The Experts below are selected from a list of 252 Experts worldwide ranked by ideXlab platform
David J. Gifford - One of the best experts on this subject based on the ideXlab platform.
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changes in mrna populations during loblolly pine pinus taeda Seed Stratification germination and post germinative growth
Physiologia Plantarum, 1996Co-Authors: Robert T. Mullen, Janice E. King, David J. GiffordAbstract:Changes in the pattems of gene expression were examined during loblolly pine (Pinus taeda L.) Seed Stratification, germination, and post-germinative growth. In both the megagametophyte and the embryo, DNA contents remained relatively constant at all stages examined. RNA contents, however, increased in both tissues following Seed germination, particularly in the embryo where a 7-fold increase in the RNA content was observed 5 days after germination. Poly(A) + RNA, extracted from megagametophytes and embryos, was translated in vitro in a rabbit reticulocyte lysate cell-free system. Analysis of [ 35 S]-methionine-labelled translation products by two-dimensional electrophoresis/fluorography indicated that there were changes in the populations of mRNAs during all developmental stages examined. In both the megagametophyte and the embryo several distinct mRNA populations, including one constitutively present at all stages examined, were identified. One mRNA population, present in the mature Seed, decreased during Seed Stratification. Another population, not present in the mature Seed, rose during the period of Stratification that coincided with an increase in Seed germinability. A third population, which appeared during Seed germination, increased steadily during post-germinative growth. Besides these similarities, specific differences between megagametophyte and embryo were noted. For example, one mRNA population, which was present in the megagametophyte of the mature Seed and remained constant during the Stratification period, disappeared immediately following Seed germination. In the embryo, one set of messages was germination specific. In total, these results show that mRNA populations change in a temporal fashion that is consistent with the patterns of de novo protein synthesis known to occur in loblolly pine during the same developmental periods.
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Changes in mRNA populations during loblolly pine (Pinus taeda) Seed Stratification, germination and post‐germinative growth
Physiologia Plantarum, 1996Co-Authors: Robert T. Mullen, Janice E. King, David J. GiffordAbstract:Changes in the pattems of gene expression were examined during loblolly pine (Pinus taeda L.) Seed Stratification, germination, and post-germinative growth. In both the megagametophyte and the embryo, DNA contents remained relatively constant at all stages examined. RNA contents, however, increased in both tissues following Seed germination, particularly in the embryo where a 7-fold increase in the RNA content was observed 5 days after germination. Poly(A) + RNA, extracted from megagametophytes and embryos, was translated in vitro in a rabbit reticulocyte lysate cell-free system. Analysis of [ 35 S]-methionine-labelled translation products by two-dimensional electrophoresis/fluorography indicated that there were changes in the populations of mRNAs during all developmental stages examined. In both the megagametophyte and the embryo several distinct mRNA populations, including one constitutively present at all stages examined, were identified. One mRNA population, present in the mature Seed, decreased during Seed Stratification. Another population, not present in the mature Seed, rose during the period of Stratification that coincided with an increase in Seed germinability. A third population, which appeared during Seed germination, increased steadily during post-germinative growth. Besides these similarities, specific differences between megagametophyte and embryo were noted. For example, one mRNA population, which was present in the megagametophyte of the mature Seed and remained constant during the Stratification period, disappeared immediately following Seed germination. In the embryo, one set of messages was germination specific. In total, these results show that mRNA populations change in a temporal fashion that is consistent with the patterns of de novo protein synthesis known to occur in loblolly pine during the same developmental periods.
Robert T. Mullen - One of the best experts on this subject based on the ideXlab platform.
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changes in mrna populations during loblolly pine pinus taeda Seed Stratification germination and post germinative growth
Physiologia Plantarum, 1996Co-Authors: Robert T. Mullen, Janice E. King, David J. GiffordAbstract:Changes in the pattems of gene expression were examined during loblolly pine (Pinus taeda L.) Seed Stratification, germination, and post-germinative growth. In both the megagametophyte and the embryo, DNA contents remained relatively constant at all stages examined. RNA contents, however, increased in both tissues following Seed germination, particularly in the embryo where a 7-fold increase in the RNA content was observed 5 days after germination. Poly(A) + RNA, extracted from megagametophytes and embryos, was translated in vitro in a rabbit reticulocyte lysate cell-free system. Analysis of [ 35 S]-methionine-labelled translation products by two-dimensional electrophoresis/fluorography indicated that there were changes in the populations of mRNAs during all developmental stages examined. In both the megagametophyte and the embryo several distinct mRNA populations, including one constitutively present at all stages examined, were identified. One mRNA population, present in the mature Seed, decreased during Seed Stratification. Another population, not present in the mature Seed, rose during the period of Stratification that coincided with an increase in Seed germinability. A third population, which appeared during Seed germination, increased steadily during post-germinative growth. Besides these similarities, specific differences between megagametophyte and embryo were noted. For example, one mRNA population, which was present in the megagametophyte of the mature Seed and remained constant during the Stratification period, disappeared immediately following Seed germination. In the embryo, one set of messages was germination specific. In total, these results show that mRNA populations change in a temporal fashion that is consistent with the patterns of de novo protein synthesis known to occur in loblolly pine during the same developmental periods.
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Changes in mRNA populations during loblolly pine (Pinus taeda) Seed Stratification, germination and post‐germinative growth
Physiologia Plantarum, 1996Co-Authors: Robert T. Mullen, Janice E. King, David J. GiffordAbstract:Changes in the pattems of gene expression were examined during loblolly pine (Pinus taeda L.) Seed Stratification, germination, and post-germinative growth. In both the megagametophyte and the embryo, DNA contents remained relatively constant at all stages examined. RNA contents, however, increased in both tissues following Seed germination, particularly in the embryo where a 7-fold increase in the RNA content was observed 5 days after germination. Poly(A) + RNA, extracted from megagametophytes and embryos, was translated in vitro in a rabbit reticulocyte lysate cell-free system. Analysis of [ 35 S]-methionine-labelled translation products by two-dimensional electrophoresis/fluorography indicated that there were changes in the populations of mRNAs during all developmental stages examined. In both the megagametophyte and the embryo several distinct mRNA populations, including one constitutively present at all stages examined, were identified. One mRNA population, present in the mature Seed, decreased during Seed Stratification. Another population, not present in the mature Seed, rose during the period of Stratification that coincided with an increase in Seed germinability. A third population, which appeared during Seed germination, increased steadily during post-germinative growth. Besides these similarities, specific differences between megagametophyte and embryo were noted. For example, one mRNA population, which was present in the megagametophyte of the mature Seed and remained constant during the Stratification period, disappeared immediately following Seed germination. In the embryo, one set of messages was germination specific. In total, these results show that mRNA populations change in a temporal fashion that is consistent with the patterns of de novo protein synthesis known to occur in loblolly pine during the same developmental periods.
Janice E. King - One of the best experts on this subject based on the ideXlab platform.
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changes in mrna populations during loblolly pine pinus taeda Seed Stratification germination and post germinative growth
Physiologia Plantarum, 1996Co-Authors: Robert T. Mullen, Janice E. King, David J. GiffordAbstract:Changes in the pattems of gene expression were examined during loblolly pine (Pinus taeda L.) Seed Stratification, germination, and post-germinative growth. In both the megagametophyte and the embryo, DNA contents remained relatively constant at all stages examined. RNA contents, however, increased in both tissues following Seed germination, particularly in the embryo where a 7-fold increase in the RNA content was observed 5 days after germination. Poly(A) + RNA, extracted from megagametophytes and embryos, was translated in vitro in a rabbit reticulocyte lysate cell-free system. Analysis of [ 35 S]-methionine-labelled translation products by two-dimensional electrophoresis/fluorography indicated that there were changes in the populations of mRNAs during all developmental stages examined. In both the megagametophyte and the embryo several distinct mRNA populations, including one constitutively present at all stages examined, were identified. One mRNA population, present in the mature Seed, decreased during Seed Stratification. Another population, not present in the mature Seed, rose during the period of Stratification that coincided with an increase in Seed germinability. A third population, which appeared during Seed germination, increased steadily during post-germinative growth. Besides these similarities, specific differences between megagametophyte and embryo were noted. For example, one mRNA population, which was present in the megagametophyte of the mature Seed and remained constant during the Stratification period, disappeared immediately following Seed germination. In the embryo, one set of messages was germination specific. In total, these results show that mRNA populations change in a temporal fashion that is consistent with the patterns of de novo protein synthesis known to occur in loblolly pine during the same developmental periods.
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Changes in mRNA populations during loblolly pine (Pinus taeda) Seed Stratification, germination and post‐germinative growth
Physiologia Plantarum, 1996Co-Authors: Robert T. Mullen, Janice E. King, David J. GiffordAbstract:Changes in the pattems of gene expression were examined during loblolly pine (Pinus taeda L.) Seed Stratification, germination, and post-germinative growth. In both the megagametophyte and the embryo, DNA contents remained relatively constant at all stages examined. RNA contents, however, increased in both tissues following Seed germination, particularly in the embryo where a 7-fold increase in the RNA content was observed 5 days after germination. Poly(A) + RNA, extracted from megagametophytes and embryos, was translated in vitro in a rabbit reticulocyte lysate cell-free system. Analysis of [ 35 S]-methionine-labelled translation products by two-dimensional electrophoresis/fluorography indicated that there were changes in the populations of mRNAs during all developmental stages examined. In both the megagametophyte and the embryo several distinct mRNA populations, including one constitutively present at all stages examined, were identified. One mRNA population, present in the mature Seed, decreased during Seed Stratification. Another population, not present in the mature Seed, rose during the period of Stratification that coincided with an increase in Seed germinability. A third population, which appeared during Seed germination, increased steadily during post-germinative growth. Besides these similarities, specific differences between megagametophyte and embryo were noted. For example, one mRNA population, which was present in the megagametophyte of the mature Seed and remained constant during the Stratification period, disappeared immediately following Seed germination. In the embryo, one set of messages was germination specific. In total, these results show that mRNA populations change in a temporal fashion that is consistent with the patterns of de novo protein synthesis known to occur in loblolly pine during the same developmental periods.
Dong Sheng Gao - One of the best experts on this subject based on the ideXlab platform.
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Roles of endoplasmic reticulum stress and unfolded protein response associated genes in Seed Stratification and bud endodormancy during chilling accumulation in Prunus persica.
PloS one, 2014Co-Authors: Xiao Wei, Dong Ling Wang, Min Chen, Qiu Ping Tan, Xiu De Chen, Dong Sheng GaoAbstract:Dormancy mechanisms in Seeds and buds arrest growth until environmental conditions are optimal for development. A genotype-specific period of chilling is usually required to release dormancy, but the underlying molecular mechanisms are still not fully understood. To discover transcriptional pathways associated with dormancy release common to Seed Stratification and bud endodormancy, we explored the chilling-dependent expression of 11 genes involved in endoplasmic reticulum stress and the unfolded protein response signal pathways. We propose that endoplasmic reticulum stress and the unfolded protein response impact on Seed as well as bud germination and development by chilling-dependent mechanisms. The emerging discovery of similarities between Seed Stratification and bud endodormancy status indicate that these two processes are probably regulated by common endoplasmic reticulum stress and unfolded protein response signalling pathways. Clarification of regulatory pathways common to both Seed and bud dormancy may enhance understanding of the mechanisms underlying dormancy and breeding programs may benefit from earlier prediction of chilling requirements for uniform blooming of novel genotypes of deciduous fruit tree species.
Guang-lin Liu - One of the best experts on this subject based on the ideXlab platform.
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Variation in carbohydrates and screening of related differential proteins during the Seed germination of Magnolia sieboldii K. Koch
Trees, 2016Co-Authors: Mei Mei, Xiao-lin Zhang, Guang-lin Liu, Xiao-mei SunAbstract:This paper focuses on carbohydrate metabolism of Magnolia sieboldii during Seed germination by proteomics. 10 differentially accumulated proteins were found. Glycolysis is the most important metabolic pathways. Magnolia sieboldii K. Koch was selected as our research object. M. sieboldii Seeds were initially soaked in clean water for 72 h and then stratified in wet sand with a Seed-to-sand ratio of 1:3 at a fluctuating temperature. The relationship between carbohydrate metabolism and Seed germination, and the localization of related proteins in metabolic pathways were investigated through Seed embryo development, Seed anatomical structure, carbohydrate change, and differentially accumulated protein analysis during Seed Stratification. On the basis of the results, we can divide Seed dormancy and germination into three stages: unchanging embryo length (phase I), slow stretching phase (phase II), and rapid elongation phase (phase III). Two-dimensional electrophoresis revealed that 67 differentially accumulated proteins were obtained in four Seed protein samples corresponding to the four key stages during the Stratification of M. sieboldii Seeds (0, 45, 90, and 110 days of Stratification). Of the 67 differentially accumulated proteins, 7 were related to carbon metabolism and localized in 10 metabolic pathways and 4 were located in the glycolysis/gluconeogenesis pathway and upregulated in the early stage of Seed germination, as indicated by KEGG metabolic pathway analysis. Thus, energy for Seed germination was provided by glycolysis/gluconeogenesis in this stage.
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Proteomic analysis of Magnolia sieboldii K. Koch Seed germination.
Journal of proteomics, 2015Co-Authors: Xiao-lin Zhang, Mei Mei, Guang-lin LiuAbstract:Abstract Magnolia sieboldii is a deciduous tree native to China. This species has a deep dormancy characteristic. To better understand Seed germination, we used protein analysis of changes in Seed protein at 0, 65, 110 and 150 d of Stratification. Comparative 2DE analysis of M. sieboldii Seed protein profiles at 0, 65, 110 and 150 d of Stratification revealed 80 differentially abundance protein species. Comparative analysis showed that ADP-glucose pyrophosphorylase small subunit was degraded during germination. In particular, it was degraded almost completely at 110 d of germination. Starch granules in the microstructure decreased after 65 d of Stratification. Starch granules provided a sufficient amount of substrates and ATPs for subsequent germination. Four storage protein species were identified, of which all were down accumulated. Spots 44 and 46 had different MW and pI values, spots 36 and 46 had nearly the same MW with pI shift in the 2-DE gels, suggesting that they might be present as different isoforms of the same protein family and the post translational modification. Our results suggested that degradation of starch granules and storage protein species prepared the Seed embryo for growth, as well as regulated Seed germination. The present proteomics analysis provides novel insights into the mobilisation of nutrient reserves during the germination of M. sieboldii Seeds. Biological significance To better understand Seed germination, a complex developmental process, we developed a proteome analysis of M. sieboldii Seed. We performed the first comprehensive proteomic and microstructure analysis during different Seed Stratification stages of M. sieboldii. Among the 80 protein species, 26 were identified, 7 and 14 protein species were up or down accumulated significantly. Many of the identified key proteins were involved in embryo development, starch biosynthesis and energy metabolism, Microstructure of Stratification Seed analysis revealed degradation of starch was used for preparing the Seed embryo for growth. These data may help us to develop a comprehensive understanding of the physiological status and mobilisation mechanisms in M. sieboldii Seed germination.