The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
V. M. Mikhailovich - One of the best experts on this subject based on the ideXlab platform.
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Separate Production of single-stranded DNA is not necessary: circuit denaturation of double-stranded DNA followed by hybridization of single strands on oligonucleotide microchips.
Journal of biomolecular structure & dynamics, 2009Co-Authors: Vadim A. Vasiliskov, Alexander V. Chudinov, V. R. Chechetkin, S. A. Surzhikov, Alexander S. Zasedatelev, V. M. MikhailovichAbstract:An approach to circuit renaturation-hybridization of dsDNA on oligonucleotide microchips is described. A close circuit cycling device has been developed, and the feasibility of the proposed technique was demonstrated on two platforms. First, a commercial microchip for detection of rifampicin resistance in Mycobacterium tuberculosis was used. Hybridization of a 126 nt long single-stranded DNA (ssDNA) fragment of the rpoB gene according to manufacturer's protocol has been compared to hybridization of the same double-stranded DNA (dsDNA) fragment using the developed approach. Hybridization signals obtained by both methods were comparable in intensity and correlated closely. Second, a 22 nt long hairpin-forming oligonucleotide was designed and hybridized with a custom microchip containing probes complementary to both strands of the oligonucleotide. Conventional hybridization of this oligonucleotide did not yield any significant signals. Cleavage of the hairpin loop resulted in the formation of a 9 bp long intermolecular duplex. Hybridization of the duplex using the suggested technique yielded strong signals. The proposed approach allows analyzing target DNA in double-stranded form bypassing the preparation of single-stranded targets. Moreover, both complementary chains could be analyzed simultaneously, providing a reliable internal control. Being combined with fragmentation this method opens new possibilities in analyzing ssDNA with complex secondary structure.
D. Russolillo - One of the best experts on this subject based on the ideXlab platform.
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combined vs Separate heat and power Production primary energy comparison in high renewable share contexts
Applied Energy, 2018Co-Authors: Michel Noussan, Matteo Jarre, R. Roberto, D. RussolilloAbstract:Abstract Natural Gas Combined Cycle (NGCC) units are currently the most efficient power plants based on fossil fuels. When used for Combined Heat and Power (CHP) Production, serving District Heating (DH) systems, they have been usually promoted by stating their lower primary energy consumption compared to Separate Production of power and heat with conventional technologies. However, a significant increase of the share of Renewable Energy Sources (RES) in power generation and Heat Pumps (HP) for heat Production in buildings could undermine this assumption. This paper evaluates a case study in Northern Italy, by comparing the real operation of three NGCC plants serving a DH network against the Separate Production of power (from real data of the National electricity mix) and heat (considering two scenarios based on natural gas boilers and heat pumps). The analysis is performed on hourly data over a two-years’ time frame, to highlight the variations across the hours of the day and the seasons. To perform a comprehensive analysis, the entire system performance is considered, by comparing the useful energy supplied to the users to the primary energy consumption. The results show how the primary energy savings of fossil CHP technologies are strongly related with the available alternatives, which have been going through a significant evolution in last years. The Separate Production of heat and power can now be performed with competitive technologies, which benefit from the high share of RES in electricity Production. Therefore, the comparison between combined and Separate Production is influenced by the high variability of the electricity generation mix, which needs to be carefully considered.
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Combined vs Separate heat and power Production – Primary energy comparison in high renewable share contexts
Applied Energy, 2018Co-Authors: Michel Noussan, Matteo Jarre, R. Roberto, D. RussolilloAbstract:Abstract Natural Gas Combined Cycle (NGCC) units are currently the most efficient power plants based on fossil fuels. When used for Combined Heat and Power (CHP) Production, serving District Heating (DH) systems, they have been usually promoted by stating their lower primary energy consumption compared to Separate Production of power and heat with conventional technologies. However, a significant increase of the share of Renewable Energy Sources (RES) in power generation and Heat Pumps (HP) for heat Production in buildings could undermine this assumption. This paper evaluates a case study in Northern Italy, by comparing the real operation of three NGCC plants serving a DH network against the Separate Production of power (from real data of the National electricity mix) and heat (considering two scenarios based on natural gas boilers and heat pumps). The analysis is performed on hourly data over a two-years’ time frame, to highlight the variations across the hours of the day and the seasons. To perform a comprehensive analysis, the entire system performance is considered, by comparing the useful energy supplied to the users to the primary energy consumption. The results show how the primary energy savings of fossil CHP technologies are strongly related with the available alternatives, which have been going through a significant evolution in last years. The Separate Production of heat and power can now be performed with competitive technologies, which benefit from the high share of RES in electricity Production. Therefore, the comparison between combined and Separate Production is influenced by the high variability of the electricity generation mix, which needs to be carefully considered.
Vadim A. Vasiliskov - One of the best experts on this subject based on the ideXlab platform.
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Separate Production of single-stranded DNA is not necessary: circuit denaturation of double-stranded DNA followed by hybridization of single strands on oligonucleotide microchips.
Journal of biomolecular structure & dynamics, 2009Co-Authors: Vadim A. Vasiliskov, Alexander V. Chudinov, V. R. Chechetkin, S. A. Surzhikov, Alexander S. Zasedatelev, V. M. MikhailovichAbstract:An approach to circuit renaturation-hybridization of dsDNA on oligonucleotide microchips is described. A close circuit cycling device has been developed, and the feasibility of the proposed technique was demonstrated on two platforms. First, a commercial microchip for detection of rifampicin resistance in Mycobacterium tuberculosis was used. Hybridization of a 126 nt long single-stranded DNA (ssDNA) fragment of the rpoB gene according to manufacturer's protocol has been compared to hybridization of the same double-stranded DNA (dsDNA) fragment using the developed approach. Hybridization signals obtained by both methods were comparable in intensity and correlated closely. Second, a 22 nt long hairpin-forming oligonucleotide was designed and hybridized with a custom microchip containing probes complementary to both strands of the oligonucleotide. Conventional hybridization of this oligonucleotide did not yield any significant signals. Cleavage of the hairpin loop resulted in the formation of a 9 bp long intermolecular duplex. Hybridization of the duplex using the suggested technique yielded strong signals. The proposed approach allows analyzing target DNA in double-stranded form bypassing the preparation of single-stranded targets. Moreover, both complementary chains could be analyzed simultaneously, providing a reliable internal control. Being combined with fragmentation this method opens new possibilities in analyzing ssDNA with complex secondary structure.
Michel Noussan - One of the best experts on this subject based on the ideXlab platform.
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combined vs Separate heat and power Production primary energy comparison in high renewable share contexts
Applied Energy, 2018Co-Authors: Michel Noussan, Matteo Jarre, R. Roberto, D. RussolilloAbstract:Abstract Natural Gas Combined Cycle (NGCC) units are currently the most efficient power plants based on fossil fuels. When used for Combined Heat and Power (CHP) Production, serving District Heating (DH) systems, they have been usually promoted by stating their lower primary energy consumption compared to Separate Production of power and heat with conventional technologies. However, a significant increase of the share of Renewable Energy Sources (RES) in power generation and Heat Pumps (HP) for heat Production in buildings could undermine this assumption. This paper evaluates a case study in Northern Italy, by comparing the real operation of three NGCC plants serving a DH network against the Separate Production of power (from real data of the National electricity mix) and heat (considering two scenarios based on natural gas boilers and heat pumps). The analysis is performed on hourly data over a two-years’ time frame, to highlight the variations across the hours of the day and the seasons. To perform a comprehensive analysis, the entire system performance is considered, by comparing the useful energy supplied to the users to the primary energy consumption. The results show how the primary energy savings of fossil CHP technologies are strongly related with the available alternatives, which have been going through a significant evolution in last years. The Separate Production of heat and power can now be performed with competitive technologies, which benefit from the high share of RES in electricity Production. Therefore, the comparison between combined and Separate Production is influenced by the high variability of the electricity generation mix, which needs to be carefully considered.
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Combined vs Separate heat and power Production – Primary energy comparison in high renewable share contexts
Applied Energy, 2018Co-Authors: Michel Noussan, Matteo Jarre, R. Roberto, D. RussolilloAbstract:Abstract Natural Gas Combined Cycle (NGCC) units are currently the most efficient power plants based on fossil fuels. When used for Combined Heat and Power (CHP) Production, serving District Heating (DH) systems, they have been usually promoted by stating their lower primary energy consumption compared to Separate Production of power and heat with conventional technologies. However, a significant increase of the share of Renewable Energy Sources (RES) in power generation and Heat Pumps (HP) for heat Production in buildings could undermine this assumption. This paper evaluates a case study in Northern Italy, by comparing the real operation of three NGCC plants serving a DH network against the Separate Production of power (from real data of the National electricity mix) and heat (considering two scenarios based on natural gas boilers and heat pumps). The analysis is performed on hourly data over a two-years’ time frame, to highlight the variations across the hours of the day and the seasons. To perform a comprehensive analysis, the entire system performance is considered, by comparing the useful energy supplied to the users to the primary energy consumption. The results show how the primary energy savings of fossil CHP technologies are strongly related with the available alternatives, which have been going through a significant evolution in last years. The Separate Production of heat and power can now be performed with competitive technologies, which benefit from the high share of RES in electricity Production. Therefore, the comparison between combined and Separate Production is influenced by the high variability of the electricity generation mix, which needs to be carefully considered.
Pier Ruggero Spina - One of the best experts on this subject based on the ideXlab platform.
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Guidelines for residential micro-CHP systems design
Applied Energy, 2012Co-Authors: M Bianchi, Andrea De Pascale, Pier Ruggero SpinaAbstract:The aim of this paper is to provide general guidelines for the design of micro-CHP systems for the heating of residential buildings. A micro-CHP system is intended as a system composed of a prime mover, a thermal storage system and an auxiliary boiler. In particular, the analyses carried out in the paper provide guidelines to select the proper prime mover technology and size and thermal storage system size, with reference to prime mover operating hours and produced electric and thermal energy. Moreover, both primary energy saving and profitability of the CHP system compared to the Separate Production of electricity and heat are also evaluated.
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Best practice in residential micro-CHP systems design
Umberto Desideri Jinyue Yan, 2011Co-Authors: M Bianchi, Andrea De Pascale, Pier Ruggero SpinaAbstract:The aim of this paper is to evaluate the profitability of micro-CHP systems for the heating of residential buildings. A micro-CHP system is intended as a system composed of a prime mover, a thermal storage system and an auxiliary boiler. For this kind of system analyses were carried out in order to evaluate both profitability and primary energy saving compared to the Separate Production of electricity and heat. In particular, the analyses provide the operating hours, the produced electric and thermal energy and the allowable marginal cost of the CHP system with respect to a traditional boiler as a function of prime mover technology and size and of the thermal storage system size. Moreover, the relation between the CHP Production (operating hours) and the thermal storage system size is evaluated thoroughly, showing its effect on the economic results
