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Yijun Ruan - One of the best experts on this subject based on the ideXlab platform.
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multiplex Sequencing of paired end ditags ms pet a strategy for the ultra high throughput analysis of transcriptomes and genomes
Nucleic Acids Research, 2006Co-Authors: P C Ng, Kuo Ping Chiu, Melissa J Fullwood, Kandhadayar G Srinivasan, Clotilde Perbost, Lei Du, Wingkin Sung, Yijun RuanAbstract:The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based Sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex Sequencing Method (454-Sequencing TM ) using picolitrescale reactions has achieved a remarkable advance in efficiency, but suffers from short-read lengths, and a lack of paired-end information. To further enhance the efficiency of PET analysis and at the same time overcome the drawbacks of the new Sequencing Method, we coupled multiplex Sequencing with paired-end ditagging (MS-PET) using modified PET procedures to simultaneously sequence 200 000 to 300 000 dimerized PET (diPET) templates, with an output of nearly half-a-million PET sequences in a single 4 h machine run. We demonstrate the utility and robustness of MS-PET by analyzing the transcriptome of human breast carcinoma cells, and by mapping p53 binding sites in the genome of human colorectal carcinoma cells. This combined Sequencing strategy achieved an approximate 100-fold efficiency increase over the current standard for PET analysis, and furthermore enables the short-read-length multiplex Sequencing procedure to acquire paired-end information from large DNA fragments.
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multiplex Sequencing of paired end ditags ms pet a strategy for the ultra high throughput analysis of transcriptomes and genomes
Nucleic Acids Research, 2006Co-Authors: Jack J S Tan, Kuo Ping Chiu, Melissa J Fullwood, Kandhadayar G Srinivasan, Clotilde Perbost, Wingkin Sung, Hong Sain Ooi, Yen Ling Lee, Chialin Wei, Yijun RuanAbstract:The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based Sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex Sequencing Method (454-Sequencing TM ) using picolitrescale reactions has achieved a remarkable advance in efficiency, but suffers from short-read lengths, and a lack of paired-end information. To further enhance the efficiency of PET analysis and at the same time overcome the drawbacks of the new Sequencing Method, we coupled multiplex Sequencing with paired-end ditagging (MS-PET) using modified PET procedures to simultaneously sequence 200 000 to 300 000 dimerized PET (diPET) templates, with an output of nearly half-a-million PET sequences in a single 4 h machine run. We demonstrate the utility and robustness of MS-PET by analyzing the transcriptome of human breast carcinoma cells, and by mapping p53 binding sites in the genome of human colorectal carcinoma cells. This combined Sequencing strategy achieved an approximate 100-fold efficiency increase over the current standard for PET analysis, and furthermore enables the short-read-length multiplex Sequencing procedure to acquire paired-end information from large DNA fragments.
Kuo Ping Chiu - One of the best experts on this subject based on the ideXlab platform.
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multiplex Sequencing of paired end ditags ms pet a strategy for the ultra high throughput analysis of transcriptomes and genomes
Nucleic Acids Research, 2006Co-Authors: P C Ng, Kuo Ping Chiu, Melissa J Fullwood, Kandhadayar G Srinivasan, Clotilde Perbost, Lei Du, Wingkin Sung, Yijun RuanAbstract:The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based Sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex Sequencing Method (454-Sequencing TM ) using picolitrescale reactions has achieved a remarkable advance in efficiency, but suffers from short-read lengths, and a lack of paired-end information. To further enhance the efficiency of PET analysis and at the same time overcome the drawbacks of the new Sequencing Method, we coupled multiplex Sequencing with paired-end ditagging (MS-PET) using modified PET procedures to simultaneously sequence 200 000 to 300 000 dimerized PET (diPET) templates, with an output of nearly half-a-million PET sequences in a single 4 h machine run. We demonstrate the utility and robustness of MS-PET by analyzing the transcriptome of human breast carcinoma cells, and by mapping p53 binding sites in the genome of human colorectal carcinoma cells. This combined Sequencing strategy achieved an approximate 100-fold efficiency increase over the current standard for PET analysis, and furthermore enables the short-read-length multiplex Sequencing procedure to acquire paired-end information from large DNA fragments.
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multiplex Sequencing of paired end ditags ms pet a strategy for the ultra high throughput analysis of transcriptomes and genomes
Nucleic Acids Research, 2006Co-Authors: Jack J S Tan, Kuo Ping Chiu, Melissa J Fullwood, Kandhadayar G Srinivasan, Clotilde Perbost, Wingkin Sung, Hong Sain Ooi, Yen Ling Lee, Chialin Wei, Yijun RuanAbstract:The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based Sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex Sequencing Method (454-Sequencing TM ) using picolitrescale reactions has achieved a remarkable advance in efficiency, but suffers from short-read lengths, and a lack of paired-end information. To further enhance the efficiency of PET analysis and at the same time overcome the drawbacks of the new Sequencing Method, we coupled multiplex Sequencing with paired-end ditagging (MS-PET) using modified PET procedures to simultaneously sequence 200 000 to 300 000 dimerized PET (diPET) templates, with an output of nearly half-a-million PET sequences in a single 4 h machine run. We demonstrate the utility and robustness of MS-PET by analyzing the transcriptome of human breast carcinoma cells, and by mapping p53 binding sites in the genome of human colorectal carcinoma cells. This combined Sequencing strategy achieved an approximate 100-fold efficiency increase over the current standard for PET analysis, and furthermore enables the short-read-length multiplex Sequencing procedure to acquire paired-end information from large DNA fragments.
Wingkin Sung - One of the best experts on this subject based on the ideXlab platform.
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multiplex Sequencing of paired end ditags ms pet a strategy for the ultra high throughput analysis of transcriptomes and genomes
Nucleic Acids Research, 2006Co-Authors: P C Ng, Kuo Ping Chiu, Melissa J Fullwood, Kandhadayar G Srinivasan, Clotilde Perbost, Lei Du, Wingkin Sung, Yijun RuanAbstract:The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based Sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex Sequencing Method (454-Sequencing TM ) using picolitrescale reactions has achieved a remarkable advance in efficiency, but suffers from short-read lengths, and a lack of paired-end information. To further enhance the efficiency of PET analysis and at the same time overcome the drawbacks of the new Sequencing Method, we coupled multiplex Sequencing with paired-end ditagging (MS-PET) using modified PET procedures to simultaneously sequence 200 000 to 300 000 dimerized PET (diPET) templates, with an output of nearly half-a-million PET sequences in a single 4 h machine run. We demonstrate the utility and robustness of MS-PET by analyzing the transcriptome of human breast carcinoma cells, and by mapping p53 binding sites in the genome of human colorectal carcinoma cells. This combined Sequencing strategy achieved an approximate 100-fold efficiency increase over the current standard for PET analysis, and furthermore enables the short-read-length multiplex Sequencing procedure to acquire paired-end information from large DNA fragments.
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multiplex Sequencing of paired end ditags ms pet a strategy for the ultra high throughput analysis of transcriptomes and genomes
Nucleic Acids Research, 2006Co-Authors: Jack J S Tan, Kuo Ping Chiu, Melissa J Fullwood, Kandhadayar G Srinivasan, Clotilde Perbost, Wingkin Sung, Hong Sain Ooi, Yen Ling Lee, Chialin Wei, Yijun RuanAbstract:The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based Sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex Sequencing Method (454-Sequencing TM ) using picolitrescale reactions has achieved a remarkable advance in efficiency, but suffers from short-read lengths, and a lack of paired-end information. To further enhance the efficiency of PET analysis and at the same time overcome the drawbacks of the new Sequencing Method, we coupled multiplex Sequencing with paired-end ditagging (MS-PET) using modified PET procedures to simultaneously sequence 200 000 to 300 000 dimerized PET (diPET) templates, with an output of nearly half-a-million PET sequences in a single 4 h machine run. We demonstrate the utility and robustness of MS-PET by analyzing the transcriptome of human breast carcinoma cells, and by mapping p53 binding sites in the genome of human colorectal carcinoma cells. This combined Sequencing strategy achieved an approximate 100-fold efficiency increase over the current standard for PET analysis, and furthermore enables the short-read-length multiplex Sequencing procedure to acquire paired-end information from large DNA fragments.
Melissa J Fullwood - One of the best experts on this subject based on the ideXlab platform.
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multiplex Sequencing of paired end ditags ms pet a strategy for the ultra high throughput analysis of transcriptomes and genomes
Nucleic Acids Research, 2006Co-Authors: P C Ng, Kuo Ping Chiu, Melissa J Fullwood, Kandhadayar G Srinivasan, Clotilde Perbost, Lei Du, Wingkin Sung, Yijun RuanAbstract:The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based Sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex Sequencing Method (454-Sequencing TM ) using picolitrescale reactions has achieved a remarkable advance in efficiency, but suffers from short-read lengths, and a lack of paired-end information. To further enhance the efficiency of PET analysis and at the same time overcome the drawbacks of the new Sequencing Method, we coupled multiplex Sequencing with paired-end ditagging (MS-PET) using modified PET procedures to simultaneously sequence 200 000 to 300 000 dimerized PET (diPET) templates, with an output of nearly half-a-million PET sequences in a single 4 h machine run. We demonstrate the utility and robustness of MS-PET by analyzing the transcriptome of human breast carcinoma cells, and by mapping p53 binding sites in the genome of human colorectal carcinoma cells. This combined Sequencing strategy achieved an approximate 100-fold efficiency increase over the current standard for PET analysis, and furthermore enables the short-read-length multiplex Sequencing procedure to acquire paired-end information from large DNA fragments.
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multiplex Sequencing of paired end ditags ms pet a strategy for the ultra high throughput analysis of transcriptomes and genomes
Nucleic Acids Research, 2006Co-Authors: Jack J S Tan, Kuo Ping Chiu, Melissa J Fullwood, Kandhadayar G Srinivasan, Clotilde Perbost, Wingkin Sung, Hong Sain Ooi, Yen Ling Lee, Chialin Wei, Yijun RuanAbstract:The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based Sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex Sequencing Method (454-Sequencing TM ) using picolitrescale reactions has achieved a remarkable advance in efficiency, but suffers from short-read lengths, and a lack of paired-end information. To further enhance the efficiency of PET analysis and at the same time overcome the drawbacks of the new Sequencing Method, we coupled multiplex Sequencing with paired-end ditagging (MS-PET) using modified PET procedures to simultaneously sequence 200 000 to 300 000 dimerized PET (diPET) templates, with an output of nearly half-a-million PET sequences in a single 4 h machine run. We demonstrate the utility and robustness of MS-PET by analyzing the transcriptome of human breast carcinoma cells, and by mapping p53 binding sites in the genome of human colorectal carcinoma cells. This combined Sequencing strategy achieved an approximate 100-fold efficiency increase over the current standard for PET analysis, and furthermore enables the short-read-length multiplex Sequencing procedure to acquire paired-end information from large DNA fragments.
Kandhadayar G Srinivasan - One of the best experts on this subject based on the ideXlab platform.
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multiplex Sequencing of paired end ditags ms pet a strategy for the ultra high throughput analysis of transcriptomes and genomes
Nucleic Acids Research, 2006Co-Authors: P C Ng, Kuo Ping Chiu, Melissa J Fullwood, Kandhadayar G Srinivasan, Clotilde Perbost, Lei Du, Wingkin Sung, Yijun RuanAbstract:The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based Sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex Sequencing Method (454-Sequencing TM ) using picolitrescale reactions has achieved a remarkable advance in efficiency, but suffers from short-read lengths, and a lack of paired-end information. To further enhance the efficiency of PET analysis and at the same time overcome the drawbacks of the new Sequencing Method, we coupled multiplex Sequencing with paired-end ditagging (MS-PET) using modified PET procedures to simultaneously sequence 200 000 to 300 000 dimerized PET (diPET) templates, with an output of nearly half-a-million PET sequences in a single 4 h machine run. We demonstrate the utility and robustness of MS-PET by analyzing the transcriptome of human breast carcinoma cells, and by mapping p53 binding sites in the genome of human colorectal carcinoma cells. This combined Sequencing strategy achieved an approximate 100-fold efficiency increase over the current standard for PET analysis, and furthermore enables the short-read-length multiplex Sequencing procedure to acquire paired-end information from large DNA fragments.
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multiplex Sequencing of paired end ditags ms pet a strategy for the ultra high throughput analysis of transcriptomes and genomes
Nucleic Acids Research, 2006Co-Authors: Jack J S Tan, Kuo Ping Chiu, Melissa J Fullwood, Kandhadayar G Srinivasan, Clotilde Perbost, Wingkin Sung, Hong Sain Ooi, Yen Ling Lee, Chialin Wei, Yijun RuanAbstract:The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based Sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex Sequencing Method (454-Sequencing TM ) using picolitrescale reactions has achieved a remarkable advance in efficiency, but suffers from short-read lengths, and a lack of paired-end information. To further enhance the efficiency of PET analysis and at the same time overcome the drawbacks of the new Sequencing Method, we coupled multiplex Sequencing with paired-end ditagging (MS-PET) using modified PET procedures to simultaneously sequence 200 000 to 300 000 dimerized PET (diPET) templates, with an output of nearly half-a-million PET sequences in a single 4 h machine run. We demonstrate the utility and robustness of MS-PET by analyzing the transcriptome of human breast carcinoma cells, and by mapping p53 binding sites in the genome of human colorectal carcinoma cells. This combined Sequencing strategy achieved an approximate 100-fold efficiency increase over the current standard for PET analysis, and furthermore enables the short-read-length multiplex Sequencing procedure to acquire paired-end information from large DNA fragments.
