pig and human Sensitized Lymphocyte co culture and cytokines measurement

Objective:To investigate the immune response mechanism involved in clinical transplantion of xenogeneic acellular dermal matrix(Xeno-ADM).Methods:Peripheric blood mononuclear cells(PBMC)were separated respectively from normal pigs and humans,and patients suffered extensive burn accepted the transplantation of Xeno-ADM.Then the mixed Lymphocyte cultrue(MLC)was performed through co culturing PBMC of the patients with PBMC of the normal pigs or humans.The cells proliferation,and secretion of IL-2(IU·ml -1 ),IL-4(IU·ml -1 )and IFN-(ng·L -1 )were measured by 3 H-TdR incorporation and enzyme linked immunosorbent assay(ELISA).Results:At 4 days after culture,the cells proliferation and secretion of IL-2,IL-4,IFN  were significantly higher in MLC of normal pigs and patients than in that of normal humans and patients( P 0.05～0.01).Conclusion:Th1 and Th2-mediated immunity and IL-4 induced humoral immunity might be the characteristic of immunologic rejection to Xeno-ADM.MLC with Sensitized Lymphocyte may reflect the in vivo immunoreactivity more accurately.