The Experts below are selected from a list of 207 Experts worldwide ranked by ideXlab platform
Javier Castillo - One of the best experts on this subject based on the ideXlab platform.
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Comparison of several Real-Time PCR Kits versus a Culture-dependent Algorithm to Identify Enteropathogens in Stool Samples
Scientific Reports, 2020Co-Authors: Silvia Valledor, Inés Valledor, María Concepción Gil-rodríguez, Cristina Seral, Javier CastilloAbstract:This study aims to validate the current diagnostic method for the clinical detection of gastroenteritis. We analyzed 400 stool samples to detect three of the most common enteropathogens: Salmonella spp., Campylobacter spp., and Yersinia enterocolitica . All specimens were tested with a routine clinical diagnosis algorithm and with five real-time PCR assays. A total of 98 specimens (24.5%) were positive for enteropathogens. We found 24 samples positive for Salmonella enterica , 71 positive for Campylobacter spp., and 4 positive for Yersinia enterocolitica . All evaluated methods exhibited a good performance in identifying Salmonella and Yersinia enterocolitica , being the highest positive percent agreement (PPA) value of 95.8% and 100%, respectively. The clinical algorithm showed the highest PPA value identifying Salmonella , due to the enrichment in Selenite Broth. However, the evaluated methods showed notable differences in the identification of Campylobacter species, obtaining a wide range of PPA values: 59.2%–100%. The clinical algorithm showed the lowest PPA value since it was only able to detect Campylobacter jejuni and Campylobacter coli species. This study revealed the importance of implementing the real-time PCR technique in a clinical algorithm: it improved the accuracy of the diagnosis and provided results in a shorter time compared to routine clinical methods.
L. Martinez Campos - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of Methods for Isolation of Salmonella Species using Modified Semisolid Rappaport-Vassiliadis Medium and Salmonella-Shigella Agar
European Journal of Clinical Microbiology and Infectious Diseases, 1998Co-Authors: J. Ruiz Gomez, I. Lorente Salinas, J. Perez Salmerón, E. Simarro Cordoba, L. Martinez CamposAbstract:A total of 197 Salmonella strains were isolated from 1717 stool samples on salmonella-shigella agar and modified semisolid Rappaport-Vassiliadis medium before and after enrichment in Selenite Broth. Better sensitivity was obtained with salmonella-shigella agar than in direct plating (89.2% vs. 64.4%, P
F K Gould - One of the best experts on this subject based on the ideXlab platform.
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abc medium a new chromogenic agar for selective isolation of salmonella spp
Journal of Clinical Microbiology, 1999Co-Authors: John D Perry, Michael Ford, Jeffrey Taylor, Amanda Jones, Roger Freeman, F K GouldAbstract:We describe a new chromogenic agar medium, ABC medium (αβ-chromogenic medium), which includes two substrates, 3,4-cyclohexenoesculetin-β-d-galactoside and 5-bromo-4-chloro-3-indolyl-α-d-galactopyranoside, to facilitate the selective isolation of Salmonella spp. This medium exploits the fact that Salmonella spp. may be distinguished from other members of the family Enterobacteriaceae by the presence of α-galactosidase activity in the absence of β-galactosidase activity. A total of 1,022 strains of Salmonella spp. and 300 other gram-negative strains were inoculated onto this medium. Of these, 1,019 (99.7%) strains of Salmonella spp. produced a characteristic green colony, whereas only 1 strain (0.33%) of non-Salmonella produced a green colony. A total of 283 stool samples were cultured onto desoxycholate citrate (DC) agar and ABC medium by direct inoculation and after selective enrichment in Selenite Broth. Overall, the sensitivity and specificity were superior for ABC medium (100 and 90.5%, respectively) than for DC agar (88 and 26.9%, respectively). We conclude that ABC medium offers a high degree of specificity for the detection of Salmonella spp. in stool samples.
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ABC Medium, a New Chromogenic Agar for Selective Isolation of Salmonella spp.
Journal of clinical microbiology, 1999Co-Authors: John D Perry, Michael Ford, Jeffrey Taylor, Amanda Jones, Roger Freeman, F K GouldAbstract:We describe a new chromogenic agar medium, ABC medium (alphabeta-chromogenic medium), which includes two substrates, 3, 4-cyclohexenoesculetin-beta-D-galactoside and 5-bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside, to facilitate the selective isolation of Salmonella spp. This medium exploits the fact that Salmonella spp. may be distinguished from other members of the family Enterobacteriaceae by the presence of alpha-galactosidase activity in the absence of beta-galactosidase activity. A total of 1, 022 strains of Salmonella spp. and 300 other gram-negative strains were inoculated onto this medium. Of these, 1,019 (99.7%) strains of Salmonella spp. produced a characteristic green colony, whereas only 1 strain (0.33%) of non-Salmonella produced a green colony. A total of 283 stool samples were cultured onto desoxycholate citrate (DC) agar and ABC medium by direct inoculation and after selective enrichment in Selenite Broth. Overall, the sensitivity and specificity were superior for ABC medium (100 and 90.5%, respectively) than for DC agar (88 and 26.9%, respectively). We conclude that ABC medium offers a high degree of specificity for the detection of Salmonella spp. in stool samples.
Silvia Valledor - One of the best experts on this subject based on the ideXlab platform.
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Comparison of several Real-Time PCR Kits versus a Culture-dependent Algorithm to Identify Enteropathogens in Stool Samples
Scientific Reports, 2020Co-Authors: Silvia Valledor, Inés Valledor, María Concepción Gil-rodríguez, Cristina Seral, Javier CastilloAbstract:This study aims to validate the current diagnostic method for the clinical detection of gastroenteritis. We analyzed 400 stool samples to detect three of the most common enteropathogens: Salmonella spp., Campylobacter spp., and Yersinia enterocolitica . All specimens were tested with a routine clinical diagnosis algorithm and with five real-time PCR assays. A total of 98 specimens (24.5%) were positive for enteropathogens. We found 24 samples positive for Salmonella enterica , 71 positive for Campylobacter spp., and 4 positive for Yersinia enterocolitica . All evaluated methods exhibited a good performance in identifying Salmonella and Yersinia enterocolitica , being the highest positive percent agreement (PPA) value of 95.8% and 100%, respectively. The clinical algorithm showed the highest PPA value identifying Salmonella , due to the enrichment in Selenite Broth. However, the evaluated methods showed notable differences in the identification of Campylobacter species, obtaining a wide range of PPA values: 59.2%–100%. The clinical algorithm showed the lowest PPA value since it was only able to detect Campylobacter jejuni and Campylobacter coli species. This study revealed the importance of implementing the real-time PCR technique in a clinical algorithm: it improved the accuracy of the diagnosis and provided results in a shorter time compared to routine clinical methods.
Carl J Mason - One of the best experts on this subject based on the ideXlab platform.
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efficiency of plating media and enrichment Broths for isolating salmonella species from human stool samples a comparison study
Open Journal of Medical Microbiology, 2015Co-Authors: Apichai Srijan, Boonchai Wongstitwilairoong, Ladaporn Bodhidatta, Carl J MasonAbstract:A comparative study was performed to evaluate best practice culture media and enrichment Broths for recovering Salmonella species from human stool samples. A total of 1297 human stools were collected and processed in this study. Evaluation of agar media was carried out by direct plating (DP), 1096 stool samples were inoculated on Modified Semisolid Rappaport-Vassiliadis (MSRV), Xylose-Lysine-Deoxycolate (XLD), MacConkey (MAC), and Hektoen Enteric (HE) agars. Evaluation of enrichment Broths were carried out by enrichment all 1297 stool samples in Selenite Broth (SB), Rappaport-Vassiliadis (RV) Broth, and Buffered Peptone Water (BPW), followed by plating on MSRV, MAC, and HE agars. A total of 102 Salmonella-positive stools by DP, 85.3% (87/102) were recovered utilizing MSRV while recovery from XLD, MAC, and HE agars were 34.3% (35/102), 34.3% (35/102), and 29.4% (30/102) respectively. A total 299/1297 stools samples were Salmonella-positive on at least one plating medium after enrichment procedure were 77.3% (177/299) for SB, 86.0% (197/299) and 78.6% (180/299) for RV and BPW respectively. All Salmonella isolated in this study was nontyphi Salmonella. Presently, the data suggest that the use of MSRV over MAC, HE, and XLD agars for isolation nontyphi Salmonella species from human stools is more efficacious. Additionally, use of MSRV in combination with MAC and HE agars following enrichment in RV Broth enhances recovery of nontyphi Salmonella species. However, RV Broth is inhibitory to typhi Salmonella, thus use of MSRV medium in combination with MAC, HE or XLD agars in direct plating following enrichment in non-selective BPW is an alternate method for recovery of both typhi and nontyphi Salmonella species contaminated in human stool samples.
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Efficiency of Plating Media and Enrichment Broths for Isolating Salmonella Species from Human Stool Samples: A Comparison Study
Open Journal of Medical Microbiology, 2015Co-Authors: Apichai Srijan, Boonchai Wongstitwilairoong, Ladaporn Bodhidatta, Carl J MasonAbstract:A comparative study was performed to evaluate best practice culture media and enrichment Broths for recovering Salmonella species from human stool samples. A total of 1297 human stools were collected and processed in this study. Evaluation of agar media was carried out by direct plating (DP), 1096 stool samples were inoculated on Modified Semisolid Rappaport-Vassiliadis (MSRV), Xylose-Lysine-Deoxycolate (XLD), MacConkey (MAC), and Hektoen Enteric (HE) agars. Evaluation of enrichment Broths were carried out by enrichment all 1297 stool samples in Selenite Broth (SB), Rappaport-Vassiliadis (RV) Broth, and Buffered Peptone Water (BPW), followed by plating on MSRV, MAC, and HE agars. A total of 102 Salmonella-positive stools by DP, 85.3% (87/102) were recovered utilizing MSRV while recovery from XLD, MAC, and HE agars were 34.3% (35/102), 34.3% (35/102), and 29.4% (30/102) respectively. A total 299/1297 stools samples were Salmonella-positive on at least one plating medium after enrichment procedure were 77.3% (177/299) for SB, 86.0% (197/299) and 78.6% (180/299) for RV and BPW respectively. All Salmonella isolated in this study was nontyphi Salmonella. Presently, the data suggest that the use of MSRV over MAC, HE, and XLD agars for isolation nontyphi Salmonella species from human stools is more efficacious. Additionally, use of MSRV in combination with MAC and HE agars following enrichment in RV Broth enhances recovery of nontyphi Salmonella species. However, RV Broth is inhibitory to typhi Salmonella, thus use of MSRV medium in combination with MAC, HE or XLD agars in direct plating following enrichment in non-selective BPW is an alternate method for recovery of both typhi and nontyphi Salmonella species contaminated in human stool samples.