The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
Ronald R Breaker - One of the best experts on this subject based on the ideXlab platform.
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riboswitches in eubacteria sense the Second Messenger c di amp
Nature Chemical Biology, 2013Co-Authors: James W Nelson, Zasha Weinberg, N. Sudarsan, Kazuhiro Furukawa, Joy Xin Wang, Ronald R BreakerAbstract:Cyclic diadenosine monophosphate (c-di-AMP) is a newly identified nucleotide Second Messenger in bacteria. Though protein receptors for c-di-AMP are known, the ydaO riboswitch has now been validated as a physiological sensor of cellular c-di-AMP levels.
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riboswitches in eubacteria sense the Second Messenger c di amp
Nature Chemical Biology, 2013Co-Authors: James W Nelson, Zasha Weinberg, N. Sudarsan, Kazuhiro Furukawa, Joy Xin Wang, Ronald R BreakerAbstract:Cyclic di-adenosine monophosphate (c-di-AMP) is a recently discovered bacterial Second Messenger implicated in the control of cell wall metabolism, osmotic stress responses and sporulation. However, the mechanisms by which c-di-AMP triggers these physiological responses have remained largely unknown. Notably, a candidate riboswitch class called ydaO associates with numerous genes involved in these same processes. Although a representative ydaO motif RNA recently was reported to weakly bind ATP, we report that numerous members of this noncoding RNA class selectively respond to c-di-AMP with subnanomolar affinity. Our findings resolve the mystery regarding the primary ligand for this extremely common riboswitch class and expose a major portion of the super-regulon of genes that are controlled by the widespread bacterial Second Messenger c-di-AMP.
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engineered allosteric ribozymes that sense the bacterial Second Messenger cyclic diguanosyl 5 monophosphate
Analytical Chemistry, 2012Co-Authors: Kazuhiro Furukawa, Ronald R BreakerAbstract:A series of allosteric ribozymes that respond to the bacterial Second Messenger cyclic diguanosyl-5′-monophosphate (c-di-GMP) have been created by using in vitro selection. An RNA library was gener...
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an allosteric self splicing ribozyme triggered by a bacterial Second Messenger
Science, 2010Co-Authors: Elaine R Lee, Zasha Weinberg, N. Sudarsan, Ronald R Breaker, Jenny L BakerAbstract:Group I self-splicing ribozymes commonly function as components of selfish mobile genetic elements. We identified an allosteric group I ribozyme, wherein self-splicing is regulated by a distinct riboswitch class that senses the bacterial Second Messenger c-di-GMP. The tandem RNA sensory system resides in the 5' untranslated region of the Messenger RNA for a putative virulence gene in the pathogenic bacterium Clostridium difficile. c-di-GMP binding by the riboswitch induces folding changes at atypical splice site junctions to modulate alternative RNA processing. Our findings indicate that some self-splicing ribozymes are not selfish elements but are harnessed by cells as metabolite sensors and genetic regulators.
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Riboswitches in eubacteria sense the Second Messenger cyclic Di-GMP
Science, 2008Co-Authors: N. Sudarsan, E. R. Lee, Zasha Weinberg, R. H. Moy, K. H. Link, Ronald R BreakerAbstract:Cyclic di-guanosine monophosphate (di-GMP) is a circular RNA dinucleotide that functions as a Second Messenger in diverse species of bacteria to trigger wide-ranging physiological changes, including cell differentiation, conversion between motile and biofilm lifestyles, and virulence gene expression. However, the mechanisms by which cyclic di-GMP regulates gene expression have remained a mystery. We found that cyclic di-GMP in many bacterial species is sensed by a riboswitch class in Messenger RNA that controls the expression of genes involved in numerous fundamental cellular processes. A variety of cyclic di-GMP regulons are revealed, including some riboswitches associated with virulence gene expression, pilus formation, and flagellum biosynthesis. In addition, sequences matching the consensus for cyclic di-GMP riboswitches are present in the genome of a bacteriophage.
N. Sudarsan - One of the best experts on this subject based on the ideXlab platform.
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riboswitches in eubacteria sense the Second Messenger c di amp
Nature Chemical Biology, 2013Co-Authors: James W Nelson, Zasha Weinberg, N. Sudarsan, Kazuhiro Furukawa, Joy Xin Wang, Ronald R BreakerAbstract:Cyclic diadenosine monophosphate (c-di-AMP) is a newly identified nucleotide Second Messenger in bacteria. Though protein receptors for c-di-AMP are known, the ydaO riboswitch has now been validated as a physiological sensor of cellular c-di-AMP levels.
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riboswitches in eubacteria sense the Second Messenger c di amp
Nature Chemical Biology, 2013Co-Authors: James W Nelson, Zasha Weinberg, N. Sudarsan, Kazuhiro Furukawa, Joy Xin Wang, Ronald R BreakerAbstract:Cyclic di-adenosine monophosphate (c-di-AMP) is a recently discovered bacterial Second Messenger implicated in the control of cell wall metabolism, osmotic stress responses and sporulation. However, the mechanisms by which c-di-AMP triggers these physiological responses have remained largely unknown. Notably, a candidate riboswitch class called ydaO associates with numerous genes involved in these same processes. Although a representative ydaO motif RNA recently was reported to weakly bind ATP, we report that numerous members of this noncoding RNA class selectively respond to c-di-AMP with subnanomolar affinity. Our findings resolve the mystery regarding the primary ligand for this extremely common riboswitch class and expose a major portion of the super-regulon of genes that are controlled by the widespread bacterial Second Messenger c-di-AMP.
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an allosteric self splicing ribozyme triggered by a bacterial Second Messenger
Science, 2010Co-Authors: Elaine R Lee, Zasha Weinberg, N. Sudarsan, Ronald R Breaker, Jenny L BakerAbstract:Group I self-splicing ribozymes commonly function as components of selfish mobile genetic elements. We identified an allosteric group I ribozyme, wherein self-splicing is regulated by a distinct riboswitch class that senses the bacterial Second Messenger c-di-GMP. The tandem RNA sensory system resides in the 5' untranslated region of the Messenger RNA for a putative virulence gene in the pathogenic bacterium Clostridium difficile. c-di-GMP binding by the riboswitch induces folding changes at atypical splice site junctions to modulate alternative RNA processing. Our findings indicate that some self-splicing ribozymes are not selfish elements but are harnessed by cells as metabolite sensors and genetic regulators.
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Riboswitches in eubacteria sense the Second Messenger cyclic Di-GMP
Science, 2008Co-Authors: N. Sudarsan, E. R. Lee, Zasha Weinberg, R. H. Moy, K. H. Link, Ronald R BreakerAbstract:Cyclic di-guanosine monophosphate (di-GMP) is a circular RNA dinucleotide that functions as a Second Messenger in diverse species of bacteria to trigger wide-ranging physiological changes, including cell differentiation, conversion between motile and biofilm lifestyles, and virulence gene expression. However, the mechanisms by which cyclic di-GMP regulates gene expression have remained a mystery. We found that cyclic di-GMP in many bacterial species is sensed by a riboswitch class in Messenger RNA that controls the expression of genes involved in numerous fundamental cellular processes. A variety of cyclic di-GMP regulons are revealed, including some riboswitches associated with virulence gene expression, pilus formation, and flagellum biosynthesis. In addition, sequences matching the consensus for cyclic di-GMP riboswitches are present in the genome of a bacteriophage.
Zasha Weinberg - One of the best experts on this subject based on the ideXlab platform.
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riboswitches in eubacteria sense the Second Messenger c di amp
Nature Chemical Biology, 2013Co-Authors: James W Nelson, Zasha Weinberg, N. Sudarsan, Kazuhiro Furukawa, Joy Xin Wang, Ronald R BreakerAbstract:Cyclic diadenosine monophosphate (c-di-AMP) is a newly identified nucleotide Second Messenger in bacteria. Though protein receptors for c-di-AMP are known, the ydaO riboswitch has now been validated as a physiological sensor of cellular c-di-AMP levels.
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riboswitches in eubacteria sense the Second Messenger c di amp
Nature Chemical Biology, 2013Co-Authors: James W Nelson, Zasha Weinberg, N. Sudarsan, Kazuhiro Furukawa, Joy Xin Wang, Ronald R BreakerAbstract:Cyclic di-adenosine monophosphate (c-di-AMP) is a recently discovered bacterial Second Messenger implicated in the control of cell wall metabolism, osmotic stress responses and sporulation. However, the mechanisms by which c-di-AMP triggers these physiological responses have remained largely unknown. Notably, a candidate riboswitch class called ydaO associates with numerous genes involved in these same processes. Although a representative ydaO motif RNA recently was reported to weakly bind ATP, we report that numerous members of this noncoding RNA class selectively respond to c-di-AMP with subnanomolar affinity. Our findings resolve the mystery regarding the primary ligand for this extremely common riboswitch class and expose a major portion of the super-regulon of genes that are controlled by the widespread bacterial Second Messenger c-di-AMP.
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an allosteric self splicing ribozyme triggered by a bacterial Second Messenger
Science, 2010Co-Authors: Elaine R Lee, Zasha Weinberg, N. Sudarsan, Ronald R Breaker, Jenny L BakerAbstract:Group I self-splicing ribozymes commonly function as components of selfish mobile genetic elements. We identified an allosteric group I ribozyme, wherein self-splicing is regulated by a distinct riboswitch class that senses the bacterial Second Messenger c-di-GMP. The tandem RNA sensory system resides in the 5' untranslated region of the Messenger RNA for a putative virulence gene in the pathogenic bacterium Clostridium difficile. c-di-GMP binding by the riboswitch induces folding changes at atypical splice site junctions to modulate alternative RNA processing. Our findings indicate that some self-splicing ribozymes are not selfish elements but are harnessed by cells as metabolite sensors and genetic regulators.
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Riboswitches in eubacteria sense the Second Messenger cyclic Di-GMP
Science, 2008Co-Authors: N. Sudarsan, E. R. Lee, Zasha Weinberg, R. H. Moy, K. H. Link, Ronald R BreakerAbstract:Cyclic di-guanosine monophosphate (di-GMP) is a circular RNA dinucleotide that functions as a Second Messenger in diverse species of bacteria to trigger wide-ranging physiological changes, including cell differentiation, conversion between motile and biofilm lifestyles, and virulence gene expression. However, the mechanisms by which cyclic di-GMP regulates gene expression have remained a mystery. We found that cyclic di-GMP in many bacterial species is sensed by a riboswitch class in Messenger RNA that controls the expression of genes involved in numerous fundamental cellular processes. A variety of cyclic di-GMP regulons are revealed, including some riboswitches associated with virulence gene expression, pilus formation, and flagellum biosynthesis. In addition, sequences matching the consensus for cyclic di-GMP riboswitches are present in the genome of a bacteriophage.
Kazuhiro Furukawa - One of the best experts on this subject based on the ideXlab platform.
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riboswitches in eubacteria sense the Second Messenger c di amp
Nature Chemical Biology, 2013Co-Authors: James W Nelson, Zasha Weinberg, N. Sudarsan, Kazuhiro Furukawa, Joy Xin Wang, Ronald R BreakerAbstract:Cyclic di-adenosine monophosphate (c-di-AMP) is a recently discovered bacterial Second Messenger implicated in the control of cell wall metabolism, osmotic stress responses and sporulation. However, the mechanisms by which c-di-AMP triggers these physiological responses have remained largely unknown. Notably, a candidate riboswitch class called ydaO associates with numerous genes involved in these same processes. Although a representative ydaO motif RNA recently was reported to weakly bind ATP, we report that numerous members of this noncoding RNA class selectively respond to c-di-AMP with subnanomolar affinity. Our findings resolve the mystery regarding the primary ligand for this extremely common riboswitch class and expose a major portion of the super-regulon of genes that are controlled by the widespread bacterial Second Messenger c-di-AMP.
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riboswitches in eubacteria sense the Second Messenger c di amp
Nature Chemical Biology, 2013Co-Authors: James W Nelson, Zasha Weinberg, N. Sudarsan, Kazuhiro Furukawa, Joy Xin Wang, Ronald R BreakerAbstract:Cyclic diadenosine monophosphate (c-di-AMP) is a newly identified nucleotide Second Messenger in bacteria. Though protein receptors for c-di-AMP are known, the ydaO riboswitch has now been validated as a physiological sensor of cellular c-di-AMP levels.
-
engineered allosteric ribozymes that sense the bacterial Second Messenger cyclic diguanosyl 5 monophosphate
Analytical Chemistry, 2012Co-Authors: Kazuhiro Furukawa, Ronald R BreakerAbstract:A series of allosteric ribozymes that respond to the bacterial Second Messenger cyclic diguanosyl-5′-monophosphate (c-di-GMP) have been created by using in vitro selection. An RNA library was gener...
Joy Xin Wang - One of the best experts on this subject based on the ideXlab platform.
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riboswitches in eubacteria sense the Second Messenger c di amp
Nature Chemical Biology, 2013Co-Authors: James W Nelson, Zasha Weinberg, N. Sudarsan, Kazuhiro Furukawa, Joy Xin Wang, Ronald R BreakerAbstract:Cyclic di-adenosine monophosphate (c-di-AMP) is a recently discovered bacterial Second Messenger implicated in the control of cell wall metabolism, osmotic stress responses and sporulation. However, the mechanisms by which c-di-AMP triggers these physiological responses have remained largely unknown. Notably, a candidate riboswitch class called ydaO associates with numerous genes involved in these same processes. Although a representative ydaO motif RNA recently was reported to weakly bind ATP, we report that numerous members of this noncoding RNA class selectively respond to c-di-AMP with subnanomolar affinity. Our findings resolve the mystery regarding the primary ligand for this extremely common riboswitch class and expose a major portion of the super-regulon of genes that are controlled by the widespread bacterial Second Messenger c-di-AMP.
-
riboswitches in eubacteria sense the Second Messenger c di amp
Nature Chemical Biology, 2013Co-Authors: James W Nelson, Zasha Weinberg, N. Sudarsan, Kazuhiro Furukawa, Joy Xin Wang, Ronald R BreakerAbstract:Cyclic diadenosine monophosphate (c-di-AMP) is a newly identified nucleotide Second Messenger in bacteria. Though protein receptors for c-di-AMP are known, the ydaO riboswitch has now been validated as a physiological sensor of cellular c-di-AMP levels.