The Experts below are selected from a list of 30 Experts worldwide ranked by ideXlab platform
Gyula Szegedi - One of the best experts on this subject based on the ideXlab platform.
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significant correlation between the cd63 assay and the histamine release assay in chronic urticaria
British Journal of Dermatology, 2006Co-Authors: Andrea Szegedi, Beatrix Irinyi, M Gal, J Hunyadi, Katalin Danko, Emese Kiss, Sandor Sipka, Gyula SzegediAbstract:Summary Background Antibodies directed to the α subunit of the high affinity IgE receptor and the IgE molecule are proposed to be of pathogenetic relevance in a group of patients with chronic urticaria (CU). The diagnosis of autoimmune chronic urticaria (ACU) is difficult; the autologous serum skin test (ASST) seems to be a useful screening test, but reliable, additional confirmatory methods are needed. Objectives To assess the diagnostic value of a modified serum-induced basophil activation test, the CD63 expression assay, in the diagnosis of ACU by comparing the results of the CD63 assay with the results of the histamine release (HR) test, the ASST and serum levels of soluble CD40 ligand (sCD40L). Methods Using basophils from an atopic (DA) and a nonatopic (DNA) Donor the activity of sera of 72 patients with CU were measured in HR assay by enzyme-linked immunosorbent assay and in CD63 expression assay by flow cytometry. An ASST was carried out in all patients; in 30 of the 72 patients sCD40L was detected and correlations were derived between the different assays. Sera of 20 normal controls and 26 patients with systemic autoimmune diseases were also tested in the HR assay and in the CD63 expression assay. Results Histamine-releasing activity was detected in the sera of 51% (DA) and 32% (DNA) of CU patients and 57% (DA) and 28% (DNA) of sera upregulated CD63 expression on the surface of basophils from the different Donors. There was a significant correlation between the HR and the CD63 assays carried out on both Donors, but the ASST showed a strong correlation with the HR assay only for basophils from the DA. The serum level of sCD40L was significantly higher in patients with CU compared with controls, but the difference between the autoimmune and the nonautoimmune groups was not significant. Conclusions The CD63 expression assay seems to be a reliable functional test in the diagnosis of ACU, particularly if highly Sensitive Donor basophils are used, but the determination of the sCD40L serum level was not sufficient to differentiate between the autoimmune and the nonautoimmune patient groups.
Sandor Sipka - One of the best experts on this subject based on the ideXlab platform.
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significant correlation between the cd63 assay and the histamine release assay in chronic urticaria
British Journal of Dermatology, 2006Co-Authors: Andrea Szegedi, Beatrix Irinyi, M Gal, J Hunyadi, Katalin Danko, Emese Kiss, Sandor Sipka, Gyula SzegediAbstract:Summary Background Antibodies directed to the α subunit of the high affinity IgE receptor and the IgE molecule are proposed to be of pathogenetic relevance in a group of patients with chronic urticaria (CU). The diagnosis of autoimmune chronic urticaria (ACU) is difficult; the autologous serum skin test (ASST) seems to be a useful screening test, but reliable, additional confirmatory methods are needed. Objectives To assess the diagnostic value of a modified serum-induced basophil activation test, the CD63 expression assay, in the diagnosis of ACU by comparing the results of the CD63 assay with the results of the histamine release (HR) test, the ASST and serum levels of soluble CD40 ligand (sCD40L). Methods Using basophils from an atopic (DA) and a nonatopic (DNA) Donor the activity of sera of 72 patients with CU were measured in HR assay by enzyme-linked immunosorbent assay and in CD63 expression assay by flow cytometry. An ASST was carried out in all patients; in 30 of the 72 patients sCD40L was detected and correlations were derived between the different assays. Sera of 20 normal controls and 26 patients with systemic autoimmune diseases were also tested in the HR assay and in the CD63 expression assay. Results Histamine-releasing activity was detected in the sera of 51% (DA) and 32% (DNA) of CU patients and 57% (DA) and 28% (DNA) of sera upregulated CD63 expression on the surface of basophils from the different Donors. There was a significant correlation between the HR and the CD63 assays carried out on both Donors, but the ASST showed a strong correlation with the HR assay only for basophils from the DA. The serum level of sCD40L was significantly higher in patients with CU compared with controls, but the difference between the autoimmune and the nonautoimmune groups was not significant. Conclusions The CD63 expression assay seems to be a reliable functional test in the diagnosis of ACU, particularly if highly Sensitive Donor basophils are used, but the determination of the sCD40L serum level was not sufficient to differentiate between the autoimmune and the nonautoimmune patient groups.
Decheng Wan - One of the best experts on this subject based on the ideXlab platform.
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molecular engineering of uv vis light emitting diode led Sensitive Donor π acceptor type sulfonium salt photoacid generators design synthesis and study of photochemical and photophysical properties
Chemistry: A European Journal, 2017Co-Authors: Ming Jin, Jianchao Xie, Jeanpierre Malval, Decheng WanAbstract:A series of Donor-π-acceptor-type sulfonium salt photoacid generators (PAGs) were designed and synthesized by systematically changing electron-donating groups, π-conjugated systems, electron-withdrawing groups, and the number of branches through molecular engineering. These PAGs can effectively decompose under UV/Vis irradiation from a light-emitting diode (LED) light source because of the matching absorption and emitting spectra of the LEDs. The absorption and acid-generation properties of these sulfonium salts were elucidated by UV/Vis spectroscopy and so forth. Results indicated that the PAG performance benefited from the introduction of strong electron-donating groups, specific π-conjugated structures, certain electron-withdrawing groups, or two-branched structures. Most sulfonium salts showed potential as photoinitiators under irradiation by a wide variety of UV and visible LEDs.
Hitoshi Miyasaka - One of the best experts on this subject based on the ideXlab platform.
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a charge disproportionate ordered state with δ 0 75 in a chemically Sensitive Donor acceptor dδ 2a2δ layered framework
Chemical Communications, 2015Co-Authors: Hiroki Fukunaga, Takafumi Yoshino, Hajime Sagayama, Junichi Yamaura, T Arima, Wataru Kosaka, Hitoshi MiyasakaAbstract:A novel charge-disproportionation state with δ = 0.75 was observed in an electron-Donor (D)/-acceptor (A) Dδ+2A2δ− layered framework by chemically tuning the electron-donating affinity of D at the boundary between D0.5+2A− and D+2A2− phases, which was pressure-Sensitive due to the formation of the D+2A2− oxidation state.
Andrea Szegedi - One of the best experts on this subject based on the ideXlab platform.
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significant correlation between the cd63 assay and the histamine release assay in chronic urticaria
British Journal of Dermatology, 2006Co-Authors: Andrea Szegedi, Beatrix Irinyi, M Gal, J Hunyadi, Katalin Danko, Emese Kiss, Sandor Sipka, Gyula SzegediAbstract:Summary Background Antibodies directed to the α subunit of the high affinity IgE receptor and the IgE molecule are proposed to be of pathogenetic relevance in a group of patients with chronic urticaria (CU). The diagnosis of autoimmune chronic urticaria (ACU) is difficult; the autologous serum skin test (ASST) seems to be a useful screening test, but reliable, additional confirmatory methods are needed. Objectives To assess the diagnostic value of a modified serum-induced basophil activation test, the CD63 expression assay, in the diagnosis of ACU by comparing the results of the CD63 assay with the results of the histamine release (HR) test, the ASST and serum levels of soluble CD40 ligand (sCD40L). Methods Using basophils from an atopic (DA) and a nonatopic (DNA) Donor the activity of sera of 72 patients with CU were measured in HR assay by enzyme-linked immunosorbent assay and in CD63 expression assay by flow cytometry. An ASST was carried out in all patients; in 30 of the 72 patients sCD40L was detected and correlations were derived between the different assays. Sera of 20 normal controls and 26 patients with systemic autoimmune diseases were also tested in the HR assay and in the CD63 expression assay. Results Histamine-releasing activity was detected in the sera of 51% (DA) and 32% (DNA) of CU patients and 57% (DA) and 28% (DNA) of sera upregulated CD63 expression on the surface of basophils from the different Donors. There was a significant correlation between the HR and the CD63 assays carried out on both Donors, but the ASST showed a strong correlation with the HR assay only for basophils from the DA. The serum level of sCD40L was significantly higher in patients with CU compared with controls, but the difference between the autoimmune and the nonautoimmune groups was not significant. Conclusions The CD63 expression assay seems to be a reliable functional test in the diagnosis of ACU, particularly if highly Sensitive Donor basophils are used, but the determination of the sCD40L serum level was not sufficient to differentiate between the autoimmune and the nonautoimmune patient groups.
