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Joon-seok Chae - One of the best experts on this subject based on the ideXlab platform.
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Identification of trematode cercariae carrying Neorickettsia risticii in freshwater stream snails.
Annals of the New York Academy of Sciences, 2003Co-Authors: Bae Keun Park, Meen‐ju Kim, Eun-ha Kim, Min-seok Kim, Joon-seok ChaeAbstract:: We provide evidence of Neorickettsia (Ehrlichia) risticii Holland, the agent of Potomac horse fever, in trematode larval stages found in aquatic snails and insects collected from a stream in Korea, using nested polymerase chain reaction (PCR) and sequence analysis of the 16S rRNA gene fragment amplified from several cercaria species. It was observed that of 423 (13.1%) of 3,219 snails infected with cercariae, 77 (20.8% of the 371) were infected with N. risticii. Five families of trematode cercariae, Schistosomatidae, Echimostomatidae, Heterophyidae, Microphallidae, and Acanthocopidae were identified morphologically within Semisulcospira libertina, Radix auricularia coreana, and S. gottschei snails. Echinostoma cinetorchis, E. hortense, and Metagonimus sp. were identified based on both the cercarial morphology as well as by phylogenetic analysis of the amplified 18S rRNA gene sequences. Adult aquatic insects were also collected from the same sites and were sorted into five species, Ischnura asiatica in Coenagrionidae and Calopteryx japonica, Sympetrum darwinianum, Symptrum eroticum, and Symptrum parvulum in Calopterygoidae. One thousand and two hundred eighty five metacercariae (classified into groups A through F) were isolated from 310 adult aquatic insects, and the average number of metacercariae per aquatic insect was 4.1. However, there was no amplification of N. risticii from these metacercariae.
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Prevalence and sequence analyses of Neorickettsia risticii.
Annals of the New York Academy of Sciences, 2003Co-Authors: Joon-seok Chae, Eun-ha Kim, Min-seok Kim, Minju Kim, Yoon-hee Cho, Bae-kuen ParkAbstract:: The presence of Neorickettsia (Ehrlichia) risticii DNA was confirmed by PCR amplification and sequence analysis from cercaria in snails collected from stream water in Chungcheong and Jeonra provinces. A total of 3,219 snails were tested for trematode cercariae and N. risticii infection. N. risticii 16S rRNA gene fragment was amplified in cercariae from Semisulcospira libertina and Radix auricularia coreana snails by nested PCR. More than four genus cercariae (Schistosomatidae, Microphallidae, Furcocercus, and Xiphidiocercaria) as well as unidentified cercariae were found from Semisulcospira libertina snails. Three species of cercariae (E. cinetorichis, E. hortense, and Fasciola sp.) were found from Radix auricularia coreana snails. The cercariae were present in 429 (13.3%) snails of 3,216 collected at the Chungcheong and Jeonra provinces. The prevalence of N. risticii in these 429 cercariae was 17.9% (77 of 429 cercariae-infected snails). The amplicons of N. risticii 16S rRNA gene fragment (527 bp) from cercariae DNA had two genotypes (NR-JA1 and NR-JA2) with an identity of 96.4% between their nucleotide sequences. However, when compared to the sequence of N. risticii Shasta strain these sequences showed a 94.3% and 96.4% homology, respectively. The comparison of N. risticii 51 kDa major antigen gene sequences (572 bp) from NR-JA1 and NR-JA2 were 100% identical to the sequence of the isolates from Juga sp., Caddisfly larvae, Shasta, Juga yrekaensis, and trematode of California. This study reports for the first time the detection of N. risticii from cercariae found in Radix auricularia coreana snail. These data also indicate that N. risticii could be widespread in Korea.
Min-seok Kim - One of the best experts on this subject based on the ideXlab platform.
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Identification of trematode cercariae carrying Neorickettsia risticii in freshwater stream snails.
Annals of the New York Academy of Sciences, 2003Co-Authors: Bae Keun Park, Meen‐ju Kim, Eun-ha Kim, Min-seok Kim, Joon-seok ChaeAbstract:: We provide evidence of Neorickettsia (Ehrlichia) risticii Holland, the agent of Potomac horse fever, in trematode larval stages found in aquatic snails and insects collected from a stream in Korea, using nested polymerase chain reaction (PCR) and sequence analysis of the 16S rRNA gene fragment amplified from several cercaria species. It was observed that of 423 (13.1%) of 3,219 snails infected with cercariae, 77 (20.8% of the 371) were infected with N. risticii. Five families of trematode cercariae, Schistosomatidae, Echimostomatidae, Heterophyidae, Microphallidae, and Acanthocopidae were identified morphologically within Semisulcospira libertina, Radix auricularia coreana, and S. gottschei snails. Echinostoma cinetorchis, E. hortense, and Metagonimus sp. were identified based on both the cercarial morphology as well as by phylogenetic analysis of the amplified 18S rRNA gene sequences. Adult aquatic insects were also collected from the same sites and were sorted into five species, Ischnura asiatica in Coenagrionidae and Calopteryx japonica, Sympetrum darwinianum, Symptrum eroticum, and Symptrum parvulum in Calopterygoidae. One thousand and two hundred eighty five metacercariae (classified into groups A through F) were isolated from 310 adult aquatic insects, and the average number of metacercariae per aquatic insect was 4.1. However, there was no amplification of N. risticii from these metacercariae.
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Prevalence and sequence analyses of Neorickettsia risticii.
Annals of the New York Academy of Sciences, 2003Co-Authors: Joon-seok Chae, Eun-ha Kim, Min-seok Kim, Minju Kim, Yoon-hee Cho, Bae-kuen ParkAbstract:: The presence of Neorickettsia (Ehrlichia) risticii DNA was confirmed by PCR amplification and sequence analysis from cercaria in snails collected from stream water in Chungcheong and Jeonra provinces. A total of 3,219 snails were tested for trematode cercariae and N. risticii infection. N. risticii 16S rRNA gene fragment was amplified in cercariae from Semisulcospira libertina and Radix auricularia coreana snails by nested PCR. More than four genus cercariae (Schistosomatidae, Microphallidae, Furcocercus, and Xiphidiocercaria) as well as unidentified cercariae were found from Semisulcospira libertina snails. Three species of cercariae (E. cinetorichis, E. hortense, and Fasciola sp.) were found from Radix auricularia coreana snails. The cercariae were present in 429 (13.3%) snails of 3,216 collected at the Chungcheong and Jeonra provinces. The prevalence of N. risticii in these 429 cercariae was 17.9% (77 of 429 cercariae-infected snails). The amplicons of N. risticii 16S rRNA gene fragment (527 bp) from cercariae DNA had two genotypes (NR-JA1 and NR-JA2) with an identity of 96.4% between their nucleotide sequences. However, when compared to the sequence of N. risticii Shasta strain these sequences showed a 94.3% and 96.4% homology, respectively. The comparison of N. risticii 51 kDa major antigen gene sequences (572 bp) from NR-JA1 and NR-JA2 were 100% identical to the sequence of the isolates from Juga sp., Caddisfly larvae, Shasta, Juga yrekaensis, and trematode of California. This study reports for the first time the detection of N. risticii from cercariae found in Radix auricularia coreana snail. These data also indicate that N. risticii could be widespread in Korea.
Eun-ha Kim - One of the best experts on this subject based on the ideXlab platform.
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Identification of trematode cercariae carrying Neorickettsia risticii in freshwater stream snails.
Annals of the New York Academy of Sciences, 2003Co-Authors: Bae Keun Park, Meen‐ju Kim, Eun-ha Kim, Min-seok Kim, Joon-seok ChaeAbstract:: We provide evidence of Neorickettsia (Ehrlichia) risticii Holland, the agent of Potomac horse fever, in trematode larval stages found in aquatic snails and insects collected from a stream in Korea, using nested polymerase chain reaction (PCR) and sequence analysis of the 16S rRNA gene fragment amplified from several cercaria species. It was observed that of 423 (13.1%) of 3,219 snails infected with cercariae, 77 (20.8% of the 371) were infected with N. risticii. Five families of trematode cercariae, Schistosomatidae, Echimostomatidae, Heterophyidae, Microphallidae, and Acanthocopidae were identified morphologically within Semisulcospira libertina, Radix auricularia coreana, and S. gottschei snails. Echinostoma cinetorchis, E. hortense, and Metagonimus sp. were identified based on both the cercarial morphology as well as by phylogenetic analysis of the amplified 18S rRNA gene sequences. Adult aquatic insects were also collected from the same sites and were sorted into five species, Ischnura asiatica in Coenagrionidae and Calopteryx japonica, Sympetrum darwinianum, Symptrum eroticum, and Symptrum parvulum in Calopterygoidae. One thousand and two hundred eighty five metacercariae (classified into groups A through F) were isolated from 310 adult aquatic insects, and the average number of metacercariae per aquatic insect was 4.1. However, there was no amplification of N. risticii from these metacercariae.
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Prevalence and sequence analyses of Neorickettsia risticii.
Annals of the New York Academy of Sciences, 2003Co-Authors: Joon-seok Chae, Eun-ha Kim, Min-seok Kim, Minju Kim, Yoon-hee Cho, Bae-kuen ParkAbstract:: The presence of Neorickettsia (Ehrlichia) risticii DNA was confirmed by PCR amplification and sequence analysis from cercaria in snails collected from stream water in Chungcheong and Jeonra provinces. A total of 3,219 snails were tested for trematode cercariae and N. risticii infection. N. risticii 16S rRNA gene fragment was amplified in cercariae from Semisulcospira libertina and Radix auricularia coreana snails by nested PCR. More than four genus cercariae (Schistosomatidae, Microphallidae, Furcocercus, and Xiphidiocercaria) as well as unidentified cercariae were found from Semisulcospira libertina snails. Three species of cercariae (E. cinetorichis, E. hortense, and Fasciola sp.) were found from Radix auricularia coreana snails. The cercariae were present in 429 (13.3%) snails of 3,216 collected at the Chungcheong and Jeonra provinces. The prevalence of N. risticii in these 429 cercariae was 17.9% (77 of 429 cercariae-infected snails). The amplicons of N. risticii 16S rRNA gene fragment (527 bp) from cercariae DNA had two genotypes (NR-JA1 and NR-JA2) with an identity of 96.4% between their nucleotide sequences. However, when compared to the sequence of N. risticii Shasta strain these sequences showed a 94.3% and 96.4% homology, respectively. The comparison of N. risticii 51 kDa major antigen gene sequences (572 bp) from NR-JA1 and NR-JA2 were 100% identical to the sequence of the isolates from Juga sp., Caddisfly larvae, Shasta, Juga yrekaensis, and trematode of California. This study reports for the first time the detection of N. risticii from cercariae found in Radix auricularia coreana snail. These data also indicate that N. risticii could be widespread in Korea.
Bae-kuen Park - One of the best experts on this subject based on the ideXlab platform.
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Prevalence and sequence analyses of Neorickettsia risticii.
Annals of the New York Academy of Sciences, 2003Co-Authors: Joon-seok Chae, Eun-ha Kim, Min-seok Kim, Minju Kim, Yoon-hee Cho, Bae-kuen ParkAbstract:: The presence of Neorickettsia (Ehrlichia) risticii DNA was confirmed by PCR amplification and sequence analysis from cercaria in snails collected from stream water in Chungcheong and Jeonra provinces. A total of 3,219 snails were tested for trematode cercariae and N. risticii infection. N. risticii 16S rRNA gene fragment was amplified in cercariae from Semisulcospira libertina and Radix auricularia coreana snails by nested PCR. More than four genus cercariae (Schistosomatidae, Microphallidae, Furcocercus, and Xiphidiocercaria) as well as unidentified cercariae were found from Semisulcospira libertina snails. Three species of cercariae (E. cinetorichis, E. hortense, and Fasciola sp.) were found from Radix auricularia coreana snails. The cercariae were present in 429 (13.3%) snails of 3,216 collected at the Chungcheong and Jeonra provinces. The prevalence of N. risticii in these 429 cercariae was 17.9% (77 of 429 cercariae-infected snails). The amplicons of N. risticii 16S rRNA gene fragment (527 bp) from cercariae DNA had two genotypes (NR-JA1 and NR-JA2) with an identity of 96.4% between their nucleotide sequences. However, when compared to the sequence of N. risticii Shasta strain these sequences showed a 94.3% and 96.4% homology, respectively. The comparison of N. risticii 51 kDa major antigen gene sequences (572 bp) from NR-JA1 and NR-JA2 were 100% identical to the sequence of the isolates from Juga sp., Caddisfly larvae, Shasta, Juga yrekaensis, and trematode of California. This study reports for the first time the detection of N. risticii from cercariae found in Radix auricularia coreana snail. These data also indicate that N. risticii could be widespread in Korea.
Bae Keun Park - One of the best experts on this subject based on the ideXlab platform.
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Identification of trematode cercariae carrying Neorickettsia risticii in freshwater stream snails.
Annals of the New York Academy of Sciences, 2003Co-Authors: Bae Keun Park, Meen‐ju Kim, Eun-ha Kim, Min-seok Kim, Joon-seok ChaeAbstract:: We provide evidence of Neorickettsia (Ehrlichia) risticii Holland, the agent of Potomac horse fever, in trematode larval stages found in aquatic snails and insects collected from a stream in Korea, using nested polymerase chain reaction (PCR) and sequence analysis of the 16S rRNA gene fragment amplified from several cercaria species. It was observed that of 423 (13.1%) of 3,219 snails infected with cercariae, 77 (20.8% of the 371) were infected with N. risticii. Five families of trematode cercariae, Schistosomatidae, Echimostomatidae, Heterophyidae, Microphallidae, and Acanthocopidae were identified morphologically within Semisulcospira libertina, Radix auricularia coreana, and S. gottschei snails. Echinostoma cinetorchis, E. hortense, and Metagonimus sp. were identified based on both the cercarial morphology as well as by phylogenetic analysis of the amplified 18S rRNA gene sequences. Adult aquatic insects were also collected from the same sites and were sorted into five species, Ischnura asiatica in Coenagrionidae and Calopteryx japonica, Sympetrum darwinianum, Symptrum eroticum, and Symptrum parvulum in Calopterygoidae. One thousand and two hundred eighty five metacercariae (classified into groups A through F) were isolated from 310 adult aquatic insects, and the average number of metacercariae per aquatic insect was 4.1. However, there was no amplification of N. risticii from these metacercariae.
