The Experts below are selected from a list of 75 Experts worldwide ranked by ideXlab platform
Balasubramanian Revathi - One of the best experts on this subject based on the ideXlab platform.
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3D modeling of esophageal development using human PSC-Derived basal progenitors reveals a critical role for notch signaling
Elsevier, 2018Co-Authors: Zhang Yongchun, Yang Ying, Jiang Ming, Huang, Sarah Xuelian, Zhang Wanwei, Al Alam Denise, Danopoulos Soula, Mori Munemasa, Chen Ya-wen, Balasubramanian RevathiAbstract:Pluripotent stem cells (PSCs) could provide a powerful system to model development of the human esophagus, whose distinct tissue organization compared to rodent esophagus suggests that developmental mechanisms may not be conserved between species. We therefore established an efficient protocol for generating esophageal progenitor cells (EPCs) from human PSCs. We found that inhibition of TGF-ß and BMP signaling is required for Sequential Specification of EPCs, which can be further purified using cell-surface markers. These EPCs resemble their human fetal counterparts and can recapitulate normal development of esophageal stratified squamous epithelium during in vitro 3D cultures and in vivo. Importantly, combining hPSC differentiation strategies with mouse genetics elucidated a critical role for Notch signaling in the formation of this epithelium. These studies therefore not only provide an efficient approach to generate EPCs, but also offer a model system to study the regulatory mechanisms underlying development of the human esophagus.Work in the Que lab is partly supported by R01DK113144, R01DK100342, and R01HL132996 (J.Q.), and the Price Family Foundation. S.D. acknowledges a fellowship support from the Hastings Center for Pulmonary Research (USC). Flow cytometry was performed in the Columbia Center for Translational Immunology (CCTI) Flow Cytometry Core at Columbia University Medical Center, supported in part by the Office of the Director, NIH under the award S10OD020056.info:eu-repo/semantics/publishedVersio
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3D modeling of esophageal development using human PSC-derived basal progenitors reveals a critical role for notch signaling
'Elsevier BV', 2018Co-Authors: Zhang Yongchun, Yang Ying, Jiang Ming, Huang, Sarah Xuelian, Zhang Wanwei, Al Alam Denise, Danopoulos Soula, Mori Munemasa, Chen Ya-wen, Balasubramanian RevathiAbstract:Pluripotent stem cells (PSCs) could provide a powerful system to model development of the human esophagus, whose distinct tissue organization compared to rodent esophagus suggests that developmental mechanisms may not be conserved between species. We therefore established an efficient protocol for generating esophageal progenitor cells (EPCs) from human PSCs. We found that inhibition of TGF beta and BMP signaling is required for Sequential Specification of EPCs, which can be further purified using cell-surface markers. These EPCs resemble their human fetal counterparts and can recapitulate normal development of esophageal stratified squamous epithelium during in vitro 3D cultures and in vivo. Importantly, combining hPSC differentiation strategies with mouse genetics elucidated a critical role for Notch signaling in the formation of this epithelium. These studies therefore not only provide an efficient approach to generate EPCs, but also offer a model system to study the regulatory mechanisms underlying development of the human esophagus
Zhang Yongchun - One of the best experts on this subject based on the ideXlab platform.
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3D modeling of esophageal development using human PSC-Derived basal progenitors reveals a critical role for notch signaling
Elsevier, 2018Co-Authors: Zhang Yongchun, Yang Ying, Jiang Ming, Huang, Sarah Xuelian, Zhang Wanwei, Al Alam Denise, Danopoulos Soula, Mori Munemasa, Chen Ya-wen, Balasubramanian RevathiAbstract:Pluripotent stem cells (PSCs) could provide a powerful system to model development of the human esophagus, whose distinct tissue organization compared to rodent esophagus suggests that developmental mechanisms may not be conserved between species. We therefore established an efficient protocol for generating esophageal progenitor cells (EPCs) from human PSCs. We found that inhibition of TGF-ß and BMP signaling is required for Sequential Specification of EPCs, which can be further purified using cell-surface markers. These EPCs resemble their human fetal counterparts and can recapitulate normal development of esophageal stratified squamous epithelium during in vitro 3D cultures and in vivo. Importantly, combining hPSC differentiation strategies with mouse genetics elucidated a critical role for Notch signaling in the formation of this epithelium. These studies therefore not only provide an efficient approach to generate EPCs, but also offer a model system to study the regulatory mechanisms underlying development of the human esophagus.Work in the Que lab is partly supported by R01DK113144, R01DK100342, and R01HL132996 (J.Q.), and the Price Family Foundation. S.D. acknowledges a fellowship support from the Hastings Center for Pulmonary Research (USC). Flow cytometry was performed in the Columbia Center for Translational Immunology (CCTI) Flow Cytometry Core at Columbia University Medical Center, supported in part by the Office of the Director, NIH under the award S10OD020056.info:eu-repo/semantics/publishedVersio
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3D modeling of esophageal development using human PSC-derived basal progenitors reveals a critical role for notch signaling
'Elsevier BV', 2018Co-Authors: Zhang Yongchun, Yang Ying, Jiang Ming, Huang, Sarah Xuelian, Zhang Wanwei, Al Alam Denise, Danopoulos Soula, Mori Munemasa, Chen Ya-wen, Balasubramanian RevathiAbstract:Pluripotent stem cells (PSCs) could provide a powerful system to model development of the human esophagus, whose distinct tissue organization compared to rodent esophagus suggests that developmental mechanisms may not be conserved between species. We therefore established an efficient protocol for generating esophageal progenitor cells (EPCs) from human PSCs. We found that inhibition of TGF beta and BMP signaling is required for Sequential Specification of EPCs, which can be further purified using cell-surface markers. These EPCs resemble their human fetal counterparts and can recapitulate normal development of esophageal stratified squamous epithelium during in vitro 3D cultures and in vivo. Importantly, combining hPSC differentiation strategies with mouse genetics elucidated a critical role for Notch signaling in the formation of this epithelium. These studies therefore not only provide an efficient approach to generate EPCs, but also offer a model system to study the regulatory mechanisms underlying development of the human esophagus
Marco J G Bekooij - One of the best experts on this subject based on the ideXlab platform.
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Sequential Specification of time aware stream processing applications
ACM Transactions in Embedded Computing Systems, 2013Co-Authors: Stefan J Geuns, Joost P H M Hausmans, Marco J G BekooijAbstract:Automatic parallelization of Nested Loop Programs (NLPs) is an attractive method to create embedded real-time stream processing applications for multi-core systems. However, the description and parallelization of applications with a time dependent functional behavior has not been considered in NLPs. In such a description, semantic information about time dependent behavior must be made available for the compiler, such that an optimized time independent implementation can be generated automatically. This article introduces language constructs with temporal semantics to NLPs. Using these language constructs, time dependent applications can be specified and a corresponding data-driven implementation can be generated for use on a multi-core system. Despite that these time-aware language constructs can be data-dependent, the application remains functionally deterministic. Pipelining is exploited to increase the throughput of an application. The media access control (MAC) protocol of an IEEE 802.11p WLAN transceiver is used to illustrate the relevance and applicability of the introduced concepts.
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Sequential Specification of time aware stream processing applications extended abstract
Embedded Systems for Real-Time Multimedia, 2012Co-Authors: Stefan J Geuns, Joost P H M Hausmans, Marco J G BekooijAbstract:Stream processing applications, and in particular Software Defined Radio applications, are typically executed on multi-core systems. Such applications often have real-time throughput constraints. Automatic parallelization of Nested Loop Programs (NLPs) is an attractive method to create embedded real-time stream processing applications for multi-core systems [1]. However, the description and parallelization of applications with a time dependent functional behavior has not been considered for NLPs. In such a description, semantic information about time dependent behavior must be made available for the compiler, such that an optimized time independent implementation can be generated automatically.
Chris Q Doe - One of the best experts on this subject based on the ideXlab platform.
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drosophila neuroblast 7 3 cell lineage a model system for studying programmed cell death notch numb signaling and Sequential Specification of ganglion mother cell identity
The Journal of Comparative Neurology, 2005Co-Authors: Rachel Karcavich, Chris Q DoeAbstract:Cell lineage studies provide an important foundation for experimental analysis in many systems. Drosophila neural precursors (neuroblasts) Sequentially generate ganglion mother cells (GMCs), which generate neurons and/or glia, but the birth order, or cell lineage, of each neuroblast is poorly understood. The best-characterized neuroblast is NB7-3, in which GMC-1 makes the EW1 serotonergic interneuron and GW motoneuron; GMC-2 makes the EW2 serotonergic interneuron and a programmed cell death; and GMC-3 gives rise to the EW3 interneuron. However, the end of this lineage has not been determined. Here, we use positively marked genetic clones, bromodeoxyuridine (BrdU) labeling, mutations that affect Notch signaling, and antibody markers to further define the end of the cell lineage of NB7-3. We provide evidence that GMC-3 directly differentiates into EW3 and that the sibling neuroblast undergoes programmed cell death. Our results confirm and extend previous work on the early portion of the NB7-3 lineage (Novotny et al. [2002] Development 129:1027–1036; Lundell et al. [2003] Development 130:4109 – 4121). J. Comp. Neurol. 481:240 –251, 2005. © 2004 Wiley-Liss, Inc. Indexing terms: cell lineage; neuroblast; programmed cell death; Notch; sanpodo; numb; serotonin; corazonin; NB7-3
Yang Ying - One of the best experts on this subject based on the ideXlab platform.
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3D modeling of esophageal development using human PSC-Derived basal progenitors reveals a critical role for notch signaling
Elsevier, 2018Co-Authors: Zhang Yongchun, Yang Ying, Jiang Ming, Huang, Sarah Xuelian, Zhang Wanwei, Al Alam Denise, Danopoulos Soula, Mori Munemasa, Chen Ya-wen, Balasubramanian RevathiAbstract:Pluripotent stem cells (PSCs) could provide a powerful system to model development of the human esophagus, whose distinct tissue organization compared to rodent esophagus suggests that developmental mechanisms may not be conserved between species. We therefore established an efficient protocol for generating esophageal progenitor cells (EPCs) from human PSCs. We found that inhibition of TGF-ß and BMP signaling is required for Sequential Specification of EPCs, which can be further purified using cell-surface markers. These EPCs resemble their human fetal counterparts and can recapitulate normal development of esophageal stratified squamous epithelium during in vitro 3D cultures and in vivo. Importantly, combining hPSC differentiation strategies with mouse genetics elucidated a critical role for Notch signaling in the formation of this epithelium. These studies therefore not only provide an efficient approach to generate EPCs, but also offer a model system to study the regulatory mechanisms underlying development of the human esophagus.Work in the Que lab is partly supported by R01DK113144, R01DK100342, and R01HL132996 (J.Q.), and the Price Family Foundation. S.D. acknowledges a fellowship support from the Hastings Center for Pulmonary Research (USC). Flow cytometry was performed in the Columbia Center for Translational Immunology (CCTI) Flow Cytometry Core at Columbia University Medical Center, supported in part by the Office of the Director, NIH under the award S10OD020056.info:eu-repo/semantics/publishedVersio
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3D modeling of esophageal development using human PSC-derived basal progenitors reveals a critical role for notch signaling
'Elsevier BV', 2018Co-Authors: Zhang Yongchun, Yang Ying, Jiang Ming, Huang, Sarah Xuelian, Zhang Wanwei, Al Alam Denise, Danopoulos Soula, Mori Munemasa, Chen Ya-wen, Balasubramanian RevathiAbstract:Pluripotent stem cells (PSCs) could provide a powerful system to model development of the human esophagus, whose distinct tissue organization compared to rodent esophagus suggests that developmental mechanisms may not be conserved between species. We therefore established an efficient protocol for generating esophageal progenitor cells (EPCs) from human PSCs. We found that inhibition of TGF beta and BMP signaling is required for Sequential Specification of EPCs, which can be further purified using cell-surface markers. These EPCs resemble their human fetal counterparts and can recapitulate normal development of esophageal stratified squamous epithelium during in vitro 3D cultures and in vivo. Importantly, combining hPSC differentiation strategies with mouse genetics elucidated a critical role for Notch signaling in the formation of this epithelium. These studies therefore not only provide an efficient approach to generate EPCs, but also offer a model system to study the regulatory mechanisms underlying development of the human esophagus
