The Experts below are selected from a list of 117 Experts worldwide ranked by ideXlab platform
Zhizeng Wang - One of the best experts on this subject based on the ideXlab platform.
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a point of care selenium nanoparticle based test for the combined detection of anti sars cov 2 igm and igg in human serum and blood
Lab on a Chip, 2020Co-Authors: Zhizeng Wang, Zhi Zheng, Qianwen Zhou, Wei Liu, Zhigang Liu, Yaohui WangAbstract:COVID-19 is a widespread and highly contagious disease in the human population. COVID-19 is caused by SARS-CoV-2 infection. There is still a great demand for point-of-care tests for detection, epidemic prevention and epidemiological investigation, both now and after the epidemic. We present a lateral flow immunoassay kit based on a selenium nanoparticle-modified SARS-CoV-2 nucleoprotein, which detects anti-SARS-CoV-2 IgM and anti-SARS-CoV-2 IgG in human serum, and the results can be read by the naked eye in 10 minutes. We expressed and purified the SARS-CoV-2 nucleoprotein in HEK293 cells, with a purity of 98.14% and a concentration of 5 mg mL-1. Selenium nanoparticles were synthesized by l-ascorbic Acid reduction of Seleninic Acid at room temperature. After conjugation with the nucleoprotein, a lateral flow kit was successfully prepared. The IgM and IgG detection limits of the lateral flow kit reached 20 ng mL-1 and 5 ng mL-1, respectively, in human serum. A clinical study sample comprising 90 COVID-19-diagnosed patients and 263 non-infected controls was used to demonstrate a sensitivity and specificity of 93.33% and 97.34%, respectively, based on RT-PCR and clinical results. No cross-reactions with rheumatoid factor and positive serum for anti-nuclear antibodies, influenza A, and influenza B were observed. Moreover, the lateral flow kit remained stable after storage for 30 days at 37 °C. Our results demonstrate that the selenium nanoparticle lateral flow kit can conveniently, rapidly, and sensitively detect anti-SARS-CoV-2 IgM and IgG in human serum and blood; it can also be suitable for the epidemiological investigation of COVID-19.
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lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk milk powder and animal feed
International Journal of Nanomedicine, 2014Co-Authors: Zhizeng Wang, Dejuan Zhi, Hailong Zhang, Yang Lu-zhao, Xin WangAbstract:Although high melamine (MEL) intake has been proven to cause serious health problems, MEL is sometimes illegally added to milk products and animal feed, arousing serious food safety concerns. A satisfactory method of detecting MEL in onsite or in-home testing is in urgent need of development. This work aimed to explore a rapid, convenient, and cost-effective method of identifying MEL in milk products or other food by colloidal selenium-based lateral flow immunoassay. Colloidal selenium was synthesized by L-ascorbic Acid to reduce Seleninic Acid at room temperature. After conjugation with a monoclonal antibody anti-MEL, a test strip was successfully prepared. The detection limit of the test strip reached 150 μg/kg, 1,000 μg/kg, and 800 μg/kg in liquid milk, milk powder, and animal feed, respectively. No cross-reactions with homologues cyanuric Acid, cyanurodiamide, or ammelide were found. Moreover, the MEL test strip can remain stable after storage for 1 year at room temperature. Our results demonstrate that the colloidal selenium MEL test strip can detect MEL in adulterated milk products or animal feed conveniently, rapidly, and sensitively. In contrast with a colloidal gold MEL test strip, the colloidal selenium MEL test strip was easy to prepare and more cost-efficient.
Xin Wang - One of the best experts on this subject based on the ideXlab platform.
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lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk milk powder and animal feed
International Journal of Nanomedicine, 2014Co-Authors: Zhizeng Wang, Dejuan Zhi, Hailong Zhang, Yang Lu-zhao, Xin WangAbstract:Although high melamine (MEL) intake has been proven to cause serious health problems, MEL is sometimes illegally added to milk products and animal feed, arousing serious food safety concerns. A satisfactory method of detecting MEL in onsite or in-home testing is in urgent need of development. This work aimed to explore a rapid, convenient, and cost-effective method of identifying MEL in milk products or other food by colloidal selenium-based lateral flow immunoassay. Colloidal selenium was synthesized by L-ascorbic Acid to reduce Seleninic Acid at room temperature. After conjugation with a monoclonal antibody anti-MEL, a test strip was successfully prepared. The detection limit of the test strip reached 150 μg/kg, 1,000 μg/kg, and 800 μg/kg in liquid milk, milk powder, and animal feed, respectively. No cross-reactions with homologues cyanuric Acid, cyanurodiamide, or ammelide were found. Moreover, the MEL test strip can remain stable after storage for 1 year at room temperature. Our results demonstrate that the colloidal selenium MEL test strip can detect MEL in adulterated milk products or animal feed conveniently, rapidly, and sensitively. In contrast with a colloidal gold MEL test strip, the colloidal selenium MEL test strip was easy to prepare and more cost-efficient.
Isabel W C E Arends - One of the best experts on this subject based on the ideXlab platform.
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epoxidation of cyclooctene and cyclohexene with hydrogen peroxide catalyzed by bis 3 5 bis trifluoromethyl diphenyl diselenide recyclable catalyst containing phases through the use of glycerol derived solvents
Journal of Molecular Catalysis A-chemical, 2011Co-Authors: Hector Garciamarin, John C Van Der Toorn, Jose A Mayoral, Jose I Garcia, Isabel W C E ArendsAbstract:A green strategy has been explored for olefin epoxidation which combines renewable solvents (derived from glycerol), aqueous hydrogen peroxide, and catalyst recycling (a Seleninic Acid derivative). The use of fluorinated glycerol derivatives allows good catalytic activity in the epoxidation of cyclooctene and cyclohexene with aqueous hydrogen peroxide, preventing epoxide hydrolysis to a great extent, which is particularly remarkable in the case of cyclohexene. Furthermore, recycling of the catalytically active phase is possible through distillation of the cyclohexene oxide from the reaction mixture, which can be subsequently recharged with fresh oxidant and substrate.
Hector Garciamarin - One of the best experts on this subject based on the ideXlab platform.
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epoxidation of cyclooctene and cyclohexene with hydrogen peroxide catalyzed by bis 3 5 bis trifluoromethyl diphenyl diselenide recyclable catalyst containing phases through the use of glycerol derived solvents
Journal of Molecular Catalysis A-chemical, 2011Co-Authors: Hector Garciamarin, John C Van Der Toorn, Jose A Mayoral, Jose I Garcia, Isabel W C E ArendsAbstract:A green strategy has been explored for olefin epoxidation which combines renewable solvents (derived from glycerol), aqueous hydrogen peroxide, and catalyst recycling (a Seleninic Acid derivative). The use of fluorinated glycerol derivatives allows good catalytic activity in the epoxidation of cyclooctene and cyclohexene with aqueous hydrogen peroxide, preventing epoxide hydrolysis to a great extent, which is particularly remarkable in the case of cyclohexene. Furthermore, recycling of the catalytically active phase is possible through distillation of the cyclohexene oxide from the reaction mixture, which can be subsequently recharged with fresh oxidant and substrate.
Yang Lu-zhao - One of the best experts on this subject based on the ideXlab platform.
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lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk milk powder and animal feed
International Journal of Nanomedicine, 2014Co-Authors: Zhizeng Wang, Dejuan Zhi, Hailong Zhang, Yang Lu-zhao, Xin WangAbstract:Although high melamine (MEL) intake has been proven to cause serious health problems, MEL is sometimes illegally added to milk products and animal feed, arousing serious food safety concerns. A satisfactory method of detecting MEL in onsite or in-home testing is in urgent need of development. This work aimed to explore a rapid, convenient, and cost-effective method of identifying MEL in milk products or other food by colloidal selenium-based lateral flow immunoassay. Colloidal selenium was synthesized by L-ascorbic Acid to reduce Seleninic Acid at room temperature. After conjugation with a monoclonal antibody anti-MEL, a test strip was successfully prepared. The detection limit of the test strip reached 150 μg/kg, 1,000 μg/kg, and 800 μg/kg in liquid milk, milk powder, and animal feed, respectively. No cross-reactions with homologues cyanuric Acid, cyanurodiamide, or ammelide were found. Moreover, the MEL test strip can remain stable after storage for 1 year at room temperature. Our results demonstrate that the colloidal selenium MEL test strip can detect MEL in adulterated milk products or animal feed conveniently, rapidly, and sensitively. In contrast with a colloidal gold MEL test strip, the colloidal selenium MEL test strip was easy to prepare and more cost-efficient.