Sebacate/chitosan/gelatin nano-composite scaffolds for engineering neural construct
Materials Chemistry and Physics, 2019Co-Authors: Sanaz Saravani, Mehdi Ebrahimian-hosseinabadi, Davod Mohebbi-kalhoriAbstract:Abstract The aim of this study was designing and fabricating nano-composite scaffolds based on polyglycerol Sebacate in order to use in nerve tissue engineering. Semi-crystalline polyglycerol Sebacate polymer was synthesized and evaluated by X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) methods. The synthesized polyglycerol Sebacate was electrospun with chitosan and gelatin at different concentrations under various conditions. The obtained samples were examined using Scanning electron microscopy (SEM) observations; the diameters of fibers were measured via image processing method, and the percentage of scaffolds porosity were obtained as well. Results demonstrated that semi-crystalline polyglycerol Sebacate/chitosan/gelatin could be electrospun to produce fibers with the diameter of about 80 nm in average. MTT assay were performed using PC12 cell line; after 3 days of cell culture, it seems that polyglycerol Sebacate/Chitosan/Gelatin nano-composite has potentiality for nerve tissue engineering applications.
George C Engelmayr - One of the best experts on this subject based on the ideXlab platform.
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design and testing of a cyclic stretch and flexure bioreactor for evaluating engineered heart valve tissues based on poly glycerol Sebacate scaffolds
Proceedings of the Institution of Mechanical Engineers Part H: Journal of Engineering in Medicine, 2014Co-Authors: Nafiseh Masoumi, Christian M Howell, Katherine L Johnson, Matthew J Niesslein, Gene Gerber, George C EngelmayrAbstract:Cyclic flexure and stretch are essential to the function of semilunar heart valves and have demonstrated utility in mechanically conditioning tissue-engineered heart valves. In this study, a cyclic stretch and flexure bioreactor was designed and tested in the context of the bioresorbable elastomer poly(glycerol Sebacate). Solid poly(glycerol Sebacate) membranes were subjected to cyclic stretch, and micromolded poly(glycerol Sebacate) scaffolds seeded with porcine aortic valvular interstitial cells were subjected to cyclic stretch and flexure. The results demonstrated significant effects of cyclic stretch on poly(glycerol Sebacate) mechanical properties, including significant decreases in effective stiffness versus controls. In valvular interstitial cell-seeded scaffolds, cyclic stretch elicited significant increases in DNA and collagen content that paralleled maintenance of effective stiffness. This work provides a basis for investigating the roles of mechanical loading in the formation of tissue-engineer...
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Design and testing of a cyclic stretch and flexure bioreactor for evaluating engineered heart valve tissues based on poly(glycerol Sebacate) scaffolds.
Proceedings of the Institution of Mechanical Engineers. Part H Journal of engineering in medicine, 2014Co-Authors: Nafiseh Masoumi, Katherine L Johnson, Matthew J Niesslein, Gene Gerber, M. Christian Howell, George C EngelmayrAbstract:Cyclic flexure and stretch are essential to the function of semilunar heart valves and have demonstrated utility in mechanically conditioning tissue-engineered heart valves. In this study, a cyclic stretch and flexure bioreactor was designed and tested in the context of the bioresorbable elastomer poly(glycerol Sebacate). Solid poly(glycerol Sebacate) membranes were subjected to cyclic stretch, and micromolded poly(glycerol Sebacate) scaffolds seeded with porcine aortic valvular interstitial cells were subjected to cyclic stretch and flexure. The results demonstrated significant effects of cyclic stretch on poly(glycerol Sebacate) mechanical properties, including significant decreases in effective stiffness versus controls. In valvular interstitial cell-seeded scaffolds, cyclic stretch elicited significant increases in DNA and collagen content that paralleled maintenance of effective stiffness. This work provides a basis for investigating the roles of mechanical loading in the formation of tissue-engineered heart valves based on elastomeric scaffolds.
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valvular interstitial cell seeded poly glycerol Sebacate scaffolds toward a biomimetic in vitro model for heart valve tissue engineering
Acta Biomaterialia, 2013Co-Authors: Nafiseh Masoumi, Christian M Howell, Katherine L Johnson, George C EngelmayrAbstract:Abstract Tissue engineered replacement heart valves may be capable of overcoming the lack of growth potential intrinsic to current non-viable prosthetics, and thus could potentially serve as permanent replacements in the surgical repair of pediatric valvular lesions. However, the evaluation of candidate combinations of cells and scaffolds lacks a biomimetic in vitro model with broadly tunable, anisotropic and elastomeric structural–mechanical properties. Toward establishing such an in vitro model, in the current study, porcine aortic and pulmonary valvular interstitial cells (i.e. biomimetic cells) were cultivated on anisotropic, micromolded poly(glycerol Sebacate) scaffolds (i.e. biomimetic scaffolds). Following 14 and 28 days of static culture, cell-seeded scaffolds and unseeded controls were assessed for their mechanical properties, and cell-seeded scaffolds were further characterized by confocal fluorescence and scanning electron microscopy, and by collagen and DNA assays. Poly(glycerol Sebacate) micromolding yielded scaffolds with anisotropic stiffnesses resembling those of native valvular tissues in the low stress–strain ranges characteristic of physiologic valvular function. Scaffold anisotropy was largely retained upon cultivation with valvular interstitial cells; while the mechanical properties of unseeded scaffolds progressively diminished, cell-seeded scaffolds either retained or exceeded initial mechanical properties. Retention of mechanical properties in cell-seeded scaffolds paralleled the accretion of collagen, which increased significantly from 14 to 28 days. This study demonstrates that valvular interstitial cells can be cultivated on anisotropic poly(glycerol Sebacate) scaffolds to yield biomimetic in vitro models with which clinically relevant cells and future scaffold designs can be evaluated.
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protein precoating of elastomeric tissue engineering scaffolds increased cellularity enhanced extracellular matrix protein production and differentially regulated the phenotypes of circulating endothelial progenitor cells
Circulation, 2007Co-Authors: Virna L Sales, George C Engelmayr, John Asher Johnson, Yadong Wang, Michael S Sacks, John E MayerAbstract:Background— Optimal cell sources and scaffold-cell interactions remain unanswered questions for tissue engineering of heart valves. We assessed the effect of different protein precoatings on a single scaffold type (elastomeric poly (glycerol Sebacate)) with a single cell source (endothelial progenitor cells). Methods and Results— Elastomeric poly (glycerol Sebacate) scaffolds were precoated with laminin, fibronectin, fibrin, collagen types I/III, or elastin. Characterized ovine peripheral blood endothelial progenitor cells were seeded onto scaffolds for 3 days followed by 14 days incubation. Endothelial progenitor cells were CD31+, vWF+, and α-SMA− before seeding confirmed by immunohistochemistry and immunoblotting. Both precoated and uncoated scaffolds demonstrated surface expression of CD31+ and vWF+, α-SMA+ cells and were found in the “interstitium” of the scaffold. Protein precoating of elastomeric poly (glycerol Sebacate) scaffolds revealed significantly increased cellularity and altered the phenotypes of endothelial progenitor cells, which resulted in changes in cellular behavior and extracellular matrix production. Moreover, mechanical flexure testing demonstrated decreased effective stiffness of the seeded scaffolds compared with unseeded controls. Conclusions— Scaffold precoating with extracellular matrix proteins can allow more precise “engineering” of cellular behavior in the development of tissue engineering of heart valves constructs by altering extracellular matrix production and cell phenotype.
