The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform
Wojciech Sledz - One of the best experts on this subject based on the ideXlab platform.
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detection identification and differentiation of pectobacterium and dickeya species causing potato blackleg and tuber soft rot a review
Annals of Applied Biology, 2015Co-Authors: Robert Czajkowski, M C M Perombelon, Sylwia Jafra, Ewa Lojkowska, Marta Potrykus, J M Van Der Wolf, Wojciech SledzAbstract:The soft rot Enterobacteriaceae (SRE) Pectobacterium and Dickeya species (formerly classified as pectinolytic Erwinia spp.) cause important diseases on potato and other arable and horticultural crops. They may affect the growing potato plant causing blackleg and are responsible for tuber soft rot in storage thereby reducing yield and quality. Efficient and cost-effective detection and identification methods are essential to investigate the ecology and pathogenesis of the SRE as well as in Seed Certification programmes. The aim of this review was to collect all existing information on methods available for SRE detection. The review reports on the sampling and preparation of plant material for testing and on over thirty methods to detect, identify and differentiate the soft rot and blackleg causing bacteria to species and subspecies level. These include methods based on biochemical characters, serology, molecular techniques which rely on DNA sequence amplification as well as several less-investigated ones.
Robert Czajkowski - One of the best experts on this subject based on the ideXlab platform.
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detection identification and differentiation of pectobacterium and dickeya species causing potato blackleg and tuber soft rot a review
Annals of Applied Biology, 2015Co-Authors: Robert Czajkowski, M C M Perombelon, Sylwia Jafra, Ewa Lojkowska, Marta Potrykus, J M Van Der Wolf, Wojciech SledzAbstract:The soft rot Enterobacteriaceae (SRE) Pectobacterium and Dickeya species (formerly classified as pectinolytic Erwinia spp.) cause important diseases on potato and other arable and horticultural crops. They may affect the growing potato plant causing blackleg and are responsible for tuber soft rot in storage thereby reducing yield and quality. Efficient and cost-effective detection and identification methods are essential to investigate the ecology and pathogenesis of the SRE as well as in Seed Certification programmes. The aim of this review was to collect all existing information on methods available for SRE detection. The review reports on the sampling and preparation of plant material for testing and on over thirty methods to detect, identify and differentiate the soft rot and blackleg causing bacteria to species and subspecies level. These include methods based on biochemical characters, serology, molecular techniques which rely on DNA sequence amplification as well as several less-investigated ones.
Joanne Russell - One of the best experts on this subject based on the ideXlab platform.
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Single nucleotide polymorphism assay to distinguish barley (Hordeum vulgare L.) varieties in support of Seed Certification
Genetic Resources and Crop Evolution, 2019Co-Authors: Heather Owen, Karen Pearson, Adrian M. I. Roberts, Alex Reid, Joanne RussellAbstract:Seed Certification of barley ( Hordeum vulgare L.) involves scoring morphological characteristics to determine the variety in field plots which is expensive and time consuming. In order to establish whether molecular tests can identify the barley variety we have developed a molecular test to be used on Seed. The molecular test genotypes the Seed samples using 38 Single Nucleotide Polymorphisms (SNPs) and can distinguish the varieties that are currently in commercial production in Scotland. The 38 SNP set has the ability to distinguish most of the 700+ contemporary barley cultivars that have previously been genotyped. This SNP test was developed to support the current Seed Certification system at Science and Advice for Scottish Agriculture (SASA), Scottish Government. SASA certifies all of the barley Seed produced in Scotland according to EU regulations.
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single nucleotide polymorphism assay to distinguish barley hordeum vulgare l varieties in support of Seed Certification
Genetic Resources and Crop Evolution, 2019Co-Authors: Heather Owen, Karen Pearson, Adrian M. I. Roberts, Alex Reid, Joanne RussellAbstract:Seed Certification of barley (Hordeum vulgare L.) involves scoring morphological characteristics to determine the variety in field plots which is expensive and time consuming. In order to establish whether molecular tests can identify the barley variety we have developed a molecular test to be used on Seed. The molecular test genotypes the Seed samples using 38 Single Nucleotide Polymorphisms (SNPs) and can distinguish the varieties that are currently in commercial production in Scotland. The 38 SNP set has the ability to distinguish most of the 700+ contemporary barley cultivars that have previously been genotyped. This SNP test was developed to support the current Seed Certification system at Science and Advice for Scottish Agriculture (SASA), Scottish Government. SASA certifies all of the barley Seed produced in Scotland according to EU regulations.
Huimin Xu - One of the best experts on this subject based on the ideXlab platform.
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Potato virus Y: An evolving concern for potato crops in the United States and Canada
Plant Disease, 2010Co-Authors: Stewart Gray, Johan Lorenzen, Solke De Boer, Phillip Nolte, Rudra Singh, Jonathan Whitworth, Alexander Karasev, Alain Boucher, Huimin XuAbstract:North American potato production differs from other geographical regions such as Europe in that it is essentially a closed system, i.e., Seed potatoes are not imported and production is dominated by only a few cultivars. The lack of significant Seed imports provides a mechanism for Seed Certification to be extremely effective at minimizing virus levels in Seed lots, especially if the changes in Seed laws, postharvest testing, and tolerance limits discussed above are adopted. This is an opportunity to effectively manage PVY at levels that are at or below detection and well below economic significance. Aiding the Seed Certification programs in the adoption of the Canada/US-Management Plan for Potato Viruses that Cause Tuber Necrosis has and continues to build consensus and cooperation within the industry to reform and modernize Seed Certification practices and, as importantly, modernize best management practices that growers can implement so that their production meets or exceeds virus tolerances set within the Seed Certification standards. Seed inspectors could also benefit from continually updated information from the research community to help them better recognize the spectrum of symptoms caused by the various strains and variants of PVY in all the different cultivars now being grown in their states and provinces. They could also benefit from improved field diagnostics that will assist them and the growers in identifying problem plants that should be rogued. If PVY levels in Seed can be minimized and on-farm management strategies can be optimized, then PVY incidence in the potato crop will be marginalized. The restricted distribution of the tuber necrotic strains also offers an opportunity to prevent these strains from becoming economically significant if appropriate testing of Seed lots in those areas could prevent them from being planted. Shipping point inspections of tubers will also help in identifying and eliminating tuber necrotic viruses. The dominance of a few cultivars has been eroding in recent years. Russet Burbank, a cultivar introduced over 100 years ago, still accounts for 40 to 50% of the U.S. acreage, but acreage in the Northwestern United States has been declining steadily as other russet cultivars come on the market and gain acceptance. Potato cultivar has had a significant impact on the PVY problem, as with the release and widespread acceptance of Shepody, Russet Norkotah, and other asymptomatic carriers of PVY (http://oregonstate. edu/potatoes/latenttoPVYlist.htm), which in 2008 comprised more than 15 and 12% of the total U.S. and Canadian Seed acreage, respectively. These cultivars have certainly contributed to the overall increase in PVY in the Seed potato crop and by extension the commercial potato crop. The increased diversity of potato cultivars grown in both countries has also introduced a wider spectrum of PVY symptoms, most notably the milder symptoms that are characteristic of the PVYN/NTN and PVYN-Wi strains on many cultivars. Since the success of Seed Certification is dependent upon visual assessment of the crop, mild or absent symptoms means that many more infected plants go unnoticed. The more symptomatic PVYO strains are observed and removed, but the other strains remain in the crop and are passed along in the Seed, contributing to an overall increase in PVY incidence and more importantly to a shift in PVY strain composition. The U.S. and Canadian potato industry stakeholders are increasingly aware of the PVY-associated challenges and have been moving rapidly to work with researchers and all aspects of the industry to implement plans to suppress PVY incidence. Continued education of growers, Seed Certification officials, and researchers alike, coupled with the development and adoption of new or revised best management practices and diagnostic tools, and the renewed inter est of breeders to develop virus resistant cultivars, will be the keys to success in bringing PVY incidence under control and in minimizing tuber necrotic strains.
Marta Potrykus - One of the best experts on this subject based on the ideXlab platform.
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detection identification and differentiation of pectobacterium and dickeya species causing potato blackleg and tuber soft rot a review
Annals of Applied Biology, 2015Co-Authors: Robert Czajkowski, M C M Perombelon, Sylwia Jafra, Ewa Lojkowska, Marta Potrykus, J M Van Der Wolf, Wojciech SledzAbstract:The soft rot Enterobacteriaceae (SRE) Pectobacterium and Dickeya species (formerly classified as pectinolytic Erwinia spp.) cause important diseases on potato and other arable and horticultural crops. They may affect the growing potato plant causing blackleg and are responsible for tuber soft rot in storage thereby reducing yield and quality. Efficient and cost-effective detection and identification methods are essential to investigate the ecology and pathogenesis of the SRE as well as in Seed Certification programmes. The aim of this review was to collect all existing information on methods available for SRE detection. The review reports on the sampling and preparation of plant material for testing and on over thirty methods to detect, identify and differentiate the soft rot and blackleg causing bacteria to species and subspecies level. These include methods based on biochemical characters, serology, molecular techniques which rely on DNA sequence amplification as well as several less-investigated ones.
