The Experts below are selected from a list of 147 Experts worldwide ranked by ideXlab platform
Ross T Kelly - One of the best experts on this subject based on the ideXlab platform.
-
a simple colorful demonstration of solubility and acid base extraction using a Separatory Funnel
Journal of Chemical Education, 1993Co-Authors: Ross T KellyAbstract:A simple, colorful demonstration of solubility and acid/base extraction using a Separatory Funnel.
T. Ross Kelly - One of the best experts on this subject based on the ideXlab platform.
-
A simple, colorful demonstration of solubility and acid/base extraction using a Separatory Funnel
Journal of Chemical Education, 1993Co-Authors: T. Ross KellyAbstract:A simple, colorful demonstration of solubility and acid/base extraction using a Separatory Funnel.
Brian C. Silverman - One of the best experts on this subject based on the ideXlab platform.
-
TEACHERS TOPICS A Three-Component Extraction Using Biological Molecules: An Undergraduate Organic Experiment for Prepharmacy Students
2020Co-Authors: Robert T. Crow, Carolyn Herman, Brian C. SilvermanAbstract:Objectives. This experiment was designed for prepharmacy students to learn to use a Separatory Funnel to sequentially extract 2 components from a complex mixture, as well as develop a working knowledge of how extraction works. Design. Pairs of students used acid-base chemistry to separate a mixture of cholesterol, theophylline, and urea. The purity of the compounds was analyzed using thin layer chromatography. Student familiarity with the principles of extraction was assessed during a later laboratory examination. Assessment. All of the students used the Separatory Funnel properly. Eighty-two percent of the groups obtained pure cholesterol and 87% successfully purified theophylline. On individual examinations, 48% predicted the solubility of a molecule under either acidic or basic conditions, and 78% understood how density influences extraction. Conclusion. The overwhelming majority of students succeeded in doing the extraction properly, while a significant number retained some knowledge of the principles of extraction on a later examination.
-
A Three-Component Extraction Using Biological Molecules: An Undergraduate Organic Experiment for Prepharmacy Students
2011Co-Authors: Robert T. Crow, Carolyn Herman, Brian C. SilvermanAbstract:Objectives. This experiment was designed for prepharmacy students to learn to use a Separatory Funnel to sequentially extract 2 components from a complex mixture, as well as develop a working knowledge of how extraction works. Design. Pairs of students used acid-base chemistry to separate a mixture of cholesterol, theophylline, and urea. The purity of the compounds was analyzed using thin layer chromatography. Student familiarity with the principles of extraction was assessed during a later laboratory examination. Assessment. All of the students used the Separatory Funnel properly. Eighty-two percent of the groups obtained pure cholesterol and 87% successfully purified theophylline. On individual examinations, 48% predicted the solubility of a molecule under either acidic or basic conditions, and 78% understood how density influences extraction. Conclusion. The overwhelming majority of students succeeded in doing the extraction properly, while a significant number retained some knowledge of the principles...
Jan Sunner - One of the best experts on this subject based on the ideXlab platform.
-
Mass Spectrometric Imaging Using Laser Ablation and Solvent Capture by Aspiration (LASCA)
Journal of The American Society for Mass Spectrometry, 2015Co-Authors: Jonathan I. Brauer, Iwona B. Beech, Jan SunnerAbstract:A novel interface for ambient, laser ablation-based mass spectrometric imaging (MSI) referred to as laser ablation and solvent capture by aspiration (LASCA) is presented and its performance demonstrated using selected, unaltered biological materials. LASCA employs a pulsed 2.94 μm laser beam for specimen ablation. Ablated materials in the laser plumes are collected on a hanging solvent droplet with electric field-enhanced trapping, followed by aspiration of droplets and remaining plume material in the form of a coarse aerosol into a collection capillary. The gas and liquid phases are subsequently separated in a 10 μL-volume Separatory Funnel, and the solution is analyzed with electrospray ionization in a high mass resolution Q-ToF mass spectrometer. The LASCA system separates the sampling and ionization steps in MSI and combines high efficiencies of laser plume sampling and of electrospray ionization (ESI) with high mass resolution MS. Up to 2000 different compounds are detected from a single ablation spot (pixel). Using the LASCA platform, rapid (6 s per pixel), high sensitivity, high mass-resolution ambient imaging of “as-received” biological material is achieved routinely and reproducibly. Graphical Abstract ᅟ
D S Ningsih - One of the best experts on this subject based on the ideXlab platform.
-
Hasil Cek Similarity Total alkaloid content in various fractions of Tabernaemonata sphaerocarpa Bl. (Jembirit) leaves
2018Co-Authors: Nina Salamah, D S NingsihAbstract:Tabernaemontana sphaerocarpa Bl. (Jembirit) is one of the Apocynaceae family plants containing alkaloid compound. Traditionally, it is used as an anti-inflammatory medicine. It is found to have a new bisindole alkaloid compound that shows a potent cytotoxic activity in human cancer. This study aimed to know the total alkaloid content in some fractions of ethanolic extract of T. sphaerocarpa Bl. leaf powder was extracted by maceration method in 70% ethanol solvent. Then, the extract was fractionated in a Separatory Funnel using water, ethyl acetate, and hexane. The total alkaloid content in each fraction was analyzed with visible spectrophotometric methods based on the reaction with Bromocresol Green (BCG). The total alkaloids in water fraction and ethyl acetate fraction were (0.0312±0.0009)% and (0.0281±0.0014)%, respectively. Meanwhile, the total alkaloid content in hexane was not detected. The statistical analysis, performed in SPSS, resulted in a significant difference between the total alkaloids in water fraction and ethyl acetate fraction. The total alkaloid in water fraction of T. sphaerocarpa Bl. was higher than the one in ethyl acetate fraction.
