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Yan Zhou - One of the best experts on this subject based on the ideXlab platform.
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study on the Separation Mechanism of solid substrate electrospray ionization mass spectrometry
IEEE Journal of Solid-state Circuits, 2021Co-Authors: Peiyu Shi, Bing Xia, Yan ZhouAbstract:Solid-substrate electrospray ionization mass spectrometry is an important ambient ionization technology to simplify mass spectrometry analysis. Nowadays, its Separation application has been reported increasingly, however, the detailed Separation Mechanism is still indistinct although the chromatographic effect was reported as a possible factor. In this study, substrate-filled capillary electrospray ionization mass spectrometry was developed as an ideal model to investigate the Separation Mechanism using over thirty small molecules (neutral, basic, and weakly acidic) as model compounds with C18-bonded silica gel and silica gel as the substrates. The chromatographic effect was validated by the negative t-value of oil-water distribution coefficient, and the electric field effect was verified by the paired t-test (p < 0.01) between the retention times at 5.5 and 4.0 kV. A differential equation was proposed to quantify the compound retention under electric field. The quantitative method was validated to rapidly quantify proline (31.88 μg/mL) and hydroxyproline (20.71 μg/mL) in plasma with acceptable selectivity and accuracy. In conclusion, the Separation Mechanism of solid-substrate electrospray ionization mass spectrometry was the combination of the chromatographic and electric field effects, which could provide theoretical guidance for the Separation optimization, and also promote its applications in biological, pharmaceutical, forensic, food and environmental analyses, etc.
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Study on the Separation Mechanism of solid‐substrate electrospray ionization mass spectrometry
Journal of separation science, 2020Co-Authors: Peiyu Shi, Bing Xia, Yan ZhouAbstract:Solid-substrate electrospray ionization MS is an important ambient ionization technology to simplify MS analysis. Nowadays, its Separation application has been reported increasingly, however, the detailed Separation Mechanism is still indistinct although the chromatographic effect was reported as a possible factor. In this study, substrate-filled capillary electrospray ionization MS was developed as an ideal model to investigate the Separation Mechanism using over thirty small molecules (neutral, basic, and weakly acidic) as model compounds with C18-bonded silica gel and silica gel as the substrates. The chromatographic effect was validated by the negative t-value of oil-water distribution coefficient, and the electric field effect was verified by the paired t-test (p < 0.01) between the retention times at 5.5 kV and 4.0 kV. A differential equation was proposed to quantify the compound retention under electric field. The quantitative method was validated to rapidly quantify proline (31.88 μg/mL) and hydroxyproline (20.71 μg/mL) in plasma with acceptable selectivity and accuracy. In conclusion, the Separation Mechanism of solid-substrate electrospray ionization MS was the combination of the chromatographic and electric field effects, which could provide theoretical guidance for the Separation optimization, and also promote its applications in biological, pharmaceutical, forensic, food and environmental analyses, etc. This article is protected by copyright. All rights reserved.
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Study on the Separation Mechanism of solid‐substrate electrospray ionization mass spectrometry
Journal of separation science, 2020Co-Authors: Peiyu Shi, Bing Xia, Yan ZhouAbstract:Solid-substrate electrospray ionization mass spectrometry is an important ambient ionization technology to simplify mass spectrometry analysis. Nowadays, its Separation application has been reported increasingly, however, the detailed Separation Mechanism is still indistinct although the chromatographic effect was reported as a possible factor. In this study, substrate-filled capillary electrospray ionization mass spectrometry was developed as an ideal model to investigate the Separation Mechanism using over thirty small molecules (neutral, basic, and weakly acidic) as model compounds with C18-bonded silica gel and silica gel as the substrates. The chromatographic effect was validated by the negative t-value of oil-water distribution coefficient, and the electric field effect was verified by the paired t-test (p
Peiyu Shi - One of the best experts on this subject based on the ideXlab platform.
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study on the Separation Mechanism of solid substrate electrospray ionization mass spectrometry
IEEE Journal of Solid-state Circuits, 2021Co-Authors: Peiyu Shi, Bing Xia, Yan ZhouAbstract:Solid-substrate electrospray ionization mass spectrometry is an important ambient ionization technology to simplify mass spectrometry analysis. Nowadays, its Separation application has been reported increasingly, however, the detailed Separation Mechanism is still indistinct although the chromatographic effect was reported as a possible factor. In this study, substrate-filled capillary electrospray ionization mass spectrometry was developed as an ideal model to investigate the Separation Mechanism using over thirty small molecules (neutral, basic, and weakly acidic) as model compounds with C18-bonded silica gel and silica gel as the substrates. The chromatographic effect was validated by the negative t-value of oil-water distribution coefficient, and the electric field effect was verified by the paired t-test (p < 0.01) between the retention times at 5.5 and 4.0 kV. A differential equation was proposed to quantify the compound retention under electric field. The quantitative method was validated to rapidly quantify proline (31.88 μg/mL) and hydroxyproline (20.71 μg/mL) in plasma with acceptable selectivity and accuracy. In conclusion, the Separation Mechanism of solid-substrate electrospray ionization mass spectrometry was the combination of the chromatographic and electric field effects, which could provide theoretical guidance for the Separation optimization, and also promote its applications in biological, pharmaceutical, forensic, food and environmental analyses, etc.
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Study on the Separation Mechanism of solid‐substrate electrospray ionization mass spectrometry
Journal of separation science, 2020Co-Authors: Peiyu Shi, Bing Xia, Yan ZhouAbstract:Solid-substrate electrospray ionization MS is an important ambient ionization technology to simplify MS analysis. Nowadays, its Separation application has been reported increasingly, however, the detailed Separation Mechanism is still indistinct although the chromatographic effect was reported as a possible factor. In this study, substrate-filled capillary electrospray ionization MS was developed as an ideal model to investigate the Separation Mechanism using over thirty small molecules (neutral, basic, and weakly acidic) as model compounds with C18-bonded silica gel and silica gel as the substrates. The chromatographic effect was validated by the negative t-value of oil-water distribution coefficient, and the electric field effect was verified by the paired t-test (p < 0.01) between the retention times at 5.5 kV and 4.0 kV. A differential equation was proposed to quantify the compound retention under electric field. The quantitative method was validated to rapidly quantify proline (31.88 μg/mL) and hydroxyproline (20.71 μg/mL) in plasma with acceptable selectivity and accuracy. In conclusion, the Separation Mechanism of solid-substrate electrospray ionization MS was the combination of the chromatographic and electric field effects, which could provide theoretical guidance for the Separation optimization, and also promote its applications in biological, pharmaceutical, forensic, food and environmental analyses, etc. This article is protected by copyright. All rights reserved.
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Study on the Separation Mechanism of solid‐substrate electrospray ionization mass spectrometry
Journal of separation science, 2020Co-Authors: Peiyu Shi, Bing Xia, Yan ZhouAbstract:Solid-substrate electrospray ionization mass spectrometry is an important ambient ionization technology to simplify mass spectrometry analysis. Nowadays, its Separation application has been reported increasingly, however, the detailed Separation Mechanism is still indistinct although the chromatographic effect was reported as a possible factor. In this study, substrate-filled capillary electrospray ionization mass spectrometry was developed as an ideal model to investigate the Separation Mechanism using over thirty small molecules (neutral, basic, and weakly acidic) as model compounds with C18-bonded silica gel and silica gel as the substrates. The chromatographic effect was validated by the negative t-value of oil-water distribution coefficient, and the electric field effect was verified by the paired t-test (p
Bing Xia - One of the best experts on this subject based on the ideXlab platform.
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study on the Separation Mechanism of solid substrate electrospray ionization mass spectrometry
IEEE Journal of Solid-state Circuits, 2021Co-Authors: Peiyu Shi, Bing Xia, Yan ZhouAbstract:Solid-substrate electrospray ionization mass spectrometry is an important ambient ionization technology to simplify mass spectrometry analysis. Nowadays, its Separation application has been reported increasingly, however, the detailed Separation Mechanism is still indistinct although the chromatographic effect was reported as a possible factor. In this study, substrate-filled capillary electrospray ionization mass spectrometry was developed as an ideal model to investigate the Separation Mechanism using over thirty small molecules (neutral, basic, and weakly acidic) as model compounds with C18-bonded silica gel and silica gel as the substrates. The chromatographic effect was validated by the negative t-value of oil-water distribution coefficient, and the electric field effect was verified by the paired t-test (p < 0.01) between the retention times at 5.5 and 4.0 kV. A differential equation was proposed to quantify the compound retention under electric field. The quantitative method was validated to rapidly quantify proline (31.88 μg/mL) and hydroxyproline (20.71 μg/mL) in plasma with acceptable selectivity and accuracy. In conclusion, the Separation Mechanism of solid-substrate electrospray ionization mass spectrometry was the combination of the chromatographic and electric field effects, which could provide theoretical guidance for the Separation optimization, and also promote its applications in biological, pharmaceutical, forensic, food and environmental analyses, etc.
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Study on the Separation Mechanism of solid‐substrate electrospray ionization mass spectrometry
Journal of separation science, 2020Co-Authors: Peiyu Shi, Bing Xia, Yan ZhouAbstract:Solid-substrate electrospray ionization MS is an important ambient ionization technology to simplify MS analysis. Nowadays, its Separation application has been reported increasingly, however, the detailed Separation Mechanism is still indistinct although the chromatographic effect was reported as a possible factor. In this study, substrate-filled capillary electrospray ionization MS was developed as an ideal model to investigate the Separation Mechanism using over thirty small molecules (neutral, basic, and weakly acidic) as model compounds with C18-bonded silica gel and silica gel as the substrates. The chromatographic effect was validated by the negative t-value of oil-water distribution coefficient, and the electric field effect was verified by the paired t-test (p < 0.01) between the retention times at 5.5 kV and 4.0 kV. A differential equation was proposed to quantify the compound retention under electric field. The quantitative method was validated to rapidly quantify proline (31.88 μg/mL) and hydroxyproline (20.71 μg/mL) in plasma with acceptable selectivity and accuracy. In conclusion, the Separation Mechanism of solid-substrate electrospray ionization MS was the combination of the chromatographic and electric field effects, which could provide theoretical guidance for the Separation optimization, and also promote its applications in biological, pharmaceutical, forensic, food and environmental analyses, etc. This article is protected by copyright. All rights reserved.
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Study on the Separation Mechanism of solid‐substrate electrospray ionization mass spectrometry
Journal of separation science, 2020Co-Authors: Peiyu Shi, Bing Xia, Yan ZhouAbstract:Solid-substrate electrospray ionization mass spectrometry is an important ambient ionization technology to simplify mass spectrometry analysis. Nowadays, its Separation application has been reported increasingly, however, the detailed Separation Mechanism is still indistinct although the chromatographic effect was reported as a possible factor. In this study, substrate-filled capillary electrospray ionization mass spectrometry was developed as an ideal model to investigate the Separation Mechanism using over thirty small molecules (neutral, basic, and weakly acidic) as model compounds with C18-bonded silica gel and silica gel as the substrates. The chromatographic effect was validated by the negative t-value of oil-water distribution coefficient, and the electric field effect was verified by the paired t-test (p
Jiadao Wang - One of the best experts on this subject based on the ideXlab platform.
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Separation Mechanism and Construction of Surfaces with Special Wettability for Oil/Water Separation.
ACS applied materials & interfaces, 2019Co-Authors: Chaolang Chen, Ding Weng, Awais Mahmood, Shuai Chen, Jiadao WangAbstract:Oil leakage and the discharge of oil/water mixtures by domestic and industrial consumers have caused not only severe environmental pollution and a threat to all species in the ecosystem but also a huge waste of precious resources. Therefore, the Separation of oil/water mixtures, especially stable emulsion, has become an urgent global issue. Recently, materials containing a special wettability feature for oil and water have drawn immense attention because of their potential applications for oil/water Separation application. In this paper, we systematically summarize the fundamental theories, Separation Mechanism, design strategies, and recent developments in materials with special wettability for separating stratified and emulsified oil/water mixtures. The related wetting theories that unveil the physical underlying Mechanism of the oil/water Separation Mechanism are proposed, and the practical design criteria for oil/water Separation materials are provided. Guided by the fundamental design criteria, various porous materials with special wettability characteristics, including those which are superhydrophilic/underwater superoleophobic, superhydrophobic/superoleophilic, and superhydrophilic/in-air superoleophobic, are systemically analyzed. These superwetting materials are widely employed to separate oil/water mixtures: from stratified oil/water to emulsified ones. In addition, the materials that implement the demulsification of emulsified oil/water mixtures via the ingenious design of the multiscale surface morphology and construction of special wettability are also discussed. In each section, we introduce the design ideas, base materials, preparation methods, and representative works in detail. Finally, the conclusions and challenges for the oil/water Separation research field are discussed in depth.
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Separation Mechanism and construction of surfaces with special wettability for oil water Separation
ACS Applied Materials & Interfaces, 2019Co-Authors: Chaolang Chen, Ding Weng, Awais Mahmood, Shuai Chen, Jiadao WangAbstract:Oil leakage and the discharge of oil/water mixtures by domestic and industrial consumers have caused not only severe environmental pollution and a threat to all species in the ecosystem but also a huge waste of precious resources. Therefore, the Separation of oil/water mixtures, especially stable emulsion, has become an urgent global issue. Recently, materials containing a special wettability feature for oil and water have drawn immense attention because of their potential applications for oil/water Separation application. In this paper, we systematically summarize the fundamental theories, Separation Mechanism, design strategies, and recent developments in materials with special wettability for separating stratified and emulsified oil/water mixtures. The related wetting theories that unveil the physical underlying Mechanism of the oil/water Separation Mechanism are proposed, and the practical design criteria for oil/water Separation materials are provided. Guided by the fundamental design criteria, various porous materials with special wettability characteristics, including those which are superhydrophilic/underwater superoleophobic, superhydrophobic/superoleophilic, and superhydrophilic/in-air superoleophobic, are systemically analyzed. These superwetting materials are widely employed to separate oil/water mixtures: from stratified oil/water to emulsified ones. In addition, the materials that implement the demulsification of emulsified oil/water mixtures via the ingenious design of the multiscale surface morphology and construction of special wettability are also discussed. In each section, we introduce the design ideas, base materials, preparation methods, and representative works in detail. Finally, the conclusions and challenges for the oil/water Separation research field are discussed in depth.
Qiang Zhou - One of the best experts on this subject based on the ideXlab platform.
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phase Separation Mechanism for c terminal hyperphosphorylation of rna polymerase ii
Nature, 2018Co-Authors: Anders S Hanse, Sourav Ganguly, Rongdiao Liu, Alec Hecke, Xavie Darzacq, Qiang ZhouAbstract:Hyperphosphorylation of the C-terminal domain (CTD) of the RPB1 subunit of human RNA polymerase (Pol) II is essential for transcriptional elongation and mRNA processing1–3. The CTD contains 52 heptapeptide repeats of the consensus sequence YSPTSPS. The highly repetitive nature and abundant possible phosphorylation sites of the CTD exert special constraints on the kinases that catalyse its hyperphosphorylation. Positive transcription elongation factor b (P-TEFb)—which consists of CDK9 and cyclin T1—is known to hyperphosphorylate the CTD and negative elongation factors to stimulate Pol II elongation1,4,5. The sequence determinant on P-TEFb that facilitates this action is currently unknown. Here we identify a histidine-rich domain in cyclin T1 that promotes the hyperphosphorylation of the CTD and stimulation of transcription by CDK9. The histidine-rich domain markedly enhances the binding of P-TEFb to the CTD and functional engagement with target genes in cells. In addition to cyclin T1, at least one other kinase—DYRK1A 6 —also uses a histidine-rich domain to target and hyperphosphorylate the CTD. As a low-complexity domain, the histidine-rich domain also promotes the formation of phase-separated liquid droplets in vitro, and the localization of P-TEFb to nuclear speckles that display dynamic liquid properties and are sensitive to the disruption of weak hydrophobic interactions. The CTD—which in isolation does not phase separate, despite being a low-complexity domain—is trapped within the cyclin T1 droplets, and this process is enhanced upon pre-phosphorylation by CDK7 of transcription initiation factor TFIIH1–3. By using multivalent interactions to create a phase-separated functional compartment, the histidine-rich domain in kinases targets the CTD into this environment to ensure hyperphosphorylation and efficient elongation of Pol II. The histidine-rich domain of cyclin T1 promotes phase Separation into liquid droplets, which facilitates the hyperphosphorylation of the C-terminal domain repeats of RNA polymerase II.
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phase Separation Mechanism for c terminal hyperphosphorylation of rna polymerase ii
Nature, 2018Co-Authors: Anders S Hansen, Sourav Ganguly, Rongdiao Liu, Xavie Darzacq, Alec Heckert, Qiang ZhouAbstract:Hyperphosphorylation of the C-terminal domain (CTD) of the RPB1 subunit of human RNA polymerase (Pol) II is essential for transcriptional elongation and mRNA processing1-3. The CTD contains 52 heptapeptide repeats of the consensus sequence YSPTSPS. The highly repetitive nature and abundant possible phosphorylation sites of the CTD exert special constraints on the kinases that catalyse its hyperphosphorylation. Positive transcription elongation factor b (P-TEFb)-which consists of CDK9 and cyclin T1-is known to hyperphosphorylate the CTD and negative elongation factors to stimulate Pol II elongation1,4,5. The sequence determinant on P-TEFb that facilitates this action is currently unknown. Here we identify a histidine-rich domain in cyclin T1 that promotes the hyperphosphorylation of the CTD and stimulation of transcription by CDK9. The histidine-rich domain markedly enhances the binding of P-TEFb to the CTD and functional engagement with target genes in cells. In addition to cyclin T1, at least one other kinase-DYRK1A 6 -also uses a histidine-rich domain to target and hyperphosphorylate the CTD. As a low-complexity domain, the histidine-rich domain also promotes the formation of phase-separated liquid droplets in vitro, and the localization of P-TEFb to nuclear speckles that display dynamic liquid properties and are sensitive to the disruption of weak hydrophobic interactions. The CTD-which in isolation does not phase separate, despite being a low-complexity domain-is trapped within the cyclin T1 droplets, and this process is enhanced upon pre-phosphorylation by CDK7 of transcription initiation factor TFIIH1-3. By using multivalent interactions to create a phase-separated functional compartment, the histidine-rich domain in kinases targets the CTD into this environment to ensure hyperphosphorylation and efficient elongation of Pol II.
