The Experts below are selected from a list of 107988 Experts worldwide ranked by ideXlab platform
Jian Zhang - One of the best experts on this subject based on the ideXlab platform.
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A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.).
International journal of molecular sciences, 2017Co-Authors: Yifeng Wang, Juan Zhao, Xiaohong Tong, Yuxuan Hou, Jiehua Qiu, Jian ZhangAbstract:PKA (protein lysine acetylation) is a critical post-translational modification that regulates various Developmental processes, including Seed Development. However, the acetylation events and dynamics on a proteomic scale in this process remain largely unknown, especially in rice early Seed Development. We report the first quantitative acetylproteomic study focused on rice early Seed Development by employing a mass spectral-based (MS-based), label-free approach. A total of 1817 acetylsites on 1688 acetylpeptides from 972 acetylproteins were identified in pistils and Seeds at three and seven days after pollination, including 268 acetyproteins differentially acetylated among the three stages. Motif-X analysis revealed that six significantly enriched motifs, such as (DxkK), (kH) and (kY) around the acetylsites of the identified rice Seed acetylproteins. Differentially acetylated proteins among the three stages, including adenosine diphosphate (ADP) -glucose pyrophosphorylases (AGPs), PDIL1-1 (protein disulfide isomerase like 1-1), hexokinases, pyruvate dehydrogenase complex (PDC) and numerous other regulators that are extensively involved in the starch and sucrose metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle and photosynthesis pathways during early Seed Development. This study greatly expanded the rice acetylome dataset, and shed novel insight into the regulatory roles of PKA in rice early Seed Development.
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Quantitative phosphoproteomic analysis of early Seed Development in rice (Oryza sativa L.)
Plant molecular biology, 2015Co-Authors: Jiehua Qiu, Yifeng Wang, Xiaohong Tong, Yuxuan Hou, Haiyan Lin, Qing Liu, Wen Zhang, Babi Ramesh Reddy Nallamilli, Jian ZhangAbstract:Rice (Oryza sativa L.) Seed serves as a major food source for over half of the global population. Though it has been long recognized that phosphorylation plays an essential role in rice Seed Development, the phosphorylation events and dynamics in this process remain largely unknown so far. Here, we report the first large scale identification of rice Seed phosphoproteins and phosphosites by using a quantitative phosphoproteomic approach. Thorough proteomic studies in pistils and Seeds at 3, 7 days after pollination resulted in the successful identification of 3885, 4313 and 4135 phosphopeptides respectively. A total of 2487 proteins were differentially phosphorylated among the three stages, including Kip related protein 1, Rice basic leucine zipper factor 1, Rice prolamin box binding factor and numerous other master regulators of rice Seed Development. Moreover, differentially phosphorylated proteins may be extensively involved in the biosynthesis and signaling pathways of phytohormones such as auxin, gibberellin, abscisic acid and brassinosteroid. Our results strongly indicated that protein phosphorylation is a key mechanism regulating cell proliferation and enlargement, phytohormone biosynthesis and signaling, grain filling and grain quality during rice Seed Development. Overall, the current study enhanced our understanding of the rice phosphoproteome and shed novel insight into the regulatory mechanism of rice Seed Development.
Yifeng Wang - One of the best experts on this subject based on the ideXlab platform.
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Genome-Wide Identification of lncRNAs During Rice Seed Development
Genes, 2020Co-Authors: Juan Zhao, Abolore Adijat Ajadi, Yifeng Wang, Xiaohong Tong, Huimei Wang, Liqun Tang, Yazhou Shu, Xixi LiuAbstract:Rice Seed is a pivotal reproductive organ that directly determines yield and quality. Long non-coding RNAs (lncRNAs) have been recognized as key regulators in plant Development, but the roles of lncRNAs in rice Seed Development remain unclear. In this study, we performed a paired-end RNA sequencing in samples of rice pistils and Seeds at three and seven days after pollination (DAP) respectively. A total of 540 lncRNAs were obtained, among which 482 lncRNAs had significantly different expression patterns during Seed Development. Results from semi-qPCR conducted on 15 randomly selected differentially expressed lncRNAs suggested high reliability of the transcriptomic data. RNA interference of TCONS_00023703, which is predominantly transcribed in developing Seeds, significantly reduced grain length and thousand-grain weight. These results expanded the dataset of lncRNA in rice and enhanced our understanding of the biological functions of lncRNAs in rice Seed Development
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A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.).
International journal of molecular sciences, 2017Co-Authors: Yifeng Wang, Juan Zhao, Xiaohong Tong, Yuxuan Hou, Jiehua Qiu, Jian ZhangAbstract:PKA (protein lysine acetylation) is a critical post-translational modification that regulates various Developmental processes, including Seed Development. However, the acetylation events and dynamics on a proteomic scale in this process remain largely unknown, especially in rice early Seed Development. We report the first quantitative acetylproteomic study focused on rice early Seed Development by employing a mass spectral-based (MS-based), label-free approach. A total of 1817 acetylsites on 1688 acetylpeptides from 972 acetylproteins were identified in pistils and Seeds at three and seven days after pollination, including 268 acetyproteins differentially acetylated among the three stages. Motif-X analysis revealed that six significantly enriched motifs, such as (DxkK), (kH) and (kY) around the acetylsites of the identified rice Seed acetylproteins. Differentially acetylated proteins among the three stages, including adenosine diphosphate (ADP) -glucose pyrophosphorylases (AGPs), PDIL1-1 (protein disulfide isomerase like 1-1), hexokinases, pyruvate dehydrogenase complex (PDC) and numerous other regulators that are extensively involved in the starch and sucrose metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle and photosynthesis pathways during early Seed Development. This study greatly expanded the rice acetylome dataset, and shed novel insight into the regulatory roles of PKA in rice early Seed Development.
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Quantitative phosphoproteomic analysis of early Seed Development in rice (Oryza sativa L.)
Plant molecular biology, 2015Co-Authors: Jiehua Qiu, Yifeng Wang, Xiaohong Tong, Yuxuan Hou, Haiyan Lin, Qing Liu, Wen Zhang, Babi Ramesh Reddy Nallamilli, Jian ZhangAbstract:Rice (Oryza sativa L.) Seed serves as a major food source for over half of the global population. Though it has been long recognized that phosphorylation plays an essential role in rice Seed Development, the phosphorylation events and dynamics in this process remain largely unknown so far. Here, we report the first large scale identification of rice Seed phosphoproteins and phosphosites by using a quantitative phosphoproteomic approach. Thorough proteomic studies in pistils and Seeds at 3, 7 days after pollination resulted in the successful identification of 3885, 4313 and 4135 phosphopeptides respectively. A total of 2487 proteins were differentially phosphorylated among the three stages, including Kip related protein 1, Rice basic leucine zipper factor 1, Rice prolamin box binding factor and numerous other master regulators of rice Seed Development. Moreover, differentially phosphorylated proteins may be extensively involved in the biosynthesis and signaling pathways of phytohormones such as auxin, gibberellin, abscisic acid and brassinosteroid. Our results strongly indicated that protein phosphorylation is a key mechanism regulating cell proliferation and enlargement, phytohormone biosynthesis and signaling, grain filling and grain quality during rice Seed Development. Overall, the current study enhanced our understanding of the rice phosphoproteome and shed novel insight into the regulatory mechanism of rice Seed Development.
Jiehua Qiu - One of the best experts on this subject based on the ideXlab platform.
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A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.).
International journal of molecular sciences, 2017Co-Authors: Yifeng Wang, Juan Zhao, Xiaohong Tong, Yuxuan Hou, Jiehua Qiu, Jian ZhangAbstract:PKA (protein lysine acetylation) is a critical post-translational modification that regulates various Developmental processes, including Seed Development. However, the acetylation events and dynamics on a proteomic scale in this process remain largely unknown, especially in rice early Seed Development. We report the first quantitative acetylproteomic study focused on rice early Seed Development by employing a mass spectral-based (MS-based), label-free approach. A total of 1817 acetylsites on 1688 acetylpeptides from 972 acetylproteins were identified in pistils and Seeds at three and seven days after pollination, including 268 acetyproteins differentially acetylated among the three stages. Motif-X analysis revealed that six significantly enriched motifs, such as (DxkK), (kH) and (kY) around the acetylsites of the identified rice Seed acetylproteins. Differentially acetylated proteins among the three stages, including adenosine diphosphate (ADP) -glucose pyrophosphorylases (AGPs), PDIL1-1 (protein disulfide isomerase like 1-1), hexokinases, pyruvate dehydrogenase complex (PDC) and numerous other regulators that are extensively involved in the starch and sucrose metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle and photosynthesis pathways during early Seed Development. This study greatly expanded the rice acetylome dataset, and shed novel insight into the regulatory roles of PKA in rice early Seed Development.
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Quantitative phosphoproteomic analysis of early Seed Development in rice (Oryza sativa L.)
Plant molecular biology, 2015Co-Authors: Jiehua Qiu, Yifeng Wang, Xiaohong Tong, Yuxuan Hou, Haiyan Lin, Qing Liu, Wen Zhang, Babi Ramesh Reddy Nallamilli, Jian ZhangAbstract:Rice (Oryza sativa L.) Seed serves as a major food source for over half of the global population. Though it has been long recognized that phosphorylation plays an essential role in rice Seed Development, the phosphorylation events and dynamics in this process remain largely unknown so far. Here, we report the first large scale identification of rice Seed phosphoproteins and phosphosites by using a quantitative phosphoproteomic approach. Thorough proteomic studies in pistils and Seeds at 3, 7 days after pollination resulted in the successful identification of 3885, 4313 and 4135 phosphopeptides respectively. A total of 2487 proteins were differentially phosphorylated among the three stages, including Kip related protein 1, Rice basic leucine zipper factor 1, Rice prolamin box binding factor and numerous other master regulators of rice Seed Development. Moreover, differentially phosphorylated proteins may be extensively involved in the biosynthesis and signaling pathways of phytohormones such as auxin, gibberellin, abscisic acid and brassinosteroid. Our results strongly indicated that protein phosphorylation is a key mechanism regulating cell proliferation and enlargement, phytohormone biosynthesis and signaling, grain filling and grain quality during rice Seed Development. Overall, the current study enhanced our understanding of the rice phosphoproteome and shed novel insight into the regulatory mechanism of rice Seed Development.
Xiaohong Tong - One of the best experts on this subject based on the ideXlab platform.
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Genome-Wide Identification of lncRNAs During Rice Seed Development
Genes, 2020Co-Authors: Juan Zhao, Abolore Adijat Ajadi, Yifeng Wang, Xiaohong Tong, Huimei Wang, Liqun Tang, Yazhou Shu, Xixi LiuAbstract:Rice Seed is a pivotal reproductive organ that directly determines yield and quality. Long non-coding RNAs (lncRNAs) have been recognized as key regulators in plant Development, but the roles of lncRNAs in rice Seed Development remain unclear. In this study, we performed a paired-end RNA sequencing in samples of rice pistils and Seeds at three and seven days after pollination (DAP) respectively. A total of 540 lncRNAs were obtained, among which 482 lncRNAs had significantly different expression patterns during Seed Development. Results from semi-qPCR conducted on 15 randomly selected differentially expressed lncRNAs suggested high reliability of the transcriptomic data. RNA interference of TCONS_00023703, which is predominantly transcribed in developing Seeds, significantly reduced grain length and thousand-grain weight. These results expanded the dataset of lncRNA in rice and enhanced our understanding of the biological functions of lncRNAs in rice Seed Development
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A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.).
International journal of molecular sciences, 2017Co-Authors: Yifeng Wang, Juan Zhao, Xiaohong Tong, Yuxuan Hou, Jiehua Qiu, Jian ZhangAbstract:PKA (protein lysine acetylation) is a critical post-translational modification that regulates various Developmental processes, including Seed Development. However, the acetylation events and dynamics on a proteomic scale in this process remain largely unknown, especially in rice early Seed Development. We report the first quantitative acetylproteomic study focused on rice early Seed Development by employing a mass spectral-based (MS-based), label-free approach. A total of 1817 acetylsites on 1688 acetylpeptides from 972 acetylproteins were identified in pistils and Seeds at three and seven days after pollination, including 268 acetyproteins differentially acetylated among the three stages. Motif-X analysis revealed that six significantly enriched motifs, such as (DxkK), (kH) and (kY) around the acetylsites of the identified rice Seed acetylproteins. Differentially acetylated proteins among the three stages, including adenosine diphosphate (ADP) -glucose pyrophosphorylases (AGPs), PDIL1-1 (protein disulfide isomerase like 1-1), hexokinases, pyruvate dehydrogenase complex (PDC) and numerous other regulators that are extensively involved in the starch and sucrose metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle and photosynthesis pathways during early Seed Development. This study greatly expanded the rice acetylome dataset, and shed novel insight into the regulatory roles of PKA in rice early Seed Development.
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Quantitative phosphoproteomic analysis of early Seed Development in rice (Oryza sativa L.)
Plant molecular biology, 2015Co-Authors: Jiehua Qiu, Yifeng Wang, Xiaohong Tong, Yuxuan Hou, Haiyan Lin, Qing Liu, Wen Zhang, Babi Ramesh Reddy Nallamilli, Jian ZhangAbstract:Rice (Oryza sativa L.) Seed serves as a major food source for over half of the global population. Though it has been long recognized that phosphorylation plays an essential role in rice Seed Development, the phosphorylation events and dynamics in this process remain largely unknown so far. Here, we report the first large scale identification of rice Seed phosphoproteins and phosphosites by using a quantitative phosphoproteomic approach. Thorough proteomic studies in pistils and Seeds at 3, 7 days after pollination resulted in the successful identification of 3885, 4313 and 4135 phosphopeptides respectively. A total of 2487 proteins were differentially phosphorylated among the three stages, including Kip related protein 1, Rice basic leucine zipper factor 1, Rice prolamin box binding factor and numerous other master regulators of rice Seed Development. Moreover, differentially phosphorylated proteins may be extensively involved in the biosynthesis and signaling pathways of phytohormones such as auxin, gibberellin, abscisic acid and brassinosteroid. Our results strongly indicated that protein phosphorylation is a key mechanism regulating cell proliferation and enlargement, phytohormone biosynthesis and signaling, grain filling and grain quality during rice Seed Development. Overall, the current study enhanced our understanding of the rice phosphoproteome and shed novel insight into the regulatory mechanism of rice Seed Development.
Yuxuan Hou - One of the best experts on this subject based on the ideXlab platform.
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A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.).
International journal of molecular sciences, 2017Co-Authors: Yifeng Wang, Juan Zhao, Xiaohong Tong, Yuxuan Hou, Jiehua Qiu, Jian ZhangAbstract:PKA (protein lysine acetylation) is a critical post-translational modification that regulates various Developmental processes, including Seed Development. However, the acetylation events and dynamics on a proteomic scale in this process remain largely unknown, especially in rice early Seed Development. We report the first quantitative acetylproteomic study focused on rice early Seed Development by employing a mass spectral-based (MS-based), label-free approach. A total of 1817 acetylsites on 1688 acetylpeptides from 972 acetylproteins were identified in pistils and Seeds at three and seven days after pollination, including 268 acetyproteins differentially acetylated among the three stages. Motif-X analysis revealed that six significantly enriched motifs, such as (DxkK), (kH) and (kY) around the acetylsites of the identified rice Seed acetylproteins. Differentially acetylated proteins among the three stages, including adenosine diphosphate (ADP) -glucose pyrophosphorylases (AGPs), PDIL1-1 (protein disulfide isomerase like 1-1), hexokinases, pyruvate dehydrogenase complex (PDC) and numerous other regulators that are extensively involved in the starch and sucrose metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle and photosynthesis pathways during early Seed Development. This study greatly expanded the rice acetylome dataset, and shed novel insight into the regulatory roles of PKA in rice early Seed Development.
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Quantitative phosphoproteomic analysis of early Seed Development in rice (Oryza sativa L.)
Plant molecular biology, 2015Co-Authors: Jiehua Qiu, Yifeng Wang, Xiaohong Tong, Yuxuan Hou, Haiyan Lin, Qing Liu, Wen Zhang, Babi Ramesh Reddy Nallamilli, Jian ZhangAbstract:Rice (Oryza sativa L.) Seed serves as a major food source for over half of the global population. Though it has been long recognized that phosphorylation plays an essential role in rice Seed Development, the phosphorylation events and dynamics in this process remain largely unknown so far. Here, we report the first large scale identification of rice Seed phosphoproteins and phosphosites by using a quantitative phosphoproteomic approach. Thorough proteomic studies in pistils and Seeds at 3, 7 days after pollination resulted in the successful identification of 3885, 4313 and 4135 phosphopeptides respectively. A total of 2487 proteins were differentially phosphorylated among the three stages, including Kip related protein 1, Rice basic leucine zipper factor 1, Rice prolamin box binding factor and numerous other master regulators of rice Seed Development. Moreover, differentially phosphorylated proteins may be extensively involved in the biosynthesis and signaling pathways of phytohormones such as auxin, gibberellin, abscisic acid and brassinosteroid. Our results strongly indicated that protein phosphorylation is a key mechanism regulating cell proliferation and enlargement, phytohormone biosynthesis and signaling, grain filling and grain quality during rice Seed Development. Overall, the current study enhanced our understanding of the rice phosphoproteome and shed novel insight into the regulatory mechanism of rice Seed Development.
