The Experts below are selected from a list of 6 Experts worldwide ranked by ideXlab platform
Hianik T. - One of the best experts on this subject based on the ideXlab platform.
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Layer-by-layer polyelectrolyte assembles involving DNA as a platform for DNA Sensors
2020Co-Authors: Evtugy G., Hianik T.Abstract:The Development of DNA-Sensors has become significantly important in the past decades due to prospects of application in medicine, biotechnology and exploring fundamental problems related to cell biology and DNA functioning. Layer-by-layer (LbL) immobilization provides unique approach to the implementation of DNA into the surface sensing layers, a crucial step of DNA-Sensor Development. The review considers main aspects of LbL assembling in DNA-Sensor Development and application for the detection of complementary oligonucleotides and DNA damage assessment. Besides, electrostatic assembling due to stepwise accumulation of oppositely charged layers, various combinations of covalent binding and affine immobilization are also considered. The characteristics of DNA containing multilayers onto the solid support and the effect of the immobilization techniques and layers assembled on the performance of appropriate DNASensors are summarized for different target analytes. © 2011 Bentham Science Publishers Ltd
Evtugy G. - One of the best experts on this subject based on the ideXlab platform.
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Layer-by-layer polyelectrolyte assembles involving DNA as a platform for DNA Sensors
2020Co-Authors: Evtugy G., Hianik T.Abstract:The Development of DNA-Sensors has become significantly important in the past decades due to prospects of application in medicine, biotechnology and exploring fundamental problems related to cell biology and DNA functioning. Layer-by-layer (LbL) immobilization provides unique approach to the implementation of DNA into the surface sensing layers, a crucial step of DNA-Sensor Development. The review considers main aspects of LbL assembling in DNA-Sensor Development and application for the detection of complementary oligonucleotides and DNA damage assessment. Besides, electrostatic assembling due to stepwise accumulation of oppositely charged layers, various combinations of covalent binding and affine immobilization are also considered. The characteristics of DNA containing multilayers onto the solid support and the effect of the immobilization techniques and layers assembled on the performance of appropriate DNASensors are summarized for different target analytes. © 2011 Bentham Science Publishers Ltd
Zamora Féli - One of the best experts on this subject based on the ideXlab platform.
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Functionalization of a Few-Layer Antimonene with Oligonucleotides for DNA Sensing
'American Chemical Society (ACS)', 2021Co-Authors: García-mendiola Tania, Gutiérrez-sánchez Cristina, Gibaja Carlos, Torres Iñigo, Usó-rogero Carlos, Pariente Féli, Solera Jesús, Razavifa Zahra, Palacios, Jua J., Zamora FéliAbstract:This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Nano Materials, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see: https://pubs.acs.org/doi/abs/10.1021/acsanm.0c00335Antimonene, a novel group 15 two-dimensional material, is functionalized with an oligonucleotide as a first step to DNA Sensor Development. The functionalization process leads to a few-layer antimonene modified with DNA that after deposition on gold screen-printed electrodes gives a simple and efficient DNA electrochemical sensing platform. We provide theoretical and experimental data of the DNA–antimonene interaction, confirming that oligonucleotides interact noncovalently but strongly with antimonene. The potential utility of this antimonene-based sensing device is assessed using, as a case of study, a sequence from the BRCA1 gene as the target DNA. The selectivity of the device allows not only recognition of a specific DNA sequence but also detection of a mutation in this gene associated with breast cancer, directly in clinical samplesThe Ministerio de Ciencia Innovación y Universidades (Grants CTQ2017-84309-C2-1-R, MAT2016-77608-C3-1-P, PCI2018-093081, JTC2017/2D-Sb&Ge, and FIS2016-80434-P), Generalitat Valenciana (Grant APOSTD/2017/010), and CAM (Grants TransNANOAVANSENS and 2017-T1/BIO-5435) are gratefully acknowledged. We also acknowledge the María de Maeztu Programme for Units of Excellence in R&D (MDM-2014-0377), the Fundación Ramón Areces, and the computer resources and assistance provided by the Centro de Computación Científica of the Universidad Autónoma de Madri
García-mendiola Tania - One of the best experts on this subject based on the ideXlab platform.
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Functionalization of a Few-Layer Antimonene with Oligonucleotides for DNA Sensing
'American Chemical Society (ACS)', 2021Co-Authors: García-mendiola Tania, Gutiérrez-sánchez Cristina, Gibaja Carlos, Torres Iñigo, Usó-rogero Carlos, Pariente Féli, Solera Jesús, Razavifa Zahra, Palacios, Jua J., Zamora FéliAbstract:This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Nano Materials, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see: https://pubs.acs.org/doi/abs/10.1021/acsanm.0c00335Antimonene, a novel group 15 two-dimensional material, is functionalized with an oligonucleotide as a first step to DNA Sensor Development. The functionalization process leads to a few-layer antimonene modified with DNA that after deposition on gold screen-printed electrodes gives a simple and efficient DNA electrochemical sensing platform. We provide theoretical and experimental data of the DNA–antimonene interaction, confirming that oligonucleotides interact noncovalently but strongly with antimonene. The potential utility of this antimonene-based sensing device is assessed using, as a case of study, a sequence from the BRCA1 gene as the target DNA. The selectivity of the device allows not only recognition of a specific DNA sequence but also detection of a mutation in this gene associated with breast cancer, directly in clinical samplesThe Ministerio de Ciencia Innovación y Universidades (Grants CTQ2017-84309-C2-1-R, MAT2016-77608-C3-1-P, PCI2018-093081, JTC2017/2D-Sb&Ge, and FIS2016-80434-P), Generalitat Valenciana (Grant APOSTD/2017/010), and CAM (Grants TransNANOAVANSENS and 2017-T1/BIO-5435) are gratefully acknowledged. We also acknowledge the María de Maeztu Programme for Units of Excellence in R&D (MDM-2014-0377), the Fundación Ramón Areces, and the computer resources and assistance provided by the Centro de Computación Científica of the Universidad Autónoma de Madri
Gutiérrez-sánchez Cristina - One of the best experts on this subject based on the ideXlab platform.
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Functionalization of a Few-Layer Antimonene with Oligonucleotides for DNA Sensing
'American Chemical Society (ACS)', 2021Co-Authors: García-mendiola Tania, Gutiérrez-sánchez Cristina, Gibaja Carlos, Torres Iñigo, Usó-rogero Carlos, Pariente Féli, Solera Jesús, Razavifa Zahra, Palacios, Jua J., Zamora FéliAbstract:This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Nano Materials, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see: https://pubs.acs.org/doi/abs/10.1021/acsanm.0c00335Antimonene, a novel group 15 two-dimensional material, is functionalized with an oligonucleotide as a first step to DNA Sensor Development. The functionalization process leads to a few-layer antimonene modified with DNA that after deposition on gold screen-printed electrodes gives a simple and efficient DNA electrochemical sensing platform. We provide theoretical and experimental data of the DNA–antimonene interaction, confirming that oligonucleotides interact noncovalently but strongly with antimonene. The potential utility of this antimonene-based sensing device is assessed using, as a case of study, a sequence from the BRCA1 gene as the target DNA. The selectivity of the device allows not only recognition of a specific DNA sequence but also detection of a mutation in this gene associated with breast cancer, directly in clinical samplesThe Ministerio de Ciencia Innovación y Universidades (Grants CTQ2017-84309-C2-1-R, MAT2016-77608-C3-1-P, PCI2018-093081, JTC2017/2D-Sb&Ge, and FIS2016-80434-P), Generalitat Valenciana (Grant APOSTD/2017/010), and CAM (Grants TransNANOAVANSENS and 2017-T1/BIO-5435) are gratefully acknowledged. We also acknowledge the María de Maeztu Programme for Units of Excellence in R&D (MDM-2014-0377), the Fundación Ramón Areces, and the computer resources and assistance provided by the Centro de Computación Científica of the Universidad Autónoma de Madri
