The Experts below are selected from a list of 4902 Experts worldwide ranked by ideXlab platform
Andreas Weihe - One of the best experts on this subject based on the ideXlab platform.
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evolution of phage type rna polymerases in higher plants characterization of the single phage type rna polymerase gene from Selaginella moellendorffii
Journal of Molecular Evolution, 2009Co-Authors: Chang Yin, Uwe Richter, Thomas Borner, Andreas WeiheAbstract:Selaginella moellendorfii (spikemoss) sequence trace data encoding a polypeptide highly similar to angiosperm and moss phage-type organelle RNA polymerases (RpoTs) were used to isolate a BAC clone containing the full-length gene SmRpoT as well as the corresponding cDNA. The SmRpoT mRNA comprises 3452 nt with an open reading frame of 3006 nt, encoding a putative protein of 1002 amino acids with a molecular mass of 113 kDa. The SmRpoT gene comprises 19 exons and 18 introns, conserved in their position with those of the angiosperm and Physcomitrella RpoT genes. In phylogenetic analyses, the Selaginella RpoT polymerase is in a sister position to all other phage-type polymerases of angiosperms. However, according to its conserved exon–intron structure, the Selaginella RpoT gene is representative of the molecular evolutionary lineage giving rise to the RpoT gene family of flowering plants. The N-terminal transit peptide of SmRpoT is shown to confer targeting of green fluorescent protein exclusively to mitochondria after transient expression in Arabidopsis and Selaginella protoplasts. Angiosperms and the moss P. patens possess small gene families encoding RpoTs, which include mitochondrial- and chloroplast-targeted RNA polymerases. In striking contrast, the Selaginella RpoT gene is shown to be single-copy, although Selaginella, as a lycophyte, has a phylogenetic position between Physcomitrella and angiosperms. Thus, there is no evidence that Selaginella may contain a nuclear-encoded phage-type chloroplast RNA polymerase.
Yao Xin-sheng - One of the best experts on this subject based on the ideXlab platform.
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Isolation and identification of phenolic constituents from Selaginella uncinata(Desv.) Spring
Chinese Journal of Medicinal Chemistry, 2007Co-Authors: Yao Xin-shengAbstract:Aim To study chemical constituents of Selaginella uncinata(Desv.)Spring.Methods The compounds were isolated by means of chromatography on silica gel,Sephadex LH-20 column,ODS column,and reversed-phase Pre-HPLC.Their structures were elucidated by spectroscopic analysis and chemical methods.Results and conclusion Seven phenolic compounds were obtained from the 60% ethanol extract of Selaginella uncinata(Desv.)Spring and their structures were identified as 16-feruloyloxypalmitic acid(1),p-hydroxycinnamic acid(2),1-(4-hydroxyphenyl)-ethanone(3),vanillin(4),vanillic acid(5),syringic acid(6),p-hydroxybenzoic acid(7).Compounds 1-3 were isolated from Selaginella genus for the first time,while compounds 1-7 were firstly obtained from Selaginella uncinata(Desv.)Spring.
Wolf B. Frommer - One of the best experts on this subject based on the ideXlab platform.
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Amino Acid transporter inventory of the Selaginella genome.
Frontiers in plant science, 2012Co-Authors: Daniel Wipf, Dominique Loqué, Sylvie Lalonde, Wolf B. FrommerAbstract:Amino acids play fundamental roles in a multitude of functions including protein synthesis, hormone metabolism, nerve transmission, cell growth, production of metabolic energy, nucleobase synthesis, nitrogen metabolism and urea biosynthesis. Selaginella as a member of the lycophytes is part of an ancient lineage of vascular plants that had arisen ~400 million years ago. In angiosperms, which have attracted most of the attention for nutrient distribution so far, we have been able to identify many of the key transporters for nitrogen. Their role is not always fully clear, thus an analysis of Selaginella as a representative of an ancient vascular plant may help shedding light on the evolution and function of these diverse transporters. Here we analyze the genes for transporters involved in cellular uptake of amino acids present in the Selaginella genome.
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Amino acid transporter inventory of the Selaginella genome
Frontiers in Plant Science, 2012Co-Authors: Daniel Wipf, Dominique Loqué, Sylvie Lalonde, Wolf B. FrommerAbstract:Amino acids play fundamental roles in a multitude of functions including protein synthesis, hormone metabolism, nerve transmission, cell growth, production of metabolic energy, nucleobase synthesis, nitrogen metabolism, and urea biosynthesis. Selaginella as a member of the lycophytes is part of an ancient lineage of vascular plants that had arisen 400 million years ago. In angiosperms, which have attracted most of the attention for nutrient transport so far, we have been able to identify many of the key transporters for nitrogen. Their role is not always fully clear, thus an analysis of Selaginella as a representative of an ancient vascular plant may help shed light on the evolution and function of these diverse transporters. Here we annotated and analyzed the genes encoding putative transporters involved in cellular uptake of amino acids present in the Selaginella genome
Jo Ann Banks - One of the best experts on this subject based on the ideXlab platform.
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The Selaginella Genome Identifies Genetic Changes Associated with the Evolution of Vascular Plants
Science, 2011Co-Authors: Jo Ann Banks, Tomoaki Nishiyama, Mitsuyasu Hasebe, John Bowman, Michael Gribskov, Claude De Pamphilis, Victor Albert, Naoki Aono, Tsuyoshi Aoyama, Barbara AmbroseAbstract:Vascular plants appeared ~410 million years ago, then diverged into several lineages of which only two survive: the euphyllophytes (ferns and seed plants) and the lycophytes. We report here the genome sequence of the lycophyte Selaginella moellendorffii (Selaginella), the first nonseed vascular plant genome reported. By comparing gene content in evolutionarily diverse taxa, we found that the transition from a gametophyte- to a sporophyte-dominated life cycle required far fewer new genes than the transition from a nonseed vascular to a flowering plant, whereas secondary metabolic genes expanded extensively and in parallel in the lycophyte and angiosperm lineages. Selaginella differs in posttranscriptional gene regulation, including small RNA regulation of repetitive elements, an absence of the trans-acting small interfering RNA pathway, and extensive RNA editing of organellar genes.
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Selaginella and 400 Million Years of Separation
Annual Review of Plant Biology, 2009Co-Authors: Jo Ann BanksAbstract:Selaginella (spikemoss) is an enigma in the plant kingdom. Although a fascination to botanists at the turn of the twentieth century, members of this genus are unremarkable in appearance, never flower, and are of no agronomic value. However, members of this genus are relicts from ancient times, and one has to marvel at how this genus has survived virtually unchanged in appearance for hundreds of millions of years. In light of the recent completion of the Selaginella moellendorffii genome sequence, this review is intended to survey what is known about Selaginella, with a special emphasis on recent inquiries into its unique biology and importance in understanding the early evolution of vascular plants.
Chang Yin - One of the best experts on this subject based on the ideXlab platform.
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evolution of phage type rna polymerases in higher plants characterization of the single phage type rna polymerase gene from Selaginella moellendorffii
Journal of Molecular Evolution, 2009Co-Authors: Chang Yin, Uwe Richter, Thomas Borner, Andreas WeiheAbstract:Selaginella moellendorfii (spikemoss) sequence trace data encoding a polypeptide highly similar to angiosperm and moss phage-type organelle RNA polymerases (RpoTs) were used to isolate a BAC clone containing the full-length gene SmRpoT as well as the corresponding cDNA. The SmRpoT mRNA comprises 3452 nt with an open reading frame of 3006 nt, encoding a putative protein of 1002 amino acids with a molecular mass of 113 kDa. The SmRpoT gene comprises 19 exons and 18 introns, conserved in their position with those of the angiosperm and Physcomitrella RpoT genes. In phylogenetic analyses, the Selaginella RpoT polymerase is in a sister position to all other phage-type polymerases of angiosperms. However, according to its conserved exon–intron structure, the Selaginella RpoT gene is representative of the molecular evolutionary lineage giving rise to the RpoT gene family of flowering plants. The N-terminal transit peptide of SmRpoT is shown to confer targeting of green fluorescent protein exclusively to mitochondria after transient expression in Arabidopsis and Selaginella protoplasts. Angiosperms and the moss P. patens possess small gene families encoding RpoTs, which include mitochondrial- and chloroplast-targeted RNA polymerases. In striking contrast, the Selaginella RpoT gene is shown to be single-copy, although Selaginella, as a lycophyte, has a phylogenetic position between Physcomitrella and angiosperms. Thus, there is no evidence that Selaginella may contain a nuclear-encoded phage-type chloroplast RNA polymerase.
